BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0021.0003.p (242 letters) Database: COG 115,334 sequences; 38,378,431 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value BS_nagB [G] COG0363 6-phosphogluconolactonase/Glucosamine-6-phos... 235 5e-62 BS_ybfT [G] COG0363 6-phosphogluconolactonase/Glucosamine-6-phos... 232 5e-61 L14408 [G] COG0363 6-phosphogluconolactonase/Glucosamine-6-phosp... 218 6e-57 lin0956 [G] COG0363 6-phosphogluconolactonase/Glucosamine-6-phos... 209 3e-54 VCA1025 [G] COG0363 6-phosphogluconolactonase/Glucosamine-6-phos... 197 1e-50 >BS_nagB [G] COG0363 6-phosphogluconolactonase/Glucosamine-6-phosphate isomerase/deaminase Length = 242 Score = 235 bits (599), Expect = 5e-62, Method: Compositional matrix adjust. Identities = 113/238 (47%), Positives = 157/238 (65%) Query: 1 MNILKFTSDEDFAQTGANLIVSLLQSNPKAVLGLATGSSPVGVYAKLVEMHAKGIVSFAK 60 M +++ + E+ +Q A + ++ P AVLGLATG +P G Y +L+ +H +SF Sbjct: 1 MKVMECQTYEELSQIAARITADTIKEKPDAVLGLATGGTPEGTYRQLIRLHQTENLSFQN 60 Query: 61 ASSYNLDEYVGLPVDHPQSYRSFMNEHLFNHIDIDLAKTHVPNGNAADLEAECLAYDKLL 120 ++ NLDEY GL D P SY +MN+ F HID ++ +PNGNA DLEAEC Y++L+ Sbjct: 61 ITTVNLDEYAGLSSDDPNSYHFYMNDRFFQHIDSKPSRHFIPNGNADDLEAECRRYEQLV 120 Query: 121 EEGGPVDLQILGIGSNGHIGFNEPDASLTSGTHVVDLLPETLEANARFFDSREDVPRQAV 180 + G D+Q+LGIG NGHIGFNEP S S THVV L +T +ANAR+F S + VP++A+ Sbjct: 121 DSLGDTDIQLLGIGRNGHIGFNEPGTSFKSRTHVVTLNEQTRQANARYFPSIDSVPKKAL 180 Query: 181 TMGIGGILKARQIVLLVRGEEKAEAVKNALEGPITTQCPASLLQSHPNVVVLLDEGAA 238 TMGI IL +++I+LL+ G+ KAEAV+ LEG I+ PAS L H +V VL+D AA Sbjct: 181 TMGIQTILSSKRILLLISGKSKAEAVRKLLEGNISEDFPASALHLHSDVTVLIDREAA 238 >BS_ybfT [G] COG0363 6-phosphogluconolactonase/Glucosamine-6-phosphate isomerase/deaminase Length = 249 Score = 232 bits (591), Expect = 5e-61, Method: Compositional matrix adjust. Identities = 117/244 (47%), Positives = 160/244 (65%), Gaps = 2/244 (0%) Query: 1 MNILKFTSDEDFAQTGANLIVSLLQSNPKAVLGLATGSSPVGVYAKLVEMHAKGIVSFAK 60 M IL E+ + A +I +Q+ AVLGLATGS+PVG+Y +L+ + G + F+K Sbjct: 1 MKILIAEHYEELCKLSAAIIKEQIQAKKDAVLGLATGSTPVGLYKQLISDYQAGEIDFSK 60 Query: 61 ASSYNLDEYVGLPVDHPQSYRSFMNEHLFNHIDIDLAKTHVPNGNAADLEAECLAYDKLL 120 +++NLDEY GL HPQSY FM+EHLF HI++ H+P G+ LEA C Y+ L+ Sbjct: 61 VTTFNLDEYAGLSPSHPQSYNHFMHEHLFQHINMQPDHIHIPQGDNPQLEAACKVYEDLI 120 Query: 121 EEGGPVDLQILGIGSNGHIGFNEPDASLTSGTHVVDLLPETLEANARFFDSRED-VPRQA 179 + G +D+QILGIG+NGHIGFNEP + T VV L T++ANARFF VPR A Sbjct: 121 RQAGGIDVQILGIGANGHIGFNEPGSDFEDRTRVVKLSESTIQANARFFGGDPVLVPRLA 180 Query: 180 VTMGIGGILK-ARQIVLLVRGEEKAEAVKNALEGPITTQCPASLLQSHPNVVVLLDEGAA 238 ++MGI I++ ++ IVLL GEEKA+A++ EGP+TT PAS+LQ H +V V+ D AA Sbjct: 181 ISMGIKTIMEFSKHIVLLASGEEKADAIQKMAEGPVTTDVPASILQKHNHVTVIADYKAA 240 Query: 239 KWLK 242 + LK Sbjct: 241 QKLK 244 >L14408 [G] COG0363 6-phosphogluconolactonase/Glucosamine-6-phosphate isomerase/deaminase Length = 235 Score = 218 bits (555), Expect = 6e-57, Method: Compositional matrix adjust. Identities = 112/216 (51%), Positives = 145/216 (67%), Gaps = 7/216 (3%) Query: 26 SNPKAVLGLATGSSPVGVYAKLVEMHAKGIVSFAKASSYNLDEYVGLPVDHPQSYRSFMN 85 +N LGLATGS+PV Y ++V + F S NLDEYVGL + QSYR FM Sbjct: 25 ANGAKTLGLATGSTPVEFYNQIVNSD----LDFTDMVSVNLDEYVGLDGSNDQSYRYFMT 80 Query: 86 EHLFNHIDIDLAKTHVPNGNAADLEAECLAYDKLLEEGGPVDLQILGIGSNGHIGFNEPD 