BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0016.0001.p (245 letters) Database: COG 115,334 sequences; 38,378,431 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value BH0092 [EH] COG0115 Branched-chain amino acid aminotransferase/4... 145 6e-35 BS_pabC [EH] COG0115 Branched-chain amino acid aminotransferase/... 131 8e-31 VNG0387G [EH] COG0115 Branched-chain amino acid aminotransferase... 90 3e-18 BH2811 [EH] COG0115 Branched-chain amino acid aminotransferase/4... 81 2e-15 PA2964 [EH] COG0115 Branched-chain amino acid aminotransferase/4... 76 4e-14 >BH0092 [EH] COG0115 Branched-chain amino acid aminotransferase/4-amino-4-deoxychorismate lyase Length = 295 Score = 145 bits (366), Expect = 6e-35, Method: Compositional matrix adjust. Identities = 87/217 (40%), Positives = 122/217 (56%), Gaps = 8/217 (3%) Query: 3 RLAEGCRQLGIPFDGDAVRLRDWIQQVMGKNELQDAYIRYTVTAGKDILGLPAGDYSQPN 62 RL EG R L I + + + + + KN +++AYIR+ V+AG +GL Y +P Sbjct: 50 RLEEGLRALAIDWTYNRSHVLHQLATLCNKNGVRNAYIRWNVSAGPGPIGLYTDTYKEPT 109 Query: 63 HLLYIKELPHLPDSLYREGKGLQLLNLPRNTPEGPVRLKSLHYMNNILAKRXXXXXXXXX 122 ++Y+K LP L G LL L RNTPEG RLKS H++NN+L KR Sbjct: 110 VIIYMKSLPQ---GLSTRKTG-TLLELRRNTPEGDRRLKSHHFLNNVLGKREVAKDPQTE 165 Query: 123 XXXXXXMLTAQGMVAEGIVSNIFFITDNVLYTPDIAAGILPGITREMVLELALAAGIRME 182 LT G++AEG+ SN+F++ + V+YTP + GIL GITR+ VL L GI Sbjct: 166 GI----FLTEDGVLAEGLTSNVFWVKNGVVYTPSVDTGILNGITRQFVLTLCDKEGIPWR 221 Query: 183 AGMYRWEQLAEADEIFLTNSVQEIVPVTTLWRANERQ 219 G Y E L +AD F+TNS+QEIV +T++ +A Q Sbjct: 222 EGRYPKEALLKADGAFITNSIQEIVTLTSVGQAEFNQ 258 >BS_pabC [EH] COG0115 Branched-chain amino acid aminotransferase/4-amino-4-deoxychorismate lyase Length = 269 Score = 131 bits (330), Expect = 8e-31, Method: Compositional matrix adjust. Identities = 0/241 (0%), Positives = 0/241 (0%), Gaps = 14/241 (5%) Query: 1 LDRLAEGCRQLGIPFDGDAVRLRDWIQQVMGKNELQD--AYIRYTVTAGKDILGLPAGDY 58 D + +Q G ++ + Sbjct: 47 QDACQRLMISCDFWPQLEQEMKTLAAEQQNGVLKVVISRGSGGRGYSTLNSGPATRILSV 106 Query: 59 SQPNHLLYIKELPHLPDSLYREGKGLQLLNLPRNTPEGPVRLKSLHYMNNILAKRXXXXX 118 + + +L N + RL+ + +++ Sbjct: 107 TAYPAHYDRLRNEGITLALSPVRL---GRNPHLAGIKHLNRLEQVLIRSHLEQTNADEAL 163 Query: 119 XXXXXXXXXXMLTAQGMVAEGIVSNIFFITDNVLYTPDIAAGILPGITREMVLELALAAG 178 +T T + I L Sbjct: 164 VLDSEG----WVTECCAANLFWRKGNVVYTPRLDQAGVNGIMRQFCIRLLAQSSYQLVEV 219 Query: 179 IRMEAGMYRWEQLAEADEIFLTNSVQEIVPVTTLWRANERQTVSGGCCGRITALLLEAYR 238 + +++ + + V V+ Sbjct: 220 QASLEESLQADEMVICNALMPVMPVCACGDVSFSSATL----- 274 Query: 239 E 239 Sbjct: 275 275 >VNG0387G [EH] COG0115 Branched-chain amino acid aminotransferase/4-amino-4-deoxychorismate lyase Length = 285 Score = 89.7 bits (221), Expect = 3e-18, Method: Compositional matrix adjust. Identities = 71/239 (29%), Positives = 108/239 (45%), Gaps = 16/239 (6%) Query: 3 RLAEGCRQLGIPFDGDAVRLRDWIQQVMGKNELQDAYIRYTVTAGKDILGLPAGDYSQPN 62 RL C LGI LR + + + N+L DAY+R ++T G L D + P Sbjct: 50 RLQATCDSLGIDHGLSEAALRGRVHETLAANDLADAYVRLSITRGVQDGRLTPRDTTDPT 109 Query: 63 HLLYIKELPH---LPDSLYREGKGLQLLNLPRNTPEG-PVRLKSLHYMNNILAKRXXXXX 118 ++ + LP D ++ ++ + ++ P K+ +Y+ +LA+R Sbjct: 110 VVVVVDALPRGGVDGDPVWSMPATVETTTVTQHRDTTIPSTAKTHNYLPGVLARRDIAAD 169 Query: 