BLASTP 2.2.18 [Mar-02-2008]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Reference for compositional score matrix adjustment: Altschul, Stephen F., 
John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis,
Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches
using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109.

Query= 0019.0022.p
         (690 letters)

Database: COG 
           115,334 sequences; 38,378,431 total letters

Searching..................................................done



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

BH3812 [M] COG0744 Membrane carboxypeptidase (penicillin-binding...   550   e-156
BS_ywhE [M] COG0744 Membrane carboxypeptidase (penicillin-bindin...   485   e-137
sll1434 [M] COG0744 Membrane carboxypeptidase (penicillin-bindin...   410   e-114
alr5324 [M] COG0744 Membrane carboxypeptidase (penicillin-bindin...   381   e-105
sll0002_2 [M] COG0744 Membrane carboxypeptidase (penicillin-bind...   365   e-100

>BH3812 [M] COG0744 Membrane carboxypeptidase (penicillin-binding protein)
          Length = 687

 Score =  550 bits (1416), Expect = e-156,   Method: Compositional matrix adjust.
 Identities = 294/627 (46%), Positives = 393/627 (62%), Gaps = 20/627 (3%)

Query: 71  GRSREPVTLNQISPLLIQATLAVEDRKFYKHSGFDVKGMGRAVLANLESGDRTQGASTLT 130
           G+ R  V L++ISP L +AT+++EDRKFY+H GFD+  +G AV+ANL +G   QGAST+T
Sbjct: 71  GQQRYWVALDEISPYLKEATISIEDRKFYQHHGFDLTRIGGAVVANLRAGSMAQGASTIT 130

Query: 131 QQLARNLYLSHEKTWTRKAKEALYTMQLEMKYSKDEILNMYLNQIYYGHGAYGIEAASQM 190
           QQ ARNLYL H+KTW RKA+EALY ++LEM Y+KD+IL  YLN IYYGHGAYGIEAA+Q 
Sbjct: 131 QQYARNLYLGHDKTWNRKAQEALYALRLEMHYTKDDILEGYLNTIYYGHGAYGIEAAAQH 190

Query: 191 YFGKAAADLDLAESAILAGIPKGPTYYSPYTHMDNAKKRQRIILAAMTEVGDITEAQATA 250
           +FGK AA+L LAE+++LAG+PKGP+YYSP    D AK RQ IIL +M E G IT  +A  
Sbjct: 191 FFGKKAANLTLAEASMLAGVPKGPSYYSPLVDEDRAKARQEIILQSMVENGYITAQEAEE 250

Query: 251 ARLETLSFKPQGEKNTLAAAPYFRDYVRSLATDTLHISSEELDQGGLNVYTTLDPDMQQA 310
           A  E L +   G   +    PYF+D V     + + +    L+ GGL++YTTLDP MQ+ 
Sbjct: 251 AINEALDYVTDGGMRSETIGPYFQDVVEEQLVEEVGLDPALLESGGLHIYTTLDPHMQEE 310

Query: 311 AEDAVEKGMDPSGDLETALVSIDPRTGYIKAMVGGKNYRTNQFNHALATTRQPGSSFKPI 370
           AE  VE  M P  DL+ ALV+IDP TG ++A+VGGKNY  + FN A    R PGSSFKP 
Sbjct: 311 AERWVEVEM-PDSDLQVALVAIDPSTGDVRALVGGKNYGESSFNRATKARRSPGSSFKPF 369

Query: 371 MYLTALSSKSMTGLSVFNSQPTLFHYDNDRKTYQPKNFGDKYLGE-INMRQAIAASDNIY 429
           +Y  AL  +  T  S   S+PT F YD  R TY P+NF D+Y  + + + QA+A SDNI+
Sbjct: 370 LYYAAL-EQGFTPASTLISEPTTFSYDQGRSTYSPRNFQDRYKDDFVTLLQALAFSDNIF 428

