BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0012.0011.p (212 letters) Database: COG 115,334 sequences; 38,378,431 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value BS_ycsK [E] COG2755 Lysophospholipase L1 and related esterases 122 5e-28 SPy1492 [E] COG2755 Lysophospholipase L1 and related esterases 83 4e-16 lin0749 [E] COG2755 Lysophospholipase L1 and related esterases 77 2e-14 L99502 [E] COG2755 Lysophospholipase L1 and related esterases 77 2e-14 lin1976 [E] COG2755 Lysophospholipase L1 and related esterases 75 6e-14 >BS_ycsK [E] COG2755 Lysophospholipase L1 and related esterases Length = 213 Score = 122 bits (305), Expect = 5e-28, Method: Compositional matrix adjust. Identities = 75/215 (34%), Positives = 113/215 (52%), Gaps = 9/215 (4%) Query: 1 MSYQYTAIGDSLTTGFGA-LPGNGFVPVYRRMAEGRLREPVGAVNLGINGLTTAALEQRL 59 M +YTA+GDSLTTG G+ L GFV + M E L+ +GL T E+ L Sbjct: 1 MVLRYTALGDSLTTGRGSGLFSPGFVQRFGDMMEADLKTRTAINIFARSGLNT---EEIL 57 Query: 60 G--SDPIFRQSIRNAEIITLSIGGNDLIKAAKAVSFRPGDLSPALARALRECKRNFGGIM 117 G S P ++ IR+A++IT++ GNDLI + ++++ +R C NF ++ Sbjct: 58 GLLSYPYVQKCIRDADMITITGCGNDLIDSV--LAYQTSKDETIFSRVSAHCHENFEKMI 115 Query: 118 GSLRQLKYGSRQPYIIRIVGLYNPYPQLTEATDWVRQFNAFAAGYSSGV-CGFASIYHEF 176 + ++K + PY IR+ LYNP+P++ A W+ FN+ +S A Y F Sbjct: 116 AKVAEIKGENPSPYAIRVFNLYNPFPEIDIAGKWITSFNSHLETLASAPHVKIADAYSIF 175 Query: 177 AGNERSLLSLDHVHPNGRGYRVIAGKLDSLGYWRL 211 G E+ LS D VHPN +GY+ +A + LGY L Sbjct: 176 KGKEQEYLSFDGVHPNSKGYQAMAEAVHKLGYKEL 210 >SPy1492 [E] COG2755 Lysophospholipase L1 and related esterases Length = 286 Score = 82.8 bits (203), Expect = 4e-16, Method: Compositional matrix adjust. Identities = 60/227 (26%), Positives = 104/227 (45%), Gaps = 33/227 (14%) Query: 1 MSYQYTAIGDSLTTGFGALPGNG-FVPVYRRMAEGRLREPVGAVNLGINGLTTAALEQRL 59 ++ QY AIGDSLT G G L G FVP+ + V N G++G T+ + R+ Sbjct: 52 VAIQYVAIGDSLTEGVGDLTHQGGFVPLLTNDLSEYFKANVNHQNYGVSGDTSQQILDRM 111 Query: 60 GSDPIFRQSIRNAEIITLSIGGNDLIKAAKAVSFRPGDLSPALARALRECKRNFGGIMGS 119 + S++ A+I+TL++GGND++ + +L+ + R+ R + + Sbjct: 112 IKQKQIQLSLKKADIMTLTVGGNDVMAVIRK------NLADLQVSSFRKPARQYQKRLRQ 165 Query: 120 LRQLKYGSRQPYIIRIVGLYNP----YPQLTEA----TDWVRQFNAFAAGYS-------- 163 + +L + I I+G+YNP +P+LT+ DW + Y Sbjct: 166 IIELARKDNKDLPIFILGIYNPFYLNFPELTDMQKVIDDWNTKTKEVVGEYDRVYFVPIN 225 Query: 164 ----SGVCGFASIYHEFAGNERS-----LLSLDHVHPNGRGYRVIAG 201 G+ G I H +G++ + L + DH HPN GY++++ Sbjct: 226 DLLYKGINGQEGIVHS-SGDQTTIVNDALFTGDHFHPNNTGYQIMSN 271 >lin0749 [E] COG2755 Lysophospholipase L1 and related esterases Length = 301 Score = 77.0 bits (188), Expect = 2e-14, Method: Compositional matrix adjust. Identities = 14/210 (6%), Positives = 34/210 (16%), Gaps = 20/210 (9%) Query: 2 SYQYTAIGDSLTTGFGALPGNGFVPVYRRMAEGRLREPVGAVNLGINGLTTAALEQRLGS 61 L + V + +L Sbjct: 56 YQAIYVDTPGLHMEEKRAINRLMNKAASSSIGDV-ELVIFVVEGTRWTPDDEMVLNKLRE 114 Query: 62 DPIFRQSIRNAEIITLSIGGNDLIKAAKAV-SFRPGDLSPALARALRECKRNFGGIMGSL 120 + ++ + + L + E N I + Sbjct: 115 