BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0021.0010.p (232 letters) Database: COG 115,334 sequences; 38,378,431 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value RP339 [M] COG0463 Glycosyltransferases involved in cell wall bio... 129 3e-30 RC0461 [M] COG0463 Glycosyltransferases involved in cell wall bi... 124 9e-29 HI0868 [M] COG0463 Glycosyltransferases involved in cell wall bi... 89 4e-18 BH3661 [M] COG0463 Glycosyltransferases involved in cell wall bi... 89 8e-18 PAB0772 [M] COG0463 Glycosyltransferases involved in cell wall b... 88 9e-18 >RP339 [M] COG0463 Glycosyltransferases involved in cell wall biogenesis Length = 318 Score = 129 bits (325), Expect = 3e-30, Method: Compositional matrix adjust. Identities = 77/185 (41%), Positives = 109/185 (58%), Gaps = 14/185 (7%) Query: 1 VVIPFYN-CPYIEQALQSALNQSWQPYEIIVVDDGSTMHTDRIT-PYLPHIHYLGKANGG 58 ++IP YN Y+ +A+ SAL Q+++ EIIVV+DGS T+ I Y I YL K NGG Sbjct: 12 IIIPVYNGANYMREAIDSALAQTYKNIEIIVVNDGSKDETETIALSYGDKICYLYKENGG 71 Query: 59 TASALNHGIRHATGDYVVWLSSDDMFYRDKINNQVLFMDQ--NRLLITYTNFNYINESTQ 116 SALN GI++ G Y WLS DD++Y +KI +Q+ +++ N+ +I Y + I++ + Sbjct: 72 CGSALNCGIKNMKGKYFSWLSHDDVYYPNKIEHQINILNKLDNKDVIVYCGYELIDQKSH 131 Query: 117 LTELNAAAVFPSH------LDYLRCFLQGNPINGCTVMFKRELFAAIGLFDESLPYTHDY 170 + V P LD L + I+GCT++ LF IGLFDESL YTHDY Sbjct: 132 ----SLYCVKPDQRYSKEKLDISLFPLLHSLIHGCTLLIPSILFQKIGLFDESLKYTHDY 187 Query: 171 DLWFR 175 DLWF+ Sbjct: 188 DLWFK 192 >RC0461 [M] COG0463 Glycosyltransferases involved in cell wall biogenesis Length = 604 Score = 124 bits (312), Expect = 9e-29, Method: Compositional matrix adjust. Identities = 78/212 (36%), Positives = 122/212 (57%), Gaps = 11/212 (5%) Query: 1 VVIPFYN-CPYIEQALQSALNQSWQPYEIIVVDDGST--MHTDRIT-PYLPHIHYLGKAN 56 ++IP YN Y+++A+ SAL Q+++ EIIVV+DGS T+R+ Y I Y K N Sbjct: 18 IIIPVYNGANYMKEAINSALAQTYKNIEIIVVNDGSKDNGETERVALSYGDKIRYFYKEN 77 Query: 57 GGTASALNHGIRHATGDYVVWLSSDDMFYRDKINNQVLFMDQ--NRLLITYTNFNYINES 114 GG SALN+GI++ G+Y WLS DD++Y +KI +QV +++ N+ I Y + I+E Sbjct: 78 GGCGSALNYGIKNMQGEYFSWLSHDDIYYPNKIEHQVDILNKLDNKDTIIYGGYELIDEK 137 Query: 115 -TQLTELNAAAVFP-SHLDYLRCFLQGNPINGCTVMFKRELFAAIGLFDESLPYTHDYDL 172 L + +V P + L+ L I+GC+++ + F +G+F+E+LP T DYDL Sbjct: 138 GNSLRYIKPDSVLPINKLNISLLPLLRGLIHGCSLLMPAKYFHEVGIFNEALPTTQDYDL 197 Query: 173 WFRAILNGYPPVMLNQS-LTAYRRHDGMGTLK 203 WF+ P+ ++S L R H G+ K Sbjct: 198 WFKIF--RVAPIHFDESILIKSRFHSEQGSKK 227 Score = 103 bits (256), Expect = 3e-22, Method: Compositional matrix adjust. Identities = 71/211 (33%), Positives = 110/211 (52%), Gaps = 18/211 (8%) Query: 1 VVIPFYN-CPYIEQALQSALNQSWQPYEIIVVDDGSTMHTDRITPYLP---HIHYLGKAN 56 V+IP YN + +A++S L Q+ + +EI+++DDGST +T I Y K N Sbjct: 297 VIIPVYNRINWAIEAIKSVLIQTHKNFEILIIDDGSTDDISELTAICKKDKRIKYFHKKN 356 Query: 57 GGTASALNHGIRHATGDYVVWLSSDDMFYRDKINNQVLFMDQNRLLITYTNFNYINESTQ 116 G A+A N GI++A G Y+ +L SDD+FY+DKI Q+ FM++N + ++T+++ INE + Sbjct: 357 EGPAAARNLGIKNAIGKYIAFLDSDDLFYKDKIEIQLKFMEENNFIFSHTSYHKINEKGK 416 Query: 117 LTE-----LNAAAVFPSHLDYLRCFLQGNPINGCTVMFKRELFAAIGLFDESLPYTHDYD 171 E L + VFP +Q PI TVM LF LF E++ D Sbjct: 417 YIESVHSGLFSGNVFPQ-------VIQTCPIAMPTVMGTLTLFQE-NLFPENIRSGEDCC 468 Query: 172 LWFRAILNGYPPVMLNQSLTAYRRHDGMGTL 202 LW +I + +++ L+ R G T Sbjct: 469 LWI-SIASKNSIGGIDKELSKVRISGGTNTF 498 >HI0868 [M] COG0463 Glycosyltransferases