145 +HLF + + +PNG AADLEAE YD+++EE P+D QILGIG NGHIGFNEP Sbjct: 81 KHLFG--EKPFKENFLPNGKAADLEAEAKHYDQIIEEN-PIDWQILGIGQNGHIGFNEPG 137 Query: 146 ASLTSGTHVVDLLPETLEANARFFDSREDVPRQAVTMGIGGILKARQIVLLVRGEEKAEA 205 THVVDL T++ANARFF+S DVPR+A++MG+ I+K++ IVL+ G+EKAEA Sbjct: 138 TPAEITTHVVDLQESTIKANARFFESEADVPRKAISMGLASIMKSKNIVLMAYGKEKAEA 197 Query: 206 VKNALEGPITTQCPASLLQSHPNVVVLLDEGAAKWL 241 +K +EG +TT+ PAS+LQ+H NV V+ DE A L Sbjct: 198 IKGMVEGEVTTELPASILQNHANVTVIADEAAVSLL 233 >lin0956 [G] COG0363 6-phosphogluconolactonase/Glucosamine-6-phosphate isomerase/deaminase Length = 301 Score = 209 bits (532), Expect = 3e-54, Method: Compositional matrix adjust. Identities = 16/211 (7%), Positives = 40/211 (18%), Gaps = 7/211 (3%) Query: 31 VLGLATGSSPVGVYAKLVEMHAKGIVSFAKASSYNLDEYVGLPVDHPQSYRSFMNEHLFN 90 T ++V A + Y + + + Sbjct: 31 QKISITSRKAQTTRHRIV----GIHTEGAYQAIYVDTPGLHMEEKRAINRLMNKAASSSI 86 Query: 91 HIDIDLAKTHVPNGNAADLEAECLAYDKLLEEGGPVDLQILGIGSNGHIGFNEPDASLTS 150 V G + E + + PV L + + + P + Sbjct: 87 GDV--ELVIFVVEGTRWTPDDEMVLNKLREGKA-PVILAVNKVDNVQEKADLLPHLQFLA 143 Query: 151 GTHVVDLLPETLEANARFFDSREDVPRQAVTMGIGGILKARQIVLLVRGEEKAEAVKNAL 210 + D+ + R+ + + R + + Sbjct: 144 SQMNFLDIVPISAETGLNVDTIAAIVRKHLPEATHHFPEDYITDRSQRFMASEIIREKLM 203 Query: 211 EGPITTQCPASLLQSHPNVVVLLDEGAAKWL 241 + ++ V L Sbjct: 204 RFLGAELPYSVTVEIERFVSNERGGYDINGL 234 >VCA1025 [G] COG0363 6-phosphogluconolactonase/Glucosamine-6-phosphate isomerase/deaminase Length = 266 Score = 197 bits (501), Expect = 1e-50, Method: Compositional matrix adjust. Identities = 104/239 (43%), Positives = 150/239 (62%), Gaps = 9/239 (3%) Query: 13 AQTG----ANLIVSLLQSNPKA----VLGLATGSSPVGVYAKLVEMHAKGIVSFAKASSY 64 AQ G A+++ + + P A VLGL TG +P+ Y L+EMH G VSF ++ Sbjct: 10 AQVGKWAAAHIVKRINEFQPTAERPFVLGLPTGGTPLATYKALIEMHKAGEVSFKHVVTF 69 Query: 65 NLDEYVGLPVDHPQSYRSFMNEHLFNHIDIDLAKTHVPNGNAADLEAECLAYDKLLEEGG 124 N+DEYVGL DHP+SYRSFM + FNHIDI ++ NGN D EAEC Y+ ++ G Sbjct: 70 NMDEYVGLAADHPESYRSFMYNNFFNHIDIQEENINLLNGNTDDHEAECKRYEDKIKSYG 129 Query: 125 PVDLQILGIGSNGHIGFNEPDASLTSGTHVVDLLPETLEANARFFDSR-EDVPRQAVTMG 183 ++L + G+G++GHI FNEP +SL+S T + L +T AN+RFFD VP+ A+T+G Sbjct: 130 KINLFMGGVGNDGHIAFNEPASSLSSRTRIKTLTEDTRIANSRFFDGDINQVPKYALTIG 189 Query: 184 IGGILKARQIVLLVRGEEKAEAVKNALEGPITTQCPASLLQSHPNVVVLLDEGAAKWLK 242 +G +L A++I++LV G KA A++ A+EG + S LQ HP V++ DE + + LK Sbjct: 190 VGTLLDAQEIMILVTGHNKALALQAAVEGSVNHLWTVSALQLHPKAVIVCDEPSTQELK 248 Database: COG Posted date: Aug 3, 2010 9:58 AM Number of letters in database: 38,378,431 Number of sequences in database: 115,334 Lambda K H 0.316 0.134 0.383 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 115334 Number of Hits to DB: 18,092,700 Number of extensions: 720289 Number of successful extensions: 1482 Number of sequences better than 1.0e-05: 23 Number of HSP's gapped: 1448 Number of HSP's successfully gapped: 23 Length of query: 242 Length of database: 38,378,431 Length adjustment: 103 Effective length of query: 139 Effective length of database: 26,499,029 Effective search space: 3683365031 Effective search space used: 3683365031 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.3 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.6 bits) S2: 114 (48.5 bits)