BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0012.0021.p (564 letters) Database: COG 115,334 sequences; 38,378,431 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value BS_thiC [H] COG0352 Thiamine monophosphate synthase 786 0.0 STM0788 [E] COG0010 Arginase/agmatinase/formimionoglutamate hydr... 357 2e-98 MTH1576 [H] COG0422 Thiamine biosynthesis protein ThiC 346 5e-95 MA1790 [H] COG0422 Thiamine biosynthesis protein ThiC 330 3e-90 MTH1543 [H] COG0422 Thiamine biosynthesis protein ThiC 303 4e-82 >BS_thiC [H] COG0352 Thiamine monophosphate synthase Length = 631 Score = 786 bits (2031), Expect = 0.0, Method: Compositional matrix adjust. Identities = 387/578 (66%), Positives = 439/578 (75%), Gaps = 37/578 (6%) Query: 13 SGFPASRKVYVEGSRPDIQVPMREICLSRTEGVAGDSDNAP-------LRVYDTSGIYTD 65 + FP S+++Y+ G+ P ++VPMREI LS T + G S P + VYDTSG Y D Sbjct: 25 TAFPNSKRIYITGTHPGVRVPMREIQLSPT--LIGGSKEQPQYEENEAIPVYDTSGPYGD 82 Query: 66 DGQETDIRKGLPPHRLCWIAERGDSEEYAGRAVQPGDNGRTVDGLPEEGYSGLKRRPLRA 125 ++++GL R WI RGD+EE R+ DGL E +SG+ P RA Sbjct: 83 PQIAINVQQGLAKLRQPWIDARGDTEELTVRSSDYTKARLADDGLDELRFSGV-LTPKRA 141 Query: 126 REGRNVTQLHYARQGIITPEMEYIAIREN---------------------------TRPE 158 + GR VTQLHYARQGIITPEME+IAIREN E Sbjct: 142 KAGRRVTQLHYARQGIITPEMEFIAIRENMGRERIRSEVLRHQHPGMSFGAHLPENITAE 201 Query: 159 FVRDEVAAGRAIIPSNLNHPESEPMIIGRNFLVKINANIGNSAVSSSIDEEVEKMRWATR 218 FVRDEVAAGRAIIP+N+NHPESEPMIIGRNFLVK+NANIGNSAV+SSI+EEVEK+ W+TR Sbjct: 202 FVRDEVAAGRAIIPANINHPESEPMIIGRNFLVKVNANIGNSAVTSSIEEEVEKLVWSTR 261 Query: 219 WGADTIMDLSTGAKIHATREWIIRNSPVPIGTVPIYQALEKVKGIAEDLTWELYRDTLIE 278 WGADT+MDLSTG IH TREWI+RNSPVPIGTVPIYQALEKV GIAEDLTWE +RDTL+E Sbjct: 262 WGADTVMDLSTGRYIHETREWILRNSPVPIGTVPIYQALEKVNGIAEDLTWEAFRDTLLE 321 Query: 279 QAEQGVDYFTIHAGVLKRYIPLTAGRMTGIVSRGGSIMAAWCLAHDQENFLYTHFEEICE 338 QAEQGVDYFTIHAGVL RY+P+TA R+TGIVSRGGSIMA WCL+H QENFLY HF EICE Sbjct: 322 QAEQGVDYFTIHAGVLLRYVPMTAKRLTGIVSRGGSIMAKWCLSHHQENFLYQHFREICE 381 Query: 339 IMKTYDVAFSLGDGLRPGSIADANDEAQFXXXXXXXXXXXXXWKHDVQVMVEGPGHVPMH 398 I YDV+ SLGDGLRPGSI DANDEAQF W++DVQVM+EGPGHVPM Sbjct: 382 ICAAYDVSLSLGDGLRPGSIQDANDEAQFAELHTLGELTKIAWEYDVQVMIEGPGHVPMQ 441 Query: 399 RIKENMDKQLELCREAPFYTLGPLTTDIAPGYDHITSAIGAAMIGWFGTAMLCYVTPKEH 458 I+ NM ++LE C EAPFYTLGPLTTDIAPGYDH TS IGAAMIGWFG AMLCYVTPKEH Sbjct: 442 MIRRNMTEELEHCHEAPFYTLGPLTTDIAPGYDHFTSGIGAAMIGWFGCAMLCYVTPKEH 501 Query: 459 LGLPNKNDVREGVITYKIAAHAADLAKGHPGAQDRDNALSKARFEFRWRDQFHLSLDPER 518 LGLPNK DV++G+ITYKIAAHAADLAKGHPGAQ RDNA+SKARFEFRW DQF+L+LDP Sbjct: 502 LGLPNKEDVKQGLITYKIAAHAADLAKGHPGAQIRDNAMSKARFEFRWEDQFNLALDPFT 561 Query: 519 ALEYHDETLPAEGAKTAHFCSMCGPKFCSMRISHDIRN 556 A YHDETLP E K AHFCSMCGPKFCSM+IS ++R+ Sbjct: 562 ARAYHDETLPQESGKVAHFCSMCGPKFCSMKISQEVRD 599 >STM0788 [E] COG0010 Arginase/agmatinase/formimionoglutamate hydrolase, arginase family Length = 252 Score = 357 bits (917), Expect = 2e-98, Method: Compositional matrix adjust. Identities = 0/430 (0%), Positives = 0/430 (0%), Gaps = 13/430 (3%) Query: 131 VTQLHYARQGIITPEMEYIAIRENTRPEFVRDEVAAGRAIIPSNLNHPESEPMIIGRNFL 190 ++ + + + E A+ R E A P N + R Sbjct: 1 MSDWNPSLYLHFSAERSRPAVELLARVPLENVEYVADLGCGPGNSTALLQQRWPAARITG 60 Query: 191 VKINANIGNSAVSSSIDEEVEKMRWATRWGADTIMDLSTGAKIHATREWIIRNSPVPIGT 250 + + + A S+ D + + + + A + + + Sbjct: 61 IDSSPAMIAEARSALPDCQFVEADIRNWQPVQALDLIFANASLQWLPDHYELFPHLVSLL 120 Query: 251 VPIYQALEK---VKGIAEDLTWELYRDTLIEQAEQGVDYFTIHAGVLKRYIP--LTAGRM 305 P + + +HA Sbjct: 121 NPQGVLAVQMPDNWLEPTHVLMREVAWEQNYPDRGREPLAGVHAYYDILSEAGCEVDIWR 180 Query: 306 TGIVSRGGSIMAAWCLAHDQENFLYTHFEEICEIMKTYDVAFSLGDGLRPGSIADANDEA 365 T + S A + E + + P A Sbjct: 181 TTYYHQMPSHQAIIDWVTATGLRPWLQDLTESEQQLFLKRYHQMLEEQYPLQENGQILLA 240 Query: 366 QFXXXXXXXXXXXXXWKHDVQVMVEGPGHVPMHRIKENMDKQLELCREAPFYTLGPLTTD 425 Sbjct: 241 300 Query: 426 IAPGYDHITSAIGAAMIGWFGTAMLCYVTPKEHLGLPNKNDVREGVITYKIAAHAADLAK 485 Sbjct: 301 360 Query: 486 GHPGAQDRDNALSKARFEFRWRDQFHLSLDPERA-LEYHDETLPAEGAKTAHFCSMCGPK 544 Sbjct: 361 ------- 413 Query: 545 FCSMRISHDI 554 Sbjct: 414 423 >MTH1576 [H] COG0422 Thiamine biosynthesis protein ThiC Length = 268 Score = 346 bits (888), Expect = 5e-95, Method: Compositional matrix adjust. Identities = 0/430 (0%), Positives = 0/430 (0%), Gaps = 11/430 (2%) Query: 131 VTQLHYARQGIITPEMEYIAIRENTRPEFVRDEVAAGRAIIPSNLNHPESEPMIIGRNFL 190 + ++ + + E ++ T + A+ + + H + Sbjct: 1 MNRVDLSIFIPDSLTAETGDLKIKTYKVVLIARAASIFGVKRIVIYH-DDADGEARFIRD 59 Query: 191 VKINANIGNSAVSSSIDEEVEKMRWATRWGADTIMDLSTGAKIHATREWIIRNSPVPIGT 250 + + E