BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0020.0001.p (355 letters) Database: COG 115,334 sequences; 38,378,431 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value SMb21003 [C] COG2141 Coenzyme F420-dependent N5,N10-methylene te... 353 3e-97 AGc4197 [C] COG2141 Coenzyme F420-dependent N5,N10-methylene tet... 338 6e-93 Cgl2071 [C] COG2141 Coenzyme F420-dependent N5,N10-methylene tet... 238 1e-62 SA0327 [C] COG2141 Coenzyme F420-dependent N5,N10-methylene tetr... 207 2e-53 Cgl1604 [C] COG2141 Coenzyme F420-dependent N5,N10-methylene tet... 99 1e-20 >SMb21003 [C] COG2141 Coenzyme F420-dependent N5,N10-methylene tetrahydromethanopterin reductase and related flavin-dependent oxidoreductases Length = 346 Score = 353 bits (906), Expect = 3e-97, Method: Compositional matrix adjust. Identities = 19/345 (5%), Positives = 59/345 (17%), Gaps = 2/345 (0%) Query: 8 MEIGISTFLETTPDPVTGEVISHAERLREAVEEIVLADQVGLDVYGIGEHHRIDYXXXXX 67 M+ G + D + + + Sbjct: 1 MQYHRIPHSSLEVS-TLGLGTMTFGEQNSEADAHAQLDYAVAQGINLIDVAEMYPVPPRP 59 Query: 68 XXXXXXXXXMTKNIRLTSAVTVLSSDDPVRVYQAFSTLDGLSGGRAEIMAGRGSFIESFP 127 + + + L V + + R + +S Sbjct: 60 ETQGLTETYVGNWLAKHGSREKLIIASKVSGPSRNNDKGIRPDQALDRKNIREALHDSLK 119 Query: 128 LFGYSXXXXXXXXXXXXXXXXKIRASEKVTWRGGHRPAINNLPVYPRAVQDPLPVWIASG 187 + T ++ L + +I Sbjct: 120 RLQTDYLDLYQVHWPQRPTNCFGKLGYSWTDSAPAVSLLDTLDALAEYQRAGKIRYIGVS 179 Query: 188 GNPESAIRAGMLGLPIAFAIIGGMPERFAPLVQLYKDAAAKAGHNPDKLQIATHSHGFVG 247 + + + L+ + + +++ +S G Sbjct: 180 NETAFGVMRYLHLADKHDLPRIVTIQNPYSLLNRSFEVGLAEVSQYEGVELLAYSCLGFG 239 Query: 248 ETTEQAAALFYPSTMAQMNVIGRERGWGQAYNRATYDDARSLRGALYVGDAEYVAEKILL 307 T + P+ R G+ + + + A+ + Sbjct: 240 TLTGKYLNGAKPAGARNTLFSRFTRYSGEQ-TQKAVAAYVDIARRHGLDPAQMALAFVRR 298 Query: 308 LRKNLGVTRFFLHVNVGTMPHREVLRAIELLGTKVAPIVRRELAK 352 ++ + + V + Sbjct: 299 QPFVASTLLGATTMDQLKTNIESLHLELSEDVLAEIEAVHQVYTY 343 >AGc4197 [C] COG2141 Coenzyme F420-dependent N5,N10-methylene tetrahydromethanopterin reductase and related flavin-dependent oxidoreductases Length = 346 Score = 338 bits (868), Expect = 6e-93, Method: Compositional matrix adjust. Identities = 26/347 (7%), Positives = 59/347 (17%), Gaps = 1/347 (0%) Query: 8 MEIGISTFLETTPDPVTGEVISHAERLREAVEEIVLADQVGLDVYGIGEHHRIDYXXXXX 67 M+ + ++ E+ EA L V + I Sbjct: 1 MQYHRIPHSSLEVSTLGLGTMTFGEQNSEADAHAQLDYAVAQGINLIDVAEMYPVPPRPE 60 Query: 68 XXXXXXXXXMTKNIRLTSAVTVLSSDDPVRVYQAFSTLDGLSGGRAEIMAGRGSFIESFP 127 + S ++ + + Sbjct: 61 TQGLTETYVGNWLAKHGSREKLIIASKVSGPSRNNDKGIRPDQALDRKNIREALHDSLKR 120 Query: 128 LFGYSXXXXXXXXXXXXXXXXKIRASEKVTWRGGHRPAINNLPVYPRAVQDPLPVWIASG 