Query: 430 AVNTIMKVGADKVADMAAKMGIDSPLQTVPSLALGTSPVSPLEMASAFAVIGGGGVKLPV 489
           AV T M +G D++ + A + GI+SPL+ +PSLALGT PV  LE+  ++      G  +  
Sbjct: 429 AVKTHMFLGTDELVETARRFGIESPLRDIPSLALGTLPVGVLELTKSYVPFANNGFTVEP 488

Query: 490 TAILKITDANGKLLYEA-PQTQGEKVVEPAAAYVLTRLMEGVFE------SGGTGNRVAS 542
             I K+ D +G +LYE  P+T  E+ ++P   +++  LM  +F+         TG+ V  
Sbjct: 489 RFITKVVDRDGNVLYEQMPET--EQTLDPRLTFIMADLMTAMFDPSLNDYMSVTGSSVNH 546

Query: 543 MIKRPVAGKTGTTDTDGWMVGFTPELSTAVWVGYDKGRDIATA-DGRRAAPIFAQFTEKA 601
           ++ RPVAGK+G+T TD WMVGFTP+L T VW+GYD+   +    +G+ A  I+A FTE A
Sbjct: 547 LVNRPVAGKSGSTSTDSWMVGFTPQLVTGVWIGYDQSETLNHGTEGQVAKRIWANFTEHA 606

Query: 602 LENVPPKIFPIPDGVVSVYVNPQSGKLATAACPEKVLETFISGTEPKEYCDQHGAGGAVS 661
           LEN     F  P GVVSV +NP +G LA   CP      F+ GTEP E C +        
Sbjct: 607 LENELMLQFRQPPGVVSVNMNPSNGFLANDVCPNGRKTFFVEGTEPTEGCPEEAR----- 661

Query: 662 PPDGSGEPPAEDIKEEHSLWKDIKRWW 688
             D   +   E+  E+        RW+
Sbjct: 662 --DNETDAIDEENHEKERFLDRFIRWF 686


>BS_ywhE [M] COG0744 Membrane carboxypeptidase (penicillin-binding protein)
          Length = 647

 Score =  485 bits (1249), Expect = e-137,   Method: Compositional matrix adjust.
 Identities = 253/575 (44%), Positives = 365/575 (63%), Gaps = 10/575 (1%)

Query: 68  TTDGRSREPVTLNQISPLLIQATLAVEDRKFYKHSGFDVKGMGRAVLANLESGDRTQGAS 127
           T  G  R  V L  ++P +++AT+A+ED+ FY H GFD K M  A LA+L++  + QGAS
Sbjct: 68  TNYGEKRYWVPLKDMNPTIVKATVAIEDQNFYDHHGFDYKRMAGAALADLKAFAKVQGAS 127

Query: 128 TLTQQLARNLYLSHEKTWTRKAKEALYTMQLEMKYSKDEILNMYLNQIYYGHGAYGIEAA 187
           T+TQQ ARNLYL H+KTW RK  EA YT++LE  YSKDEIL  YLN IYYGHGAYGIEAA
Sbjct: 128 TITQQYARNLYLEHDKTWKRKWNEAFYTIRLEQNYSKDEILEGYLNTIYYGHGAYGIEAA 187

Query: 188 SQMYFGKAAADLDLAESAILAGIPKGPTYYSPYTHMDNAKKRQRIILAAMTEVGDITEAQ 247
           S++YFGK A +L  AE+A+LAGIPKGP+ YSPY +   AK+RQ+ I+  M +   I++ +
Sbjct: 188 SRLYFGKHAKNLTDAEAALLAGIPKGPSGYSPYVNETKAKERQKTIVRMMEKQQMISQKK 247

Query: 248 ATAARLETLSFKPQGEKNTLAAAPYFRDYVRSLATDTLHISSEELDQGGLNVYTTLDPDM 307
           A     E LS++P  ++ +   APYF D         L ++ E+++  GLNVYTT+D  M
Sbjct: 248 ADELIKEPLSYQPLNKQVSKRKAPYFYDNAMRELEKKLGMTREQIETSGLNVYTTVDKRM 307