T + R+ + Sbjct: 60 ILTYMDTPQYLRRKVFPIMRELKHVGILPPLRTPHHPTGKPVTGEYRQGLTVKRVKKGTL 119 Query: 251 VPIYQALEKV---KGIAEDLTWELYRDTLIEQAEQGVDYFTIHAGVLKRYIPLT--AGRM 305 V I + K + E + + + G Sbjct: 120 VDIGADKLALCREKLTVNRIMSFRVVRLGKEILIEPDEPEDRYWGYEVLDTRRNLAESLK 179 Query: 306 TGIVSRGGSIMAAWCLAHDQENFLYTHFEEICEIMKTYDVAFSLGDGLRPGSIADANDEA 365 T + + + T E + + + + Sbjct: 180 TVGADVVVATSRNASPITSILDEVKTRMRGAREAAILFGGPYKGLPEIDADIWVNTLPGQ 239 Query: 366 QFXXXXXXXXXXXXXWKHDVQVMVEGPGHVPMHRIKENMDKQLELCREAPFYTLGPLTTD 425 Sbjct: 240 299 Query: 426 IAPGYDHITSAIGAAMIGWFGTAMLCYVTPKEHLGLPNKNDVREGVITYKIAAHAADLAK 485 Sbjct: 300 359 Query: 486 GHPGAQDRDNALSKARFEFRWRDQFHLSLDPERALEYHDETLPAEGAKTAHFCSMCGPKF 545 Sbjct: 360 ----- 414 Query: 546 CSMRISHDIR 555 Sbjct: 415 424 >MA1790 [H] COG0422 Thiamine biosynthesis protein ThiC Length = 428 Score = 330 bits (847), Expect = 3e-90, Method: Compositional matrix adjust. Identities = 183/426 (42%), Positives = 252/426 (59%), Gaps = 16/426 (3%) Query: 131 VTQLHYARQGIITPEMEYIAIRENTRPEFVRDEVAAGRAIIPSNLNHPESEPMIIGRNFL 190 +T + A++GI+TP +E +A E E VR VA G IP N N E+ P+ IG+ Sbjct: 1 MTLMEDAKKGIVTPSIEAVAKAEGIDAETVRSCVAKGLIAIPKN-NRRETLPIGIGKYMS 59 Query: 191 VKINANIGNSAVSSSIDEEVEKMRWATRWGADTIMDLSTGAKIHATREWIIRNSPVPIGT 250 KINAN+G S ID EVEK + A +GA +MDLSTG ++ R I++ +P+GT Sbjct: 60 TKINANVGTSRDCIDIDAEVEKAKAAEAFGAHAVMDLSTGGDLNEIRTRILKAVNIPVGT 119 Query: 251 VPIYQALEKVKGIAEDLTWELYRDTLIEQAEQGVDYFTIHAGV----LKRYIPLTAGRMT 306 VPIYQA K + E + +++ + + + AEQGVD+ T+HAGV L+R + R+ Sbjct: 120 VPIYQAAASQKVVVEMTSDDMF-NAVRKHAEQGVDFVTVHAGVNLNSLERL--RQSDRIM 176 Query: 307 GIVSRGGSIMAAWCLAHDQENFLYTHFEEICEIMKTYDVAFSLGDGLRPGSIADANDEAQ 366 +VSRGGS AW L + ++N Y F+ + EI K YD+ SLGDG+RPG IADA+D + Sbjct: 177 NVVSRGGSFTLAWMLHNGEDNPFYAEFDYLLEIAKEYDMTLSLGDGMRPGCIADASDRPK 236 Query: 367 FXXXXXXXXXXXXXWKHDVQVMVEGPGHVPMHRIKENMDKQLELCREAPFYTLGPLTTDI 426 F +VQ VEGPGHVP++ I+ ++ ELC AP Y LGPL TDI Sbjct: 237 FMEFITLGELVKRSRAANVQTFVEGPGHVPLNEIELSVRGMKELCDGAPLYLLGPLVTDI 296 Query: 427 APGYDHITSAIGAAMIGWFGTAMLCYVTPKEHLGLPNKNDVREGVITYKIAAHAADLAKG 486 APG+DHIT AIG A+ G +GT LC VTP EHL LP+ D++EG+ K+AAH DL K Sbjct: 297 APGFDHITGAIGGAVAGMYGTDFLCMVTPSEHLALPSLEDIKEGLFVTKLAAHTIDLIKE 356 Query: 487 HP--GAQDRDNALSKARFEFRWRDQFHLSLDPERALEYHDETLPAEGAKTAHFCSMCGPK 