BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0007.0004.p (634 letters) Database: COG 115,334 sequences; 38,378,431 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value BS_gyrB [L] COG0187 Type IIA topoisomerase (DNA gyrase/topo II, ... 843 0.0 BH0006 [L] COG0187 Type IIA topoisomerase (DNA gyrase/topo II, t... 838 0.0 lin0006 [L] COG0187 Type IIA topoisomerase (DNA gyrase/topo II, ... 780 0.0 CAC0006 [L] COG0187 Type IIA topoisomerase (DNA gyrase/topo II, ... 759 0.0 L0283 [L] COG0187 Type IIA topoisomerase (DNA gyrase/topo II, to... 743 0.0 >BS_gyrB [L] COG0187 Type IIA topoisomerase (DNA gyrase/topo II, topoisomerase IV), B subunit Length = 638 Score = 843 bits (2178), Expect = 0.0, Method: Compositional matrix adjust. Identities = 404/634 (63%), Positives = 494/634 (77%), Gaps = 2/634 (0%) Query: 3 QPTYDESQIQVLEGLEAVRKRPGMYIGSTSAKGLHHLVWEVVDNSIDEALAGYCDRIQVK 62 Q +YDE+QIQVLEGLEAVRKRPGMYIGST++KGLHHLVWE+VDNSIDEALAGYC I ++ Sbjct: 5 QNSYDENQIQVLEGLEAVRKRPGMYIGSTNSKGLHHLVWEIVDNSIDEALAGYCTDINIQ 64 Query: 63 IHEDNSVTVIDNGRGIPVGENVKLKKSTLEVVMTVLHAXXXXXXXXXXXXXXXXXXXISV 122 I +DNS+TV+DNGRGIPVG + K+ + +EV+MTVLHA SV Sbjct: 65 IEKDNSITVVDNGRGIPVGIHEKMGRPAVEVIMTVLHAGGKFDGSGYKVSGGLHGVGASV 124 Query: 123 VNALSEKVVVNVKRDGHIYQQEYRRGAPQYDIKIVXXXXXXXXXXXFLPDPEIFTETTVF 182 VNALS ++ V V RDG I++Q Y+RG P D++I+ F+PDPEIF+ETT + Sbjct: 125 VNALSTELDVTVHRDGKIHRQTYKRGVPVTDLEIIGETDHTGTTTHFVPDPEIFSETTEY 184 Query: 183 EYTTLLTRIRELAFLNKGIELSLLDERTGV--SNVFKYEGGIVEYVKYLNEKKEALHEEP 240 +Y L R+RELAFL KG+ +++ D+R G N + YEGGI YV+YLN KE +HEEP Sbjct: 185 DYDLLANRVRELAFLTKGVNITIEDKREGQERKNEYHYEGGIKSYVEYLNRSKEVVHEEP 244 Query: 241 IYVEGSRDMIAVEVALQYNDSYTENIYSFANNINTHEGGTHESGFKSALTRIINDYARKT 300 IY+EG +D I VEVALQYNDSYT NIYSF NNINT+EGGTHE+GFK+ LTR+INDYARK Sbjct: 245 IYIEGEKDGITVEVALQYNDSYTSNIYSFTNNINTYEGGTHEAGFKTGLTRVINDYARKK 304 Query: 301 GVIKDSSSNLTGDDVREGLTAIISVKIPEPQFEGQTKTKLGNSEVRGVVESLFGEKLQEF 360 G+IK++ NL+GDDVREGLTAIIS+K P+PQFEGQTKTKLGNSE R + ++LF ++ F Sbjct: 305 GLIKENDPNLSGDDVREGLTAIISIKHPDPQFEGQTKTKLGNSEARTITDTLFSTAMETF 364 Query: 361 LEENPAVSRRVLEKSMQXXXXXXXXXXXXXXXXXXSALEVSALPGKLADCSSKDASISEL 420 + ENP ++++++K + SALE+S LPGKLADCSSKD SISEL Sbjct: 365 MLENPDAAKKIVDKGLMAARARMAAKKARELTRRKSALEISNLPGKLADCSSKDPSISEL 424 Query: 421 YIVEGDSAGGSAKQGRDRHFQAILPLRGKILNVEKARLDRILSNAEIRAIITALGTGISD 