BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0024.0001.p (381 letters) Database: COG 115,334 sequences; 38,378,431 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value MTH1188 [M] COG0399 Predicted pyridoxal phosphate-dependent enzy... 202 8e-52 CC1012 [M] COG0399 Predicted pyridoxal phosphate-dependent enzym... 191 2e-48 MTH334 [M] COG0399 Predicted pyridoxal phosphate-dependent enzym... 189 7e-48 RSp1006 [M] COG0399 Predicted pyridoxal phosphate-dependent enzy... 189 8e-48 slr2114 [M] COG0399 Predicted pyridoxal phosphate-dependent enzy... 185 9e-47 >MTH1188 [M] COG0399 Predicted pyridoxal phosphate-dependent enzyme apparently involved in regulation of cell wall biogenesis Length = 176 Score = 202 bits (513), Expect = 8e-52, Method: Compositional matrix adjust. Identities = 0/350 (0%), Positives = 0/350 (0%), Gaps = 22/350 (6%) Query: 24 GTKVKEFERVFAEMHNAKYALGVTNGTNAIDLLLKSFKVKEGDEVIVPAFTFISTALPLM 83 + E + S D ++ + ++ Sbjct: 29 IKYEIDKESGALFVDRFMSTAMFYPCNYGYINHTLSLDGDPVDVLVPTPYPLQPGSVIRC 88 Query: 84 SLGAIPVPVDIDPGTFCLDPEKVREAVTERTVGIIPVHMAGHLCDMDRLLDIASEFNLFI 143 + D L + E + L + Sbjct: 89 RPVGVLKMTDEAGEDAKLVAVPHSKLSKEYDHIKDVNDLPELLKAQIAHFFEHYKDLEKG 148 Query: 144 MEDACHAHGGEWKGKRAGSIGDAGVFSFQQFKLMTAGEGGALITNSQELYDQAFLYHNVG 203 Sbjct: 149 208 Query: 204 RPFGDKKYQHLVEGTNYRFSEFQAAVLLPQAGRLAEQNQRRERNAQILNEEIQTIEGIVP 263 Sbjct: 209 ---- 264 Query: 264 QGRLEACTLHTHYMYMFYYNPGKFGGISRERFVEMLNEQGIPAYIAYAQIHDTPIFKEFA 323 Sbjct: 265 ------------ 312 Query: 324 ASLDDSYSFPNQLDCPASKKVAEEVIWIH-HRALLGDEEATRGIAQTIRE 372 Sbjct: 313 ----- 357 >CC1012 [M] COG0399 Predicted pyridoxal phosphate-dependent enzyme apparently involved in regulation of cell wall biogenesis Length = 176 Score = 191 bits (485), Expect = 2e-48, Method: Compositional matrix adjust. Identities = 0/305 (0%), Positives = 0/305 (0%), Gaps = 3/305 (0%) Query: 19 WWRMTGTKVKEFERVFAEMHNAKYALGVTNGTNAIDLLLKSFKVKEGDEVIVPAFTFIST 78 + + F F + + P Sbjct: 30 KYEIDKESGALFVDRFMSTAMFYPCNYGYINHTLSLDGDPVDVLVPTPYPLQPGSVIRCR 89 Query: 79 ALPLMSLGAIPVPVDIDPGTFCLDPEKVREAVTERTVGIIPVHMAGHLCDMDRLLDIASE 138 + ++ + K + + + + + Sbjct: 90 PVGVLKMTDEAGEDAKLVAVPHSKLSKEYDHIKDVNDLPELLKAQIAHFFEHYKDLEKGK 149 Query: 139 FNLFIMEDACHAHGGEWKGKRAGSIGDAGVFSFQQFKLMTAGEGGALITNSQELYDQAFL 198 Sbjct: 150 209 Query: 199 YHNVGRPFGDKKYQHLVEGTNYRFSEFQAAVLLPQAGRLAEQNQRRERNAQILNEEIQTI 258 Sbjct: 210 - 268 Query: 259 EGIVPQGRLEACTLHTHYMYMFYYNPGKFGGISRERFVEMLNEQGIPAYIAYAQIHDTPI 318 Sbjct: 269 -- 326 Query: 319 FKEFA 323 Sbjct: 327 331 >MTH334 [M] COG0399 Predicted pyridoxal phosphate-dependent enzyme apparently involved in regulation of cell wall biogenesis Length = 176 Score = 189 bits (480), Expect = 7e-48, Method: Compositional matrix adjust. Identities = 0/340 (0%), Positives = 0/340 (0%), Gaps = 22/340 (6%) Query: 19 WWRMTGTKVKEFERVFAEMHNAKYALGVTNGTNAIDLLLKSFKVKEGDEVIVPAFTFIST 78 ++ + A+ I+ L V P + Sbjct: 25 NADPIKYEIDKESGALFVDRFMSTAMFYPCNYGYINHTLSLDGDPVDVLVPTPYPLQPGS 84 Query: 79 ALPLMSLGAIPVPVDIDPGTFCLDPEKVREAVTERTVGIIPVHMAGHLCDMDRLLDIASE 138 + +G + + + + + + + + + + + Sbjct: 