involved in cell wall biogenesis Length = 250 Score = 89.4 bits (220), Expect = 4e-18, Method: Compositional matrix adjust. Identities = 56/159 (35%), Positives = 86/159 (54%), Gaps = 12/159 (7%) Query: 1 VVIPFYNCP-YIEQALQSALNQSWQPYEIIVVDDGSTMHTDRITPYLPHIH-----YLGK 54 +++P YN Y+ Q + S LNQS+Q E+I++DDGST + I + + Sbjct: 8 IIMPVYNAECYLNQGILSCLNQSYQNIELILIDDGSTDKSIEIINNIIDKDKRVKLFFTP 67 Query: 55 ANGGTASALNHGIRHATGDYVVWLSSDDMFYRDKINNQVLFMDQNRLLITYTNFNYIN-E 113 N G A+A N G+ A GDY+ +L SDD DK+ Q+ FM QN L++T+ N+ + + E Sbjct: 68 TNQGPAAARNIGLEKAQGDYITFLDSDDFIANDKLEKQLNFMLQNHLVMTHGNYAFCDLE 127 Query: 114 STQLTELNAAAVFPSHLDYLRCFLQGNPINGCTVMFKRE 152 Q+ + + +DYL LQGN TV+ +RE Sbjct: 128 GNQIKLVTTS----KKIDYL-TLLQGNQFKIMTVLVERE 161 >BH3661 [M] COG0463 Glycosyltransferases involved in cell wall biogenesis Length = 257 Score = 88.6 bits (218), Expect = 8e-18, Method: Compositional matrix adjust. Identities = 58/200 (29%), Positives = 97/200 (48%), Gaps = 11/200 (5%) Query: 1 VVIPFYNCP-YIEQALQSALNQSWQPYEIIVVDDGSTMHTDRITP-YLPHIHYLG----K 54 ++ P YN +I ++SALNQ++ +E+++VDDGST T +I Y + + Sbjct: 13 IITPCYNAEMFIRDTIESALNQTFSNWEMVIVDDGSTDRTVQIVESYASQDERIRLIQLE 72 Query: 55 ANGGTASALNHGIRHATGDYVVWLSSDDMFYRDKINNQVLFMDQNRLLITYTNFNYINES 114 N G A + N I+HA G Y+ +L SDD + +K+ Q+ FM + + ++T++ + E Sbjct: 73 KNSGPAVSRNTAIQHARGRYLAFLDSDDQWLPEKLERQLEFMQKRNVAFSFTSYKTMTED 132 Query: 115 TQLTELNAAAVFPSHLDYLRCFLQGNPINGCTVMFKRELFAAIGLFDESLPYTHDYDLWF 174 +E P ++DY L+ N I TVM +E + + + + DY LW Sbjct: 133 G--SETGKVVNVPENVDYHE-LLKQNVIGCLTVMLDQEKTGHVQMVN--MRSRQDYALWL 187 Query: 175 RAILNGYPPVMLNQSLTAYR 194 G+ L L YR Sbjct: 188 HLCKRGFIAYGLQDVLAKYR 207 >PAB0772 [M] COG0463 Glycosyltransferases involved in cell wall biogenesis Length = 298 Score = 88.2 bits (217), Expect = 9e-18, Method: Compositional matrix adjust. Identities = 57/182 (31%), Positives = 89/182 (48%), Gaps = 11/182 (6%) Query: 1 VVIPFYN-CPYIEQALQSALNQSWQPYEIIVVDDGSTMHTDRITPYLP--HIHYLG-KAN 56 V+IP YN + +A+ S LNQ ++ +E+IVVDD ST +T + + I Y+ K N Sbjct: 8 VIIPTYNRANLLRRAIASVLNQKFKDFELIVVDDASTDNTPEVVESIEDGRIRYIRLKKN 67 Query: 57 GGTASALNHGIRHATGDYVVWLSSDDMFYRDKINNQVLFMDQ--NRLLITYTNFNYINES 114 G A N GI+ A G ++ L DD + ++ QV + + Y F Y+++ Sbjct: 68 SGGPIARNIGIKKAKGRFIALLDDDDEWLPHRLEVQVRKFENLGKEFGVVYGGFYYVSQD 127 Query: 115 TQLTELNAAAVFPSHL-DYLRCFLQGNPINGCTVMFKRELFAAIGLFDESLPYTHDYDLW 173 ++ P H D L+ N I T++ +RE F GLFD L + D+D+W Sbjct: 128 GRIL----GKRLPKHRGDIYSHLLKENFIGSPTLLIRRECFKKAGLFDPRLSSSQDWDMW 183 Query: 174 FR 175 R Sbjct: 184 LR 185 Database: COG Posted date: Aug 3, 2010 9:58 AM Number of letters in database: 38,378,431 Number of sequences in database: 115,334 Lambda K H 0.323 0.138 0.429 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 115334 Number of Hits to DB: 17,920,019 Number of extensions: 741359 Number of successful extensions: 1368 Number of sequences better than 1.0e-05: 75 Number of HSP's gapped: 1354 Number of HSP's successfully gapped: 76 Length of query: 232 Length of database: 38,378,431 Length adjustment: 103 Effective length of query: 129 Effective length of database: 26,499,029 Effective search space: 3418374741 Effective search space used: 3418374741 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.5 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (22.0 bits) S2: 114 (48.5 bits)