544 P A +D A++ AR + W QF L++D +RA + D A CSMCG + Sbjct: 357 GPRERAWRQDTAMAYARRDLDWEKQFELAIDGDRARKIRDARKTESDA-----CSMCG-E 410 Query: 545 FCSMRI 550 C+++I Sbjct: 411 LCAVKI 416 >MTH1543 [H] COG0422 Thiamine biosynthesis protein ThiC Length = 268 Score = 303 bits (776), Expect = 4e-82, Method: Compositional matrix adjust. Identities = 0/439 (0%), Positives = 0/439 (0%), Gaps = 23/439 (5%) Query: 131 VTQLHYARQGIITPEMEYIAIRENTRPEFVRDEVAAGRAIIPSNLNHPESEPMIIGRNFL 190 + ++ + + E ++ T + A+ + + H +++ + Sbjct: 1 MNRVDLSIFIPDSLTAETGDLKIKTYKVVLIARAASIFGVKRIVIYHDDADGEARFIRDI 60 Query: 191 VKINANIGNSAVSSSIDEEVEKMRWATRWGADTIMDLSTGAKIHATREWIIRNSPVPIGT 250 + K + ++ Sbjct: 61 LTYMDTPQYLRRKVFPIMRELKHVGILPPLRTPHHPTGKPVTGEYRQGLTVKRVKKGTLV 120 Query: 251 VPIYQA-LEKV--KGIAEDLTWELYRDTLIEQAEQGVDYFTIHAGV-------LKRYIPL 300 + +++ + R E L Sbjct: 121 DIGADKLALCREKLTVNRIMSFRVVRLGKEILIEPDEPEDRYWGYEVLDTRRNLAES--- 177 Query: 301 TAGRMTGIVSRGGSIMAAWCLAHDQENFLYTHFEEICEIMKTYDVAFSLGDGLRPGSIAD 360 + G + A + + + L + + Sbjct: 178 --LKTVGADVVVATSRNASPITSILDEVKTRMRGAREAAILFGGPYKGLPEIDADIWVNT 235 Query: 361 ANDEAQFXXXXXXXXXXXXXWKHDVQVMVEGPGHVPMHRIKENMDKQLELCREAPFYTLG 420 Sbjct: 236 295 Query: 421 PLTTDIAPGYDHITSAIGAAMIGWFGTAMLCYVTPKEHLGLPNKNDVREGVITYKIAAHA 480 Sbjct: 296 355 Query: 481 ADLAKG-HPGAQDRDNALSKARFEFRWRDQFHLSLDPERALEYHDETLPAEGAKTAHFCS 539 Sbjct: 356 ------ 409 Query: 540 MCGPKFCSMRISHDIRNSS 558 Sbjct: 410 - 427 Database: COG Posted date: Aug 3, 2010 9:58 AM Number of letters in database: 38,378,431 Number of sequences in database: 115,334 Lambda K H 0.319 0.136 0.414 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 115334 Number of Hits to DB: 43,734,454 Number of extensions: 1838968 Number of successful extensions: 2642 Number of sequences better than 1.0e-05: 9 Number of HSP's gapped: 2609 Number of HSP's successfully gapped: 9 Length of query: 564 Length of database: 38,378,431 Length adjustment: 112 Effective length of query: 452 Effective length of database: 25,461,023 Effective search space: 11508382396 Effective search space used: 11508382396 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.4 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.7 bits) S2: 118 (50.1 bits)