BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0012.0031.p (435 letters) Database: COG 115,334 sequences; 38,378,431 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value all1591 [E] COG0141 Histidinol dehydrogenase 341 1e-93 STM1041 [S] COG5281 Phage-related minor tail protein 332 7e-91 SMc02307 [E] COG0141 Histidinol dehydrogenase 318 9e-87 alr3056 [E] COG0141 Histidinol dehydrogenase 301 1e-81 mll6456 [E] COG0141 Histidinol dehydrogenase 299 6e-81 >all1591 [E] COG0141 Histidinol dehydrogenase Length = 433 Score = 341 bits (875), Expect = 1e-93, Method: Compositional matrix adjust. Identities = 187/427 (43%), Positives = 254/427 (59%), Gaps = 6/427 (1%) Query: 4 QSSKEFKLQREVEYGTPEQ----NKAVKEIVADIKQEGDAALLRYTERFDGAALTAAGLR 59 Q+ + +LQR + EQ V+E++ +K++GD A+L YT+ FD L A LR Sbjct: 8 QADVKAELQRICDRTHDEQVLHKEATVREVLQAVKRQGDKAVLHYTDEFDNQILKAEELR 67 Query: 60 VTEEELQAAYGRVEESFVTXXXXXXXXXXXXXXXQKRNSWMDLQPDGTILGQIIRPLKRV 119 VT EL AAY +V + + + SW+ D +LG+ P+ R Sbjct: 68 VTGSELDAAYQQVSQELLEAIQLASRQIEAFHRQRVPKSWVHFGDDDIVLGKRYTPIDRA 127 Query: 120 GVYVPGGKAAYPSSVLMNVIPAQIAGVPEIVMVTPPSTGGTEGIDPYILVAAAEAGVNEI 179 G+YVPGG+AAY S+VLMN IPA++AGVP IV+ TPP G + I+P +LVAA E GV EI Sbjct: 128 GLYVPGGRAAYVSTVLMNAIPARVAGVPRIVIATPP--GAQKAINPAVLVAAQEVGVQEI 185 Query: 180 YRVGGAQAIAALAFGTASIAPVDKICGPGNIYVALAKREVYGAVDIDSIAGPSEIVVXXX 239 YRVGGAQAIAALA+GT +I VD I GPGNIYV LAK+ VYG V IDS+AGPSE+++ Sbjct: 186 YRVGGAQAIAALAYGTETIPKVDVITGPGNIYVTLAKKLVYGNVGIDSLAGPSEVLIIAD 245 Query: 240 XXXXXXXXXXXXXSQAEHDEMASAILVTPSXXXXXXXXXXXXXXXXXLPREAIARASVDN 299 +QAEHD MA+AIL T PR ++ + Sbjct: 246 EGANPVHVATDMLAQAEHDPMAAAILFTTDPALAKNVQVAVERQLVDHPRRIDTEKAIAH 305 Query: 300 YGAIIVVDSLAEGISVVNRLAPEHLEIVTEDPMGLLGSIENAGAVFLGPYSSEPVGDYFA 359 YG I++V+SL + N APEHLE+ +DP +L +I +AGA+FLG + E VGDY A Sbjct: 306 YGLIVLVESLDAAAELSNEFAPEHLELEVKDPWAVLPNIRHAGAIFLGYSTPEAVGDYLA 365 Query: 360 GPNHIIPTNGTARFSSPVDVDDFIKKSSLIYYSKEALLQDGATIIELARREGLEGHARAI 419 GPNH +PT+G AR++S + V+ F+K SS+I YS+ AL + I LA EGL HA ++ Sbjct: 366 GPNHTLPTSGAARYASALSVETFLKHSSIIQYSQTALNKVAGAIDALATAEGLPSHADSV 425 Query: 420 EIRLQNE 426 + R+Q + Sbjct: 426 KRRIQQD 432 >STM1041 [S] COG5281 Phage-related minor tail protein Length = 995 Score = 332 bits (851), Expect = 7e-91, Method: Compositional matrix adjust. Identities = 22/404 (5%), Positives = 59/404 (14%), Gaps = 6/404 (1%) Query: 24 KAVKEIVADIKQEGDAALLRYTERFDGAALTAAGLRVTEEELQAAYGRVEESFVTXXXXX 83 + + + + + ++ A Sbjct: 28 SISAAMAQSQVATRTQSDAMAQLAREANEARERAVDLNQKLRAEA--AQAAVVAQAQDAA 85 Query: 84 XXXXXXXXXXQKRNSWMDLQPDGTILGQIIRPLKRVGVYVPGGKAAYPSSVLMNVIPAQI 143 K+ S + + ++ + Sbjct: 86 AAAFYRQIDSVKQLSGGLQELQRIQAQVRQAKGRGDISQGDYLALVSEAAAKTRELTDAE 145 Query: 144 AGVPEIVMVTPPSTGGTEGIDPYILVAAAEAGVNEIYRVGGAQAIAALAFGTASIAPVDK 