GKAPVI-LAVNKVDNVQEKADLLPHLQFLASQMN----FLDIVPISAETGLNVDTIAAIV 169 Query: 121 RQLKYGSRQPYIIRIVGLYNPYPQLTEAT-------DWVRQFNAFAAGYSSGVCGFASIY 173 R+ + + Y + + + Sbjct: 170 RKHLPEATHHFP----EDYITDRSQRFMASEIIREKLMRFLGAELPYSVTVEI--ERFVS 223 Query: 174 HEFAGNERSLLSLDHVHPNGRGYRVIAGKL 203 +E G + + L L + G Sbjct: 224 NERGGYDINGLILVEREGQKKMVIGNKGAK 253 >L99502 [E] COG2755 Lysophospholipase L1 and related esterases Length = 288 Score = 76.6 bits (187), Expect = 2e-14, Method: Compositional matrix adjust. Identities = 58/225 (25%), Positives = 103/225 (45%), Gaps = 37/225 (16%) Query: 5 YTAIGDSLTTGFGALPGNG-FVPVYRRMAEGRLREPVGAVNLGINGLTTAALEQRLGSDP 63 Y A+GDSLT G G G G FVP++ + E + V + N G G T+ + R+ Sbjct: 59 YAALGDSLTEGVGDATGQGGFVPLFAKDIENKTDSSVSSQNFGKAGDTSTQIYNRMMKSK 118 Query: 64 IFRQSIRNAEIITLSIGGNDLIK-----AAKAVSFRPGDLSPALARALRECKRNFGGIMG 118 ++ A+IIT++IGGND++K +K S D + K+ ++ Sbjct: 119 KITDGLKKADIITITIGGNDVLKVIRDNVSKLSSMTEKDFTKPEELYQARVKK----LLD 174 Query: 119 SLRQLKYGSRQPYIIRIVGLYNP----YPQLTEATDWVRQFNAFAAGYSSG-----VCGF 169 +R+ ++ I ++G+YNP +P+LT + + +N AG S Sbjct: 175 KIREDNPKAQ----IYVLGIYNPFYLNFPELTVMQNVIDSWNTATAGVVSQEKNTYFIPI 230 Query: 170 ASIYHEFAGNERS--------------LLSLDHVHPNGRGYRVIA 200 + ++ +G++++ L + DH HPN GY+++A Sbjct: 231 NDLLYKGSGDKQAVESGSTSDAVSNNLLYTEDHFHPNNVGYQLMA 275 >lin1976 [E] COG2755 Lysophospholipase L1 and related esterases Length = 301 Score = 75.5 bits (184), Expect = 6e-14, Method: Compositional matrix adjust. Identities = 12/220 (5%), Positives = 31/220 (14%), Gaps = 35/220 (15%) Query: 7 AIGDSLTTGFGALPGNG----FVPVYRRMAEGRLREPVGAVNLGINGLTTAALEQRLGSD 62 G P E + + + L + Sbjct: 41 QTTRHRIVGIHTEGAYQAIYVDTP--GLHMEEKRAINRLMNKAASSSIGDVELVIFVVEG 98 Query: 63 PIFRQSIRNAEIITLSIGGNDL--IKAAKAVSFRPGDLSPALARALRECKRNFGGIMGSL 120 + + + V + L + + + Sbjct: 99 TRWTPDDEMVLNKLREGKAPVILAVNKVDNVQEKADLL----PHLQFLASQMNFLDIVPI 154 Query: 121 RQLKYGSRQPYIIRIVGLYNPYPQLTEATDWVRQFNAFAAGYSSGVCG---------FAS 171 + I + + EAT + + F Sbjct: 155 SAETGLNV----DTIAAIVRKHL--PEATH-HFPEDYITDRSQRFMASEIIREKLMRFLG 207 Query: 172 IYHEFAGNERS-------LLSLDHVHPNGRGYRVIAGKLD 204 ++ D + Sbjct: 208 AELPYSVTVEIERFVSNERGGYDINGLILVEREGQKKMVI 247 Database: COG Posted date: Aug 3, 2010 9:58 AM Number of letters in database: 38,378,431 Number of sequences in database: 115,334 Lambda K H 0.322 0.140 0.424 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 115334 Number of Hits to DB: 16,783,481 Number of extensions: 698393 Number of successful extensions: 1737 Number of sequences better than 1.0e-05: 9 Number of HSP's gapped: 1725 Number of HSP's successfully gapped: 9 Length of query: 212 Length of database: 38,378,431 Length adjustment: 102 Effective length of query: 110 Effective length of database: 26,614,363 Effective search space: 2927579930 Effective search space used: 2927579930 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.4 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.9 bits) S2: 113 (48.1 bits)