119 XXXXXXXXXXMLTAQGMVAEGIVSNIFFITDNVLYTPDIAAGILPGITREMVLELALAAG 178 +T G + EG SN+FF+ D LYTP +LPGITR V ELA G Sbjct: 170 ADEAL-----FVTPDGHLTEGAASNLFFVADGTLYTPSGDLDVLPGITRWAVAELAAEHG 224 Query: 179 IRMEAGMYRWEQLAEADEIFLTNSVQEIVPVTTLWRANERQTVSGGCCGRITALLLEAY 237 + + G Y +L +ADE FLTN+ E+ P+ + + C RI A L AY Sbjct: 225 LTVHTGAYTPSRLYDADEAFLTNTTWEVRPIARV------DDATYDTC-RIGAALASAY 276 >BH2811 [EH] COG0115 Branched-chain amino acid aminotransferase/4-amino-4-deoxychorismate lyase Length = 283 Score = 80.9 bits (198), Expect = 2e-15, Method: Compositional matrix adjust. Identities = 71/246 (28%), Positives = 110/246 (44%), Gaps = 30/246 (12%) Query: 1 LDRLAEGCRQLGIPFDGDAVRLRDWIQQVMGKNELQDAYIRYTVTAGKDILGLPAGDYSQ 60 L R E +L +P +L + +QQ++ KN+L+ + + +T G S Sbjct: 49 LTRFKESAEKLDLPMLYSTDKLGELVQQLIEKNKLEHGMVYFQMTRG----------ISP 98 Query: 61 PNHLLYIKELP-------HLPDSLYREGKGLQLLNLPRNTPEGPVRLKSLHYMNNILAKR 113 NHL E P LPD RE L L + R +K+++ + N+LAKR Sbjct: 99 RNHLYTRNETPVLTGFSKPLPDE-KRESVRLYLTDDIRWLR---CDIKTINLLGNVLAKR 154 Query: 114 XXXXXXXXXXXXXXXMLTAQGMVAEGIVSNIFFITDNVLYTPDIAAGILPGITREMVLEL 173 +L G V EG SN+F I + LYT IL GITR++ + L Sbjct: 155 EATDHQCDEA-----LLHRDGTVTEGSSSNVFLIKNETLYTHPATNLILNGITRQITIRL 209 Query: 174 ALAAGIRMEAGMYRWEQLAEADEIFLTNSVQEIVPVTTLWRANERQTVSGGCCGRITALL 233 A A G + + E + +ADE F+T+++ EI PVT + + G +T +L Sbjct: 210 AKAKGYTVVEEPFPKEVIKDADEAFITSTIHEITPVTEVIGDE----TAHFPVGPVTKML 265 Query: 234 LEAYRE 239 +A+ E Sbjct: 266 QQAFAE 271 >PA2964 [EH] COG0115 Branched-chain amino acid aminotransferase/4-amino-4-deoxychorismate lyase Length = 301 Score = 76.3 bits (186), Expect = 4e-14, Method: Compositional matrix adjust. Identities = 20/215 (9%), Positives = 45/215 (20%), Gaps = 14/215 (6%) Query: 1 LDRLAEGCRQLGIPFDGDAVRLRDWIQQVMG-KNELQDAYIRYTVTAGKDILGLPAGDYS 59 R+ + M K + + ++ D+ + Sbjct: 44 RHRIVGIHTEGAYQAIYVDTP-----GLHMEEKRAINRLMNKAASSSIGDVELVIFVVEG 98 Query: 60 QPNHLLYIKELPHLPDSLYREGKGLQLLNLPRNTPEGPV--RLKSLHYMNNILAKRXXXX 117 L L + L + + + + L+ L N L Sbjct: 99 TRWTPDDEMVLNKLREGKAPVI--LAVNKVDNVQEKADLLPHLQFLASQMNFLDI----V 152 Query: 118 XXXXXXXXXXXMLTAQGMVAEGIVSNIFFITDNVLYTPDIAAGILPGITREMVLELALAA 177 + A ++ F + A + L L Sbjct: 153 PISAETGLNVDTIAAIVRKHLPEATHHFPEDYITDRSQRFMASEIIREKLMRFLGAELPY 212 Query: 178 GIRMEAGMYRWEQLAEADEIFLTNSVQEIVPVTTL 212 + +E + + D L +E + Sbjct: 213 SVTVEIERFVSNERGGYDINGLILVEREGQKKMVI 247 Database: COG Posted date: Aug 3, 2010 9:58 AM Number of letters in database: 38,378,431 Number of sequences in database: 115,334 Lambda K H 0.321 0.139 0.416 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 115334 Number of Hits to DB: 17,382,946 Number of extensions: 695837 Number of successful extensions: 1210 Number of sequences better than 1.0e-05: 42 Number of HSP's gapped: 1192 Number of HSP's successfully gapped: 42 Length of query: 245 Length of database: 38,378,431 Length adjustment: 104 Effective length of query: 141 Effective length of database: 26,383,695 Effective search space: 3720100995 Effective search space used: 3720100995 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.4 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.8 bits) S2: 114 (48.5 bits)