BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0022.0001.p (257 letters) Database: COG 115,334 sequences; 38,378,431 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value lin2265 [G] COG1082 Sugar phosphate isomerases/epimerases 159 3e-39 BH3346 [G] COG1082 Sugar phosphate isomerases/epimerases 119 4e-27 CC1629 [G] COG1082 Sugar phosphate isomerases/epimerases 112 7e-25 AGl2948 [G] COG1082 Sugar phosphate isomerases/epimerases 96 5e-20 mll3313 [G] COG1082 Sugar phosphate isomerases/epimerases 89 5e-18 >lin2265 [G] COG1082 Sugar phosphate isomerases/epimerases Length = 301 Score = 159 bits (403), Expect = 3e-39, Method: Compositional matrix adjust. Identities = 10/234 (4%), Positives = 35/234 (14%), Gaps = 11/234 (4%) Query: 4 IKQQIGIQMYTLRDRAEKDFLGTLRKVAEIGYKSVEFAGYFGVSAAELRKTLDELGLSAP 63 + + TL S+ + + + Sbjct: 1 MSIDKSYCGFIAIVGRPNVGKSTLLNKLLGQKISITSRKAQTTRHRIVGIHTEGAYQAIY 60 Query: 64 SAHVGLDFSSLENMQSALSREIEYAREVGLEYLITPSAPLPEQPALDDVLALIPFFEKAS 123 L + + + + E ++ + Sbjct: 61 VDTP-----GLHMEEKRAINRLMNKAASSSIGDVELVIFVVEGTRWTPDDEMVLNKLRE- 114 Query: 124 ELVRAAGLKYGYHNHDFEFKQVNGQAVIDIWLERIPAEHMVAEFDLGWVHVGGAVPAEYI 183 +A + + + K + + + + + A Sbjct: 115 --GKAPVILAVNKVDNVQEKADLLPHLQFLASQMNF--LDIVPISAETGLNVDTIAAIVR 170 Query: 184 SRYAGRVPLVHIKDFGAGHEEAD-LGKGQVDFDSVFKAADEAGIQHYIVEQEVF 236 + + A + I Sbjct: 171 KHLPEATHHFPEDYITDRSQRFMASEIIREKLMRFLGAELPYSVTVEIERFVSN 224 >BH3346 [G] COG1082 Sugar phosphate isomerases/epimerases Length = 241 Score = 119 bits (299), Expect = 4e-27, Method: Compositional matrix adjust. Identities = 76/234 (32%), Positives = 129/234 (55%), Gaps = 17/234 (7%) Query: 4 IKQQIGIQMYTLRDRAEKDFLGTLRKVAEIGYKSVEFAGYFGVSAAE-LRKTLDELGLSA 62 +K QI +Q+YT+R E+DF GTLRKVA IG++ VE A +G + E L+ L +GL Sbjct: 1 MKNQIALQLYTVRKPLEEDFEGTLRKVASIGFQGVELADDWGGKSPEALKAILQSVGLVP 60 Query: 63 PSAHVGLDFSSLENMQSALSREIEYAREVGLEYLITPSAPLPEQPALDDVLALIPFFEKA 122 S+HV +LE +++ L I+ +G + P Q A LI ++ Sbjct: 61 VSSHV-----ALERLENQLDDVIDQHLRLGCHTIYCPYLEEERQTAT-GYKELITSLKEI 114 Query: 123 SELVRAAGLKYGYHNHDFEFKQVNGQAVIDIWLERIPAEHMVAEFDLGWVHVGGAVPAEY 182 + + G+ +GYH+HDFE +A++ IP+ ++AE D+ W+ G P E+ Sbjct: 115 QKKLEKKGISFGYHHHDFELYSNQLEAILS-----IPS--LLAELDVYWLQKAGQDPVEW 167 Query: 183 ISRYAGRVPLVHIKDFGAGHEE--ADLGKGQVDFDSVFKAADEAGIQHYIVEQE 234 +SRY +V VH+KD +E A +G G+++ D++ +++ + ++ +IVEQ+ Sbjct: 168 VSRYKHKVRHVHLKDMTNDDQEFFAPVGTGKINIDAILESST-SFVEWWIVEQD 220 >CC1629 [G] COG1082 Sugar phosphate isomerases/epimerases Length = 328 Score = 112 bits (279), Expect = 7e-25, Method: Compositional matrix adjust. Identities = 78/255 (30%), Positives = 117/255 (45%), Gaps = 27/255 (10%) Query: 5 KQQIGIQMYTLRDRAEKDFLGTLRKVAEIGYKSVEFAGYFGVSAAELRKTLDELGLSAPS 64 Q +GIQ+Y+L K+ L VA+IG+++VE AGY G + AELR D GL PS Sbjct: 53 NQPVGIQLYSLGPDLAKELDAQLATVAKIGFRTVELAGYLGRTPAELRALFDRHGLVCPS 112 Query: 65 AHVGLDFSSLENMQSALSREIEYAREVGLEYLITPSAPLPE------------QPALDDV 112 AH+ L + + +G + I P +PE Q D+ Sbjct: 113 AHISP--KGPNGFSGDLVKLADELHVIGAKSAIMPIFYIPERMGAADLRQAGVQMTADEW 170 Query: 113 LALIPFFEKASELVRAAGLKYGYHNHDFEF---KQVNGQAV--IDIWLERIPAEHMVAEF 