BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0012.0008.p (336 letters) Database: COG 115,334 sequences; 38,378,431 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value NMA0246 [G] COG0057 Glyceraldehyde-3-phosphate dehydrogenase/ery... 532 e-151 SA0727 [G] COG0057 Glyceraldehyde-3-phosphate dehydrogenase/eryt... 528 e-150 NMB2159 [G] COG0057 Glyceraldehyde-3-phosphate dehydrogenase/ery... 527 e-150 L0005 [G] COG0057 Glyceraldehyde-3-phosphate dehydrogenase/eryth... 500 e-141 L0004 [G] COG0057 Glyceraldehyde-3-phosphate dehydrogenase/eryth... 477 e-135 >NMA0246 [G] COG0057 Glyceraldehyde-3-phosphate dehydrogenase/erythrose-4-phosphate dehydrogenase Length = 422 Score = 532 bits (1371), Expect = e-151, Method: Compositional matrix adjust. Identities = 30/332 (9%), Positives = 56/332 (16%), Gaps = 1/332 (0%) Query: 1 MSVKVGINGFGRIGRLAFRRIQNVEGIEVVAINDLTDAKMLAHLLKYDTTQGKFQGEVEV 60 M+ + I+ G+L E I T A +L H + G + Sbjct: 1 MTYRDFIDQLRENGKLVEVPQPVSPRFEASRIAKKTKAPVLFHDISGSKVIMNLLGSRDE 60 Query: 61 HDGFFKVNGKEVKVLANRNPEELPWGDLGVDIVLECTGFFTTKEAAEKHLKGGAKKVVIS 120 V +E+ + E + LE Sbjct: 61 LASMLGVPKEEIIRKLSEVSPEGEVRIVSESPTLEVIENEVDLTRLPILTHYEKDGAPYI 120 Query: 121 APATGDMKTVVYNVNHDVLDGTETVISGASCTTNCLAPMAKVLNDKFGIVEGLMTTIHAY 180 + A+ + + Sbjct: 121 TAGIVVSEYGGVMNASIHRLMLAGKDKLAARLVPPRHTYLLHKKAVEKGEPLPVAIVIGC 180 Query: 181 TGDQNTLDAPHAKGDFRRARAAAENIVPNTTGAAKAIGLVIPALKGKLDGAAQRVPVATG 240 AAA P V + V G Sbjct: 181 DPTITYATTTRVPVGKEFEYAAALRGAPVELFECSNGVKVPHSEIVLEGYVDPVEKVDEG 240 Query: 241 SLTELVTVLEKNVTVEELNAAMKAASDPDTYGYTEDEIVSSDIKGMTFGSLFDATQTKVM 300 ++ + + D + + M + Sbjct: 241 PFVDITGTYDMVRKEPVIRITRVLHRK-DPIYHGILPAGPEHLLMMGVPYEPRIYRAVGE 299 Query: 301 TVGDKQLVKTVAWYDNEMSYTAQLVRTLEHFA 332 K +V T + +T Sbjct: 300 VTTVKNVVLTEGGCCYLHAVVQIKKQTEGDAK 331 >SA0727 [G] COG0057 Glyceraldehyde-3-phosphate dehydrogenase/erythrose-4-phosphate dehydrogenase Length = 336 Score = 528 bits (1359), Expect = e-150, Method: Compositional matrix adjust. Identities = 253/337 (75%), Positives = 294/337 (87%), Gaps = 2/337 (0%) Query: 1 MSVKVGINGFGRIGRLAFRRIQNVEGIEVVAINDLTDAKMLAHLLKYDTTQGKFQGEVEV 