Query: 308 QQAAEDAVEKGMDPSGDLETALVSIDPRTGYIKAMVGGKNYRTNQFNHALATTRQPGSSF 367
           Q+ AE+ + + ++   D++    +IDPRTG + A+VGG++Y+ + F+      RQP S+ 
Sbjct: 308 QRIAEETITETVNAGSDIQVGFSAIDPRTGNVLALVGGRDYQKSPFDRTTQAKRQPASTI 367

Query: 368 KPIMYLTALSSKSMTGLSVFNSQPTLFHYDNDRKTYQPKNFGDKYLGE-INMRQAIAASD 426
           KP++Y  A+ S   T +++  S+ T F  D   +TY P N+   Y  + I + QA+A SD
Sbjct: 368 KPLLYYKAIQS-GFTPVTLMKSEETEFQIDAKGETYSPSNYNGYYANKPITLLQALALSD 426

Query: 427 NIYAVNTIMKVGADKVADMAAKMGIDSPLQTVPSLALGTSPVSPLEMASAFAVIGGGGVK 486
           NIYAV T + +G +K+   A + GI + LQ +PSLALGT PV P+EM +A+A++  GG K
Sbjct: 427 NIYAVKTHLFLGTNKLVKTAKEFGITAHLQALPSLALGTEPVRPIEMVNAYAMLANGGKK 486

Query: 487 LPVTAILKITDANGKLLYEAPQTQGEKVVEPAAAYVLTRLMEGVFE------SGGTGNRV 540
           +  T I ++TDA G +LYE P  Q ++V++  AA+V   +M G+F+      +  TG  +
Sbjct: 487 IEPTFISRVTDAAGHVLYENPN-QHKQVLDEKAAFVTASMMTGMFDIDLNGYTSVTGRTI 545

Query: 541 ASMIKRPVAGKTGTTDTDGWMVGFTPELSTAVWVGYDKGRDIATADGRR-AAPIFAQFTE 599
           A+ + R  AGK+GTT  D WM+GF P+L+  VW GYDK   I + + +  A  I+A F E
Sbjct: 546 ANRLTRTYAGKSGTTSADSWMIGFNPKLAAGVWTGYDKNSTIDSVEEKSYAKTIWADFME 605

Query: 600 KALENVPPKIFPIPDGVVSVYVNPQSGKLATAACP 634
            AL+  P   F  P GV  VY++P++G  +  A P
Sbjct: 606 DALKGEPETAFKPPKGVTGVYIDPETGYSSGPAAP 640


>sll1434 [M] COG0744 Membrane carboxypeptidase (penicillin-binding protein)
          Length = 650

 Score =  410 bits (1055), Expect = e-114,   Method: Compositional matrix adjust.
 Identities = 239/575 (41%), Positives = 352/575 (61%), Gaps = 27/575 (4%)

Query: 54  SKLLDSRGNVLTTFTTDGRSREPVTLNQISPLLIQATLAVEDRKFYKHSGFDVKGMGRAV 113
           S + D++G  L +   +   R+ V L +ISP L +A LA+ED  FYKH G +   +GRA+
Sbjct: 74  SYIYDAKGRELLSLHGE-EHRKIVPLAEISPNLKRAVLAIEDSNFYKHQGINPNSIGRAL 132

Query: 114 LANLESGDRTQGASTLTQQLARNLYLSHEKTWTRKAKEALYTMQLEMKYSKDEILNMYLN 173
           L N +SG  ++GASTL+ QL +N+YLS ++ +TRK  EA+  +++E  + KD+IL+MYLN
Sbjct: 133 LVNFKSGGVSEGASTLSMQLVKNIYLSQKRVFTRKLAEAVLAIRVEQVFDKDQILDMYLN 192

