BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0020.0007.p (431 letters) Database: COG 115,334 sequences; 38,378,431 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value BS_ydaR [P] COG1914 Mn2+ and Fe2+ transporters of the NRAMP family 539 e-153 CAP0063 [P] COG1914 Mn2+ and Fe2+ transporters of the NRAMP family 306 3e-83 AGc3186 [P] COG1914 Mn2+ and Fe2+ transporters of the NRAMP family 261 2e-69 ML2098 [P] COG1914 Mn2+ and Fe2+ transporters of the NRAMP family 244 2e-64 RSp0520 [P] COG1914 Mn2+ and Fe2+ transporters of the NRAMP family 240 3e-63 >BS_ydaR [P] COG1914 Mn2+ and Fe2+ transporters of the NRAMP family Length = 158 Score = 539 bits (1388), Expect = e-153, Method: Compositional matrix adjust. Identities = 0/430 (0%), Positives = 0/430 (0%), Gaps = 6/430 (1%) Query: 1 MMEQNLAETPPMIHRQKNKLTAQAVLNGDVKGLKRLLPFLGPAFIASVAYLDPGNFATNI 60 M+ Q + + +G VK + + ++ G+ + + Sbjct: 1 MLSGKDLGRAI---EQA---INKKIASGSVKSKAEVARHFKVQPPSIYDWIKKGSISKDK 54 Query: 61 TAGSKYGYLLLWVIFASNLMAVLIQSLSAKLGIATGKNLPEVARERFPKGVSIFLWIQSE 120 + + L I + + + ++ + + + +I ++ S Sbjct: 55 LPELWRFFSDVVGPEHWGLNEYPIPTPTNSDTKSELLDINNLYQAASDEIRAIVAFLLSG 114 Query: 121 LVIIATDLAEFIGAALGLYLLFGIPMLPAALITAVGSFAILELQRRGYRTLEAGIAGMVL 180 Sbjct: 115 174 Query: 181 IVVLAFTFQVIMAKPDAGAVAAGIFTPQFQGVDSILLAAGILGATVMPHAIYLHSSLTQS 240 Sbjct: 175 234 Query: 241 RVVGIDDKEKKQIFKLEFIDILIAMLIXXXXXXXXXXXXXXLFFKNGLVVEDLDIAFEQF 300 Sbjct: 235 294 Query: 301 RHLAGPVTAVSFXXXXXXXXXXXXXXXTMAGDVVMQGFINKRINLYLRRXXXXXXXXXXX 360 Sbjct: 295 354 Query: 361 XFGINATSALVMSQVVLSFGIAFALIPLVIFTSDRKIMNGLVNHRITTVLGWIISALVVS 420 Sbjct: 355 414 Query: 421 LNLFLIVEMF 430 Sbjct: 415 424 >CAP0063 [P] COG1914 Mn2+ and Fe2+ transporters of the NRAMP family Length = 431 Score = 306 bits (785), Expect = 3e-83, Method: Compositional matrix adjust. Identities = 164/387 (42%), Positives = 232/387 (59%) Query: 31 KGLKRLLPFLGPAFIASVAYLDPGNFATNITAGSKYGYLLLWVIFASNLMAVLIQSLSAK 90 + LK LL FLGPAF+ SVAY+DPGNFATNI+ GS + Y L+WVI SNLMA+ +Q++SAK Sbjct: 28 RNLKGLLKFLGPAFVVSVAYIDPGNFATNISGGSSFNYNLIWVILWSNLMAIFLQTMSAK 87 Query: 91 LGIATGKNLPEVARERFPKGVSIFLWIQSELVIIATDLAEFIGAALGLYLLFGIPMLPAA 150 LGIATG +LPE+ + F K + WI EL +ATDLAEFIG LGLYLLF IPM+ A Sbjct: 88 LGIATGCSLPEMCAKVFSKRANWIFWIVGELGAMATDLAEFIGGTLGLYLLFRIPMIYAG 147 Query: 151 LITAVGSFAILELQRRGYRTLEAGIAGMVLIVVLAFTFQVIMAKPDAGAVAAGIFTPQFQ 210 L+T V +F I+ +++ G + +E IA ++ ++ +A+T ++ +A+P V P Sbjct: 148 LLTGVLTFIIVYMEKYGQKMVETIIAALIAVICVAYTIELFLARPAWTQVGMHTLIPSLP 207 Query: 211 GVDSILLAAGILGATVMPHAIYLHSSLTQSRVVGIDDKEKKQIFKLEFIDILIAMLIXXX 270 +++L+A G+LGATVMPH IYLHS L Q R DKEK K+E IDILIAM I Sbjct: 208 NGEAVLIAVGMLGATVMPHVIYLHSELVQHRNTNSSDKEKLHHLKMEKIDILIAMNIAFV 