BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0012.0018.p (316 letters) Database: COG 115,334 sequences; 38,378,431 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value BH1425 [KG] COG1940 Transcriptional regulator/sugar kinase 219 4e-57 L84096 [KG] COG1940 Transcriptional regulator/sugar kinase 196 4e-50 BS_glcK [KG] COG1940 Transcriptional regulator/sugar kinase 195 1e-49 SP0668 [KG] COG1940 Transcriptional regulator/sugar kinase 193 3e-49 lin1376 [KG] COG1940 Transcriptional regulator/sugar kinase 187 2e-47 >BH1425 [KG] COG1940 Transcriptional regulator/sugar kinase Length = 330 Score = 219 bits (558), Expect = 4e-57, Method: Compositional matrix adjust. Identities = 133/320 (41%), Positives = 183/320 (57%), Gaps = 5/320 (1%) Query: 1 MSESIYVGVDLGGTTIKVGICNAEGNLLHTYEGPTGTADGVDAVIDNIEKYVRQIVEDSP 60 MS+ YVGVD+GGTTIK+ G ++ +E PT DG + NI + + + Sbjct: 1 MSDRWYVGVDVGGTTIKMAFLTTAGEIVDKWEIPTNKQDGGALITTNIADALDKRLSGHH 60 Query: 61 YSWDQLAGVGAGLAGFTNIREGIIILAPNIGFRDVPIRSILEGRLNKPVKIDNDANVAAL 120 S L G+G G GF + G I A NIG+RD P++ LE PV +DNDAN+AAL Sbjct: 61 KSKSDLIGIGLGAPGFIEMDTGFIYHAVNIGWRDFPLKDKLEEETKLPVIVDNDANIAAL 120 Query: 121 GEAWSGAGRGVENCVCYTLXXXXXXXXXXXXKVYQGFAGLAGELGHISVVPDLEAIQCGC 180 GE W GAG G +N + TL + G G+AGE+GHI+V+P+ A C C Sbjct: 121 GEMWKGAGDGAKNMLLITLGTGVGGGIVANGNILHGVNGMAGEIGHITVIPEGGA-PCNC 179 Query: 181 GNMGCLETVSSATGIIRMANDAVSRGDRTSLSM-VEK---IAAKEVFDAAKAGDEVALRI 236 G GCLETV+SATGI R+A + V+ + L++ +K + AK+VF AA A D AL + Sbjct: 180 GKTGCLETVASATGIARIATEGVTEHKESQLALDYDKHGVLTAKDVFSAADASDAFALSV 239 Query: 237 VNRAAFYLGKSMAAVAAVLNPEVFIVGGGVSKAGDILFDEVRRVFAKLAPAPLQTGVTIV 296 V+ A+YLG ++A +A LNPE ++GGGVSKAGD L +++ F A + G Sbjct: 240 VDHIAYYLGFAIANLANALNPEKIVIGGGVSKAGDTLLKPIKQHFEAYALPRVADGAEFR 299 Query: 297 PAELGNDAGIIGAAGLLLRS 316 A LGNDAG+IG L+ + Sbjct: 300 IATLGNDAGVIGGGWLVKQQ 319 >L84096 [KG] COG1940 Transcriptional regulator/sugar kinase Length = 323 Score = 196 bits (499), Expect = 4e-50, Method: Compositional matrix adjust. Identities = 129/325 (39%), Positives = 182/325 (56%), Gaps = 16/325 (4%) Query: 1 MSESIYVGVDLGGTTIKVGICNAEGNLLHTYEGPTGT-ADGVDAVIDNIEKYVRQIVEDS 59 M+ S +G+DLGGT+IK GI +G + + PT DG V D IE ++ S Sbjct: 1 MTASKIIGIDLGGTSIKFGILTLDGEVQDKWSIPTNILEDGKHIVPDIIESINHRL---S 57 Query: 60 PYSWD--QLAGVGAGLAGFTNIREGIIILAPNIGFRDVPI--RSILEGRLNKPVKIDNDA 115 Y+ D + G+G G G NI E + A N+ + D + I EG + P +DNDA Sbjct: 58 LYNLDKSEFLGIGMGTPGSVNISESTVKAAFNLNWADTQEVGKPISEG-VGLPFILDNDA 116 Query: 116 NVAALGEAWSGAGRGVENCVCYTLXXXXXXXXXXXXKVYQGFAGLAGELGHISVVPDLEA 175 NVAALGE W GAG + V TL ++ G AG GE+GHI V P Sbjct: 117 