167 F + + +++ AG+ GYHNH+FEF K G+ +DI L+ + E Sbjct: 171 KWNADFLNEKAAILKKAGILAGYHNHNFEFAPLKDAKGRETTGMDILLKGTDPSLVTFEL 230 Query: 168 DLGWVHVGGAVPAEYISRYAGRVPLVHIKD--------FGAGHEEADLGKGQVDFDSVFK 219 D GWV G P + Y GR +H+KD F + ++G G +D+ + Sbjct: 231 DAGWVTAAGQDPFALLKAYPGRFTQMHVKDVKPSTKPNFELRQDPTEVGSGMIDWKRLLP 290 Query: 220 AADEAGIQHYIVEQE 234 AA +AGI+ + EQE Sbjct: 291 AAYDAGIRGFYYEQE 305 >AGl2948 [G] COG1082 Sugar phosphate isomerases/epimerases Length = 353 Score = 96.3 bits (238), Expect = 5e-20, Method: Compositional matrix adjust. Identities = 10/256 (3%), Positives = 35/256 (13%), Gaps = 39/256 (15%) Query: 1 MAAIKQQIGIQMYTLRDRAEKDFLGTLRKVAEIGYKSVEFAGYFGVSAAE-----LRKTL 55 M + + + V G + A Sbjct: 1 MKYKTLGNTGLLVSQLCL--GTMTFSDGTGIYEHIGDVGQTGADELVKASIDAGINFFDT 58 Query: 56 DELGLSAPSAHVGLDFSSLENMQSALSREIEYAREVGLEYLITPSAPLPEQPALDDVLAL 115 ++ + S ++ E + + + Sbjct: 59 ADVYSAGASERTLGQS-----FKNLGIARNEIVLATKVYSRMGSGRNDVGASR--GHIMD 111 Query: 116 IPF---FEKASELVRAAGLKYGYHNHDFEFKQVNGQAVIDIWLERIPAEHMVA------- 165 ++ + + E + + V Sbjct: 112 AVEASLKRLQTDHIDLYQIHATDTLTPVEETLRALDDL--------VRQGKVRYVGVSNW 163 Query: 166 EFDLGWVHVGGAVPAEYISRYAGRVPLVHIKDFGAGHEE------ADLGKGQVDFDSVFK 219 + +G + + + Sbjct: 164 QAWRIATALGVSARLGLERFNTLQAYYSIAGRDLERELKPLLDAEKMGLLVWSPLAGGLL 223 Query: 220 AAD-EAGIQHYIVEQE 234 + Q + Sbjct: 224 SGRFSRANQSPEGSRR 239 >mll3313 [G] COG1082 Sugar phosphate isomerases/epimerases Length = 301 Score = 89.4 bits (220), Expect = 5e-18, Method: Compositional matrix adjust. Identities = 9/247 (3%), Positives = 26/247 (10%), Gaps = 46/247 (18%) Query: 11 QMYTLRDRAEKDFLGTLRKVAEIGYKSVEFAGYFGV--SAAELRKTLDELGLSAPSAHVG 68 + + + + + A A Sbjct: 6 SYCGFIAI--VGRPNVGKSTLLNKLLGQK--ISITSRKAQTTRHRIVGIHTEGAYQAIYV 61 Query: 69 LDFSSLENMQSALSREIEYARE----VGLEYLITPSAPLPEQPALDDVLALIPFFE---K 121 E L + P E Sbjct: 62 DT---------PGLHMEEKRAINRLMNKAASSSIGDVELVIFVV--EGTRWTPDDEMVLN 110 Query: 122 ASELVRAAGLKYGYHNHDFEFKQVNGQAVIDIWLERIPAEHMVA-------EFDLGWVHV 174 +A + + + K + ++V Sbjct: 111 KLREGKAPVILAVNKVDNVQEKADLLPHL--------QFLASQMNFLDIVPISAETGLNV 162 Query: 175 GGAVPAEYISRYAGRVPLVHIKDF---GAGHEE---ADLGKGQVDFDSVFKAAD-EAGIQ 227 + + + + Sbjct: 163 DTIAAIVRKHLPEATHHFPEDYITDRSQRFMASEIIREKLMRFLGAELPYSVTVEIERFV 222 Query: 228 HYIVEQE 234 Sbjct: 223 SNERGGY 229 Database: COG Posted date: Aug 3, 2010 9:58 AM Number of letters in database: 38,378,431 Number of sequences in database: 115,334 Lambda K H 0.320 0.138 0.402 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 115334 Number of Hits to DB: 19,107,186 Number of extensions: 875297 Number of successful extensions: 1693 Number of sequences better than 1.0e-05: 5 Number of HSP's gapped: 1679 Number of HSP's successfully gapped: 5 Length of query: 257 Length of database: 38,378,431 Length adjustment: 104 Effective length of query: 153 Effective length of database: 26,383,695 Effective search space: 4036705335 Effective search space used: 4036705335 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.4 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.8 bits) S2: 114 (48.5 bits)