BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0011.0001.p (547 letters) Database: COG 115,334 sequences; 38,378,431 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value BH3808 [J] COG0018 Arginyl-tRNA synthetase 679 0.0 YDR341c [J] COG0018 Arginyl-tRNA synthetase 119 1e-26 BH0834 [J] COG0018 Arginyl-tRNA synthetase 112 1e-24 YHR091c [J] COG0018 Arginyl-tRNA synthetase 98 4e-20 >BH3808 [J] COG0018 Arginyl-tRNA synthetase Length = 346 Score = 679 bits (1751), Expect = 0.0, Method: Compositional matrix adjust. Identities = 0/548 (0%), Positives = 0/548 (0%), Gaps = 3/548 (0%) Query: 1 LREXXXXXXXXXXXXTREELPAIVLEVPKDKAHGDLATNAAMQLTKIAKRNPRQIAEAII 60 L E A +L A M + Sbjct: 11 LEVSTLGLGTMTFGEQNSEADAHAQLDYAVAQGINLIDVAEMYPVPPRPETQGLTETYVG 70 Query: 61 GHLDKNAASIEKAEIAGPGFINFTLSKSYLYPVIALVAEQGDNYGRIDAGQGQRVEMEFV 120 L K+ + + + + K + + Sbjct: 71 NWLAKHGSREKLIIASKVSGPSRNNDKGIRPDQALDRKNIREALHDSLKRLQTDYLDLYQ 130 Query: 121 SANPTGSLHLGHARGAAVGDALCNVLDFAGYEVTREYYINDAGNQVANLCKSIEARYLQE 180 P + G + D+ V + EY + ++ Sbjct: 131 VHWPQRPTNCFGKLGYSWTDSAPAVSLLDTLDALAEYQRAGKIRYIGVSNETAFGVMRYL 190 Query: 181 LGQPAEMPEDGYHGEDIKGFAKELVAEKGDALLSMTPGDRAAFFRTYGLTKELDKIKRDL 240 ++ + + A+ T + Sbjct: 191 HLADKHDLPRIVTIQNPYSLLNRSFEVGLAEVSQYEGVELLAYSCLGFGTLTGKYLNGAK 250 Query: 241 TRFRVGFDIWFSETSLYENGEVLRSLDELRERGEVYEQDGATWLQTTKYGDDKDRVLIKN 300 E + + R + + +L Sbjct: 251 PAGARNTLFSRFTRYSGEQTQKAVAAYVDIARRHGLDPAQMALAFVRRQPFVASTLLGAT 310 Query: 301 DGTYTYLTPDIAYHNDKYGRGYDTMINIWGADHHGYIPRMKAAMSALGNDPEKLVVLIAQ 360 Sbjct: 311 370 Query: 361 MVSLFQNGEKVKMSKRTGKAVTMEDLMDEVGIDAIRYFFTMRSMDSHLDFDMDLAISTSN 420 Sbjct: 371 430 Query: 421 ENPVFYVQYAHARICSIFRQAEEQGIVLPDVAEIDYAKLTAEHEYALLRKIGELPAEIGI 480 Sbjct: 431 -- 488 Query: 481 AASSYAPHRLIRYVYDLASLFHSYYKAERVIT-EDXXXXXXXXXXXXXXXXXXXNVLRLV 539 Sbjct: 489 548 Query: 540 GVTAPDRM 547 Sbjct: 549 556 >YDR341c [J] COG0018 Arginyl-tRNA synthetase Length = 346 Score = 119 bits (299), Expect = 1e-26, Method: Compositional matrix adjust. Identities = 0/529 (0%), Positives = 0/529 (0%), Gaps = 72/529 (13%) Query: 70 IEKAEIAGPGFINFTLSKSYLYP-VIALVAEQGDNYGRIDAGQGQRVEMEFVSANPTGSL 128 + I L S L Q R G+ SA L Sbjct: 100 RPDQALDRK-NIREALHDSLKRLQTDYLDLYQVHWPQRPTNCFGKLGYSWTDSAPAVSLL 158 Query: 129 HLGHARGAAVGDALCNVLDFAGYEVTREYYINDAGNQVANLCKSIEARYLQELGQPAEMP 188 A + + ++ + Sbjct: 159 