203 A + L V + A + + Sbjct: 146 ALATQKKAQFIRRLKE--QTAVQGLSRTELLRVKAAELGVSSAADVYIRKLDTATKSTHA 203 Query: 204 ICGPGNIYVALAKREVYGAVDIDSIAGPSEIVVXXXXXXXXXXXXXXXXSQAEHDEMASA 263 + + + + A + A Sbjct: 204 LGLKSAMARREIGVLIGELARGNFGALRGSGITLANRAGWIEQLMSPKGMMLGGLVGGVA 263 Query: 264 ILVTPSXXXXXXXXXXXXXXXXXLP--REAIARASVDNYGAIIVVDSLAEGISVVNRLAP 321 V L + + + + Sbjct: 264 AAVYGLGKAYYEGAKESEEFNKQLILTGSYAGKTTGKLNEMAKSLAGNGVTQHDAAGVLT 323 Query: 322 EHLEIVTEDPMGLLGSIENAGAVFLGPYSSEPVGDYFAGPNHIIPTNGTARFSSPVDVDD 381 + + + A + S P N + Sbjct: 324 QVVGSGAFTGQAVAMVSRTAARMQENVGQSVDETIRQFKRLQDDPVNAAKELDRALHFLT 383 Query: 382 FIKKSSLIYYSKEALLQDGATIIELARREGLEGHARAIEIRLQN 425 + + ++ D A I A E + A + L Sbjct: 384 ATQLEQIRVLGEQGRTADAAKIAMSAYSEEMNKRAGDVHDNLGW 427 >SMc02307 [E] COG0141 Histidinol dehydrogenase Length = 434 Score = 318 bits (816), Expect = 9e-87, Method: Compositional matrix adjust. Identities = 183/415 (44%), Positives = 244/415 (58%), Gaps = 10/415 (2%) Query: 12 QREVEYGTPEQNKAVKEIVADIKQEGDAALLRYTERFDGAALTAAGLRVTEEELQAAYGR 71 +REV + + N V+ I+ D++ GDAAL Y+ RFDG T G+ VT E+ AA Sbjct: 23 KREV---SEDVNAVVRAIIDDVRARGDAALADYSARFDGIDFTVTGMAVTSAEIDAAIHA 79 Query: 72 VEESFVTXXXXXXXXXXXXXXXQ--KRNSWMDLQPDGTILGQIIRPLKRVGVYVPGGKAA 129 V + Q K + + D G LG P+ VG+YVPGG A+ Sbjct: 80 VAPEVLGALKVAATRIEAHHRRQLPKDDIYEDQM--GVGLGSRWTPIDAVGLYVPGGTAS 137 Query: 130 YPSSVLMNVIPAQIAGVPEIVMVTPPSTGGTEGIDPYILVAAAEAGVNEIYRVGGAQAIA 189 YPSSVLMN +PA++AGVP IVMV P S G I+P +L AA AGV EIYR+GGAQA+A Sbjct: 138 YPSSVLMNALPAKVAGVPRIVMVVPASGGS---INPAVLAAARLAGVEEIYRIGGAQAVA 194 Query: 190 ALAFGTASIAPVDKICGPGNIYVALAKREVYGAVDIDSIAGPSEIVVXXXXXXXXXXXXX 249 ALA+GT +I PV KI GPGN YVA AKR+V+G V ID IAGPSE++V Sbjct: 195 ALAYGTETIEPVAKIVGPGNAYVAAAKRQVFGTVGIDMIAGPSEVLVIADRDNDPDWIAA 254 Query: 250 XXXSQAEHDEMASAILVTPSXXXXXXXXXXXXXXXXXLPREAIARASVDNYGAIIVVDSL 309 +QAEHD A AIL+T LPR A AS ++GA+I+V Sbjct: 255 DLLAQAEHDAGAQAILITDDAAFGDAVEKAVERQLKTLPRAETAAASWRDFGAVILVPDF 314 Query: 310 AEGISVVNRLAPEHLEIVTEDPMGLLGSIENAGAVFLGPYSSEPVGDYFAGPNHIIPTNG 369 + + + NR+APEHLE+ T DP ++ +I NAGA+F+G ++ E +GDY G NH++PT Sbjct: 315 DKAVPLANRIAPEHLELATADPDAMVPAIRNAGAIFIGRHTPEVIGDYVGGSNHVLPTAR 374 Query: 370 TARFSSPVDVDDFIKKSSLIYYSKEALLQDGATIIELARREGLEGHARAIEIRLQ 424 +ARFSS + V D++K++S++ + L G I LAR EGLE HAR++ IRL Sbjct: 375 SARFSSGLGVLDYVKRTSILRLGPDQLRILGPAAIALARSEGLEAHARSVAIRLN 429 >alr3056 [E] COG0141 Histidinol dehydrogenase Length = 359 Score = 301 bits (772), Expect = 1e-81, Method: Compositional matrix adjust. Identities = 0/411 (0%), Positives = 0/411 (0%), Gaps = 29/411 (7%) Query: 26 VKEIVADIKQEGDAALLRYTERFD---------GAALTAAGLRVTEEELQAAYGRVEESF 76 VK A L + F A + E A S Sbjct: 33 VKANAYGHGLLETARTLPDADAFGVARLEEALRLRAGGITKPVLLLEGFFDARDLPTISA 92 Query: 77 