Query: 174 QIYYGHGAYGIEAASQMYFGKAAADLDLAESAILAGIPKGPTYYSPYTHMDNAKKRQRII 233
            IY+GH  YGI+ A++ YF K+AADLDLAESA+LAG+ + P  YSP+ +   AK+RQ ++
Sbjct: 193 YIYWGHNNYGIQTAAESYFKKSAADLDLAESAMLAGMIQAPEAYSPFNNFTAAKERQEVV 252

Query: 234 LAAMTEVGDITEAQATAARLETLSFK---PQGEKNTLAAAPYFRDYVRSLATDTLHISSE 290
           L  M  +G IT A+A AA+ E L  K   P   +N+    PY  D V  +A        E
Sbjct: 253 LDRMVSIGWITPAEAEAAKKEPLQNKLGQPTAWQNS--KLPYVTDAV--IAELETRFGKE 308

Query: 291 ELDQGGLNVYTTLDPDMQQAAEDAVEKG-------MDPSGDLETALVSIDPRTGYIKAMV 343
            L QGGL V +T+D D Q+ AE  V+         M  + DL+ ALV+++P T +IKAM 
Sbjct: 309 ALAQGGLRVQSTVDYDTQKLAEKTVKSAYSGLRGRMGRAKDLQIALVAVEPETHFIKAMA 368

Query: 344 GGKNYRTNQFNHALATTRQPGSSFKPIMYLTALSSKSMTGLSVFNSQPTLFHYDNDRKTY 403
           GG +Y  +Q N    + RQPGS+FKP +Y +A +S   T  S    +P      +    Y
Sbjct: 369 GGVDYNKSQLNRVTISRRQPGSAFKPFVYYSAFASGKYTPDSSIEDRPISLR--DGAGFY 426

Query: 404 QPKNFGDKYLGEINMRQAIAASDNIYAVNTIMKVGADKVADMAAKMGIDSPLQTVPSLAL 463
            PKN+G  + G +++RQA+A+S NI AV    +VG +KV + A +MGI +PLQ+V SL L
Sbjct: 427 SPKNYGGGFSGSMSIRQAVASSANIPAVLIGSRVGLNKVIEDAKQMGIKTPLQSVLSLPL 486

Query: 464 GTSPVSPLEMASAFAVIGGGGVKLPVTAILKITDANGKLLYE-APQTQGEKVVEPAAAYV 522
           G+  V+PL+MA  +A +   G     TAIL++T+  G +L +  P+ Q  +V++P AA  
Sbjct: 487 GSVDVTPLDMAVGYATLASNGWYSEPTAILRVTNVRGDVLLDNTPKPQ--QVLDPWAAAS 544

Query: 523 LTRLMEGVFESGGTGNRVASMIKRPVAGKTGTTDTDG--WMVGFTPELSTAVWVGYDKGR 580
            + ++  V + GGTG   ++ + RP AGKTGTTD +   W VG+ P+L+TAVW+G D  R
Sbjct: 545 TSSVLTSVVQ-GGTGK--SAYLGRPTAGKTGTTDNERNVWFVGYVPQLATAVWIGDDANR 601

Query: 581 DI--ATADGRRAAPIFAQFTEKALENVPPKIFPIP 613
            +    + G  AAPI+  F  +A++N+P + FP P
Sbjct: 602 SLGKGISGGGYAAPIWRSFMSQAIKNMPVENFPSP 636


>alr5324 [M] COG0744 Membrane carboxypeptidase (penicillin-binding protein)
          Length = 359

 Score =  381 bits (979), Expect = e-105,   Method: Compositional matrix adjust.
 Identities = 0/581 (0%), Positives = 0/581 (0%), Gaps = 44/581 (7%)

Query: 51  DIRSKLLDS-RGNVLTTFTTDGRSREPVTLNQISPLLIQATLAVEDRKFYKHSGFDVKGM 109
                                  ++   T       L     A  D         D    
Sbjct: 78  LEGFFDARDLPTIS---------AQHFHTAVHNEEQLAALEEASLDEPVTVWMKLDTGMH 128

