BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0023.0005.p (322 letters) Database: COG 115,334 sequences; 38,378,431 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value PA0237 [R] COG0673 Predicted dehydrogenases and related proteins 93 7e-19 BS_yrbE [R] COG0673 Predicted dehydrogenases and related proteins 89 7e-18 SSO3049 [R] COG0673 Predicted dehydrogenases and related proteins 86 7e-17 TM0414 [R] COG0673 Predicted dehydrogenases and related proteins 84 2e-16 BH2221 [R] COG0673 Predicted dehydrogenases and related proteins 82 8e-16 >PA0237 [R] COG0673 Predicted dehydrogenases and related proteins Length = 347 Score = 92.8 bits (229), Expect = 7e-19, Method: Compositional matrix adjust. Identities = 87/350 (24%), Positives = 146/350 (41%), Gaps = 50/350 (14%) Query: 3 LNIGIVGTGWFSKVHADLLAGMEDVKLQAVCGSSKQKGEDMASPYGAEGYGDITEMLDAR 62 L I ++G G + H L + +L C + +G+ A+ G +GD +L+ Sbjct: 5 LRIALIGAGNMGRQHYHRLQRIAGARL---CAVADPQGQAFAADTGVPWFGDHRRLLEEA 61 Query: 63 KLDAVYICVPPQSHGAIERALIRREIPFFVEKPLGSSTEIPASLLQDIKEHGLLTSVGYH 122 + A + P H A + +P +EKP+G + + L+ + G+ VG+H Sbjct: 62 RPQAAIVANPNNLHVATALDCLAAGVPLLLEKPVGVQLDEVSELVAVARRSGVPLLVGHH 121 Query: 123 FRYQENIARLKKLLSGDKVGMIV---GQWMGSMPG----VAWWRDQEQSGGQFTEQTTHI 175 R+ + R ++L++ +G ++ W P VAW R E G H Sbjct: 122 RRHNPLVVRARQLIAEGALGRLLSVTALWQLKKPDSYFEVAWRR--EPGAGMLLTNLIHD 179 Query: 176 VDLLRYLGGEVTEVYGMFGNRIVHEQHEGVTVADVGTVSLKLDNGIVANIS--------- 226 +DLLR+L GEV EV + GN I G+ D + L+ NG + +++ Sbjct: 180 LDLLRHLCGEVREVQALAGNAI-----RGLPNEDNIALLLRFANGALGSLTGCDAAAAPW 234 Query: 227 -------NTCVLPGEVGQVGLSFYNDKGLLD------WNPDRLMEVRSG------DSKEY 267 V P + Q +G L W R E R G S+E Sbjct: 235 SWELAAGENPVYPRQAEQPCYLLAGTEGALSLPQLRRW---RYAEARQGWHDPLAASEEA 291 Query: 268 VSSGNPYAAESEAFLHAVRTGDRSRILSDYEDGYKTLKVTCAAYESAKSG 317 V++G+P + E F+ R G+ + ++ D D +TL + A E+A+SG Sbjct: 292 VATGDPLQRQLEHFVRVAR-GEEAPLM-DATDAARTLALVEAVREAARSG 339 >BS_yrbE [R] COG0673 Predicted dehydrogenases and related proteins Length = 260 Score = 89.4 bits (220), Expect = 7e-18, Method: Compositional matrix adjust. Identities = 0/340 (0%), Positives = 0/340 (0%), Gaps = 25/340 (7%) Query: 1 MTLNI--GIVGTGWFSKVHADLLAGMEDVKLQAVCGSSKQKGEDMASPYGAEGY-GDITE 57 M LN + G + M L K + Y I Sbjct: 1 MLLNALASLGHKGIKTLRTFGRAGLMLFNALVGKPEFRKHAPLLVRQLYNVGVLSMLIIV 60 Query: 58 MLDARKLDAVYICVPPQSHGAIERALIRREIPFFVEKPLGSSTEIPASLLQDIKEHGLLT 