267 Query: 271 XXXXXXXXXXXLFFKNGLVVEDLDIAFEQFRHLAGPVTAVSFXXXXXXXXXXXXXXXTMA 330 +FFK+G+ V ++ A + L G +++ +F TMA Sbjct: 268 VNAAMVIVSAAVFFKHGIKVSTIEEAHRSLQPLLGNLSSGAFGIALLASGLSSSAVGTMA 327 Query: 331 GDVVMQGFINKRINLYLRRXXXXXXXXXXXXFGINATSALVMSQVVLSFGIAFALIPLVI 390 G +M+GF+N I + LRR GIN LV+SQV LSF + F +I +++ Sbjct: 328 GQTIMKGFVNLSIPINLRRIITMLPALIIIALGINPMRVLVLSQVALSFILPFPIIQMLL 387 Query: 391 FTSDRKIMNGLVNHRITTVLGWIISAL 417 + +M LVN + T ++G+II+ + Sbjct: 388 IAGRKDLMGILVNKKFTKIVGFIIATM 414 >AGc3186 [P] COG1914 Mn2+ and Fe2+ transporters of the NRAMP family Length = 289 Score = 261 bits (666), Expect = 2e-69, Method: Compositional matrix adjust. Identities = 0/411 (0%), Positives = 0/411 (0%), Gaps = 12/411 (2%) Query: 29 DVKGLKRLLPFLGPAFIASVAYLDPGNFATNITAGSKYGYLLLWVIFASNLMAVLIQSLS 88 A A + + ++ Sbjct: 49 VSPDDFPKFIATLKEGKPGAAVGGNATIPHKEAAYRLADHPDALAEELGAANTIWMEEGK 108 Query: 89 AKLGIATGKNLPEVARERFPKGVSIFLWIQSELVIIATDLAEFIGAALGLYLLFGIPMLP 148 G ER P + + + + + + + Sbjct: 109 LHATNTDGYGFVSNLDERHPGWDKTQRAVVFGAGGASRAVIQSLRDRDVAEIHVVNRTVE 168 Query: 149 AALITAVGSFAILELQRRGYRTLEAGIAGMVLIVVLAFTFQVIMAKPDAGAVAAGIFTPQ 208 A A + + AG+ + D +AA Sbjct: 169 RARELADRFGPRVFSHPQAALQEVMHGAGLFVNTTSLGMNGTEAPHLDFSHLAAN-AVVT 227 Query: 209 FQGV---DSILLAAGILGATVMPHAIYLHSSLTQSRVVGIDDKEKKQIFKLEFIDILIAM 265 + +L + + + + + L + I Sbjct: 228 DIVYVPLKTPILNMAQEQGIATVDGLGMLLHQAKPGFRRWFGRIPEVDETLRSLIIADME 287 Query: 266 LIXXXXXXXXXXXXXXLFFKNGLV-VEDLDIAFEQFRHLAGPVTAVS-FXXXXXXXXXXX 323 Sbjct: 288 347 Query: 324 XXXXTMAGDVVMQGFINKRINLYLRRXXXXXXXXXXXX-----FGINATSA-LVMSQVVL 377 Sbjct: 348 407 Query: 378 SFGIAFALIPLVIFTSDRKIMNGLVNHRITTVLGWIISALVVSLNLFLIVE 428 Sbjct: 408 458 >ML2098 [P] COG1914 Mn2+ and Fe2+ transporters of the NRAMP family Length = 311 Score = 244 bits (623), Expect = 2e-64, Method: Compositional matrix adjust. Identities = 0/413 (0%), Positives = 0/413 (0%), Gaps = 4/413 (0%) Query: 16 QKNKLTAQAVLNGDVKGLKRLLPFLGPAFIASVAYLDPGNFATNITAGSKYGYLLLWVIF 75 ++ + V + + L + L+ + I + + + Sbjct: 10 LESLKNLKRNGWMTVAAVSSVTITLVLVGLFLSVILNTSKISNGIENNVRIVAYMKLDVH 69 Query: 76 ASNLMAVLIQSLSAKLGIATGKNLPEVARERFPKGVSIFLWIQSELVIIATDLAEFIGAA 135 + + + + ++ F +++L + L Sbjct: 70 DQETKIQDPKDNKKTINNPDHLKIYDSIKKVPNVKTVEFSSKEAQLKELTKALGNNWNLF 129 Query: 136 LGLYLLFGIPMLPAALITAVGSFAILELQ-RRGYRTLEAGIAGMVLIVVLAFTFQVIMAK 194 G + A A ++ G G A + +A + Sbjct: 130 KGDANPLYDAYIVEADKPANVKSVTSKISKLSGIEKATYGGANTAKLFQIAQGIRTWGTA 189 Query: 195 PDAGAVAAGIFTPQFQGVDSILLAAGILGATVMPHAIYLHSSLTQSRVVGID-DKEKKQI 253 + +F + +L+ + + G I Sbjct: 190 AAILLIFVAVF-LISNTIRITILSRQREIQIMRLVGAKNGYIRWPFFLEGAWVGLLGSII 248 Query: 254 FKLEFIDILIAMLIXXXXXXXXXXXXXXLFFKNGLVVEDLDIAFEQFRHLAGPVTAVSFX 313 L + K + +A + Sbjct: 249 PGLIVAWAYRLAFV-SLTPSLQSQKLAMFAPKEFIPAIVGLMAVIGILIGSFGALLSMRR 307 Query: 314 XXXXXXXXXXXXXXTMAGDVVMQGFINKRINLYLRRXXXXXXXXXXXXFGINATSALVMS 373 Sbjct: 308 367 Query: 374 QVVLSFGIAFALIPLVIFTSDRKIMNGLVNHRITTVLGWIISALVVSLNLFLI 426 Sbjct: 368 420 >RSp0520 [P] COG1914 Mn2+ and Fe2+ transporters of the NRAMP family Length = 442 Score = 240 bits (613), Expect = 3e-63, Method: Compositional matrix adjust. Identities = 146/413 (35%), Positives = 229/413 (55%), Gaps = 12/413 (2%) Query: 29 DVKGLKRLLPFLGPAFIASVAYLDPGNFATNITAGSKYGYLLLWVIFASNLMAVLIQSLS 88 D+ K+LL F GP + SV Y+DPGN+AT+I AGS+YGY LL+V+ S+L A+++Q LS Sbjct: 29 DLPLWKKLLRFAGPGLLVSVGYMDPGNWATDIEAGSRYGYSLLFVVLLSSLAAMVLQCLS 88 Query: 89 AKLGIATGKNLPEVARERFPKGVSIFLWIQSELVIIATDLAEFIGAALGLYLLFGIPMLP 148 A+LGI TGK+L ++RER+ G W+ +EL I+A DLAE +G AL +LL G+P+L Sbjct: 89 ARLGIVTGKDLARLSRERYRPGAVRVQWLLAELSIVACDLAEVLGCALAFHLLLGVPILG 148 Query: 149 AALITAVGSFAILELQRRGYRTLEAGIAGMVLIVVLAFTFQVIMAKPDAGAVAAGIFTPQ 208 +TA+ + +L L+ + +R LEA + G++L + L + ++ + +P +V AG P Sbjct: 149 GVALTALDTLIVLGLKGKNFRQLEAIVLGLILTIGLCYFVELALIRPHWPSV-AGALVPS 207 Query: 209 FQGV---DSILLAAGILGATVMPHAIYLHSSLTQSRVVGIDDKEKKQIFKLEFIDILIAM 265 +Q + + + LA GILGATVMPH +YLHSS+ Q+RVV + +++ L +D ++++ Sbjct: 208 WQALSAREPLYLAIGILGATVMPHNLYLHSSIVQTRVVSETEPARREAVGLSRLDTIVSL 267 Query: 266 LIXXXXXXXXXXXXXXLFFKNGLV-VEDLDIAFEQFRHLAG-PVTAVSFXXXXXXXXXXX 323 + F NG V D+ A + G V V F Sbjct: 268 SLALLVNGAILVLAAAAFHANGHQDVADIQDAHRLLEPIVGTAVAGVLFGIALLAAGQSS 327 Query: 324 XXXXTMAGDVVMQGFINKRINLYLRRXXXXXXXXXXXXFGINATSA------LVMSQVVL 377 T+AG ++M+GF+ RI + RR G+ LV+SQVVL Sbjct: 328 TFTGTIAGQILMEGFLELRIPCWQRRLATRALALIPAFVGVAMLGDHAIGRLLVISQVVL 387 Query: 378 SFGIAFALIPLVIFTSDRKIMNGLVNHRITTVLGWIISALVVSLNLFLIVEMF 430 F + FA+ PL+ T DR +M N R+T+ + W + A++ NL+L+ ++ Sbjct: 388 GFQLPFAMFPLIRMTGDRALMGTFANGRLTSAVAWCLFAVISVANLWLVWQVL 440 Database: COG Posted date: Aug 3, 2010 9:58 AM Number of letters in database: 38,378,431 Number of sequences in database: 115,334 Lambda K H 0.328 0.142 0.406 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 115334 Number of Hits to DB: 26,112,723 Number of extensions: 921248 Number of successful extensions: 2838 Number of sequences better than 1.0e-05: 19 Number of HSP's gapped: 2808 Number of HSP's successfully gapped: 19 Length of query: 431 Length of database: 38,378,431 Length adjustment: 109 Effective length of query: 322 Effective length of database: 25,807,025 Effective search space: 8309862050 Effective search space used: 8309862050 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 15 ( 7.1 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 40 (21.7 bits) S2: 117 (49.7 bits)