NVAALGERWVGAGENNPDVVFITLGTGVGGGIIASGELVHGVAGAGGEIGHICVDP--YG 174 Query: 176 IQCGCGNMGCLETVSSATGIIRMANDAVSR--GDRTSLSMVE---KIAAKEVFDAAKAGD 230 +C CGN GCLETV+SATGI+R+A GD T + ++ ++ +K++F AA+ GD Sbjct: 175 FECTCGNRGCLETVTSATGIVRLARKFAEEYEGDSTIKAAIDNGDEVTSKDIFFAAQEGD 234 Query: 231 EVALRIVNRAAFYLGKSMAAVAAVLNPEVFIVGGGVSKAGDILFDEVRRVFAKLAPAPLQ 290 +L +V++ A+YLG + A + + LNP ++GGGVS AG+ L ++V + F K A + ++ Sbjct: 235 HFSLSVVDKFAYYLGFACANLGSTLNPASIVIGGGVSAAGEFLREKVEKYFTKYAFSTVR 294 Query: 291 TGVTIVPAELGNDAGIIGAAGLLLR 315 I A LGNDAGIIGAA L L+ Sbjct: 295 NSSKIKLAVLGNDAGIIGAASLALK 319 >BS_glcK [KG] COG1940 Transcriptional regulator/sugar kinase Length = 321 Score = 195 bits (495), Expect = 1e-49, Method: Compositional matrix adjust. Identities = 112/311 (36%), Positives = 175/311 (56%), Gaps = 4/311 (1%) Query: 1 MSESIYVGVDLGGTTIKVGICNAEGNLLHTYEGPTGTADGVDAVIDNIEKYVRQIVEDSP 60 M E + G+DLGGTTIK+ N G + H +E PT D + I K + +++ Sbjct: 1 MDEIWFAGIDLGGTTIKLAFINQYGEIQHKWEVPTDKTG--DTITVTIAKTIDSKLDELQ 58 Query: 61 YSWDQLAGVGAGLAGFTNIREGIIILAPNIGFRDVPIRSILEGRLNKPVKIDNDANVAAL 120 + +G G G ++ G++ N+G+++ +++ LE P I+NDAN+AAL Sbjct: 59 KPKHIIKYIGMGAPGPVDMAAGVVYETVNLGWKNYALKNHLETETGIPAVIENDANIAAL 118 Query: 121 GEAWSGAGRGVENCVCYTLXXXXXXXXXXXXKVYQGFAGLAGELGHISVVPDLEAIQCGC 180 GE W GAG G ++ + TL ++ G G GE+GHI +P+ A C C Sbjct: 119 GEMWKGAGDGAKDVILVTLGTGVGGGIIANGEILHGINGAGGEIGHICSIPEGGA-PCNC 177 Query: 181 GNMGCLETVSSATGIIRMANDAVSRGDRTS-LSMVEKIAAKEVFDAAKAGDEVALRIVNR 239 G GC+ET++SATGI+R+A ++ +T+ L E+++A++VF+AA DE+AL +V+ Sbjct: 178 GKTGCIETIASATGIVRIAKGKIANAKKTTRLKATEQLSARDVFEAAGENDEIALEVVDY 237 Query: 240 AAFYLGKSMAAVAAVLNPEVFIVGGGVSKAGDILFDEVRRVFAKLAPAPLQTGVTIVPAE 299 A +LG + +A+ LNP ++GGGVS+AG++L +V + F K A I A Sbjct: 238 VAKHLGLVLGNLASSLNPSKIVLGGGVSRAGELLRSKVEKTFRKCAFPRAAQAADISIAA 297 Query: 300 LGNDAGIIGAA 310 LGNDAG+IG A Sbjct: 298 LGNDAGVIGGA 308 >SP0668 [KG] COG1940 Transcriptional regulator/sugar kinase Length = 320 Score = 193 bits (491), Expect = 3e-49, Method: Compositional matrix adjust. Identities = 121/321 (37%), Positives = 180/321 (56%), Gaps = 8/321 (2%) Query: 1 MSESIYVGVDLGGTTIKVGICNAEGNLLHTYEGPTGTADGVDAVIDNIEKYVRQIVEDSP 60 MS+ I +G+DLGGT+IK I G + + T D ++D++ + ++ ++ Sbjct: 2 MSQKI-IGIDLGGTSIKFAILTTAGEIQGKWSIKTNILDEGSHIVDDMIESIQHRLDLLG 60 Query: 61 YSWDQLAGVGAGLAGFTNIREGIIILAPNIGFRDV-PIRSILEGRLNKPVKIDNDANVAA 119 + G+G G G + +G +I A N+ ++ + PI+ +E L P IDNDANVAA Sbjct: 61 LAAADFQGIGMGSPGVVDRDKGTVIGAYNLNWKTLQPIKQKIEKALGIPFFIDNDANVAA 120 Query: 120 LGEAWSGAGRGVENCVCYTLXXXXXXXXXXXXKVYQGFAGLAGELGHISVVPDLEAIQCG 179 LGE W GAG + V TL K+ G AG AGELGHI+V D + I C Sbjct: 121 