BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0020.0013.p (335 letters) Database: COG 115,334 sequences; 38,378,431 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value BH3794 [T] COG2206 HD-GYP domain 197 2e-50 BH0008 [T] COG2206 HD-GYP domain 164 2e-40 CAC2620 [T] COG2206 HD-GYP domain 154 3e-37 CAC0277 [T] COG2206 HD-GYP domain 143 4e-34 RSc2515 [T] COG2206 HD-GYP domain 133 3e-31 >BH3794 [T] COG2206 HD-GYP domain Length = 231 Score = 197 bits (501), Expect = 2e-50, Method: Compositional matrix adjust. Identities = 97/223 (43%), Positives = 144/223 (64%), Gaps = 1/223 (0%) Query: 110 AIQGKDDYTYQHNIGVGVLSTLIGRWLNMSESDLSVLSLAATLHDIGKLKIPSEILNKPG 169 I+ KDDYTY+H +GV +LS LIG WL + E L L+L A LHDIGK++IP ILNKPG Sbjct: 3 VIKAKDDYTYRHTVGVSILSRLIGTWLKLDEVILKELTLGAVLHDIGKVEIPDAILNKPG 62 Query: 170 KLTPDEYELVKKHTILGYEMLKETTGANSHITLTALQHHERNDGRGYPLGLKEEQIDAYA 229 LT DEY +++ HT+ GY +++E+ + + L AL+HHER DG GYP +I + Sbjct: 63 ALTKDEYRIIQDHTVKGYRIIQESGIYSDTVALMALEHHEREDGTGYPYQKTRNEIHLLS 122 Query: 230 KIVAVADIFHAMSSRRPYHEPVPFHIIVDQMRRGSFGALDPHIVSVFLENIVKRTVGREV 289 KI+AVAD+FHAM+S R Y + + + ++ QMR+ FG L+P I F+ +++ +G + Sbjct: 123 KILAVADVFHAMTSDRIYRKGMALYDVLLQMRQDRFGKLEPRITDFFVRRFMEQCIGSKA 182 Query: 290 ILTDGRVGEIVYLNPHDIETPLVKVDDEYIDLSKLNTIKIKEI 332 L DG EIV++ + P+VK YIDL +++ + I+E+ Sbjct: 183 RLNDGSRVEIVFIPYENPICPIVKQGTNYIDL-RMSPLYIQEL 224 >BH0008 [T] COG2206 HD-GYP domain Length = 360 Score = 164 bits (415), Expect = 2e-40, Method: Compositional matrix adjust. Identities = 88/270 (32%), Positives = 147/270 (54%), Gaps = 1/270 (0%) Query: 64 DVVNKSKDLFESFRISRKVPLADIRKEVVPAIQEISRHSDIFALFDAIQGKDDYTYQHNI 123 V K +F+ + V + +R ++P +EI D+ K+DY Y H + Sbjct: 88 QAVANYKKVFQEWAAGTPVEMECVRHILLPLFEEIVEKKDVLLQLHHYSTKEDYLYHHAV 147 Query: 124 GVGVLSTLIGRWLNMSESDLSVLSLAATLHDIGKLKIPSEILNKPGKLTPDEYELVKKHT 183 VG+LS +G L + + ++LA L D G K+P+ +L K G T E ++ H Sbjct: 148 SVGLLSAFLGAKLGYEKGEWLKIALAGALADCGMAKLPTSLLTKNGLYTLKEKHEMRDHP 207 Query: 184 ILGYEMLKETTGANSHITLTALQHHERNDGRGYPLGLKEEQIDAYAKIVAVADIFHAMSS 243 Y+MLK+ + + LQHHER DG GYPLG++ ++I YA+I+AVAD++HAM S Sbjct: 208 KYSYQMLKKRPEIDGSLLSAVLQHHEREDGTGYPLGVRGQKIHFYAQILAVADVYHAMIS 267 Query: 244 RRPYHEPVPFHIIVDQMRRGSFGALDPHIVSVFLENIVKRTVGREVILTDGRVGEIVYLN 303 R Y + +++++ + + FG +P IV V + + + +V V LT+ R+GEIV+++ Sbjct: 268 ERKYRKKKSPYMVLEDLAKERFGKFNPRIVHVLVSQLTQLSVRTSVRLTNNRLGEIVFID 327 Query: 304 PHDIETPLVKVDD-EYIDLSKLNTIKIKEI 332 P P+V++ E I L K T+ I+++ Sbjct: 328 PMAPTRPMVQLQTGEMIYLMKNYTVHIEDV 357 >CAC2620 [T] COG2206 HD-GYP domain Length = 345 Score = 154 bits (388), Expect = 3e-37, Method: Compositional matrix adjust. Identities = 76/220 (34%), Positives = 131/220 (59%), Gaps = 4/220 (1%) Query: 113 GKDDYTYQHNIGVGVLSTLIGRWLNMSESDLSVLSLAATLHDIGKLKIPSEILNKPGKLT 172 G + Y+H++ V +S ++G+WL M+E ++++L+ A+ LHD GK ++ + IL K LT Sbjct: 128 GSKNNIYRHSVNVAAISFILGKWLGMNEEEINLLTYASLLHDFGKTQLDNSILKKESSLT 187 Query: 173 PDEYELVKKHTILGYEMLKETTGANSHITLTALQHHERNDGRGYPLGLKEEQIDAYAKIV 232 P+EY + K H ++ Y ++KE N+ + L L HHE+ DG GYPLG+K+ +I +AKI+ Sbjct: 188 PEEYAVYKTHPVIAYHLIKEIPDINASVGLGVLMHHEQIDGSGYPLGIKDNKIHKFAKII 247 Query: 