DTLDALAEYQRAGKIRYIGVSNETAFGVMRYLHLADKHDLPRIVTI-QNPYS---LLNRS 214 Query: 189 EDGYHGEDIKGFAKELVAEKGDAL--------------LSMTPGDRAAF-----FRTYGL 229 + E + EL+A + + Sbjct: 215 FEVGLAEVSQYEGVELLAYSCLGFGTLTGKYLNGAKPAGARNTLFSRFTRYSGEQTQKAV 274 Query: 230 TKELDKIKRDLTRFRVGFDIWFSETSLYENGEVLRSLDELRERGEVYEQDGATWLQTTKY 289 +D R + + V +L ++ + L+ ++ Sbjct: 275 AAYVDI----ARRHGLDPAQMALAFVRRQPF-VASTLLGATTMDQLKTNIESLHLELSED 329 Query: 290 GDDKDRVLI-KNDGTYTYLTPDIAYHNDKYGR-GYDTMINIWGADHHGYIPRMKAAMSAL 347 + + Sbjct: 330 VLAEIEAVHQ 389 Query: 348 GNDPEKLVVLIAQMVSLFQNGEKVKMSKRTGKAVTMEDLMDE------------------ 389 Sbjct: 390 ------- 442 Query: 390 ----------VGIDAIRYFFTMRSMDSHLDFDMDLAISTSNEN-PVFYVQYAHARICSIF 438 Sbjct: 443 -- 500 Query: 439 RQAEEQGIVLPDVAEIDYAKLTAEHEYALLRKIGELPAEIGIAASSYAPHRLIRYVYDLA 498 Sbjct: 501 -- 558 Query: 499 SLFHSYYKAERVITEDXXXXXXXXXXXXXXXXXXXNVLRLVGVTAPDRM 547 Sbjct: 559 607 >BH0834 [J] COG0018 Arginyl-tRNA synthetase Length = 346 Score = 112 bits (281), Expect = 1e-24, Method: Compositional matrix adjust. Identities = 0/573 (0%), Positives = 0/573 (0%), Gaps = 84/573 (14%) Query: 24 VLEVPKDKAHGDLATNAAMQLTKIAKRNPRQIAEAIIGHLDKNAASIEKAEI---AGPGF 80 + + AH L A Q + Sbjct: 24 GEQNSEADAHAQLDY-AVAQGINLIDVAEMYPVPPR------PETQGLTETYVGNWLAKH 76 Query: 81 INFTLSKSYLYPVIALVAEQGDN-YGRIDAGQGQRVEMEFVSANPTGSLHLGHARGAAVG 139 + ++ + D A + + + Sbjct: 77 GSREKLIIAS--KVSGPSRNNDKGIRPDQALDRKNIREALHDSLKRLQTDYLDLYQVHWP 134 Query: 140 DALCNVLDFAGYEVTREYYINDAGNQVANLCKSIEARYLQELGQPAEMPEDG-------- 191 N GY T + + E + + Sbjct: 135 QRPTNCFGKLGYSWTDSAPAVSLLDTLD---ALAEY--QRAGKIRYIGVSNETAFGVMRY 189 Query: 192 --YHGEDIKGFAKELVAEKGDALLSMTPGDRAA-FFRTYGLTKELDKIKRDLTRFRVGFD 248 + + + + +LL+ + A + G+ T + Sbjct: 190 LHLADKHDLP--RIVTIQNPYSLLNRSFEVGLAEVSQYEGVELLAYSCLGFGTLTGKYLN 247 Query: 249 IWFSETSLYENGEVLRSLDELRERGEVYEQDGATWLQTTKYGDDKDRVLIKNDGTYTYLT 308 N R E+ + + D L + Sbjct: 248 GAKPAG--ARNTLFSRFTRYSGEQTQKAVAAYVD-IARRHGLDPAQMALAFVRRQPFVAS 304 Query: 309 PDIAY----HNDKYGRGYDTMINIWGADHHGYIPRMKAAMSALG----NDPEKLVVLIAQ 360 + +++ D I + + Sbjct: 305 TLLGATTMDQLKTNI---ESLHLELSEDVLAEIEAVHQVYTYPA 361 Query: 361 MVSLFQNGEKVKMSKRTGKAVTMEDLMDE-------------------------VGIDAI 395 Sbjct: 362 ------- 414 Query: 396 RYFFTMRSMDSHLDFDMDLAISTSNENPVF-YVQYAHARICSIFRQAEEQGIVLPDVAEI 454 Sbjct: 415 ------ 468 Query: 455 DYAKLTAEHEYALLRKIGELPAEIGIAASSYAPHRLIRYVYDLASLFHSYYKAERVITED 