BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0021.0008.p (240 letters) Database: COG 115,334 sequences; 38,378,431 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value RC0461 [M] COG0463 Glycosyltransferases involved in cell wall bi... 135 4e-32 RP339 [M] COG0463 Glycosyltransferases involved in cell wall bio... 132 4e-31 PAB0772 [M] COG0463 Glycosyltransferases involved in cell wall b... 117 1e-26 PH0401 [M] COG0463 Glycosyltransferases involved in cell wall bi... 106 2e-23 BH3713 [M] COG0463 Glycosyltransferases involved in cell wall bi... 102 6e-22 >RC0461 [M] COG0463 Glycosyltransferases involved in cell wall biogenesis Length = 604 Score = 135 bits (341), Expect = 4e-32, Method: Compositional matrix adjust. Identities = 87/215 (40%), Positives = 124/215 (57%), Gaps = 10/215 (4%) Query: 3 YGTRVSVIIPFYN-CSYVHLAIESVLAQSYPDIEIIVVDDGSTLH--TEKL-LPYSGRIK 58 Y T VS+IIP YN +Y+ AI S LAQ+Y +IEIIVV+DGS + TE++ L Y +I+ Sbjct: 12 YHTLVSIIIPVYNGANYMKEAINSALAQTYKNIEIIVVNDGSKDNGETERVALSYGDKIR 71 Query: 59 VLRKANGGTASALNLGITSASGAYFAWLSADDCFHPDKIGRQIAVM--LATQTSFNHTAY 116 K NGG SALN GI + G YF+WLS DD ++P+KI Q+ ++ L + + + Y Sbjct: 72 YFYKENGGCGSALNYGIKNMQGEYFSWLSHDDIYYPNKIEHQVDILNKLDNKDTIIYGGY 131 Query: 117 YYINELGE--RSSGRISV-PFHSKAELIETLMTGCPVNGSTVMVDMNVFKKVGLFNEKLL 173 I+E G R SV P + + L+ G ++G ++++ F +VG+FNE L Sbjct: 132 ELIDEKGNSLRYIKPDSVLPINKLNISLLPLLRGL-IHGCSLLMPAKYFHEVGIFNEALP 190 Query: 174 YTQDYDLWLRILAYYEWSYIEDPLLDYRVHQEMGS 208 TQDYDLW +I + E L+ R H E GS Sbjct: 191 TTQDYDLWFKIFRVAPIHFDESILIKSRFHSEQGS 225 Score = 112 bits (279), Expect = 7e-25, Method: Compositional matrix adjust. Identities = 73/202 (36%), Positives = 109/202 (53%), Gaps = 7/202 (3%) Query: 5 TRVSVIIPFYN-CSYVHLAIESVLAQSYPDIEIIVVDDGSTLHTEKLLPY---SGRIKVL 60 T++SVIIP YN ++ AI+SVL Q++ + EI+++DDGST +L RIK Sbjct: 293 TKISVIIPVYNRINWAIEAIKSVLIQTHKNFEILIIDDGSTDDISELTAICKKDKRIKYF 352 Query: 61 RKANGGTASALNLGITSASGAYFAWLSADDCFHPDKIGRQIAVMLATQTSFNHTAYYYIN 120 K N G A+A NLGI +A G Y A+L +DD F+ DKI Q+ M F+HT+Y+ IN Sbjct: 353 HKKNEGPAAARNLGIKNAIGKYIAFLDSDDLFYKDKIEIQLKFMEENNFIFSHTSYHKIN 412 Query: 121 ELGERSSGRISVPFHSKAELIETLMTGCPVNGSTVMVDMNVFKKVGLFNEKLLYTQDYDL 180 E G+ S F + ++ CP+ TVM + +F++ LF E + +D L Sbjct: 413 EKGKYIESVHSGLF--SGNVFPQVIQTCPIAMPTVMGTLTLFQE-NLFPENIRSGEDCCL 469 Query: 181 