BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0012.0023.p (605 letters) Database: COG 115,334 sequences; 38,378,431 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value lin2622 [R] COG0457 FOG: TPR repeat 125 2e-28 SA0718 [R] COG0457 FOG: TPR repeat 70 1e-11 MA1613 [R] COG0457 FOG: TPR repeat 65 3e-10 NMA1613 [ER] COG1063 Threonine dehydrogenase and related Zn-depe... 65 3e-10 >lin2622 [R] COG0457 FOG: TPR repeat Length = 673 Score = 125 bits (314), Expect = 2e-28, Method: Compositional matrix adjust. Identities = 26/316 (8%), Positives = 48/316 (15%), Gaps = 6/316 (1%) Query: 40 NGNVIPVTLDANFFFERAVRSLDRFQYDKALKNFRKAVEYEPENPVNHCNMAGILSEMGK 99 V VT A + V N A + Sbjct: 8 QQAVEFVTGPCLVLAGAGSGKTRVITNKIAHLIRGCGYQARHIAAVTFTNKAAREMKERV 67 Query: 100 YEASNDILTHVLEEVDPSMTECHFYMANNYANMESFEEAERSLVTYLQEDANGEFIAESE 159 + L A + L + Sbjct: 68 GQTLGRKEARGLMISTFHTLGLDIIKREYAALGMKANFSLFDDTDQLALLKELTEGLIED 127 Query: 160 ELMELLQYEL----NRPAPLVRIRSREGVVEHDRARSLLEEGKFPQAVTLLEEIVQNIPD 215 + + L Q + ++ + G A ++ Sbjct: 128 DKVLLQQLISTISNWKNDLKTPSQAAASAIGERDRIFAHCYGL-YDAHLKACNVLDFDDL 186 Query: 216 FLAAHNNLALAYFYMGRFAKAKECLDEVLAQDPGNLHALCNMAIFLQYAGDREQLAGLVG 275 L L R+ L QD + A Sbjct: 187 ILLPTLLLQANEEVRKRWQNKIRYLLVDEYQDTNTSQYELVKLLVGSRARFTVVGDDDQS 246 Query: 276 MLEATVPFHQEHVFKMATTMGILGKHRTAYGHFRRLLKDEEVGGDASLYHYCAAAASNSG 335 + Q V + + + + + S Sbjct: 247 IYSWRGARPQNLVLLSQDFPALKVIKLEQ-NYRSSGRILKAANILIANNPHVFEKRLFSE 305 Query: 336 FVSEAVRCWQMAAKLD 351 A A + Sbjct: 306 LGYGAELKVLSANNEE 321 >SA0718 [R] COG0457 FOG: TPR repeat Length = 673 Score = 69.7 bits (169), Expect = 1e-11, Method: Compositional matrix adjust. Identities = 9/281 (3%), Positives = 32/281 (11%), Gaps = 36/281 (12%) Query: 39 ENGNVIPVTLDANFFFERAVRSLDRFQYDKALKNFRKAVEYEPENPVNHCNMAGILSEMG 98 G V A + + + + Sbjct: 4 NPGQQQAVEFVTGPCLVLAGAGSGKTRVITNKIAHLIRGCGYQARHIAAVTFTNKAAREM 63 Query: 99 KYEAS------NDILTHVLEEVDPSMTECH---------FYMANNYANMESFEEAERSLV 143 K + + + + E + Sbjct: 64 KERVGQTLGRKEARGLMISTFHTLGLDIIKREYAALGMKANFSLFDDTDQLALLKELTE- 122 Query: 144 TYLQEDANGEFIAESEELMELLQYELNRPAPLVRIRSREGVVEHDRARSLLEEGKFPQAV 203 + ++ + Sbjct: 123 -----GLIEDDKVLLQQ-----LISTISNWKNDLKT----------PSQAAASAIGERDR 162 Query: 204 TLLEEIVQNIPDFLAAHNNLALAYFYMGRFAKAKECLDEVLAQDPGNLHALCNMAIFLQY 263 A + + Q+ + Sbjct: 163 IFAHCYGLYDAHLKACNVLDFDDLILLPTLLLQANEEVRKRWQNKIRYLLVDEYQDTNTS 222 Query: 264 AGDREQLAGLVGMLEATVPFHQEHVFKMATTMGILGKHRTA 304 + +L V + ++ + Sbjct: 223 QYELVKLLVGSRARFTVVGDDDQSIYSWRGARPQNLVLLSQ 