BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0020.0010.p (321 letters) Database: COG 115,334 sequences; 38,378,431 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value Cgl2407 [G] COG1175 ABC-type sugar transport systems, permease c... 186 5e-47 MYPU_1010 [G] COG1175 ABC-type sugar transport systems, permease... 172 5e-43 SP1896 [G] COG1175 ABC-type sugar transport systems, permease co... 153 4e-37 BS_amyD [G] COG1175 ABC-type sugar transport systems, permease c... 144 3e-34 AGc4553 [G] COG1175 ABC-type sugar transport systems, permease c... 125 1e-28 >Cgl2407 [G] COG1175 ABC-type sugar transport systems, permease components Length = 281 Score = 186 bits (472), Expect = 5e-47, Method: Compositional matrix adjust. Identities = 114/283 (40%), Positives = 162/283 (57%), Gaps = 14/283 (4%) Query: 41 IFLWPALLFFLTIVVTPFILGFYYSSTNWNGLDLDKAVWTGADNWKRIFTNDDKFWDSLF 100 +F+ P LL F+ + PFI+GF+ S T + + A W G DN+ + F+ + F + Sbjct: 11 VFVLPTLLAFMIAFLVPFIVGFFLSFTKFT--TITNAKWVGIDNYVKAFSQREGFISAFG 68 Query: 101 FTLRFTVVSVIXXXXXXXXXXXXXXXXXKTKKVLRTIFFMPNVIGGILLGYIWQFIFTKG 160 FT+ +VSVI + RT+FFMPN+IGGI+LGY WQ + Sbjct: 69 FTVLVVIVSVITVNIFAFLLAWLLTRKLRGTNFFRTVFFMPNLIGGIVLGYTWQTMINAV 128 Query: 161 FATIGEMTGISFFQLPWLGTAGTGFWGLVIVFVWQTAGYMMVIYIAGLAGIPKDLIEAAK 220 + T IS W G+ GL+++ WQ GYMM+IYIAGL +P +LIEAA+ Sbjct: 129 LSHYA--TTIS---ADW----KFGYAGLIMLLNWQLIGYMMIIYIAGLQNVPPELIEAAE 179 Query: 221 IDGARAPQLFKSVYVPLIMPSITICLFLTTSNAFKMFDLNLSLTKGGPGTSTQSLAYNIY 280 +DG ++ + V +P++MPSITICLFLT SN+FK+FD NL+LT G PG T+ +A NI Sbjct: 180 LDGVNKWEMLRHVTIPMVMPSITICLFLTLSNSFKLFDQNLALTNGAPGGQTEMVALNII 239 Query: 281 AEALIN--NRYGLGTAKALLFFFAVSLITVTQVWLTKRKEVSA 321 L N N G+G AKA++F V +I Q+ T+ KE+ A Sbjct: 240 -NTLFNRMNVEGVGQAKAVIFVVVVVVIAYFQLRATRSKEIEA 281 >MYPU_1010 [G] COG1175 ABC-type sugar transport systems, permease components Length = 283 Score = 172 bits (437), Expect = 5e-43, Method: Compositional matrix adjust. Identities = 109/280 (38%), Positives = 159/280 (56%), Gaps = 14/280 (5%) Query: 45 PALLFFLTIVVTPFILGFYYSSTNWNGLDLD-KAVWTGADNWKRIFTNDDKFWDSLFFTL 103 PA++ F +++ P ILGF S T+W L + W G +N+K I D +F S+++T Sbjct: 13 PAIIIFSIVLIIPIILGFLLSFTDWFKEQLIFEGKWIGFNNYK-IAITDPRFIHSIWYTS 71 Query: 104 RFTVVSVIXXXXXXXXXXXXXXXXXKTKKVLRTIFFMPNVIGGILLGYIWQFIFTKGFAT 163 FT++S+I + R +FF+PN+IGG++LGY+WQ IF ++ Sbjct: 72 VFTLLSLILTNSFGFGFAYALSKKIAGTNIFRGVFFLPNMIGGLILGYLWQLIFDNALSS 131 Query: 164 IGEMTGISFFQLPWLGTAGTGFWGLVIVFVWQTAGYMMVIYIAGLAGIPKDLIEAAKIDG 223 I F