BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0021.0014.p (364 letters) Database: COG 115,334 sequences; 38,378,431 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value SA0150 [M] COG0381 UDP-N-acetylglucosamine 2-epimerase 326 3e-89 SP0360 [M] COG0381 UDP-N-acetylglucosamine 2-epimerase 270 2e-72 STM0610 [R] COG3381 Uncharacterized component of anaerobic dehyd... 160 4e-39 TM0610 [M] COG0381 UDP-N-acetylglucosamine 2-epimerase 160 4e-39 SA1913 [M] COG0381 UDP-N-acetylglucosamine 2-epimerase 147 4e-35 >SA0150 [M] COG0381 UDP-N-acetylglucosamine 2-epimerase Length = 374 Score = 326 bits (836), Expect = 3e-89, Method: Compositional matrix adjust. Identities = 160/360 (44%), Positives = 242/360 (67%), Gaps = 7/360 (1%) Query: 1 MKIMTILGTRPEIIRLSVIIPLLDEHAEQHVLVHTGQNFTASLSGIFFAELGLRAPDYVL 60 +K+MTI+GTRPEIIRLS I D++ Q +LVHTGQN+ +L+ IFF +L LR PD+ L Sbjct: 4 LKLMTIVGTRPEIIRLSSTIKACDQYFNQ-ILVHTGQNYDYTLNQIFFDDLELRQPDHYL 62 Query: 61 QDKQAGLGGQLAAMFGSLESILLQEQPDRILLLGDTNSALCAILAERMGIPVVHMEAGNR 120 + + LG + + +LL+EQPD +L+LGDTNS L A+ A+R+ IPV HMEAGNR Sbjct: 63 EAVGSNLGETMGNIIAKTYDVLLREQPDALLILGDTNSCLAAVSAKRLKIPVFHMEAGNR 122 Query: 121 CYDLKVPEEKNRRVIDAVSTINMPYTQQSKRHLLSEGFPSQRIVLTGNPIHEVIKHYNRQ 180 C+D VPEE NR+++D VS +N+PYT+ S+R+LL EGF I +TG+P+ EVI+ + + Sbjct: 123 CFDQNVPEEINRKIVDHVSDVNLPYTEHSRRYLLDEGFNKANIFVTGSPMTEVIEAHRDK 182 Query: 181 IGASDILDRLNLMAGQYFLVTAHRAENVDDPESLRQIMSGLNLVAEHFGLRLICSIHPRT 240 I SD+L++L L QY LV+AHR EN+D+ ++ + +M+ +N +A+ + + +I S HPR+ Sbjct: 183 INHSDVLNKLGLEPQQYILVSAHREENIDNEKNFKSLMNAINDIAKKYKMPVIYSTHPRS 242 Query: 241 RSKLAEQFKLEMNPLVEFHEPFGFFDFVHLEQYALCAITDSGTVQEECCLMGVPTVTIRR 300 K+ E+ K E +PLV+ +PFGFFD+ L++ A ++DSGT+ EE ++ P V IR Sbjct: 243 WKKI-EESKFEFDPLVKQLKPFGFFDYNALQKDAFVVLSDSGTLSEESSILKFPGVLIRT 301 Query: 301 TTERPETVDCGSNVVSGVEQESILRCTRLMTSL----SPEWEAPEGYLTPDVSVKVVKFL 356 +TERPE +D G+ +V G+ ++++ L + P +A + Y +VS KVVK + Sbjct: 302 STERPEVLDKGTVIVGGITYNNLIQSVELAREMQNNNEPMIDAID-YKDTNVSTKVVKII 360 >SP0360 [M] COG0381 UDP-N-acetylglucosamine 2-epimerase Length = 394 Score = 270 bits (691), Expect = 2e-72, Method: Compositional matrix adjust. Identities = 142/366 (38%), Positives = 225/366 (61%), Gaps = 14/366 (3%) Query: 1 MKIMTILGTRPEIIRLSVIIPLLDEHAEQHVLVHTGQNFTASLSGIFFAELGLRAPDYVL 60 +K++ I+GTRPEIIRLS +I ++ + +L HTGQN+ +L+GIFF LGL PD + Sbjct: 18 LKLLIIVGTRPEIIRLSSVITKCRKYFDV-ILAHTGQNYDYNLNGIFFDNLGLDTPDVYM 76 Query: 61 QDKQAGLGGQLAAMFGSLESILLQEQPDRILLLGDTNSALCAILAERMGIPVVHMEAGNR 120 LG + + + ++ Q +PD +L+LGDTNS L AI A+R+ IP+ HMEAGNR Sbjct: 77 DAVGDDLGATVGNIINTSYKLMNQIKPDALLILGDTNSCLSAIAAKRLHIPIFHMEAGNR 136 Query: 121 CYDLKVPEEKNRRVIDAVSTINMPYTQQSKRHLLSEGFPSQRIVLTGNPIHEVIKHYNRQ 