187 L + + S Sbjct: 121 LQTDYLDLYQVHWPQRPTNCFGKLGYSWTDSAPAVSLLDTLDALAEYQRAGKIRYIGVSN 180 Query: 188 GNPESAIRAGMLGLPIAFAIIGGMPERFAPLVQLYKDAAAKAGHNPDKLQIATHSHGFVG 247 +R L I + ++ L + ++ A+ +A GF Sbjct: 181 ETAFGVMRYLHLADKHDLPRIVTIQNPYSLLNRSFEVGLAEVSQYEGVELLAYSCLGFGT 240 Query: 248 ETTEQAAALFYPSTMAQMNVIGRERGWGQAYNRATYDDARSLRGALYVGDAEYVAEKILL 307 T + + Q +A R +A Sbjct: 241 LTGKYLNGAKPAGARNTLFSRFTRYSGEQT-QKAVAAYVDIARRHGLDPAQMALAFVRRQ 299 Query: 308 LRKNLGVTRFFLHVNVGTMPHREVLRAIELLGTKVAPIVRRELAKGA 354 + + T L E + ++ + + Sbjct: 300 PFVASTLLGATTMDQLKTNIESLHLELSEDVLAEIEAVHQVYTYPAP 346 >Cgl2071 [C] COG2141 Coenzyme F420-dependent N5,N10-methylene tetrahydromethanopterin reductase and related flavin-dependent oxidoreductases Length = 346 Score = 238 bits (606), Expect = 1e-62, Method: Compositional matrix adjust. Identities = 0/353 (0%), Positives = 0/353 (0%), Gaps = 10/353 (2%) Query: 6 NSMEIGISTFLETTPDPVTGEVISHAERLREAVEEIVLADQVGLDVYGIGEHHRIDYXXX 65 I S+ +T + +A ++ A G+++ + E + + Sbjct: 2 QYHRIPHSSLEVSTLG-LGTMTFGEQNSEADAHAQLDYAVAQGINLIDVAEMYPVPPRPE 60 Query: 66 XXXXXXXXXXXMTKNIRLTSAVTVLSSDDPVRVYQAFSTLDGLSGGRAEIMAGRGSFIES 125 + + S + R I ++ Sbjct: 61 TQGLTETYVGNWLAKHGSREKLIIASKVSGPSRNNDKGIRPDQALDRKNIREALHDSLKR 120 Query: 126 FPLFGYSXXXXXXXXXXXXXXXXKIRASEK----VTWRGGHRPAINNLPVYPRAVQDPLP 181 + V+ + Sbjct: 121 LQTDYLDLYQVHWPQRPTNCFGKLGYSWTDSAPAVSLLDTLDALAEYQRAGK-IRYIGVS 179 Query: 182 VWIASGGNPESAIRAGMLGLPIAFAIIGGMPERFAPLVQLYKDAAAKAGHNPDKLQIATH 241 A G + I + V L + + + Sbjct: 180 NETAFGVMRYLHLADKHDLPRIVTIQNPYSLLNRSFEVGLAEVSQYEGVE-LLA-YSCLG 237 Query: 242 SHGFVGETTEQAAALFYPSTMAQMNVIGRERGWGQAYNRATYDDARSLRGALYVGDAEYV 301 G+ A +T+ +A A Y D G A Sbjct: 238 FGTLTGKYLNGAKPAGARNTLFSRFTRYSGEQTQKA--VAAYVDIARRHGLDPAQMALAF 295 Query: 302 AEKILLLRKNLGVTRFFLHVNVGTMPHREVLRAIELLGTKVAPIVRRELAKGA 354 Sbjct: 296 348 >SA0327 [C] COG2141 Coenzyme F420-dependent N5,N10-methylene tetrahydromethanopterin reductase and related flavin-dependent oxidoreductases Length = 346 Score = 207 bits (527), Expect = 2e-53, Method: Compositional matrix adjust. Identities = 0/347 (0%), Positives = 0/347 (0%), Gaps = 7/347 (2%) Query: 8 MEIGISTFLETTPDPVTGEVISHAERLREAVEEIVLADQVGLDVYGIGEHHRIDYXXXXX 67 + T + + A ++ V E + Sbjct: 12 EVSTLGLGTMTFGEQNSEADAHAQLDYAVAQGINLIDVAEMYPVPPRPETQGLTETYVGN 71 Query: 68 XXXXXXXXXMTKNIRLTSAVTVLSSDDPVRVYQAFSTLDGLSGGRAEIMAGRGSFIESFP 127 S + + + + Sbjct: 72 WLAKHGSREKLIIASKVSGPSRNNDKGIRPDQALDRKNIREALHDSLKRLQTDYLDLYQV 131 Query: 128 LFGYSXXXXXXXXXXXXXXXXKIRASEKVTWRGGHRPAINNLPVYPRAVQDPLPVWIASG 187 + + + + + V Sbjct: 