BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0021.0013.p (328 letters) Database: COG 115,334 sequences; 38,378,431 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value FN1689 [MG] COG1086 Predicted nucleoside-diphosphate sugar epime... 241 1e-63 Cj1293 [MG] COG1086 Predicted nucleoside-diphosphate sugar epime... 234 1e-61 PM1007 [MG] COG1086 Predicted nucleoside-diphosphate sugar epime... 230 2e-60 SA0148 [MG] COG1086 Predicted nucleoside-diphosphate sugar epime... 220 2e-57 CC0233 [MG] COG1086 Predicted nucleoside-diphosphate sugar epime... 220 2e-57 >FN1689 [MG] COG1086 Predicted nucleoside-diphosphate sugar epimerases Length = 336 Score = 241 bits (615), Expect = 1e-63, Method: Compositional matrix adjust. Identities = 136/299 (45%), Positives = 185/299 (61%), Gaps = 19/299 (6%) Query: 1 MFNNKRILVTGGTGSWGHELIRQLLPQ-NPKEIVIFSRSESAQVAMSREY-------EDS 52 M NN IL+TGGTGS+G+ I ++L + NPK+I+I+SR E Q M + + + Sbjct: 1 MLNNSTILITGGTGSFGNRFIERILSEYNPKKIIIYSRDEFKQDLMKKNFVMKYGVEKTK 60 Query: 53 RLSFIIGDIRDKDALVAACRGIDYVFHLAALKHVPVCEDQPYEALKTNVVGTQNVIEAAI 112 +L F IGD+RDK+ L A G+DYV H AA+K VP CE P+EA+KTN+ G +NVIEAAI Sbjct: 61 KLRFFIGDVRDKERLYRAFDGVDYVIHAAAMKQVPACEYNPFEAIKTNIHGAENVIEAAI 120 Query: 113 ACNVEKAIYISTDKAANPSNFYGMTKAIGEKLFVYANLLG--SNTRFVTVRGGNVLGTNG 170 V+K + +STDKA NP N YG TK + +KLF+ AN T F VR GNV G+ G Sbjct: 121 DRGVKKVVALSTDKAVNPINLYGGTKLVSDKLFISANAYSGEKGTIFSVVRYGNVAGSRG 180 Query: 171 SVVHLFMKQIKDKGQVR--ITDMKMTRFFFTLRDAITLLFKASEVSLGGEIFVMTMPTCR 228 SV+ F KQ+ +G+ ITD+ MTRF+ L DA+ L+ KA + S GGE FV P+ Sbjct: 181 SVIPFF-KQLLSQGKTELPITDIHMTRFWMVLDDAVNLVLKALKESKGGETFVFKNPSFL 239 Query: 229 IVDLAEVLIEASGRRDVSIIEAGIRPGEKIHEILMSDFESQTTVVYDEQYLVILPTLDM 287 I +LA+ L + I E GIR GEKIHE++++ +++ T Y E Y VI P + Sbjct: 240 ITELAKAL-----NPNGIIKEVGIREGEKIHEVMITRDDAKYTYDY-EDYYVIYPNFEW 292 >Cj1293 [MG] COG1086 Predicted nucleoside-diphosphate sugar epimerases Length = 334 Score = 234 bits (598), Expect = 1e-61, Method: Compositional matrix adjust. Identities = 128/289 (44%), Positives = 174/289 (60%), Gaps = 10/289 (3%) Query: 1 MFNNKRILVTGGTGSWGHELIRQLLPQ-NPKEIVIFSRSESAQVAMSREYEDSRLSFIIG 59 MFN K IL+TGGTGS+G + LL P +I+I+SR E Q M+ + + + IG Sbjct: 1 MFNKKNILITGGTGSFGKTYTKVLLENYKPNKIIIYSRDELKQFEMASVFNAPCMRYFIG 60 Query: 60 DIRDKDALVAACRGIDYVFHLAALKHVPVCEDQPYEALKTNVVGTQNVIEAAIACNVEKA 119 D+RDK+ L AA R +D+V H AA+KHVP+ E P E +KTN+ G QNVI+A V+K Sbjct: 61 DVRDKERLSAAMRDVDFVIHAAAMKHVPIAEYNPMECIKTNIHGAQNVIDACFENGVKKC 120 Query: 120 IYISTDKAANPSNFYGMTKAIGEKLFVYANLLGSN--TRFVTVRGGNVLGTNGSVVHLFM 177 I +STDKA NP N YG TK +KLFV AN + N TRF R