BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0019.0004.p (168 letters) Database: COG 115,334 sequences; 38,378,431 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value BS_yaaJ [FJ] COG0590 Cytosine/adenosine deaminases 176 1e-44 BS_yfhC [C] COG0778 Nitroreductase 152 2e-37 sll1631 [FJ] COG0590 Cytosine/adenosine deaminases 135 3e-32 PA3767 [FJ] COG0590 Cytosine/adenosine deaminases 128 5e-30 all4872 [FJ] COG0590 Cytosine/adenosine deaminases 126 2e-29 >BS_yaaJ [FJ] COG0590 Cytosine/adenosine deaminases Length = 476 Score = 176 bits (447), Expect = 1e-44, Method: Compositional matrix adjust. Identities = 5/144 (3%), Positives = 18/144 (12%) Query: 19 EYWMXXXXXXXXXXXXLGEVPIGAVVVRDGKIIGRGYNLRETTMDSTAHAEMVAIREASA 78 ++ L ++ +S S Sbjct: 5 FSFINSVLWGSVMIYLLFGAGCWFTFRTGFVQFRYIRQFGKSLKNSIHPQPGGLTSFQSL 64 Query: 79 LLGSWRLLDCHLYVTLEPCPMCAGAMVQSRVPLTVFGTPDPKAGCAGTLMNLLKEPRFNH 138 + + G + + F + Sbjct: 65 CTSLAARVGSGNLAGVALAITAGGPGAVFWMWVAAFIGMATSFAECSLAQLYKERDVNGQ 124 Query: 139 RTEVIQGILQQECAGLLTTFFQQL 162 + + + Sbjct: 125 FRGGPAWYMARGLGMRWMGVLFAV 148 >BS_yfhC [C] COG0778 Nitroreductase Length = 346 Score = 152 bits (384), Expect = 2e-37, Method: Compositional matrix adjust. Identities = 9/156 (5%), Positives = 26/156 (16%), Gaps = 1/156 (0%) Query: 10 LSSEELAVHEYWMXXXXXXXXXXXXLGEVPIGAVVVRDGKIIGRGYNLRETTMDSTAHAE 69 S L + + D + ET + + Sbjct: 12 EVSTLGL-GTMTFGEQNSEADAHAQLDYAVAQGINLIDVAEMYPVPPRPETQGLTETYVG 70 Query: 70 MVAIREASALLGSWRLLDCHLYVTLEPCPMCAGAMVQSRVPLTVFGTPDPKAGCAGTLMN 129 + S + A+ + + + + L Sbjct: 71 NWLAKHGSREKLIIASKVSGPSRNNDKGIRPDQALDRKNIREALHDSLKRLQTDYLDLYQ 130 Query: 130 LLKEPRFNHRTEVIQGILQQECAGLLTTFFQQLRKK 165 + R + + + + Sbjct: 131 VHWPQRPTNCFGKLGYSWTDSAPAVSLLDTLDALAE 166 >sll1631 [FJ] COG0590 Cytosine/adenosine deaminases Length = 164 Score = 135 bits (340), Expect = 3e-32, Method: Compositional matrix adjust. Identities = 64/158 (40%), Positives = 93/158 (58%), Gaps = 1/158 (0%) Query: 12 SEELAVHEYWMXXXXXXXXXXXXLGEVPIGAVVVRD-GKIIGRGYNLRETTMDSTAHAEM 70 + L HE WM +GE+P+GAVVV G+++ G N ++ + AHAEM Sbjct: 2 DQPLPTHEDWMQMAIALAEEAGNVGEIPVGAVVVNAMGEVLATGQNRKQRDQNPIAHAEM 61 Query: 71 VAIREASALLGSWRLLDCHLYVTLEPCPMCAGAMVQSRVPLTVFGTPDPKAGCAGTLMNL 130 +AI+ A LG WRL +C LYVTLEPCPMC GA++Q+R+ L V+GTPDPK G ++ +L Sbjct: 62 LAIQTACRRLGHWRLNECTLYVTLEPCPMCTGAIIQARLGLLVYGTPDPKTGTIDSVFDL 121 Query: 131 LKEPRFNHRTEVIQGILQQECAGLLTTFFQQLRKKSSK 168 NH + + G+ Q++C L +F Q R + + Sbjct: 122 AASAASNHHLQSLGGVCQEQCREQLQDWFAQHRARKKQ 159 >PA3767 [FJ] COG0590 Cytosine/adenosine deaminases Length = 182 Score = 128 bits (321), Expect = 5e-30, Method: Compositional matrix adjust. Identities = 60/131 (45%), Positives = 89/131 (67%) Query: 38 VPIGAVVVRDGKIIGRGYNLRETTMDSTAHAEMVAIREASALLGSWRLLDCHLYVTLEPC 97 VP+GAV+VR+G+IIGRG+N ++ D +AHAEM+AIR A+A G++RL LYVTLEPC Sbjct: 48 VPVGAVLVREGEIIGRGFNRPISSHDPSAHAEMLAIRMAAAEAGNYRLPGSTLYVTLEPC 107 Query: 98 PMCAGAMVQSRVPLTVFGTPDPKAGCAGTLMNLLKEPRFNHRTEVIQGILQQECAGLLTT 157 MC+G +V +R+ V+GT +PK+G + ++ NHR V G+L +EC+ L+ Sbjct: 108 SMCSGLLVHARIQRLVYGTVEPKSGAVESRGRFFEQEHLNHRVMVEGGVLAEECSQALSA 167 Query: 158 FFQQLRKKSSK 168 FF+ R+ + K Sbjct: 168 FFRARREAAKK 178 >all4872 [FJ] COG0590 Cytosine/adenosine deaminases Length = 147 Score = 126 bits (317), Expect = 2e-29, Method: Compositional matrix adjust. Identities = 61/129 (47%), Positives = 82/129 (63%), Gaps = 1/129 (0%) Query: 36 GEVPIGAVVVR-DGKIIGRGYNLRETTMDSTAHAEMVAIREASALLGSWRLLDCHLYVTL 94 GE+P+GAV+ G +I G N +E D TAHAE+VA+R A+ +L +WRL +C LYVTL Sbjct: 15 GEIPVGAVITDASGHLIAEGENRKERDQDPTAHAEIVALRAATKILQTWRLHECTLYVTL 74 Query: 95 EPCPMCAGAMVQSRVPLTVFGTPDPKAGCAGTLMNLLKEPRFNHRTEVIQGILQQECAGL 154 EPCPMCAGA++ SR+ V+G D K G T++N+ NHR +VI GIL+ C Sbjct: 75 EPCPMCAGAIIHSRLGKLVYGVDDTKTGAIRTVLNIPDSAASNHRLQVIGGILESACRQH 134 Query: 155 LTTFFQQLR 163 L +F R Sbjct: 135 LQNWFVNRR 143 Database: COG Posted date: Aug 3, 2010 9:58 AM Number of letters in database: 38,378,431 Number of sequences in database: 115,334 Lambda K H 0.321 0.135 0.405 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 115334 Number of Hits to DB: 10,810,176 Number of extensions: 404792 Number of successful extensions: 654 Number of sequences better than 1.0e-05: 26 Number of HSP's gapped: 645 Number of HSP's successfully gapped: 26 Length of query: 168 Length of database: 38,378,431 Length adjustment: 99 Effective length of query: 69 Effective length of database: 26,960,365 Effective search space: 1860265185 Effective search space used: 1860265185 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.4 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.9 bits) S2: 112 (47.8 bits)