233 AVADIFHAMSSRRPYHEPVPFHIIVDQMRRGSFGALDPHIVSVFLENIVKRTVGREVILT 292 A+ADIF ++S PF + ++ S G LD ++F+ + V +G V L+ Sbjct: 248 AIADIFDKVNSDNTNG---PFEGL-KAIKDLSLGKLDCSYSNMFINHAVNYYMGESVTLS 303 Query: 293 DGRVGEIVYLNPHDIETPLVKVDDEYIDLSKLNTIKIKEI 332 D R +I+ L+ ++ PL+ DD ++DL + +K + Sbjct: 304 DNRSCKIIKLDTENLTKPLLLADDGFLDLKSEKDLYVKSL 343 >CAC0277 [T] COG2206 HD-GYP domain Length = 372 Score = 143 bits (360), Expect = 4e-34, Method: Compositional matrix adjust. Identities = 81/230 (35%), Positives = 132/230 (57%), Gaps = 4/230 (1%) Query: 95 IQEISRHSDIFALFDAIQGKDDYTYQHNIGVGVLSTLIGRWLNMSESDLSVLSLAATLHD 154 I+EI ++ I+ DDYTYQH++ V V+S +IG L + +L L + A LHD Sbjct: 117 IEEILSQKNVMIGLVDIKSFDDYTYQHSVNVAVISLIIGIKLKFHKYELFDLCIGALLHD 176 Query: 155 IGKLKIPSEILNKPGKLTPDEYELVKKHTILGYEMLKETTGANSHITLTALQHHERNDGR 214 +GK+ IPSEILNK G+LT +E++++K+HT+ GY+ LK + + +LQHHER G+ Sbjct: 177 VGKMFIPSEILNKKGELTEEEFKIMKEHTVRGYDYLKGLYEISVPARIISLQHHERAGGQ 236 Query: 215 GYPLGLKEEQIDAYAKIVAVADIFHAMSSRRPYHEPVPFHIIVDQMRRGSFGALDPHIVS 274 GYP G E+I ++IVAVAD++ AM+S R Y + + ++ + S D +V Sbjct: 237 GYPEGRSGERISILSRIVAVADVYDAMTSDRVYRRAIAPNEALEYIMANSGSKFDYKVVQ 296 Query: 275 VFLENIVKRTVGREVILTDGRVGEIVYLNPHDIETPLVKV----DDEYID 320 F ++++ G V L++ +G + ++ P +K+ DD +D Sbjct: 297 AFSKSVIPYPEGSIVRLSNNEIGVVTKIHEDFPLRPEIKIIYSEDDSRVD 346 >RSc2515 [T] COG2206 HD-GYP domain Length = 402 Score = 133 bits (335), Expect = 3e-31, Method: Compositional matrix adjust. Identities = 77/243 (31%), Positives = 118/243 (48%) Query: 72 LFESFRISRKVPLADIRKEVVPAIQEISRHSDIFALFDAIQGKDDYTYQHNIGVGVLSTL 131 +F R+ R + D + V + RH ++ KD YT+ H++ V L Sbjct: 114 MFAEARMGRTIEARDAQPLVEAISGSVDRHPGALVSLARLKTKDRYTFLHSVAVCALMVA 173 Query: 132 IGRWLNMSESDLSVLSLAATLHDIGKLKIPSEILNKPGKLTPDEYELVKKHTILGYEMLK 191 +GR L +S+ ++ LA LHDIGK+ IP E+LNKPG LT DE+ V H GY++L Sbjct: 174 LGRQLGLSDDEVRDAGLAGLLHDIGKIAIPPEVLNKPGALTTDEFRHVIGHPKAGYDILV 233 Query: 192 ETTGANSHITLTALQHHERNDGRGYPLGLKEEQIDAYAKIVAVADIFHAMSSRRPYHEPV 251 L HHER DG GYP L E I +A++ A+ D++ A++S RPY + Sbjct: 234 GDPSMRDIALDVCLHHHERMDGTGYPHKLAGENISLFARMGAICDVYDAVTSDRPYKKAW 293 Query: 252 PFHIIVDQMRRGSFGALDPHIVSVFLENIVKRTVGREVILTDGRVGEIVYLNPHDIETPL 311 + +M DP + F++++ G V L GR+ ++ + +P Sbjct: 294 EAAYAIQRMVEWRGNHFDPMVFQAFVKSVGIYPTGSLVRLRSGRLAVVMEHASGALLSPT 353 Query: 312 VKV 314 VKV Sbjct: 354 VKV 356 Database: COG Posted date: Aug 3, 2010 9:58 AM Number of letters in database: 38,378,431 Number of sequences in database: 115,334 Lambda K H 0.318 0.138 0.390 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 115334 Number of Hits to DB: 25,647,340 Number of extensions: 1126607 Number of successful extensions: 1941 Number of sequences better than 1.0e-05: 41 Number of HSP's gapped: 1908 Number of HSP's successfully gapped: 41 Length of query: 335 Length of database: 38,378,431 Length adjustment: 107 Effective length of query: 228 Effective length of database: 26,037,693 Effective search space: 5936594004 Effective search space used: 5936594004 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.4 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.7 bits) S2: 116 (49.3 bits)