263 >MA1613 [R] COG0457 FOG: TPR repeat Length = 673 Score = 65.5 bits (158), Expect = 3e-10, Method: Compositional matrix adjust. Identities = 0/276 (0%), Positives = 0/276 (0%), Gaps = 16/276 (5%) Query: 49 DANFFFERAVRSLDRFQYDKALKNFRKAVEYEPENP--VNHCNMAGILS-EMGKYEASND 105 Sbjct: 1556 1615 Query: 106 ILTHVLEEVDPSMTECHFYMANNYANMESFEEAERSLVTYLQ---EDAN-----GEFIAE 157 Sbjct: 1616 - 1674 Query: 158 SEELMELLQYELNRPAPLVRIRSREGVVEHDRARSLLEEGKFPQAVTLLEEIVQNIPDFL 217 Sbjct: 1675 ---- 1730 Query: 218 AAHNNLALAYFYMGRFAKAKECLDEVLAQDPGNLHALCNMAIFLQYAGDREQLAGLVGML 277 Sbjct: 1731 1790 Query: 278 EATVPFHQEHVFKMATTMGILGKHRTAYGHFRRLLK 313 Sbjct: 1791 1826 Score = 56.6 bits (135), Expect = 1e-07, Method: Compositional matrix adjust. Identities = 15/333 (4%), Positives = 47/333 (14%), Gaps = 41/333 (12%) Query: 19 EAYKGRCLASMIERTSENDVENGNVIPVTLD--ANFFFERAVRSLDRFQYDKALKNFRKA 76 ++ + + T + +S ++ + K Sbjct: 312 LKVLSANNEEHEAERVTGELIAHHFVNKTQYKDYAILYRGNHQSRVFEKFLMQNRIPYKI 371 Query: 77 VEYEPENPVNHCNMAGILSEMGKYEASNDILTHVLEEVDPSMTECHFYMANNYANMESFE 136 + + ++ M + Sbjct: 372 SGGTSFFSRPEIKDLLAYLRVLTNPDDDSAFLRIVN-TPKREIGPATLKKLGEWAMTRNK 430 Query: 137 EAERSLVTYLQEDANG--EFIAESEELMELLQYE--------------------LNRPAP 174 + + A + L + + + Sbjct: 431 SMFTASFDMGLSQTLSGRGYEALTRFTHWLAEIQRLAEREPIAAVRDLIHGMDYESWLYE 490 Query: 175 LVRIRSREGVVE----------------HDRARSLLEEGKFPQAVTLLEEIVQNIPDFLA 218 + + + + + L Sbjct: 491 TSPSPKAAEMRMKNVNQLFSWMTEMLEGSELDEPMTLTQVVTRFTLRDMMERGESEEELD 550 Query: 219 AHNNLALAYFYMGRFAKAKECLDEVLAQDPGNLHALCNMAIFLQYAGDREQLAGLVGMLE 278 + L F E + N+ + A A Sbjct: 551 QVQLMTLHASKGLEFPYVYMVGMEEGFLPHQSSIDEDNIDEERRLAYVGITRAQKELTFT 610 Query: 279 ATVPFHQEHVFKMATTMGILGKHRTAYGHFRRL 311 Q L + + + Sbjct: 611 LCKERRQYGELVRPEPSRFLLELPQDDLIWEQE 643 >NMA1613 [ER] COG1063 Threonine dehydrogenase and related Zn-dependent dehydrogenases Length = 1885 Score = 65.5 bits (158), Expect = 3e-10, Method: Compositional matrix adjust. Identities = 66/276 (23%), Positives = 126/276 (45%), Gaps = 16/276 (5%) Query: 49 DANFFFERAVRSLDRFQYDKALKNFRKAVEYEPENP--VNHCNMAGILS-EMGKYEASND 105 + + + RAV S +++A K+ + + + P P C M GI S E+ YE + Sbjct: 1556 NVDILYSRAVASYKLLDFEEASKDLERLLLFAPGFPDYTKACYMLGIASIELQDYERALQ 1615 Query: 106 ILTHVLEEVDPSMTECHFYMANNYANMESFEEAERSLVTYLQ---EDAN-----GEFIAE 157 L VLE +P+ + + MA N+E +EEA R+ L+ ED G + E Sbjct: 1616 ALDLVLER-EPAHRDALYNMALVLFNLEEYEEAARTFEQLLEASPEDPESLNYLGLCLLE 1674 Query: 158 SEELMELLQYELNRPAPLVRIRSREGVVEHDRARSLLEEGKFPQAVTLLEEIVQNIPDFL 217 