BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0013.0003.p (485 letters) Database: COG 115,334 sequences; 38,378,431 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value BS_yabM [R] COG2244 Membrane protein involved in the export of O... 164 4e-40 BH0071 [R] COG2244 Membrane protein involved in the export of O-... 138 2e-32 BH3277 [R] COG2244 Membrane protein involved in the export of O-... 131 3e-30 lin0247 [R] COG2244 Membrane protein involved in the export of O... 127 3e-29 BS_ytgP [R] COG2244 Membrane protein involved in the export of O... 123 6e-28 >BS_yabM [R] COG2244 Membrane protein involved in the export of O-antigen and teichoic acid Length = 432 Score = 164 bits (414), Expect = 4e-40, Method: Compositional matrix adjust. Identities = 24/398 (6%), Positives = 62/398 (15%), Gaps = 32/398 (8%) Query: 33 QGAFILSAAAILSKLIGTLQKIPLQNLGGDAVFGIYNTVYPLYTMLVTVAMLGLPSAVSK 92 + ++A +I + + L + + G +V L +S+ Sbjct: 14 RVTITIAADSIETAVKSELVNVAKKVRIDGFRKGKVPMNIVAQRYGASVRQDVLGDLMSR 73 Query: 93 FVAEASAGRAEQEGRRVLKLSAVLTASSGVLIGILVYAGAPLISVWVGNSHVTPALRSSA 152 +A +E + + + + Sbjct: 74 NFIDAI----IKEKINPAGAPTYVPGEYKLGEDFTYSVEFEVYPEVELQGLEAIEVEKPI 129 Query: 153 WGLAVVPLMAALRGYFQGLHNMVPTAVSQVAEQTVRVAVMIALLLYLTSIHADXXXXXXX 212 + + L + + AE V + ++ Sbjct: 130 VEVTDADVDGMLDTLRKQQATWKEKDGAVEAEDRVTIDFTGSVDGEEFEGGKASDFVLAM 189 Query: 213 XXXXXXXXXXXXXXIMLLYLRQHRRQAEPELAAASETAGGTSWTKEQRQLALENRRERI- 271 E Sbjct: 190 GQGRMIPGFEDGIKGH-----------------------KAGEEFTIDVTFPEEYHAENL 226 Query: 272 ----RIGQLLAYGLPVMLGALAVPLIGLVDVFTVPRLLSSGGSEAAAMAQFGIYNRGLPL 327 + + + M + + Sbjct: 227 KGKAAKFAINLKKVEERELPELTAEFIKRFGVEDGSVEGLRAEVRKNMERELKSAIRNRV 286 Query: 328 VQIVTMLATSLSVVFIPALAEAKYRGDLGLIQTRCSLSLRWFWXXXXXXXXXXXXXXEPV 387 + + +PA L + V Sbjct: 287 KSQAIEGLVKANDIDVPAALIDSEIDVLRRQAAQRFGGNEKQALELPRELFEEQAKRRVV 346 Query: 388 NIALYGDASGSSTMVWLAFTAAGGTVSIISAALLQGLG 425 L G+ ++ + G + SA Sbjct: 347 VGLLLGEVIRTNELKADEERVKGLIEEMASAYEDPKEV 384 >BH0071 [R] COG2244 Membrane protein involved in the export of O-antigen and teichoic acid Length = 432 Score = 138 bits (347), Expect = 2e-32, Method: Compositional matrix adjust. Identities = 22/400 (5%), Positives = 57/400 (14%), Gaps = 22/400 (5%) Query: 31 LLQGAFILSAAAILSKLIGTLQKIPLQNLGGDAVFGIYNTVYPLYTMLVTVAMLGLPSAV 90 L ++ AA + + + + F + ++ Sbjct: 10 GLGRRVTITIAADSIETAVKSELVNVAKKVRIDGFRKGKVPMNIVAQRYGASVRQDVLGD 69 Query: 91 SKFVA-EASAGRAEQEGRRVLKLSAVLTASSGVLIGILVYAGAPLISVWVGNSHVTPALR 149 + + + + G + Sbjct: 70 LMSRNFIDAIIKEKINPAGAPTYVPGEYKLGEDFTYSV-EFEVYPEVELQGLEAIEVEKP 128 Query: 150 SSAWGLAVVPLMAALRGYFQGLHNMVPTAVSQVAEQTVRVAVMIALLLYLTSIHADXXXX 209 A V M Q AV T+ + + +D Sbjct: 129 IVEVTDADVDGMLDTLRKQQATWKEKDGAVEAEDRVTIDFTGSVDGEEFEGGKASDFVLA 188 Query: 210 XXXXXXXXXXXXXXXXXIMLLYLRQHRRQAEPELAAASETAGGTSWTKEQRQLALENRRE 