117 + + + + + + + LG + Sbjct: 61 VSGVFIGMVLGLQGYLVLTTYSAETSLGMLVALSLLRELGPVVAALLFAGRAGSALTAEI 120 Query: 118 SVGYHFRYQENIARLKKLLSGDKVG----MIVGQWMGSMPGVAWWRDQEQSGGQFTEQTT 173 + ++ + + V GG + Sbjct: 121 GLMRATEQLSSMEMMAVDPLRRVISPRFWAGVISLPLLTVIFVAVG---IWGGSLVGVSW 177 Query: 174 HIVDLLRY-LGGEVTEVYGMFGNRIVHEQHEGVTVADVGTVSLKLDNGIVANISNT-CVL 231 +D + + + M + + + I + + Sbjct: 178 KGIDSGFFWSAMQNAVDWRM-DLVNCLIKSVVFAITVTWISLFNGYDAIPTSAGISRATT 236 Query: 232 PGEVGQVGLSFYNDKGLLDWNPDRLMEVRSGDSKEYVSSGNPY---------AAESEAFL 282 Sbjct: 237 --- 293 Query: 283 HAVRTGDRSRILSDYEDGYKTLKVTCAAYESAKSGLPVKL 322 Sbjct: 294 333 >SSO3049 [R] COG0673 Predicted dehydrogenases and related proteins Length = 371 Score = 85.9 bits (211), Expect = 7e-17, Method: Compositional matrix adjust. Identities = 94/348 (27%), Positives = 136/348 (39%), Gaps = 32/348 (9%) Query: 3 LNIGIVGTGWFSKVHADLLAGMED----VKLQAVCGSSKQKGEDMASPYGAEGYGDITEM 58 + + +VG G K H L +E VKL AV K E + S YG Y I E+ Sbjct: 20 IGVAVVGLGSIGKTHVKALKDLEKETEFVKLVAVVDQIKAIAEKIGSEYGTPYYTTIDEV 79 Query: 59 LDARKLDAVYICVPPQSHGAIERALIRREIPFFVEKPLGSSTEIPASLLQDIKEHGLLTS 118 L ++D + I P H I VEKP+ ++ ++ + + + Sbjct: 80 LRNSEVDVISIATPSYLHAPQAILAIEYGKHVIVEKPMATTLAGAREMVSRAERNEVKLG 139 Query: 119 VGYHFRYQENIARLKK--LLSGDKVGMIVGQ--WMGSMPG-------VAWWRD--QEQSG 165 V + RY +I RLK L ++ +I + W M G WR + G Sbjct: 140 VIFQERYAPDIRRLKNDILKELGRIYLIESELKWYRDMKGYYKRDEIARSWRGMWNTEGG 199 Query: 166 GQFTEQTTHIVDLLRYLGGEVTEVYGMFGNRIVHEQHEGVTVADVGTVSLKLDNGIVANI 225 G T Q H +DL+ +L GEV EV G N H+G+ V D ++ NG + I Sbjct: 200 GVMTNQGIHTIDLMIWLNGEVEEVSGFVDNL----THDGIEVEDTAVAIMRYKNGALGTI 255 Query: 226 SNTCVL-PGEVGQVGLSFYNDKGLLDWNPDRLMEVRSGDSKEYVSSGNPYAAESEA---F 281 S T + P + G ++ L V E S Y E+ A Sbjct: 256 SQTVSMKPTTYQYRKIRVNGSNGFVEITDGSLSTVAIEGKIEESKSSVEYKKETIAQPGN 315 Query: 282 LHAVRTGDRSRILSDYED-------GYKTLKVTCAAYESAKSGLPVKL 322 LH D R LS+ D G K+L+V A Y S+ + VKL Sbjct: 316 LHKELLRDFLRALSEDNDFPINGKEGMKSLEVIKAVYLSSVNRQVVKL 363 >TM0414 [R] COG0673 Predicted dehydrogenases and related proteins Length = 334 Score = 84.3 bits (207), Expect = 2e-16, Method: Compositional matrix adjust. Identities = 57/231 (24%), Positives = 107/231 (46%), Gaps = 8/231 (3%) Query: 3 LNIGIVGTGWFSKVHADLLAGMEDVKLQAVCGSSKQKGEDMASPYGAE-GYGDITEMLDA 61 + IG++G G +HA+ L ++D L A+ + + +M G E Y D E+++ Sbjct: 1 MRIGVIGLGRIGTIHAENLKMIDDAILYAISDVREDRLREMKEKLGVEKAYKDPHELIED 