180 C D +PEE NRR++D +S +N+ Y++ ++++L G P +R +TG+P+ EV+ Sbjct: 137 CKDECLPEETNRRIVDVISDVNLAYSEHARKYLHECGLPKERTYVTGSPMAEVLHKNLSA 196 Query: 181 IGASDILDRLNLMAGQYFLVTAHRAENVDDPESLRQIMSGLNLVAEHFGLRLICSIHPRT 240 I +SDI +RL L G Y L++AHR EN+D ++ + + +N +AE + + ++ S HPR+ Sbjct: 197 IESSDIHERLGLKKGGYILLSAHREENIDTDKNFISLFTAINQLAEKYNMPILYSCHPRS 256 Query: 241 RSKLAEQ-FKLEMNPLVEFHEPFGFFDFVHLEQYALCAITDSGTVQEECCLMG-----VP 294 + +L E FKL+ V HEP GF D+ L+ A ++DSGT+ EE P Sbjct: 257 KKRLQESGFKLDKR--VIQHEPLGFHDYNCLQMNAFVVVSDSGTLPEESSFFTSQGYPFP 314 Query: 295 TVTIRRTTERPETVDCGSNVVSGVEQESILRCTRLMTSLSPEWE----APEGYLTPDVSV 350 V IR +TERPE++D +++G+++ S+L+ SL+ + + P+ Y+ +VS Sbjct: 315 AVCIRTSTERPESLDKAGFILAGIDENSLLQAVETAVSLAQDEDFGLPVPD-YVEENVST 373 Query: 351 KVVKFL 356 KVVK + Sbjct: 374 KVVKII 379 >STM0610 [R] COG3381 Uncharacterized component of anaerobic dehydrogenases Length = 289 Score = 160 bits (404), Expect = 4e-39, Method: Compositional matrix adjust. Identities = 0/323 (0%), Positives = 0/323 (0%), Gaps = 9/323 (2%) Query: 1 MKIMTILGTRPEIIRLSVIIPLLDEHAEQHVLVHTGQNFTASLSGIFFAELGLRAPDYVL 60 + L + L + G A L L A + Sbjct: 16 GHLDQPLSLDNVAAKAGYSKWHLQRMFKDVTGHAIGAYIRARRLSKSAVALRLTARPILD 75 Query: 61 QDKQAGLGGQLAAMFGSLESILLQEQPDRILLLGDTNSALC-AILAERMGIPVVHMEAGN 119 Q Q + R L E + + Sbjct: 76 IALQYRFDSQQTFTRAFKKQFAQTPALYRRSPEWSAFGIRPPLRLGEFTMPEHKFVTLED 135 Query: 120 RCYDLKVPEEKNRRVIDAVSTINMPYTQQSKRHLLSEGFPSQRIVLTGNPIHEVIKHYNR 179 + M Y + + K + Sbjct: 136 TPLIGVTQSYSCSLEQISDFRHEMRYQFWHDFLGNAPTI-PPVLYGLNETRPSQDKDDEQ 194 Query: 180 QIGASDILDRLNLMAGQYFLVTAHRAENVDDPESLRQIMSGLNLVAEHFGLRLICSIHPR 239 ++ + G + + + + + Sbjct: 195 EV-FYTTALAQDQADGYVLTGHPVMLQGGEYVMFTYEGLGTGVQEFIL--TVYGTCMPML 251 Query: 240 TRSKLAEQFKLE--MNPLVEFHEPFGFFDFVHLEQYALCAITDSGTVQEECCLMGVPTVT 297 ++ +E Sbjct: 252 NLTRRKG-QDIERYYPAEDAK- 309 Query: 298 IRRTTERPETVDCGSNVVSGVEQ 320 Sbjct: 310 332 >TM0610 [M] COG0381 UDP-N-acetylglucosamine 2-epimerase Length = 374 Score = 160 bits (404), Expect = 4e-39, Method: Compositional matrix adjust. Identities = 99/323 (30%), Positives = 177/323 (54%), Gaps = 9/323 (2%) Query: 1 MKIMTILGTRPEIIRLSVIIPLLDEHAEQHVLVHTGQNFTASLSGIFFAELGLRAPDYVL 60 +++++++G RP+II+ +++ E + +LVH+GQ++ ++S +FF L +R P Y L Sbjct: 16 LRVLSLVGARPQIIKEAMLHREFKEKGIEEILVHSGQHYDYNMSSVFFEILEIRQPHYNL 75 Query: 61 QDKQAGLGGQLAAMFGSLESILLQEQPDRILLLGDTNSALC-AILAERMGIPVVHMEAGN 119 G + E +LL E+PD +L+ GDTN+ L A++A ++ IPV H+EAG Sbjct: 76 NVGSGTHGEMTGKIMIEFEKVLLSEKPDLVLVYGDTNTTLSGALVAAKLKIPVAHVEAGL 135 Query: 120 RCYDLKVPEEKNRRVIDAVSTINMPYTQQSKRHLLSEGFPSQRIVLTGNPIHEVIKHYNR 179 R + +PEE NR V D VS + ++ + R+L EG ++ + G+ ++++ Sbjct: 136 RQHPKDMPEEINRIVTDRVSQLLFCPSKLAVRNLEREGI-TEGVYFVGDVMYDLFLKMED 