269 + L + + Sbjct: 189 MGQGRMIPGFEDGIKGHKAGE---EFTIDVTFP------------EEYHAENLKGKAAKF 233 Query: 270 RIRIGQLLAYGLPVMLGALAVPLIGLVDVFTVPRLLSSGGSEAA-AMAQFGIYNRGLPLV 328 I + + L L I V + Sbjct: 234 AINLKK----VEERELPELTAEFIKRFGVEDGSVEGLRAEVRKNMERELKSAIRNRVKSQ 289 Query: 329 QIVTMLATSLSVVFIPALAEAKYRGDLGLIQTRCSLSLRWFWXXXXXXXXXXXXXXEPVN 388 I ++ + V + Q + Sbjct: 290 AIEGLVKANDIDVPAALIDSEIDVLRRQAAQRFGGNEKQALELPRELFEEQAKRRVVVGL 349 Query: 389 IALYGDASGSSTMVWLAFTAAGGTVSIISAALLQGLGAVR 428 + + ++ + + Sbjct: 350 LLGEVIRTNELKADEERVKGLIEEMASAYEDPKEVIEFYS 389 >BH3277 [R] COG2244 Membrane protein involved in the export of O-antigen and teichoic acid Length = 432 Score = 131 bits (329), Expect = 3e-30, Method: Compositional matrix adjust. Identities = 22/411 (5%), Positives = 60/411 (14%), Gaps = 26/411 (6%) Query: 29 SKLLQGAFILSAAAILSKLIGTLQKIPLQNLGGDAVFGIYNTVYPLYTMLVTVAMLGLPS 88 S ++ V + +V Sbjct: 4 SVETTQGLGRRVTITIAADSIETAVKSELVNVAKKVRIDGFRKGKVPMNIVAQRYGASVR 63 Query: 89 AVSKFVAEASAGRAEQEGRRVLKLSAVLTASSGVLIGILVYAGAPLISVWV--GNSHVTP 146 + ++ A +G Sbjct: 64 QDVLGDLMSRNFIDAIIKEKINPAGAPTYVPGEYKLGEDFTYSVEFEVYPEVELQGLEAI 123 Query: 147 A-----LRSSAWGLAVVPLMAALRGYFQGLHNMVPTAVSQVAEQTVRVAVMI-ALLLYLT 200 + + + + + + A +V + Sbjct: 124 EVEKPIVEVTDADVDGMLDTLRKQQATWKEKDGAVEAEDRVTIDFTGSVDGEEFEGGKAS 183 Query: 201 SIHADXXXXXXXXXXXXXXXXXXXXXIMLLYLRQHRRQAEPELAAASETAGGTSWTKEQR 260 + + L + + Sbjct: 184 DFVLAMGQGRMIPGFEDGIKGHKAGEEFTIDVTFPEEYHAENLKGKA----------AKF 233 Query: 261 QLALENRRERIRIGQLLAYGLPVMLGALAVPLIGLVDVFTVPRLLSSGGSEAAAMAQFGI 320 + L+ E + L + V + + R ++A + Sbjct: 234 AINLKKVEE-----RELPELTAEFIKRFGVEDGSVEGLRAEVRKNMERELKSAIRNRVKS 288 Query: 321 YNRGLPLVQIVTM-LATSLSVVFIPALAEAKYRGDLGLIQTRCSLSLRWFWXXXXXXXXX 379 + A + +A R Q + Sbjct: 289 QAIEGLVKANDIDVPAALIDSEIDVLRRQAAQRFGGNEKQALELPRELFEEQAKRRVVVG 348 Query: 380 XXXXXEPVNIALYGD--ASGSSTMVWLAFTAAGGTVSIISAALLQGLGAVR 428 L D + V + + + +R Sbjct: 349 LLLGEVIRTNELKADEERVKGLIEEMASAYEDPKEVIEFYSKNKELMDNMR 399 >lin0247 [R] COG2244 Membrane protein involved in the export of O-antigen and teichoic acid Length = 432 Score = 127 bits (320), Expect = 3e-29, Method: Compositional matrix adjust. Identities = 19/406 (4%), Positives = 50/406 (12%), Gaps = 35/406 (8%) Query: 31 LLQGAFILSAAAILSKLIGTLQKIPLQNLGGDAVFGIYNTVYPLYTMLVTVAMLGLPSAV 90 G + A S ++ V A + Sbjct: 9 QGLGRRVTITIAADSIETAVKSELVNVAKKVRIDGFRKGKVPMNIVAQRYGASVRQDVLG 68 Query: 91 SKFVAEASAG-RAEQEGRRVLKLSAVLTASSGVLIGIL-VYAGAPLISVWVGNSHVTPAL 148 E+ + + + V + Sbjct: 69 DLMSRNFIDAIIKEKINPA--GAPTYVPGEYKLGEDFTYSVEF--EVYPEVELQGLEAIE 124 Query: 149 RSSAWGLAVVPLMAALRGYFQGLHNMVPTAVSQVAEQTVRVAVM-----IALLLYLTSIH 203 + + + V + + Sbjct: 125 VEKPIVEVTDADVDGMLDTLRKQQATWKEKDGAVEAEDRVTIDFTGSVDGEEFEGGKASD 184 Query: 204 ADXXXXXXXXXXXXXXXXXXXXXIMLLYLRQHRRQAEPELAAASETAGGTSWTKEQRQLA 