G + +V+ WQ AGY+MVIYIA L + K L E+AKI+G Sbjct: 132 I-------FSHSLRFGNKWEAMLAMSMVYTWQMAGYIMVIYIAALQNVNKSLSESAKIEG 184 Query: 224 ARAPQLFKSVYVPLIMPSITICLFLTTSNAFKMFDLNLSLTKGGPGTSTQSLAYNIYAEA 283 + F SV +P IMP+ITI LFL S +F+MFD NL+LTKG T +AYNIYA A Sbjct: 185 CGVFKHFFSVILPSIMPAITIVLFLLVSRSFQMFDQNLALTKGDGQTGL--IAYNIYASA 242 Query: 284 LIN---NRYGLGTAKALLFFFAVSLITVTQVWLTKRKEVS 320 ++ +G+ AK+ +F V+ I+VTQV++TK+ EV Sbjct: 243 FVSAYQESFGIAQAKSFIFVVIVAAISVTQVYITKKFEVQ 282 >SP1896 [G] COG1175 ABC-type sugar transport systems, permease components Length = 296 Score = 153 bits (386), Expect = 4e-37, Method: Compositional matrix adjust. Identities = 93/301 (30%), Positives = 153/301 (50%), Gaps = 6/301 (1%) Query: 19 YIKRRLHMRKALRLQRGWGQQIIFLWPALLFFLTIVVTPFILGFYYSSTNWNGLDLDKAV 78 +I+ R M+K L+ W +I L +FF P G +YS TNW GL + Sbjct: 2 WIRSRETMKKVLQKYWAWAFVVIPLLLQAIFFYV----PMFQGAFYSFTNWTGLTYNYK- 56 Query: 79 WTGADNWKRIFTNDDKFWDSLFFTLRFTVVSVIXXXXXXXXXXXXXXXXXKTKKVLRTIF 138 + G +N+K +F D KF +++ FT + V+ K + R F Sbjct: 57 FVGLNNFKLLFM-DPKFMNAIGFTAIIAIAMVVGEIALGIFIARVLNSKIKGQTFFRAWF 115 Query: 139 FMPNVIGGILLGYIWQFIFTKGFATIGEMTGISFFQLPWLGTAGTGFWGLVIVFVWQTAG 198 F P V+ G+ + I++ +F G IG I FFQ LGT + V V +WQ Sbjct: 116 FFPAVLSGLTVALIFKQVFNYGLPAIGNALHIEFFQTSLLGTKWGAIFAAVFVLLWQGVA 175 Query: 199 YMMVIYIAGLAGIPKDLIEAAKIDGARAPQLFKSVYVPLIMPSITICLFLTTSNAFKMFD 258 ++I++AGL IP ++ EAA+IDGA + Q+F ++ +P ++PS+++ L FD Sbjct: 176 MPIIIFLAGLQSIPTEITEAARIDGATSKQVFWNIELPYLLPSVSMVFILALKGGLTAFD 235 Query: 259 LNLSLTKGGPGTSTQSLAYNIYAEALINNRYGLGTAKALLFFFAVSLITVTQVWLTKRKE 318 ++T GGP +T SL +Y A NN++G A A++ FF + +I++ Q+ ++K+ E Sbjct: 236 QVFAMTGGGPNNATTSLGLLVYNYAFKNNQFGYANAIAVILFFLIVVISIIQLRVSKKFE 295 Query: 319 V 319 + Sbjct: 296 I 296 >BS_amyD [G] COG1175 ABC-type sugar transport systems, permease components Length = 303 Score = 144 bits (362), Expect = 3e-34, Method: Compositional matrix adjust. Identities = 95/304 (31%), Positives = 153/304 (50%), Gaps = 9/304 (2%) Query: 20 IKRRLHMR--KALRLQRGWGQQIIFLWPALLFFLTIVVTPFILGFYYSSTNWNGLDLDKA 77 I R +H++ K + W I PALL L ++ PF+ G + TNWNG Sbjct: 4 IARDVHVKQVKPKKQSSLWWMYI----PALLSVLVFMIYPFVKGTLITFTNWNGFS-QVY 58 Query: 78 VWTGADNWKRIFTNDDKFWDSLFFTLRFTVVSVIXXXXXXXXXXXXXXXXXKTKKVLRTI 137 W G ++R+F++ D W L TL + + S KTK V RTI Sbjct: 59 QWVGFAQYERLFSDPDT-WHILKNTLHYGLGSTFFQNVVGLLYALLLNQSIKTKAVTRTI 117 Query: 138 FFMPNVIGGILLGYIWQFIFTKGFATIGEMTGI-SFFQLPWLGTAGTGFWGLVIVFVWQT 196 ++P +I +++GYIW F F+ + ++ G+ + L + W +V++ +Q Sbjct: 118 