VTXXXXXXXXXXXXXXXQKRNSWMDL---QPDGTILGQIIRPLKRVGVYVPGGKAAYPSS 133 + + ++ + +K G + Sbjct: 93 QH--------------FHTAVHNEEQLAALEEASLDEPVTVWMKLDTGMHRLGVRPEQAE 138 Query: 134 VLMNVIPAQIAGVPEIVMVTPPSTGGTEGIDPYILVAAAEAGVNEIYRVGGAQAIAALAF 193 + + + +V+ + A N Q A + Sbjct: 139 AFYHRLTQCKNVRQPVNIVSHFARA---DEPKCGATEKQLAIFNTFCEGKPGQRSIAASG 195 Query: 194 GTASIAPVDKICGPGNIYVALAKREVYGAVDIDSIAGPSEIVVXXXXXXXXXXXXXXXXS 253 G I + + D P + Sbjct: 196 GILLWPQSHFDWVRPGIILYGVSPLEDRSTGADFGCQPVMSLTSSLIAVREHKAGEPVGY 255 Query: 254 QAEHDEMASAILVTPSXXXXXXXXXXXXXXXXXLPREAIARASVDNYGAIIVVDSLAEGI 313 L + L +I VD + Sbjct: 256 GGTWVSERDTRLGVVAMGYGDGYPRAAPSGTPVLVNGREVPIVGRVAMDMICVDLGPQAQ 315 Query: 314 SVVNRLAPEHLEIVTEDPMGLLGSIENAGAVFLGPYSSEPVGDYFAGPNHIIPTNGTARF 373 Sbjct: 316 375 Query: 374 SSPVDVDDFIKKSSLIYYSKEALLQDGATIIELARREGLEGHARAIEIRLQ 424 Sbjct: 376 426 >mll6456 [E] COG0141 Histidinol dehydrogenase Length = 182 Score = 299 bits (766), Expect = 6e-81, Method: Compositional matrix adjust. Identities = 0/427 (0%), Positives = 0/427 (0%), Gaps = 10/427 (2%) Query: 4 QSSKEFKLQREVEYGTPEQ------NKAVKEIVADIKQEGDAALLRYTERFDGAALTAAG 57 + R + + Sbjct: 7 ILLNAQG-VPTGTLEKYAAHTADTRLHLAFSSWLFNAKGQLLVTRRALSKKAWPGVWTNS 65 Query: 58 LRVTEEELQAAYGRVEESFVTXXXXXXXXXXXXXXXQKRNSWMDLQPDGTILGQIIRPLK 117 + + ++ V + + + + Sbjct: 66 VCGHPQLGESNEDAVIRRCRYELGVEITPPESIYPDFRYRATDPSGIVENEVCPVFAART 125 Query: 118 RVGVYVPGGKAAYPSSVLMNVIPAQIAGVPEIVMVTPPSTGGTEGIDPYILVAAAEAGVN 177 + + + + + I P T +A + Sbjct: 126 TSALQINDDEVMDYQWCDLADVLHGIDATPWAFSPWM-VMQAT--NREARKRLSAFTQLK 182 Query: 178 EIYRVGGAQAIAALAFGTASIAPVDKICGPGNIYVALAKREVYGAVDIDSIAGPSEIVVX 237 Sbjct: 183 242 Query: 238 XXXXXXXXXXXXXXXSQAEHDEMASAILVTPSXXXXXXXXXXXXXXXXXLPREAIARASV 297 Sbjct: 243 302 Query: 298 DNYGAIIVVDSLAEGISVVNRLAPEHLEIVTEDPMGLLGSIENAGAVFLGPYSSEPVGDY 357 Sbjct: 303 362 Query: 358 FAGPNHIIPTNGTARFSSPVDVDDFIKKSSLIYYSKEALLQDGATIIELARREGLEGHAR 417 Sbjct: 363 422 Query: 418 AIEIRLQ 424 Sbjct: 423 429 Database: COG Posted date: Aug 3, 2010 9:58 AM Number of letters in database: 38,378,431 Number of sequences in database: 115,334 Lambda K H 0.314 0.134 0.374 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 115334 Number of Hits to DB: 28,999,003 Number of extensions: 1217369 Number of successful extensions: 8051 Number of sequences better than 1.0e-05: 10 Number of HSP's gapped: 8018 Number of HSP's successfully gapped: 10 Length of query: 435 Length of database: 38,378,431 Length adjustment: 109 Effective length of query: 326 Effective length of database: 25,807,025 Effective search space: 8413090150 Effective search space used: 8413090150 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.3 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 42 (21.9 bits) S2: 117 (49.7 bits)