263 + E Sbjct: 185 FVLAMGQGRMIPGFED----------GIKGHKAGEEFTIDVTFPEEY------------H 222 Query: 264 LENRRERIRIGQ-LLAYGLPVMLGALAVPLIGLVDVFTVPRLLS-SGGSEAAAMAQFGIY 321 EN + + L L L I V + + Sbjct: 223 AENLKGKAAKFAINLKKVEERELPELTAEFIKRFGVEDGSVEGLRAEVRKNMERELKSAI 282 Query: 322 NRGLPLVQIVTMLATSLSVVFIPALAEAKYRGDLGLIQTRCSLSLRWFWXXXXXXXXXXX 381 + I ++ + V + Q + Sbjct: 283 RNRVKSQAIEGLVKANDIDVPAALIDSEIDVLRRQAAQRFGGNEKQALELPRELFEEQAK 342 Query: 382 XXXEPVNIALYGDASGSSTMVWLAFTAAGGTVSIISAALLQGLGAV 427 + + ++ + + Sbjct: 343 RRVVVGLLLGEVIRTNELKADEERVKGLIEEMASAYEDPKEVIEFY 388 >BS_ytgP [R] COG2244 Membrane protein involved in the export of O-antigen and teichoic acid Length = 432 Score = 123 bits (309), Expect = 6e-28, Method: Compositional matrix adjust. Identities = 25/411 (6%), Positives = 61/411 (14%), Gaps = 29/411 (7%) Query: 29 SKLLQGAFILSAAAILSKLIGTLQKIPLQNLGGDAVFGIYNTVYPLYTMLVTVAMLGLPS 88 + + I + + + I M + G Sbjct: 3 VSVETTQGLGRRVTITIAADSIETAVKSELVNVAKKVRIDGFRKGKVPMNIVAQRYGASV 62 Query: 89 AVSKFVAEASAGRAEQEGRRVLKLSAVLTASSGVLIGILVYAGAPLIS-VWVGN------ 141 S + + + + T G + + Sbjct: 63 RQDVLGDLMSRNFIDAIIKEKINPAGAPTYVPGEYKLGEDFTYSVEFEVYPEVELQGLEA 122 Query: 142 --SHVTPALRSSAWGLAVVPLMAALRGYFQGLHNMVPTAVSQVAEQTVRVAVMIALLLYL 199 + A ++ + + ++ V + T V Sbjct: 123 IEVEKPIVEVTDADVDGMLDTLRKQQATWKEKDGAVEAEDRVTIDFTGSVDGEEFEGGKA 182 Query: 200 TSIHADXXXXXXXXXXXXXXXXXXXXXIMLLYLRQHRRQAEPELAAASETAGGTSWTKEQ 259 + + A + Sbjct: 183 SDFV-LAMGQGRMIPGFEDGIKGHKAGEEFTIDVTFPEEY--HAENLKGKAAKFA----- 234 Query: 260 RQLALENRRERIRIGQLLAYGLPVMLGALAVPLIGLVDVFTVPRLLSSGGSEAAAMAQFG 319 +++ +L + V + + A Sbjct: 235 ------INLKKVEERELPELTAEFIKRFGVEDGSVEGLRAEVRKNMERELKSAIRNRVKS 288 Query: 320 IYNRGL-PLVQIVTMLATSLSVVFIPALAEAKYRGDLGLIQTRCSLSLRWFWXXXXXXXX 378 GL I A S + + A+ G L Sbjct: 289 QAIEGLVKANDIDVPAALIDSEIDVLRRQAAQRFGGNEKQALELPRELFEEQAKRRVVVG 348 Query: 379 XXXXXXEPVNIALYGDAS-----GSSTMVWLAFTAAGGTVSIISAALLQGL 424 N + + S + Sbjct: 349 LLLGEVIRTNELKADEERVKGLIEEMASAYEDPKEVIEFYSKNKELMDNMR 399 Database: COG Posted date: Aug 3, 2010 9:58 AM Number of letters in database: 38,378,431 Number of sequences in database: 115,334 Lambda K H 0.322 0.135 0.390 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 115334 Number of Hits to DB: 28,455,252 Number of extensions: 958920 Number of successful extensions: 3101 Number of sequences better than 1.0e-05: 20 Number of HSP's gapped: 3076 Number of HSP's successfully gapped: 24 Length of query: 485 Length of database: 38,378,431 Length adjustment: 110 Effective length of query: 375 Effective length of database: 25,691,691 Effective search space: 9634384125 Effective search space used: 9634384125 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.4 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.9 bits) S2: 118 (50.1 bits)