E+L E L+ A L ++ E + ++ A +L++ + +++ + I++ P+ Sbjct: 1675 LEDLKEALK--AFEKAALFNPKNEEAL--YNAATTLIKLNRIQESLGYFDRILEISPENY 1730 Query: 218 AAHNNLALAYFYMGRFAKAKECLDEVLAQDPGNLHALCNMAIFLQYAGDREQLAGLVGML 277 A N +A+ + ++ +A + D VL +DP N+ A+ N+ + E A Sbjct: 1731 DAMNYKGVAFCMLEQYREALKSFDNVLKKDPNNIKAVYNVGVVCFKQKLYETAARAFKEA 1790 Query: 278 EATVPFHQEHVFKMATTMGILGKHRTAYGHFRRLLK 313 P+H+ + + ++ G + A F +LL+ Sbjct: 1791 LTINPWHEPSLRYLGLSLAKTGDYEDALKAFEKLLR 1826 Score = 56.6 bits (135), Expect = 1e-07, Method: Compositional matrix adjust. Identities = 77/333 (23%), Positives = 131/333 (39%), Gaps = 41/333 (12%) Query: 19 EAYKGRCLASMIERTSENDVENGNVIPVTLD--ANFFFERAVRSLDRFQYDKALKNFRKA 76 EA+ +C A M E +E + I T ++ RA+ L + +A ++F + Sbjct: 312 EAWNYKCFALMKLEVYEEALEAFDTILETYPDMEEIWYNRALALLKLQNFPEAARSFARV 371 Query: 77 VEYEPENPVNHCNMAGILSEMGKYEASNDILTHVLEEVDPSMTECHFYMANNYANMESFE 136 E +P N +L+ GKYE + + +LE P TE + + FE Sbjct: 372 TELDPGNTDAWFQQGRLLARAGKYEEALETFDRLLE-YHPDFTEAQKFRGTVLTGLGRFE 430 Query: 137 EAERSLVTYLQEDANG--EFIAESEELMELLQYE--------------------LNRPAP 174 EA SL L+++ +I + L+++ ++E +NR Sbjct: 431 EALESLTKSLEKEPENYNMWIQKGLLLLDIGKFEPALEAFENAAGLKPDDEICWMNRGFA 490 Query: 175 LVRIRSREGVVE----------------HDRARSLLEEGKFPQAVTLLEEIVQNIPDFLA 218 L + E +E + + L + GK +A+ EE V+ PDF Sbjct: 491 LYSLECYEEALEAFKEGLHLNPYLEKGWNKKGIVLGKLGKTEEALEAFEEAVKLRPDFED 550 Query: 219 AHNNLALAYFYMGRFAKAKECLDEVLAQDPGNLHALCNMAIFLQYAGDREQLAGLVGMLE 278 A N+ L F + KA+E EVL +P +L AL N I L G E + + Sbjct: 551 AWKNMGLLLFASEEYEKAEEAFAEVLKTNPEDLDALYNRGISLLRLGRNETALEYLEKVV 610 Query: 279 ATVPFHQEHVFKMATTMGILGKHRTAYGHFRRL 311 + P + + + + + LG+ A F +L Sbjct: 611 SLSPDYPDLAYSLGVALMELGELEKALETFEKL 643 Database: COG Posted date: Aug 3, 2010 9:58 AM Number of letters in database: 38,378,431 Number of sequences in database: 115,334 Lambda K H 0.319 0.134 0.401 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 115334 Number of Hits to DB: 44,870,570 Number of extensions: 1994342 Number of successful extensions: 13778 Number of sequences better than 1.0e-05: 12 Number of HSP's gapped: 13709 Number of HSP's successfully gapped: 24 Length of query: 605 Length of database: 38,378,431 Length adjustment: 112 Effective length of query: 493 Effective length of database: 25,461,023 Effective search space: 12552284339 Effective search space used: 12552284339 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.4 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.8 bits) S2: 119 (50.4 bits)