Query: 110 GRAVLANLESGDRTQGASTLTQQLARNLYLSHEKTWTRKAKEALYTMQLEMKYSKDEILN 169
              V          +       +   N+     +    K       + +   + + +   
Sbjct: 129 RLGVRPEQAEAFYHRLTQCKNVRQPVNIVSHFARADEPKCGATEKQLAIFNTFCEGKPGQ 188

Query: 170 MYLNQIYYGHGAYGIEAASQMYFGKAAADLDLAESAILAGIPKGPTYYSPYTHMDNAKKR 229
             +                            L + +  A     P      + +   + +
Sbjct: 189 RSIAASGGILLWPQSHFDWVRPGIILYGVSPLEDRSTGADFGCQPVMSLTSSLIAVREHK 248

Query: 230 QRIILAAMTEVGDITEAQATAARLETLSFKPQGEKNTL--AAAPYF-----RDYVRSLAT 282
               +          + +     +          +                     ++  
Sbjct: 249 AGEPVGYGGTWVSERDTRLGVVAMGYGDGY---PRAAPSGTPVLVNGREVPIVGRVAMDM 305

Query: 283 DTLHISSEELDQGGLNVYTTLDPDMQQAAEDAVEKG---MDPSG--DLETALVSIDPRTG 337
             +         G        DP +       VE+       S    +      +  +  
Sbjct: 306 ICVD-------LGPQAQDKAGDPVILWGEGLPVERIAEMTKVSAYELITRLTSRVAMKYV 358

Query: 338 YIKAMVGGKNYRTNQFNHALATTRQPGSSFKPIMYLTALSSKSMTGLSVFNSQPTLFHYD 397
                                                                       
Sbjct: 359 --                                                           416

Query: 398 NDRKTYQPKNFGDKYLGEINMRQAIAASDNIYAVNTIMKVGADKVADMAAKMGIDSPLQT 457
                                                                       
Sbjct: 417                                                              476

Query: 458 VPSLALGTSPVSPLEMASAFAVIGGGGVKLPVTAILKITDANGKLLYE-APQTQGEKVVE 516
                                                                       
Sbjct: 477 --                                                           534

Query: 517 PAAAYVLTRLMEGVFESGGTGNRVASMIKRPVAGKTGTTDT--DGWMVGFTPELSTAVWV 574
                                                                       
Sbjct: 535 ---                                                          591

Query: 575 GYDKGRDI--ATADGRRAAPIFAQFTEKALENVPPKIFPIP 613
                                                    
Sbjct: 592                                           632


>sll0002_2 [M] COG0744 Membrane carboxypeptidase (penicillin-binding protein)
          Length = 713

 Score =  365 bits (937), Expect = e-100,   Method: Compositional matrix adjust.
 Identities = 220/546 (40%), Positives = 316/546 (57%), Gaps = 20/546 (3%)

Query: 73  SREPVTLNQISPLLIQATLAVEDRKFYKHSGFDVKGMGRAVLANLESGDRTQGASTLTQQ 132
           S + VT+ +I PL+ QA +A ED +F +H G D++G+ RA L+N++SG   QG ST+TQQ
Sbjct: 74  SHDKVTMGEIPPLVEQAFVASEDSRFREHRGIDLQGIMRASLSNVQSGGVMQGGSTITQQ 133

Query: 133 LARNLYLSHEKTWTRKAKEALYTMQLEMKYSKDEILNMYLNQIYYGHGAYGIEAASQMYF 192
           LAR ++L+ ++T  RK KE     ++E    KD+IL  YLN IY G GAYG+  A+  YF
Sbjct: 134 LARLVFLTQDRTLARKLKEVRLAQKIETALPKDQILERYLNLIYLGSGAYGVADAAHAYF 193

