BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0023.0006.p (227 letters) Database: COG 115,334 sequences; 38,378,431 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value BS_ywbG [M] COG1346 Putative effector of murein hydrolase 233 2e-61 PA3431 [M] COG1346 Putative effector of murein hydrolase 149 2e-36 VC2100 [M] COG1346 Putative effector of murein hydrolase 138 6e-33 SA2328 [M] COG1346 Putative effector of murein hydrolase 129 3e-30 FN0468 [M] COG1346 Putative effector of murein hydrolase 125 6e-29 >BS_ywbG [M] COG1346 Putative effector of murein hydrolase Length = 241 Score = 233 bits (594), Expect = 2e-61, Method: Compositional matrix adjust. Identities = 117/202 (57%), Positives = 150/202 (74%) Query: 18 AKRIYASSSKMYASXXXXXXXXXXXXXXXXXXXYESYNAGGKWLTDLLQPATIAFAIPLH 77 AK++Y ++Y S YESYN GG LTD+LQPAT+AFAIPL+ Sbjct: 18 AKKVYQRFPRVYTSPLLVTPAVLVGLLLLVNVPYESYNLGGGLLTDMLQPATVAFAIPLY 77 Query: 78 KNFKVLKKHAAEIAAGVLSGTVMAVLSSMLLAKWLHLSGDLATSLVPRSVTTPIAMSISQ 137 K F VLKK+A EI V G+ +A++S+ L+AKWLHL L SLVPRSVTTPIA+ +S+ Sbjct: 78 KYFPVLKKYAVEIILNVAVGSCIAIISTALIAKWLHLGTGLIDSLVPRSVTTPIAVHVSE 137 Query: 138 SIGGVPSITAVFVILTGVLGTLMGPSVLRLFRIDNEIARGVSLGTAAHGTGTSKAFELSS 197 IGG+P++TAVFVILT +LGT++GP V+R FRIDNEIARGV LGT+AHG GTSKAFELSS Sbjct: 138 MIGGMPAVTAVFVILTALLGTVIGPMVIRYFRIDNEIARGVLLGTSAHGAGTSKAFELSS 197 Query: 198 LTGTISSIAMILTALLSIGLAP 219 ++GTISS++MIL A++++ AP Sbjct: 198 VSGTISSVSMILAAIMTLWPAP 219 >PA3431 [M] COG1346 Putative effector of murein hydrolase Length = 228 Score = 149 bits (377), Expect = 2e-36, Method: Compositional matrix adjust. Identities = 74/169 (43%), Positives = 110/169 (65%) Query: 51 YESYNAGGKWLTDLLQPATIAFAIPLHKNFKVLKKHAAEIAAGVLSGTVMAVLSSMLLAK 110 Y Y WL LL PAT+AFAIP+ + ++L +H + AG+ +GT +A+ SS LA+ Sbjct: 54 YADYARDTNWLVALLGPATVAFAIPIWQQRELLMRHWPALLAGMFAGTAVAIGSSWALAQ 113 Query: 111 WLHLSGDLATSLVPRSVTTPIAMSISQSIGGVPSITAVFVILTGVLGTLMGPSVLRLFRI 170 L L G + SL+PRS+TTP AM +S +GGVP +TA FV++TGV G ++G ++LR+ R+ Sbjct: 114 ALALDGQVTLSLLPRSITTPFAMEMSHDLGGVPELTAAFVMITGVFGAVIGGTLLRVLRL 173 Query: 171 DNEIARGVSLGTAAHGTGTSKAFELSSLTGTISSIAMILTALLSIGLAP 219 +ARG G AHG GTS+A+E G+ + + M+LT L ++ +AP Sbjct: 174 RTPLARGALFGVGAHGAGTSRAYEFGGEEGSAAGLLMVLTGLFNLLVAP 222 >VC2100 [M] COG1346 Putative effector of murein hydrolase Length = 229 Score = 138 bits (348), Expect = 6e-33, Method: Compositional matrix adjust. Identities = 70/169 (41%), Positives = 117/169 (69%) Query: 51 YESYNAGGKWLTDLLQPATIAFAIPLHKNFKVLKKHAAEIAAGVLSGTVMAVLSSMLLAK 110 Y++Y A WL LL PAT+AFA+P++ N +L++H ++ GV+ ++A+ S++LLA+ Sbjct: 54 YQAYMADAHWLLWLLGPATVAFAVPVYDNRALLRQHWLSLSVGVVVSVLVAISSTVLLAR 113 Query: 111 WLHLSGDLATSLVPRSVTTPIAMSISQSIGGVPSITAVFVILTGVLGTLMGPSVLRLFRI 