VYLPVMISPLIMGYIWYFFFSYDGGALNDLLGVFGISPINALASPSLNPWIIVMINTYQY 177 Query: 197 AGYMMVIYIAGLAGIPKDLIEAAKIDGARAPQLFKSVYVPLIMPSITICLFLTTSNAFKM 256 G MV+Y+AGL IPKD EAA++DGA+ Q F ++ +PL+MPSITI + + K+ Sbjct: 178 VGIAMVVYLAGLQSIPKDYYEAAQMDGAKQGQQFFTITLPLLMPSITINIVINIIGGLKL 237 Query: 257 FDLNLSLTKGGPGTSTQSLAYNIYAEALINNRYGLGTAKALLFFFAVSLITVTQVWLTKR 316 FD+ ++LT GGPG ++QS++ +Y G + + F + +I+ + KR Sbjct: 238 FDVIIALTAGGPGNASQSMSTFMYDLYFKRQDAGYAATQGIFMAFVILIISFCALAYFKR 297 Query: 317 KEVS 320 KE Sbjct: 298 KETE 301 >AGc4553 [G] COG1175 ABC-type sugar transport systems, permease components Length = 312 Score = 125 bits (313), Expect = 1e-28, Method: Compositional matrix adjust. Identities = 78/278 (28%), Positives = 146/278 (52%), Gaps = 4/278 (1%) Query: 42 FLWPALLFFLTIVVTPFILGFYYSSTNWNGLDLDKAVWTGADNWKRIFTNDDKFWDSLFF 101 F+ PAL+ ++V P++ + S W L +++ + G DN+ R+ +D +FW+SL+ Sbjct: 31 FVIPALVVISAVIVFPWVFTLWMSVHRWT-LGQEQS-FIGFDNYIRL-ASDLRFWESLWH 87 Query: 102 TLRFTVVSVIXXXXXXXXXXXXXXXXXKTKKVLRTIFFMPNVIGGILLGYIWQFIFTKGF 161 TL +TV+SV+ + LR +F MP + + + +W +F Sbjct: 88 TLIYTVLSVVAPLFLGTLAALVFDAQFPLRGFLRGVFVMPMMATPVAIALVWTMMFHPQL 147 Query: 162 ATIGEMTG-ISFFQLPWLGTAGTGFWGLVIVFVWQTAGYMMVIYIAGLAGIPKDLIEAAK 220 + + I L W+ T LV+V WQ +M+I + GLA +P++ E+A+ Sbjct: 148 GVLNYLLSLIGIGPLEWIYNQSTVIPSLVLVETWQWTPLVMLIVLGGLAAVPREPYESAE 207 Query: 221 IDGARAPQLFKSVYVPLIMPSITICLFLTTSNAFKMFDLNLSLTKGGPGTSTQSLAYNIY 280 IDGA A Q F+ + +P+I P + I + + + +A K FD+ ++T+GGPGT+++++ +Y Sbjct: 208 IDGANAWQKFRYLTMPMIAPFLMIAVIIRSIDAVKSFDIIYAMTQGGPGTASETINIYLY 267 Query: 281 AEALINNRYGLGTAKALLFFFAVSLITVTQVWLTKRKE 318 A G G+A A++FF + ++ + + +R + Sbjct: 268 NTAFSYYDIGYGSAMAVVFFVIIVALSFVLLMVRQRSQ 305 Database: COG Posted date: Aug 3, 2010 9:58 AM Number of letters in database: 38,378,431 Number of sequences in database: 115,334 Lambda K H 0.328 0.143 0.453 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 115334 Number of Hits to DB: 21,929,677 Number of extensions: 833287 Number of successful extensions: 3164 Number of sequences better than 1.0e-05: 155 Number of HSP's gapped: 3015 Number of HSP's successfully gapped: 155 Length of query: 321 Length of database: 38,378,431 Length adjustment: 106 Effective length of query: 215 Effective length of database: 26,153,027 Effective search space: 5622900805 Effective search space used: 5622900805 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 15 ( 7.1 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 40 (21.8 bits) S2: 116 (49.3 bits)