GNV+G+ GSVV F Sbjct: 121 IALSTDKACNPVNLYGATKLASDKLFVAANNIAGNKQTRFGVTRYGNVVGSRGSVVPFFK 180 Query: 178 KQIKDKG-QVRITDMKMTRFFFTLRDAITLLFKASEVSLGGEIFVMTMPTCRIVDLAEVL 236 K I + ++ ITD +MTRF+ +L D + + E GGEIF+ +P+ +I DLA L Sbjct: 181 KLISEGAKELPITDTRMTRFWISLEDGVKFVLSNFERMHGGEIFIPKIPSMKITDLAHAL 240 Query: 237 IEASGRRDVSIIEAGIRPGEKIHEILMSDFESQTTVVYDEQYLVILPTL 285 + + GIR GEK+HEI++S +S T + E Y I P++ Sbjct: 241 APNLSHKII-----GIRAGEKLHEIMISSDDSHLTYEF-ENYYAISPSI 283 >PM1007 [MG] COG1086 Predicted nucleoside-diphosphate sugar epimerases Length = 344 Score = 230 bits (586), Expect = 2e-60, Method: Compositional matrix adjust. Identities = 136/341 (39%), Positives = 203/341 (59%), Gaps = 22/341 (6%) Query: 1 MFNNKRILVTGGTGSWGHELIRQLLPQNPKEIVIFSRSESAQVAMSREYEDSRLSFIIGD 60 MF NK +L+TGGTGS+G+ ++++ L + +EI +FSR E Q M ++Y D++L F IGD Sbjct: 1 MFKNKTLLITGGTGSFGNAVLKRFLETDIREIRVFSRDEKKQDDMRKKYNDAKLKFYIGD 60 Query: 61 IRDKDALVAACRGIDYVFHLAALKHVPVCEDQPYEALKTNVVGTQNVIEAAIACNVEKAI 120 +RD D+++ A RG+DY++H AALK VP CE P EA+KTN++GT NV+EAAI +++ + Sbjct: 61 VRDYDSILNASRGVDYIYHAAALKQVPSCEFYPLEAVKTNILGTANVLEAAIQNQIKRVV 120 Query: 121 YISTDKAANPSNFYGMTKAIGEKLFVYA--NLLGSNTRFVTVRGGNVLGTNGSVVHLFMK 178 +STDKA P N G++KA+ EK+ + NL G+ T R GNV+ + GSV+ LF+ Sbjct: 121 CLSTDKAVYPINAMGISKAMMEKVIIAKSRNLEGTPTTICCTRYGNVMASRGSVIPLFVD 180 Query: 179 QIKDKGQVRITDMKMTRFFFTLRDAITLLFKASEVSLGGEIFVMTMPTCRIVDLAEVLIE 238 QI+ ITD +MTRF TL DA+ L+ A + G++FV P I LA+ + E Sbjct: 181 QIRQGKPFTITDPEMTRFMMTLEDAVDLVLYAFKNGQNGDVFVQKAPAATIGTLAKAITE 240 Query: 239 --ASGRRDVSIIEAGIRPGEKIHEILMSDFESQTTVVYDEQYLVILPTLDMPELKARYSK 296 + +SII G R GEK E L+S E + + Y I D L YSK Sbjct: 241 LLSVPNHPISII--GTRHGEKAFEALLSR-EEMVHAINEGNYYRI--PADQRSLN--YSK 293 Query: 297 Y-----PHVS----FNSFSSENNLMDQREIKDILIRGGFLQ 328 Y P ++ +NS ++E + +E+K +L++ F+Q Sbjct: 294 YVEKGEPKITEVTDYNSHNTER--LTVKEMKQLLLKLEFIQ 332 >SA0148 [MG] COG1086 Predicted nucleoside-diphosphate sugar epimerases Length = 342 Score = 220 bits (561), Expect = 2e-57, Method: Compositional matrix adjust. Identities = 120/266 (45%), Positives = 171/266 (64%), Gaps = 3/266 (1%) Query: 1 MFNNKRILVTGGTGSWGHELIRQLLPQNPKEIVIFSRSESAQVAMSREYEDSRLSFIIGD 60 MF++K +L+TGGTGS+G+ ++++ L N KEI IFSR E Q + ++Y +S+L F IGD Sbjct: 1 MFDDKILLITGGTGSFGNAVMKRFLDSNIKEIRIFSRDEKKQDDIRKKYNNSKLKFYIGD 60 Query: 61 IRDKDALVAACRGIDYVFHLAALKHVPVCEDQPYEALKTNVVGTQNVIEAAIACNVEKAI 120 +RD ++ A R +DYVFH AALK VP CE P EA+KTN++GT+NV+++AI NV+K I Sbjct: 61 VRDSQSVETAMRDVDYVFHAAALKQVPSCEFFPVEAVKTNIIGTENVLQSAIHQNVKKVI 120 Query: 121 YISTDKAANPSNFYGMTKAIGEKLFVYA--NLLGSNTRFVTVRGGNVLGTNGSVVHLFMK 178 +STDKAA