60 Query: 62 RKLDAVYICVPPQSHGAIERALIRREIPFFVEKPLGSSTEIPASLLQDIKEHGLLTSVGY 121 +DAV +C +H + A + + F EKPL + ++++ K+ ++ G+ Sbjct: 61 PNVDAVLVCSSTNTHSELVIACAKAKKHVFCEKPLSLNLADVDRMIEETKKADVILFTGF 120 Query: 122 HFRYQENIARLKKLLSGDKVG----MIVGQWMGSMPGVAWWRDQEQSGGQFTEQTTHIVD 177 + R+ N +LK+ + +G + + + P + + R SGG F + T H D Sbjct: 121 NRRFDRNFKKLKEAVENGTIGKPHVLRITSRDPAPPPLDYIR---VSGGIFLDMTIHDFD 177 Query: 178 LLRYLGGEVTEVYGMFGNRIVHEQHEGVTVADVGTVSLKLDNGIVANISNT 228 + RY+ GE E G+ +V E+ D V L+ +G + I N+ Sbjct: 178 MARYIMGEEVEEVFADGSVLVDEEIGKAGDVDTAVVVLRFKSGALGVIDNS 228 >BH2221 [R] COG0673 Predicted dehydrogenases and related proteins Length = 345 Score = 82.4 bits (202), Expect = 8e-16, Method: Compositional matrix adjust. Identities = 65/240 (27%), Positives = 113/240 (47%), Gaps = 24/240 (10%) Query: 2 TLNIGIVGTGWFSKVHAD--------LLAGMEDVKLQAVCGSSKQKGEDMASPYGAEGYG 53 T+NIG++G G ++HA L + DV+L + G+ ++ ++ + Sbjct: 3 TVNIGLIGAGRIGQLHASNIISSPYMELKAVSDVQLDHLKGTEIERSVEIMTT------- 55 Query: 54 DITEMLDARKLDAVYICVPPQSHGAIERALIRREIPFFVEKPLGSSTEIPASLLQDIKEH 113 + E+ R +DA++IC +H A +A F EKP+ L + E Sbjct: 56 NPDELFSNRDIDAIFICSSTDTHAAFIKAAALAGKHIFCEKPISFHVTETKGALNIVNEA 115 Query: 114 GLLTSVGYHFRYQENIARLKKLLSGDKVG----MIVGQWMGSMPGVAWWRDQEQSGGQFT 169 G+ VG++ RY ++ R+++ + +G + V P +++ E+SGG F Sbjct: 116 GVKFQVGFNRRYDKHFRRVQECVQAGAIGAPHIIRVSSRDPEAPPLSYV---ERSGGMFM 172 Query: 170 EQTTHIVDLLRYL-GGEVTEVYGMFGNRIVHEQHEGVTVADVGTVSLKLDNGIVANISNT 228 + T H D++RYL G EVTEV N +V + V D ++L L+NG +A I N+ Sbjct: 173 DMTIHDFDMVRYLSGSEVTEVSVKAAN-LVDSRIGEVGDVDTAIITLMLENGAIAVIDNS 231 Database: COG Posted date: Aug 3, 2010 9:58 AM Number of letters in database: 38,378,431 Number of sequences in database: 115,334 Lambda K H 0.317 0.136 0.403 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 115334 Number of Hits to DB: 25,591,522 Number of extensions: 1092584 Number of successful extensions: 2199 Number of sequences better than 1.0e-05: 76 Number of HSP's gapped: 2158 Number of HSP's successfully gapped: 76 Length of query: 322 Length of database: 38,378,431 Length adjustment: 106 Effective length of query: 216 Effective length of database: 26,153,027 Effective search space: 5649053832 Effective search space used: 5649053832 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.3 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.6 bits) S2: 116 (49.3 bits)