514 Sbjct: 469 528 Query: 515 XXXXXXXXXXXXXXXXXXXNVLRLVGVTAPDRM 547 Sbjct: 529 561 >YHR091c [J] COG0018 Arginyl-tRNA synthetase Length = 346 Score = 97.8 bits (242), Expect = 4e-20, Method: Compositional matrix adjust. Identities = 0/576 (0%), Positives = 0/576 (0%), Gaps = 81/576 (14%) Query: 25 LEVPKDKAHGDLATNAAMQLTKIAKRNPRQIAEAIIGHLDKNAASIEKAEIAGPGFINFT 84 + + D + R + + + ++T Sbjct: 96 DKGIRPDQALDRK----NIREALHDSLKRLQTDYLDLYQVHWPQ-RPTNCFGKL-GYSWT 149 Query: 85 LSKSYLY-PVIALVAEQGDNYGRIDAGQGQRVEMEFVSANPTGSLHLGHARGAAVGDALC 143 S + + G+I V + R + + Sbjct: 150 DSAPAVSLLDTLDALAEYQRAGKIRYIGVSNETAFGVMRYLHLADKHDLPRIVTIQNPYS 209 Query: 144 NVLDFAGYEVTREYYINDAGNQVANLCKSI----EARYLQELGQPAEMPEDGY------- 192 + + + + + Sbjct: 210 LLNRSFEVGLAEVSQYEGVELLAYSCLGFGTLTGKYLNGAK--PAGARNTLFSRFTRYSG 267 Query: 193 HGEDIKGFAKELVAEKGDALL-SMTPGDRAAF-----FRTYGLTKELDKIKRDLTRFRVG 246 A +A + M T + + Sbjct: 268 EQTQKAVAAYVDIARRHGLDPAQMALAFVRRQPFVASTLLGATTMDQ----LKTNIESLH 323 Query: 247 FDIWFSETSLYENGEVLRSLDELRERGEVYEQDGATWLQTTKYGDDKDRVLI-KNDGTYT 305 ++ Sbjct: 324 LEL- 382 Query: 306 YLTPDI-AYHNDKYGRGYDTMINIWGADHHGYIPRMKAAMSALGNDPEKLVVLIAQMVSL 364 Sbjct: 383 ------- 435 Query: 365 FQNGEKVKMSKRTGKAVTMEDLMDE----------------------------VGIDAIR 396 Sbjct: 436 - 494 Query: 397 YFFTMRSMDS----HLDFDMDLAISTSNEN-PVFYVQYAHARICSIFRQAEEQGIVLPDV 451 Sbjct: 495 ------- 547 Query: 452 AEIDYAKLTAEHEYALLRKIGELPAEIGIAASSYAPHRLIRYVYDLASLFHSYYKAERVI 511 Sbjct: 548 607 Query: 512 TEDXXXXXXXXXXXXXXXXXXXNVLRLVGVTAPDRM 547 Sbjct: 608 643 Database: COG Posted date: Aug 3, 2010 9:58 AM Number of letters in database: 38,378,431 Number of sequences in database: 115,334 Lambda K H 0.318 0.136 0.393 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 115334 Number of Hits to DB: 39,683,333 Number of extensions: 1698882 Number of successful extensions: 4010 Number of sequences better than 1.0e-05: 4 Number of HSP's gapped: 3997 Number of HSP's successfully gapped: 4 Length of query: 547 Length of database: 38,378,431 Length adjustment: 111 Effective length of query: 436 Effective length of database: 25,576,357 Effective search space: 11151291652 Effective search space used: 11151291652 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.3 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.7 bits) S2: 118 (50.1 bits)