Query: 193 GKAAADLDLAESAILAGIPKGPTYYSPYTHMDNAKKRQRIILAAMTEVGDITEAQATAAR 252
            K   +L L E+A LAG+   P+ YSP  +++ A +R+  +L  M EVG IT A+A AA 
Sbjct: 194 SKTPEELTLGEAATLAGVVPAPSVYSPRQNLELATRRRNEVLNRMAEVGFITPAEAQAAI 253

Query: 253 LETLSFKPQGEKNTLAAAPYFRDYVRSLATDTLHISSEELDQGGLNVYTTLDPDMQQAAE 312
            E L   P   K     A +F DY+  L      +  EEL QGGL V TTL+   Q+ A+
Sbjct: 254 AEPLVINPSPLKRFNREAEFFADYI--LDELRAKLPEEELQQGGLTVNTTLNQQWQEEAQ 311

Query: 313 DAVEKGMDPSGDL----ETALVSIDPRTGYIKAMVGGKNYRTNQFNHALATTRQPGSSFK 368
             +   +   G      E A+V++DPRTG IK MVGGK+Y  +QFN      RQPGS+FK
Sbjct: 312 TVLSDAVKRYGRWQRFSEGAMVAMDPRTGAIKMMVGGKDYDASQFNRVTQAKRQPGSTFK 371

Query: 369 PIMYLTALSSKSMTGLSVFNSQPTLFHYDNDRKTYQPKNFGDKYL-GEINMRQAIAASDN 427
           PI+Y  A++S    G+S   S     +   D   YQP N+GD+Y  G +++  A+ +S N
Sbjct: 372 PILYGAAIAS----GISPNKS---YLNVPIDIGGYQPANYGDRYTGGNMSLTDALTSSVN 424

Query: 428 IYAVNTIMKVGADKVADMAAKMGIDSPLQTVPSLALGTSPVSPLEMASAFAVIGGGGVKL 487
           + AV  ++ VG + V ++A +MGI S L+   SLALG   ++PLEM SA+      G  +
Sbjct: 425 VVAVRLLLDVGWNPVINLARQMGITSKLEPTYSLALGAWEMTPLEMTSAYGTFANKGTHV 484

Query: 488 PVTAILKITDANGKLLYEAPQTQGEKVVEPAAAYVLTRLMEGVFESGGTGNRVASMIKRP 547
              AI  + +A G+++Y+A   Q  + ++P +  +LT +M  V  S GTG R A +  R 
Sbjct: 485 QPYAIQNVVNAQGEVVYKAEHKQ-TQALDPESNAILTSMMRRVVTS-GTG-RPAQLGDRQ 541

Query: 548 VAGKTGTTD--TDGWMVGFTPELSTAVWVGYDKGRDIATADGRRAAPIFAQFTEKALENV 605
           VAGKTGT+D   D W +G+ P+L T VW+G D  R    A    AA ++ QF   A + +
Sbjct: 542 VAGKTGTSDEAKDLWFIGYIPQLVTGVWLGNDNSRPTNGAS-TTAAMVWGQFMRDATKGM 600

Query: 606 PPKIFP 611
           P + FP
Sbjct: 601 PVQSFP 606


  Database: COG
    Posted date:  Aug 3, 2010  9:58 AM
  Number of letters in database: 38,378,431
  Number of sequences in database:  115,334
  
Lambda     K      H
   0.314    0.132    0.381 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 115334
Number of Hits to DB: 49,971,311
Number of extensions: 2117579
Number of successful extensions: 4136
Number of sequences better than 1.0e-05: 111
Number of HSP's gapped: 3760
Number of HSP's successfully gapped: 116
Length of query: 690
Length of database: 38,378,431
Length adjustment: 113
Effective length of query: 577
Effective length of database: 25,345,689
Effective search space: 14624462553
Effective search space used: 14624462553
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 16 ( 7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 42 (22.0 bits)
S2: 119 (50.4 bits)