P N G++KA+ EK+FV N+ T R GNV+ + GSV+ LF+ Sbjct: 121 CLSTDKAAYPINAMGISKAMMEKVFVAKSRNIRSEQTLICGTRYGNVMASRGSVIPLFID 180 Query: 179 QIKDKGQVRITDMKMTRFFFTLRDAITLLFKASEVSLGGEIFVMTMPTCRIVDLAEVLIE 238 +IK + ITD MTRF +L DA+ L+ A + + G+I V P+ + DLA L+E Sbjct: 181 KIKAGEPLTITDPDMTRFLMSLEDAVELVVHAFKHAETGDIMVQKAPSSTVGDLATALLE 240 Query: 239 ASGRRDVSIIEAGIRPGEKIHEILMS 264 D +I G R GEK E L++ Sbjct: 241 LF-EADNAIEIIGTRHGEKKAETLLT 265 >CC0233 [MG] COG1086 Predicted nucleoside-diphosphate sugar epimerases Length = 363 Score = 220 bits (561), Expect = 2e-57, Method: Compositional matrix adjust. Identities = 123/329 (37%), Positives = 196/329 (59%), Gaps = 32/329 (9%) Query: 2 FNNKRILVTGGTGSWGHELIRQLLPQ-NPKEIVIFSRSESAQVAMSREYED-------SR 53 + K ILVTGGTGS+G I +L + +P++++++SR E Q M E + ++ Sbjct: 29 LDGKVILVTGGTGSFGRRFIETVLRRYDPRKVIVYSRDELKQSDMQIELREQFDEATVAK 88 Query: 54 LSFIIGDIRDKDALVAACRGIDYVFHLAALKHVPVCEDQPYEALKTNVVGTQNVIEAAIA 113 + F +GD+RD++ L A RG+D V H AALK VP E P E + TNV+G +NV+ A++A Sbjct: 89 MRFFLGDVRDRERLTLALRGVDIVIHAAALKQVPAAEYNPSECIHTNVLGAENVVWASLA 148 Query: 114 CNVEKAIYISTDKAANPSNFYGMTKAIGEKLFVYANLLGSN--TRFVTVRGGNVLGTNGS 171 V++ + +STDKA NP+N YG TK +K FV AN L + TRF VR GNV+G+ GS Sbjct: 149 NAVKQVVALSTDKACNPTNLYGATKLASDKTFVAANNLSGDIGTRFCVVRYGNVVGSRGS 208 Query: 172 VVHLFMKQIKDKG-QVRITDMKMTRFFFTLRDAITLLFKASEVSLGGEIFVMTMPTCRIV 230 VV L+ + + ++ +TD +MTRF+ TL + + + + + GGEIFV +P+ + Sbjct: 209 VVPLYRRLLSQGATELPVTDPRMTRFWITLNEGVDFVLSSLTMMRGGEIFVPKIPSMAMP 268 Query: 231 DLAEVLIEASGRRDVSIIEAGIRPGEKIHEILMSDFESQTTVVYDEQYLVILPTLDMPEL 290 DL + + + + + GIRPGEK+HEI++S ++++TV +D++Y I P Sbjct: 269 DLVKAMSSTAAMKVI-----GIRPGEKLHEIMISADDARSTVEFDDRY-AIEPNF----- 317 Query: 291 KARYSKYPHVSFN---------SFSSENN 310 A + + P+ + + S+SS+NN Sbjct: 318 -AEFGREPYAASDGAKPVAEDFSYSSDNN 345 Database: COG Posted date: Aug 3, 2010 9:58 AM Number of letters in database: 38,378,431 Number of sequences in database: 115,334 Lambda K H 0.322 0.137 0.390 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 115334 Number of Hits to DB: 23,225,682 Number of extensions: 916201 Number of successful extensions: 2228 Number of sequences better than 1.0e-05: 84 Number of HSP's gapped: 2197 Number of HSP's successfully gapped: 84 Length of query: 328 Length of database: 38,378,431 Length adjustment: 107 Effective length of query: 221 Effective length of database: 26,037,693 Effective search space: 5754330153 Effective search space used: 5754330153 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.4 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.9 bits) S2: 116 (49.3 bits)