60 M+VKV INGFGRIGRLAFRRIQ VEG+EVVA+NDLTD MLAHLLKYDT QG+F GEVEV Sbjct: 1 MAVKVAINGFGRIGRLAFRRIQEVEGLEVVAVNDLTDDDMLAHLLKYDTMQGRFTGEVEV 60 Query: 61 HDGFFKVNGKEVKVLANRNPEELPWGDLGVDIVLECTGFFTTKEAAEKHLKGGAKKVVIS 120 DG F+VNGKEVK + + +LPW DL +D+VLECTGF+T K+ A+ H++ GAKKV+IS Sbjct: 61 VDGGFRVNGKEVKSFSEPDASKLPWKDLNIDVVLECTGFYTDKDKAQAHIEAGAKKVLIS 120 Query: 121 APATGDMKTVVYNVNHDVLDGTETVISGASCTTNCLAPMAKVLNDKFGIVEGLMTTIHAY 180 APATGD+KT+V+N NH LDG+ETV+SGASCTTN LAP+AKVLND FG+VEGLMTTIHAY Sbjct: 121 APATGDLKTIVFNTNHQELDGSETVVSGASCTTNSLAPVAKVLNDDFGLVEGLMTTIHAY 180 Query: 181 TGDQNTLDAPHAKGDFRRARAAAENIVPNTTGAAKAIGLVIPALKGKLDGAAQRVPVATG 240 TGDQNT DAPH KGD RRARAAAENI+PN+TGAAKAIG VIP + GKLDG AQRVPVATG Sbjct: 181 TGDQNTQDAPHRKGDKRRARAAAENIIPNSTGAAKAIGKVIPEIDGKLDGGAQRVPVATG 240 Query: 241 SLTELVTVLEK-NVTVEELNAAMKAASDPDTYGYTEDEIVSSDIKGMTFGSLFDATQTKV 299 SLTEL VLEK +VTVE++N AMK AS+ +++GYTEDEIVSSD+ GMT+GSLFDATQT+V Sbjct: 241 SLTELTVVLEKQDVTVEQVNEAMKNASN-ESFGYTEDEIVSSDVVGMTYGSLFDATQTRV 299 Query: 300 MTVGDKQLVKTVAWYDNEMSYTAQLVRTLEHFAKLAK 336 M+VGD+QLVK AWYDNEMSYTAQLVRTL + A+L+K Sbjct: 300 MSVGDRQLVKVAAWYDNEMSYTAQLVRTLAYLAELSK 336 >NMB2159 [G] COG0057 Glyceraldehyde-3-phosphate dehydrogenase/erythrose-4-phosphate dehydrogenase Length = 334 Score = 527 bits (1358), Expect = e-150, Method: Compositional matrix adjust. Identities = 250/332 (75%), Positives = 286/332 (86%), Gaps = 1/332 (0%) Query: 1 MSVKVGINGFGRIGRLAFRRIQNVEGIEVVAINDLTDAKMLAHLLKYDTTQGKFQGEVEV 60 MS+KV INGFGRIGRLA R+I+ IEVVA+NDLT A+ML HL KYD+TQG+FQG E+ Sbjct: 1 MSIKVAINGFGRIGRLALRQIEKAHDIEVVAVNDLTPAEMLLHLFKYDSTQGRFQGTAEL 60 Query: 61 HDGFFKVNGKEVKVLANRNPEELPWGDLGVDIVLECTGFFTTKEAAEKHLKGGAKKVVIS 120 D VNGKE+KV AN NPEELPWG+LGVD++LECTGFFT K AE H++ GA+KVVIS Sbjct: 61 KDDAIVVNGKEIKVFANPNPEELPWGELGVDVILECTGFFTNKTKAEAHIRAGARKVVIS 120 Query: 121 APATGDMKTVVYNVNHDVLDGTETVISGASCTTNCLAPMAKVLNDKFGIVEGLMTTIHAY 180 AP D+KTVVY VN D+LDG+ETVIS ASCTTNCLAPMA VL +FG+VEGLMTTIHAY Sbjct: 121 APGGNDVKTVVYGVNQDILDGSETVISAASCTTNCLAPMAAVLQKEFGVVEGLMTTIHAY 180 Query: 181 TGDQNTLDAPHAKGDFRRARAAAENIVPNTTGAAKAIGLVIPALKGKLDGAAQRVPVATG 