194 Query: 180 QIGASDILDRLNLMAGQYFLVTAHRAENVDDPESLRQIMSGLNLVAEHFGLRLICSIHPR 239 + D+ ++L L ++ LVT HR NVDDP LR+I+ L V+ +++ IHPR Sbjct: 195 RF-RYDVFEKLGLKENEFVLVTLHRDFNVDDPVKLRKILEQLKRVSRE--KKVVFPIHPR 251 Query: 240 TRSKLAEQFKLE--MNPLVEFHEPFGFFDFVHLEQYALCAITDSGTVQEECCLMGVPTVT 297 T++++ E F LE + +V +P + + + L + +TDSG +Q+E G + Sbjct: 252 TKNRVKE-FGLENLLEGMVVI-DPVDYLNLMGLVKRCWKVVTDSGGLQKEAYFAGKRAIV 309 Query: 298 IRRTTERPETVDCGSNVVSGVEQ 320 + T E V+ G N ++ E Sbjct: 310 VMPDTGWRELVEAGWNKLASEEN 332 >SA1913 [M] COG0381 UDP-N-acetylglucosamine 2-epimerase Length = 376 Score = 147 bits (370), Expect = 4e-35, Method: Compositional matrix adjust. Identities = 102/334 (30%), Positives = 174/334 (52%), Gaps = 14/334 (4%) Query: 2 KIMTILGTRPEIIRLSVIIPLLD-EHAEQHVLVHTGQNFTASLSGIFFAELGLRAPDYVL 60 KIMTI GTRPE I+++ ++ L+ E + ++V T Q+ S + E+ P Y L Sbjct: 4 KIMTIFGTRPEAIKMAPLVKALEQEKMLEPIVVVTAQHREMLDSVLSTFEI---KPKYDL 60 Query: 61 QDKQAG--LGGQLAAMFGSLESILLQEQPDRILLLGDTNSALCAILAERMG-IPVVHMEA 117 ++G L + LE ++ E+PD +L+ GDT + LA +P+ H+EA Sbjct: 61 NIMKSGQTLSEITSKSITQLEQVIQLEKPDMVLVHGDTMTTFAGGLAAFYNQVPIGHVEA 120 Query: 118 GNRCYDL--KVPEEKNRRVIDAVSTINMPYTQQSKRHLLSEGFPSQRIVLTGNPIHEVIK 175 G R YD PEE NR+++ ++ ++ T+ + HLLSEG S+ +V+TGN + +K Sbjct: 121 GLRSYDKYSPFPEEVNRQLVGVLADLHFAPTKNAASHLLSEGKYSESVVVTGNTAIDAMK 180 Query: 176 HYNRQIGASDILDRLNLMAGQYFLVTAHRAENVDDPESLRQIMSGLN-LVAEHFGLRLIC 234 + S+I+D+ + ++ L+TAHR EN+ P + I + L+ E+ L L+ Sbjct: 181 YTVDDNYKSNIMDKYH--DKKFILMTAHRRENIGKP--MENIFKAVRRLIDEYTDLALVY 236 Query: 235 SIHPRTRSKLAEQFKLEMNPLVEFHEPFGFFDFVHLEQYALCAITDSGTVQEECCLMGVP 294 +H + + Q L + +E EP DF + + + +TDSG +QEE P Sbjct: 237 PMHKNPKVREVAQKILGSHDRIELIEPLDVVDFHNFAKKSYFILTDSGGIQEEAPSFNKP 296 Query: 295 TVTIRRTTERPETVDCGSNVVSGVEQESILRCTR 328 + +R TERPE V+ G+ V G ++++ + + Sbjct: 297 VLVLRSVTERPEGVEAGTLKVIGTNKQNVYQAAK 330 Database: COG Posted date: Aug 3, 2010 9:58 AM Number of letters in database: 38,378,431 Number of sequences in database: 115,334 Lambda K H 0.322 0.138 0.404 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 115334 Number of Hits to DB: 26,793,734 Number of extensions: 1072946 Number of successful extensions: 1899 Number of sequences better than 1.0e-05: 12 Number of HSP's gapped: 1851 Number of HSP's successfully gapped: 12 Length of query: 364 Length of database: 38,378,431 Length adjustment: 108 Effective length of query: 256 Effective length of database: 25,922,359 Effective search space: 6636123904 Effective search space used: 6636123904 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.4 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.9 bits) S2: 116 (49.3 bits)