170 WL+L L SL RS+TTP A+ ++SIGG +TA+FV+LTGV+G +G +L + I Sbjct: 114 WLNLPDLLQRSLAMRSITTPFAVEATKSIGGQSDLTALFVVLTGVIGMAVGEIILTVLSI 173 Query: 171 DNEIARGVSLGTAAHGTGTSKAFELSSLTGTISSIAMILTALLSIGLAP 219 + + +G SLG +AHG GT+KA+++ + G +SS+ M+L ++++ +AP Sbjct: 174 RSRLGKGASLGASAHGAGTAKAYQIGNSEGVVSSVVMMLAGMVTVVIAP 222 >SA2328 [M] COG1346 Putative effector of murein hydrolase Length = 229 Score = 129 bits (325), Expect = 3e-30, Method: Compositional matrix adjust. Identities = 64/175 (36%), Positives = 107/175 (61%) Query: 51 YESYNAGGKWLTDLLQPATIAFAIPLHKNFKVLKKHAAEIAAGVLSGTVMAVLSSMLLAK 110 Y Y GG W+ +L + A PL+KN + +K + + I A VL+G ++ + L K Sbjct: 54 YNGYMKGGSWINHILNATVVCLAYPLYKNREKIKDNVSIIFASVLTGVMLNFMLVFLTLK 113 Query: 111 WLHLSGDLATSLVPRSVTTPIAMSISQSIGGVPSITAVFVILTGVLGTLMGPSVLRLFRI 170 S D+ +L+PRS+T + + +S +GG ++T +F+I TG++G+++G +LR R Sbjct: 114 AFGYSKDVIVTLLPRSITAAVGIEVSHELGGTDTMTVLFIITTGLIGSILGSMLLRFGRF 173 Query: 171 DNEIARGVSLGTAAHGTGTSKAFELSSLTGTISSIAMILTALLSIGLAPALLAVF 225 ++ IA+G++ G A+H GT+KA E+ +G SSI MILTA++S L P L+ +F Sbjct: 174 ESSIAKGLTYGNASHAFGTAKALEMDIESGAFSSIGMILTAVISSVLIPVLILLF 228 >FN0468 [M] COG1346 Putative effector of murein hydrolase Length = 230 Score = 125 bits (313), Expect = 6e-29, Method: Compositional matrix adjust. Identities = 58/170 (34%), Positives = 106/170 (62%) Query: 52 ESYNAGGKWLTDLLQPATIAFAIPLHKNFKVLKKHAAEIAAGVLSGTVMAVLSSMLLAKW 111 + Y G + LL PAT+A A+PL+K + + KK + G + G+ + ++S ++L K Sbjct: 57 DDYYKGAGMILFLLGPATVALAVPLYKKWDLFKKFFVPVMTGAVVGSFVGIISVIILGKL 116 Query: 112 LHLSGDLATSLVPRSVTTPIAMSISQSIGGVPSITAVFVILTGVLGTLMGPSVLRLFRID 171 + L SL+P+S+TTP + IS +GG+P+IT V ++LTG+ G + P + ++FR+ Sbjct: 117 FGMEEQLIFSLMPKSITTPFGIEISSMLGGIPAITVVSIMLTGITGNVTAPLISKIFRVK 176 Query: 172 NEIARGVSLGTAAHGTGTSKAFELSSLTGTISSIAMILTALLSIGLAPAL 221 + +A G+ +G ++H GTSKA E+ + G++S+++++ LL++ P L Sbjct: 177 HSVAVGIGIGVSSHAVGTSKAMEIGEIEGSMSALSIVFAGLLTLIWVPLL 226 Database: COG Posted date: Aug 3, 2010 9:58 AM Number of letters in database: 38,378,431 Number of sequences in database: 115,334 Lambda K H 0.320 0.132 0.367 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 115334 Number of Hits to DB: 12,361,166 Number of extensions: 432977 Number of successful extensions: 1326 Number of sequences better than 1.0e-05: 13 Number of HSP's gapped: 1325 Number of HSP's successfully gapped: 13 Length of query: 227 Length of database: 38,378,431 Length adjustment: 103 Effective length of query: 124 Effective length of database: 26,499,029 Effective search space: 3285879596 Effective search space used: 3285879596 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.4 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.9 bits) S2: 114 (48.5 bits)