WLRILAYYEWSYIEDPLLDYRV 202 W+ I + I+ L R+ Sbjct: 470 WISIASKNSIGGIDKELSKVRI 491 >RP339 [M] COG0463 Glycosyltransferases involved in cell wall biogenesis Length = 318 Score = 132 bits (333), Expect = 4e-31, Method: Compositional matrix adjust. Identities = 80/207 (38%), Positives = 118/207 (57%), Gaps = 6/207 (2%) Query: 3 YGTRVSVIIPFYN-CSYVHLAIESVLAQSYPDIEIIVVDDGSTLHTEKL-LPYSGRIKVL 60 Y VS+IIP YN +Y+ AI+S LAQ+Y +IEIIVV+DGS TE + L Y +I L Sbjct: 6 YSPLVSIIIPVYNGANYMREAIDSALAQTYKNIEIIVVNDGSKDETETIALSYGDKICYL 65 Query: 61 RKANGGTASALNLGITSASGAYFAWLSADDCFHPDKIGRQIAVM--LATQTSFNHTAYYY 118 K NGG SALN GI + G YF+WLS DD ++P+KI QI ++ L + + Y Sbjct: 66 YKENGGCGSALNCGIKNMKGKYFSWLSHDDVYYPNKIEHQINILNKLDNKDVIVYCGYEL 125 Query: 119 INELGERSSGRISVPFHSKAELIETL--MTGCPVNGSTVMVDMNVFKKVGLFNEKLLYTQ 176 I++ +SK +L +L + ++G T+++ +F+K+GLF+E L YT Sbjct: 126 IDQKSHSLYCVKPDQRYSKEKLDISLFPLLHSLIHGCTLLIPSILFQKIGLFDESLKYTH 185 Query: 177 DYDLWLRILAYYEWSYIEDPLLDYRVH 203 DYDLW + + + L+ R+H Sbjct: 186 DYDLWFKFFRVSSIYFDHEVLIKSRIH 212 >PAB0772 [M] COG0463 Glycosyltransferases involved in cell wall biogenesis Length = 298 Score = 117 bits (294), Expect = 1e-26, Method: Compositional matrix adjust. Identities = 72/203 (35%), Positives = 114/203 (56%), Gaps = 9/203 (4%) Query: 7 VSVIIPFYN-CSYVHLAIESVLAQSYPDIEIIVVDDGSTLHTEKLLPY--SGRIKVLR-K 62 VSVIIP YN + + AI SVL Q + D E+IVVDD ST +T +++ GRI+ +R K Sbjct: 6 VSVIIPTYNRANLLRRAIASVLNQKFKDFELIVVDDASTDNTPEVVESIEDGRIRYIRLK 65 Query: 63 ANGGTASALNLGITSASGAYFAWLSADDCFHPDKIGRQIAVM--LATQTSFNHTAYYYIN 120 N G A N+GI A G + A L DD + P ++ Q+ L + + +YY++ Sbjct: 66 KNSGGPIARNIGIKKAKGRFIALLDDDDEWLPHRLEVQVRKFENLGKEFGVVYGGFYYVS 125 Query: 121 ELGERSSGRISVPFHSKAELIETLMTGCPVNGSTVMVDMNVFKKVGLFNEKLLYTQDYDL 180 + G R+ P H + ++ L+ + T+++ FKK GLF+ +L +QD+D+ Sbjct: 126 QDGRILGKRL--PKH-RGDIYSHLLKENFIGSPTLLIRRECFKKAGLFDPRLSSSQDWDM 182 Query: 181 WLRILAYYEWSYIEDPLLDYRVH 203 WLRI YY++ Y+++ + Y VH Sbjct: 183 WLRIARYYKFDYVDEIIAKYYVH 205 >PH0401 [M] COG0463 Glycosyltransferases involved in cell wall biogenesis Length = 294 Score = 106 bits (265), Expect = 2e-23, Method: Compositional matrix adjust. Identities = 77/211 (36%), Positives = 118/211 (55%), Gaps = 23/211 (10%) Query: 6 RVSVIIPFYNCS-YVHLAIESVLAQSYPDIEIIVVDDGSTLHTEKLLPY--SGRIKVLRK 62 R+SVIIP YN + AIESVL Q++ D EI+V+D + T +L+ GRI+ + + Sbjct: 4 RISVIIPTYNREELLRRAIESVLDQTFDDFEILVIDGAKSESTRELVRSFGDGRIRYIPQ 63 Query: 63 ANGGTASALNLGITSASGAYFAWLSADDCFHPDKIGRQIAVMLATQTSFN--HTA--YYY 118 G A+A NLG++ A G + A+L DD + DK+ Q+ + S+ +TA YYY Sbjct: 64 KGKGIANARNLGVSKARGEFIAFLDDDDRWRRDKLELQLELFKVLPQSYGVVYTAFTYYY 123 Query: 119 INE---LG---ERSSGRISVPFHSKAELIETLMTGCPVNGSTVMVDMNVFKKVGLFNEKL 172 + + LG R+SG + L++ +TG ST+MV FKKVGLF E Sbjct: 124 LEKDKVLGIKHPRASGDVY------KHLLKDNITGT----STIMVKRECFKKVGLFREDF 173 Query: 173 LYTQDYDLWLRILAYYEWSYIEDPLLDYRVH 203 + +D+D+WLR+ + I++PL+DY +H Sbjct: 174 VTCEDWDMWLRMAKVCYFGAIDEPLVDYSIH 204 >BH3713 [M] COG0463 Glycosyltransferases involved in cell wall biogenesis Length = 303 Score = 102 bits (253), Expect = 6e-22, Method: Compositional matrix adjust. Identities = 69/202 (34%), Positives = 103/202 (50%), Gaps = 8/202 (3%) Query: 6 RVSVIIPFYN-CSYVHLAIESVLAQSYPDIEIIVVDDGSTLHTEKLL---PYSGRIKVL- 60 +VS+IIP +N + A+ES L Q+Y +IE+IVV DGST +T+ ++ R+ + Sbjct: 2 KVSIIIPTHNRAKLLKRALESTLNQTYKNIEVIVVSDGSTDNTDIVMDEYKRDSRVNFIS 61 Query: 61 -RKANGGTASALNLGITSASGAYFAWLSADDCFHPDKIGRQIAVM-LATQTSFNHTAYYY 118 A GG A N GI +A G + A+L DD + PDK+ QI +T Sbjct: 62 YHPAKGGNY-ARNTGIKNAKGEFIAFLDDDDEWMPDKLELQIKEFNQNANVGLVYTGVEI 120 Query: 119 INELGERSSGRISVPFHSKAELIETLMTGCPVNGSTVMVDMNVFKKVGLFNEKLLYTQDY 178 I +R+ S+P + E L+ C S+VMV N+ + G+F+EKL QDY Sbjct: 121 IYNFNKRNIKYYSLPKKTGNLSKEILVANCIGTTSSVMVRKNLITECGMFDEKLKARQDY 180 Query: 179 DLWLRILAYYEWSYIEDPLLDY 200 DLW+R+ + PL+ Y Sbjct: 181 DLWIRVCQKTLVGVVNKPLVRY 202 Database: COG Posted date: Aug 3, 2010 9:58 AM Number of letters in database: 38,378,431 Number of sequences in database: 115,334 Lambda K H 0.321 0.136 0.401 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 115334 Number of Hits to DB: 17,555,380 Number of extensions: 677823 Number of successful extensions: 1746 Number of sequences better than 1.0e-05: 80 Number of HSP's gapped: 1682 Number of HSP's successfully gapped: 81 Length of query: 240 Length of database: 38,378,431 Length adjustment: 103 Effective length of query: 137 Effective length of database: 26,499,029 Effective search space: 3630366973 Effective search space used: 3630366973 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.4 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.9 bits) S2: 114 (48.5 bits)