132 HWPQRPTNCFGKLGYSWTDSAPAVSLLDTLDALAEYQRAGKIRYIGVSNETAFGVMRYLH 191 Query: 188 GNPESAIRAGMLGLPIAFAIIGGMPERFAPLVQLYKDAAAKAGHNPD--KLQIATHSHGF 245 + + + + A + Q + Sbjct: 192 LADKHDLPRIVTIQNPYSLLNRSFEVGLAEVSQYEGVELLAYSCLGFGTLTGKYLNGAKP 251 Query: 246 VGETTEQAAALFYPSTMAQMNVIGRERGWGQAYNRATYDDARSLRGALYVGDAEYVAEKI 305 G + S + + + + +V +VA + Sbjct: 252 AGARNTLFSRFTRYSGEQTQKAV---AAYV--DIARRHGLDPAQMALAFVRRQPFVASTL 306 Query: 306 LLLRKNLGVTRFFLHVNVGTMPHREVLRAIELLGTKVAPIVRRELAK 352 Sbjct: 307 353 >Cgl1604 [C] COG2141 Coenzyme F420-dependent N5,N10-methylene tetrahydromethanopterin reductase and related flavin-dependent oxidoreductases Length = 346 Score = 99.0 bits (245), Expect = 1e-20, Method: Compositional matrix adjust. Identities = 20/340 (5%), Positives = 51/340 (15%), Gaps = 14/340 (4%) Query: 8 MEIGISTFLETTPDPVTGEVISHAERLREAVEEIVLADQVGLDVYGIGEHHRIDYXXXXX 67 M+ + ++ E+ EA L V + I Sbjct: 1 MQYHRIPHSSLEVSTLGLGTMTFGEQNSEADAHAQLDYAVAQGINLIDVAEMYPVPPRPE 60 Query: 68 XXXXXXXXXMTKNIRLTSAVTVLSSDDPVRVYQAFSTLDGLSGGRAEIMAGRGSFIESFP 127 + S ++ + + Sbjct: 61 TQGLTETYVGNWLAKHGSREKLIIASKVSGPSRNNDKGIRPDQALDRKNIREALHDSLKR 120 Query: 128 LFGYSXXXXXXXXXXXXXXXXKIRASEKVTWRGGHRPAINNLPVYPRAVQDPLP-VWIAS 186 L + + + +++ Sbjct: 121 LQTDYLDLYQVHWPQRPTNCFGKLGYSWTDSAPAVSLLDTLDALAEYQRAGKIRYIGVSN 180 Query: 187 GGNPESAIRAGMLGLPIAFAIIGGMPERFAPLVQLYKDAAAKAGH-NPDKLQIATHSHGF 245 + I+ A + + + L + G Sbjct: 181 ETAFGVMRYLHLADKHDLPRIVTIQNPYSLLNRSFEVGLAEVSQYEGVELLAYSCLGFGT 240 Query: 246 VGETTEQAAALFYPSTMAQMNVIGRERGWGQAYNRATYDDARSLRGALYVGDAEYVAEKI 305 + A + R G Q A + + Sbjct: 241 LTGKYLNGAKPAGAR-NTLFSRFTRYSGE-QTQKAVA---------AYVDIARRHGLDPA 289 Query: 306 LLLRKNLG-VTRFFLHVNVGTMPHREVLRAIELLGTKVAP 344 + + + T + L Sbjct: 290 QMALAFVRRQPFVASTLLGATTMDQLKTNIESLHLELSED 329 Database: COG Posted date: Aug 3, 2010 9:58 AM Number of letters in database: 38,378,431 Number of sequences in database: 115,334 Lambda K H 0.319 0.136 0.398 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 115334 Number of Hits to DB: 24,896,611 Number of extensions: 979654 Number of successful extensions: 1695 Number of sequences better than 1.0e-05: 9 Number of HSP's gapped: 1679 Number of HSP's successfully gapped: 9 Length of query: 355 Length of database: 38,378,431 Length adjustment: 107 Effective length of query: 248 Effective length of database: 26,037,693 Effective search space: 6457347864 Effective search space used: 6457347864 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.4 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.8 bits) S2: 116 (49.3 bits)