85 VIRCRPVGVLKMTDEAGEDAKLVAVPHSKLSKEYDHIKDVNDLPELLKAQIAHFFEHYKD 144 Query: 139 FNLFIMEDACHAHGGEWKGKRAGSIGDAGVFSFQQFKLMTAGEGGALITNSQELYDQAFL 198 Sbjct: 145 204 Query: 199 YHNVG-RPFGDKKYQHLVEGTNYRFSEFQAAVLLPQAGRLAEQNQRRERNAQILNEEIQT 257 Sbjct: 205 ------- 257 Query: 258 IEGIVPQGRLEACTLHTHYMY---MFYYNPGKFGGISRERFVEMLNEQGIPAYIAYAQIH 314 Sbjct: 258 - 316 Query: 315 DTPIFKEFAASLDDSYSFPNQ-----LDCPASKKVAEEVI 349 Sbjct: 317 ----- 351 >RSp1006 [M] COG0399 Predicted pyridoxal phosphate-dependent enzyme apparently involved in regulation of cell wall biogenesis Length = 176 Score = 189 bits (479), Expect = 8e-48, Method: Compositional matrix adjust. Identities = 0/303 (0%), Positives = 0/303 (0%), Gaps = 17/303 (5%) Query: 17 NQWWRMTGTKVKEFERVFAEMHNAKYALGVTNGTNAIDLLLKSFKVKEGDEVIVPAFTFI 76 + Y + + L V + + I Sbjct: 30 KYE---IDKESGALFVDRFMSTAMFYPCNYGYINHTLSLDGDPVDVLVPTPYPLQPGSVI 86 Query: 77 STALPLMSLGAIPVPVDIDPGTFCLDPEKVREAVTERTVGIIPVHMAGHLCDMDRLLDIA 136 + D + + + A + D+ Sbjct: 87 RCRPVGVLKMTDEAGEDAKLVAVPHSKLSKEYDHIKDVNDLPELLKAQIAHFFEHYKDLE 146 Query: 137 SEFNLFIMEDACHAHGGEWKGKRAGSIGDAGVFSFQQFKLM-TAGEGGALITNSQELYDQ 195 Sbjct: 147 206 Query: 196 AFLYHNVGR----------PFGDKKYQHLVEGTNYRFSEFQAAVLLPQAGRLAEQNQRRE 245 Sbjct: 207 - 265 Query: 246 RNAQILNEEIQTIEGIVPQGRLEACTLHTHYMYMFYYNPGKF-GGISRERFVEMLNEQGI 304 Sbjct: 266 - 324 Query: 305 PAY 307 Sbjct: 325 327 >slr2114 [M] COG0399 Predicted pyridoxal phosphate-dependent enzyme apparently involved in regulation of cell wall biogenesis Length = 176 Score = 185 bits (470), Expect = 9e-47, Method: Compositional matrix adjust. Identities = 0/287 (0%), Positives = 0/287 (0%), Gaps = 3/287 (1%) Query: 33 VFAEMHNAKYALGVTNGTNAIDLLLKSFKVKEGDEVIVPAFTFISTALPLMSLGAIPVPV 92 + + + + A+ + +S + + Sbjct: 20 IEIPANADPIKYEIDKESGALFVDRFMSTAMFYPCNYGYINHTLSLDGDPVDVLVPTPYP 79 Query: 93 DIDPGTFCLDPEKVREAVTERTVGIIPVHMAGHLCDMDRLLDIASEFNLFIMEDACHAHG 152 P V + E V + + +++ Sbjct: 80 LQPGSVIRCRPVGVLKMTDEAGEDAKLVAVPHSKLSKEYDHIKDVNDLPELLKAQIAHFF 139 Query: 153 GEWKGKRAGSIGDAGVFSFQQFKLMTAGEGGALITNSQELYDQAFLYHNVGRPFGDKKYQ 212 Sbjct: 140 - 198 Query: 213 HLVEGTNYRFSEFQAAVLLPQAGRLAEQNQRRERNAQILNEEIQTIEGIVPQGRLEACTL 272 Sbjct: 199 - 257 Query: 273 HTHYMYMFYYNPGKFGGISRERFVEMLNEQGIPAYIAYAQIHDTPIF 319 Sbjct: 258 - 303 Database: COG Posted date: Aug 3, 2010 9:58 AM Number of letters in database: 38,378,431 Number of sequences in database: 115,334 Lambda K H 0.321 0.137 0.412 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 115334 Number of Hits to DB: 27,901,131 Number of extensions: 1151273 Number of successful extensions: 2160 Number of sequences better than 1.0e-05: 52 Number of HSP's gapped: 2089 Number of HSP's successfully gapped: 52 Length of query: 381 Length of database: 38,378,431 Length adjustment: 108 Effective length of query: 273 Effective length of database: 25,922,359 Effective search space: 7076804007 Effective search space used: 7076804007 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.4 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.9 bits) S2: 117 (49.7 bits)