LGERWMGAGDNQPDVVFITLGTGVGGGIVAEGKLLHGVAGAAGELGHITVDFD-QPISCT 179 Query: 180 CGNMGCLETVSSATGIIRMANDAVSR--GDRTSLSMV---EKIAAKEVFDAAKAGDEVAL 234 CG GCLETV+SATGI+ + GD ++ E++ AK VFD AK GD++AL Sbjct: 180 CGKKGCLETVASATGIVNLTRRYADEYEGDAALKRLIDNGEEVTAKTVFDLAKEGDDLAL 239 Query: 235 RIVNRAAFYLGKSMAAVAAVLNPEVFIVGGGVSKAGDILFDEVRRVFAKLAPAPLQTGVT 294 + + YLG + A + ++LNP ++GGGVS AG+ L V++V+ + + ++T Sbjct: 240 IVYRNFSRYLGIACANIGSILNPSTIVIGGGVSAAGEFLLQGVQKVYDENSFPQVRTSTK 299 Query: 295 IVPAELGNDAGIIGAAGLLLR 315 + A LGNDAG+IGAA L+L+ Sbjct: 300 LALATLGNDAGVIGAASLVLQ 320 >lin1376 [KG] COG1940 Transcriptional regulator/sugar kinase Length = 322 Score = 187 bits (475), Expect = 2e-47, Method: Compositional matrix adjust. Identities = 117/314 (37%), Positives = 168/314 (53%), Gaps = 7/314 (2%) Query: 7 VGVDLGGTTIKVGICNAEGNLLHTYEGPTGTADGVDAVIDNIEKYVRQIVEDSPYSWDQL 66 +GVDLGGTT K+ I EG++ + T D ++ +I + Q + D D Sbjct: 6 IGVDLGGTTAKLAILTKEGDIEEKWTIDTNIEDKGAHIVKDIGDSINQKLTDLQLDNDIF 65 Query: 67 AGVGAGLAGFTNIREGIIILAPNIGF-RDVPIRSILEGRLNKPVKIDNDANVAALGEAWS 125 G+G G G N G + A N+G+ + + LE +++DNDANVAALGE W Sbjct: 66 YGIGMGTPGTVNYETGTVKGAYNLGWAEEQNVSEDLEKITGLKIELDNDANVAALGERWK 125 Query: 126 GAGRGVENCVCYTLXXXXXXXXXXXXKVYQGFAGLAGELGHISVVPDLEAIQCGCGNMGC 185 GAG G N V TL K+ G G AGE+GH++VVP+ C CG GC Sbjct: 126 GAGEGGANVVFVTLGTGVGGGIFAEGKILHGIRGAAGEIGHVTVVPE-NGYDCTCGKKGC 184 Query: 186 LETVSSATGIIRMANDAVSR--GD---RTSLSMVEKIAAKEVFDAAKAGDEVALRIVNRA 240 LETV+SATGI+R+A D G+ + +++ E I +K +F+ D++A +++ Sbjct: 185 LETVASATGIVRVAKDLAKEFTGESALKEAINKQETITSKLIFELGAENDKLANETIDKI 244 Query: 241 AFYLGKSMAAVAAVLNPEVFIVGGGVSKAGDILFDEVRRVFAKLAPAPLQTGVTIVPAEL 300 +FYL +++ + +LNPE I+GGGVS AGD L VR F + ++ + A Sbjct: 245 SFYLALALSHIGNMLNPEKIIIGGGVSAAGDQLLTPVRNYFETMVFPAVKESTKLSIATK 304 Query: 301 GNDAGIIGAAGLLL 314 GNDAGIIGAA L L Sbjct: 305 GNDAGIIGAAWLAL 318 Database: COG Posted date: Aug 3, 2010 9:58 AM Number of letters in database: 38,378,431 Number of sequences in database: 115,334 Lambda K H 0.319 0.138 0.401 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 115334 Number of Hits to DB: 23,145,751 Number of extensions: 967850 Number of successful extensions: 3026 Number of sequences better than 1.0e-05: 103 Number of HSP's gapped: 2778 Number of HSP's successfully gapped: 103 Length of query: 316 Length of database: 38,378,431 Length adjustment: 106 Effective length of query: 210 Effective length of database: 26,153,027 Effective search space: 5492135670 Effective search space used: 5492135670 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.4 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.7 bits) S2: 116 (49.3 bits)