240 TGDQNTLDAPH KGD RRARAAA NIVPN+TGAAKAIGLVIP L GKLDG+AQRVPVA+G Sbjct: 181 TGDQNTLDAPHRKGDLRRARAAALNIVPNSTGAAKAIGLVIPELNGKLDGSAQRVPVASG 240 Query: 241 SLTELVTVLEKNVTVEELNAAMKAASDPDTYGYTEDEIVSSDIKGMTFGSLFDATQTKVM 300 SLTELV++LE+ VT EE+NAAMKAA+ ++YGY ED+IVSSD+ G+ +GSLFDATQT+VM Sbjct: 241 SLTELVSILERPVTKEEINAAMKAAAS-ESYGYNEDQIVSSDVVGIEYGSLFDATQTRVM 299 Query: 301 TVGDKQLVKTVAWYDNEMSYTAQLVRTLEHFA 332 TVG KQLVKTVAWYDNEMSYT QLVRTLE+FA Sbjct: 300 TVGGKQLVKTVAWYDNEMSYTCQLVRTLEYFA 331 >L0005 [G] COG0057 Glyceraldehyde-3-phosphate dehydrogenase/erythrose-4-phosphate dehydrogenase Length = 336 Score = 500 bits (1288), Expect = e-141, Method: Compositional matrix adjust. Identities = 246/337 (72%), Positives = 281/337 (83%), Gaps = 2/337 (0%) Query: 1 MSVKVGINGFGRIGRLAFRRIQNVEGIEVVAINDLTDAKMLAHLLKYDTTQGKFQGEVEV 60 M VKVGINGFGRIGRLAFRRIQNVEG+EVVAINDLTD MLAHLLKYDTTQG+F G+VEV Sbjct: 1 MVVKVGINGFGRIGRLAFRRIQNVEGVEVVAINDLTDPAMLAHLLKYDTTQGRFDGKVEV 60 Query: 61 HDGFFKVNGKEVKVLANRNPEELPWGDLGVDIVLECTGFFTTKEAAEKHLKG-GAKKVVI 119 DG F+VNGK VKV A NP + W ++G +IVLE TGFF TKE AE+HL GAKKVVI Sbjct: 61 KDGGFEVNGKFVKVTAESNPANINWAEVGAEIVLEATGFFATKEKAEQHLHANGAKKVVI 120 Query: 120 SAPATGDMKTVVYNVNHDVLDGTETVISGASCTTNCLAPMAKVLNDKFGIVEGLMTTIHA 179 +AP D+KT+V+N NH+VLDGTETVIS SCTTNCLAPMA LN +FGI G MTT+H Sbjct: 121 TAPGGSDVKTIVFNTNHEVLDGTETVISAGSCTTNCLAPMADTLNKQFGIKVGTMTTVHG 180 Query: 180 YTGDQNTLDAPHAKGDFRRARAAAENIVPNTTGAAKAIGLVIPALKGKLDGAAQRVPVAT 239 YTGDQ TLD PH GDFRRARAAAENIVPN+TGAAKAIGLV+P L+GKL G AQRVPV T Sbjct: 181 YTGDQMTLDGPHRGGDFRRARAAAENIVPNSTGAAKAIGLVLPELQGKLQGHAQRVPVPT 240 Query: 240 GSLTELVTVLEKNVTVEELNAAMKAASDPDTYGYTEDEIVSSDIKGMTFGSLFDATQTKV 299 GSLTELVT+L K VTV+E+NAAMKAAS+ +++GY ED+IVSSDI G++ SLFDATQT+V Sbjct: 241 GSLTELVTILNKEVTVDEINAAMKAASN-ESFGYNEDQIVSSDIVGISNSSLFDATQTEV 299 Query: 300 MTVGDKQLVKTVAWYDNEMSYTAQLVRTLEHFAKLAK 336 + QLVKTV+WYDNEMSYT+ LVRTL +FAK+AK Sbjct: 300 TSADGAQLVKTVSWYDNEMSYTSNLVRTLAYFAKIAK 336 >L0004 [G] COG0057 Glyceraldehyde-3-phosphate dehydrogenase/erythrose-4-phosphate dehydrogenase Length = 337 Score = 477 bits (1228), Expect = e-135, Method: Compositional matrix adjust. Identities = 240/338 (71%), Positives = 274/338 (81%), Gaps = 3/338 (0%) Query: 1 MSVKVGINGFGRIGRLAFRRIQNVEGIEVVAINDLTDAKMLAHLLKYDTTQGKFQGEVEV 60 M VKVGINGFGRIGRLA RRIQ VEG+EV INDLTD MLAHLLKYDTTQG+F+G VEV Sbjct: 1 MVVKVGINGFGRIGRLALRRIQEVEGVEVAHINDLTDPAMLAHLLKYDTTQGRFKGTVEV 60 Query: 61 HDGFFKVNGKEVKVLANRNPEELPWGDLGVDIVLECTGFFTTKEAAEKHLK-GGAKKVVI 119 + F VNGK VKV A RNPE++ W D GV+IVLE TGFF TKE AEKHL GGAKKV+I Sbjct: 61 KEDGFDVNGKFVKVTAERNPEDIQWADSGVEIVLEATGFFATKEKAEKHLHPGGAKKVLI 120 Query: 120 SAPATGDMKTVVYNVNHDVLDGTETVISGASCTTNCLAPMAKVLNDKFGIVEGLMTTIHA 179 +AP D+KTVV+N NH +LDGTETVIS SCTTN LAPMA LN FG+ G MTT+H+ Sbjct: 121 TAPGGNDVKTVVFNTNHTILDGTETVISAGSCTTNSLAPMADALNKNFGVKGGTMTTVHS 180 Query: 180 YTGDQNTLDAPHAKGDFRRARAAAENIVPNTTGAAKAIGLVIPALKGKLDGAAQRVPVAT 239 YTGDQ TLD PH GDFRRARAAAENIVP ++GAAKAIGLV+P L G + G AQRV T Sbjct: 181 YTGDQMTLDGPHRGGDFRRARAAAENIVPASSGAAKAIGLVLPELSGLMKGHAQRVSTPT 240 Query: 240 GSLTELVTVLEKNVTVEELNAAMKAASDPDTYGYTEDEIVSSDIKGMTFGSLFDATQTKV 299 GS+TELVTVLEK+VTV+E+N AMKAA+D +++GY DEIVSSDI GM +GSLFDAT T+V Sbjct: 241 GSITELVTVLEKHVTVDEINEAMKAAAD-ESFGYNVDEIVSSDIIGMAYGSLFDATLTEV 299 Query: 300 MTVGD-KQLVKTVAWYDNEMSYTAQLVRTLEHFAKLAK 336 + D QLVKT AWYDNEMS+TAQL+RTLE+FAK+AK Sbjct: 300 TDLKDGGQLVKTAAWYDNEMSFTAQLIRTLEYFAKIAK 337 Database: COG Posted date: Aug 3, 2010 9:58 AM Number of letters in database: 38,378,431 Number of sequences in database: 115,334 Lambda K H 0.316 0.132 0.375 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 115334 Number of Hits to DB: 24,421,364 Number of extensions: 1022569 Number of successful extensions: 2559 Number of sequences better than 1.0e-05: 52 Number of HSP's gapped: 2343 Number of HSP's successfully gapped: 52 Length of query: 336 Length of database: 38,378,431 Length adjustment: 107 Effective length of query: 229 Effective length of database: 26,037,693 Effective search space: 5962631697 Effective search space used: 5962631697 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.3 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.6 bits) S2: 116 (49.3 bits)