480 YIVEGDSAGGSAKQGRDRHFQAILPLRGKILNVEKARLD+ILSN E+R++ITALGTGI + Sbjct: 425 YIVEGDSAGGSAKQGRDRHFQAILPLRGKILNVEKARLDKILSNNEVRSMITALGTGIGE 484 Query: 481 DFDLSKARYHKVVIMTDADVDGAHIRTLLLTFFYRYMRKIIEAGYIYIAQPPLFKIERNK 540 DF+L KARYHKVVIMTDADVDGAHIRTLLLTFFYRYMR+IIE GY+YIAQPPL+K+++ K Sbjct: 485 DFNLEKARYHKVVIMTDADVDGAHIRTLLLTFFYRYMRQIIENGYVYIAQPPLYKVQQGK 544 Query: 541 VVRYAQTEKERDEIIASFGENVKVNVQRYKGLGEMNAAQLWDTTMDPESRMMLQVTIEDA 600 V YA +KE +E++ + + K +QRYKGLGEMNA QLW+TTMDP SR +LQVT+EDA Sbjct: 545 RVEYAYNDKELEELLKTLPQTPKPGLQRYKGLGEMNATQLWETTMDPSSRTLLQVTLEDA 604 Query: 601 ILADGIFDTLMGDNVEPRREFIQEHAQSVRNLDI 634 + AD F+ LMGD VEPRR FI+ +A+ V+NLDI Sbjct: 605 MDADETFEMLMGDKVEPRRNFIEANARYVKNLDI 638 >BH0006 [L] COG0187 Type IIA topoisomerase (DNA gyrase/topo II, topoisomerase IV), B subunit Length = 637 Score = 838 bits (2165), Expect = 0.0, Method: Compositional matrix adjust. Identities = 403/633 (63%), Positives = 489/633 (77%), Gaps = 1/633 (0%) Query: 3 QPTYDESQIQVLEGLEAVRKRPGMYIGSTSAKGLHHLVWEVVDNSIDEALAGYCDRIQVK 62 Q YDESQIQVLEGLEAVRKRPGMYIGSTS++GLHHLVWE+VDNSIDEA+AG+CD I V Sbjct: 5 QQAYDESQIQVLEGLEAVRKRPGMYIGSTSSRGLHHLVWEIVDNSIDEAMAGFCDEIGVV 64 Query: 63 IHEDNSVTVIDNGRGIPVGENVKLKKSTLEVVMTVLHAXXXXXXXXXXXXXXXXXXXISV 122 I EDNS+TV DNGRGIPVG + K+ + +EV+MTVLHA SV Sbjct: 65 IEEDNSITVTDNGRGIPVGIHEKMGRPAVEVIMTVLHAGGKFGGGGYKVSGGLHGVGASV 124 Query: 123 VNALSEKVVVNVKRDGHIYQQEYRRGAPQYDIKIVXXXXXXXXXXXFLPDPEIFTETTVF 182 VNALS + V V R+G ++ Q++ RG P D++++ F PD +IFTETTVF Sbjct: 125 VNALSTMLEVEVHREGKVHYQKFHRGVPAADLEVIGTTDRTGTKIHFKPDGDIFTETTVF 184 Query: 183 EYTTLLTRIRELAFLNKGIELSLLDERTG-VSNVFKYEGGIVEYVKYLNEKKEALHEEPI 241 EY TL +R+RELAFLNKG+ + + D R S+ F YEGGI +V++LN +E LHE PI Sbjct: 185 EYDTLASRLRELAFLNKGLRIKITDMREEEKSDEFHYEGGIASFVEHLNRTRETLHETPI 244 Query: 242 YVEGSRDMIAVEVALQYNDSYTENIYSFANNINTHEGGTHESGFKSALTRIINDYARKTG 301 ++EG + + VE+A+QYND +T NIYSFANNINTHEGGTHESGFK+ LTR+INDYARK Sbjct: 245 HIEGEKQGVYVEIAVQYNDGFTSNIYSFANNINTHEGGTHESGFKTGLTRVINDYARKHN 304 Query: 302 VIKDSSSNLTGDDVREGLTAIISVKIPEPQFEGQTKTKLGNSEVRGVVESLFGEKLQEFL 361 + K+S NLTG+DVREGLTAIISVKIP+PQFEGQTKTKLGNSE R + +SLF E FL Sbjct: 305 LFKESDPNLTGEDVREGLTAIISVKIPDPQFEGQTKTKLGNSEARTITDSLFTEHFSRFL 364 Query: 362 EENPAVSRRVLEKSMQXXXXXXXXXXXXXXXXXXSALEVSALPGKLADCSSKDASISELY 421 E+P V+R++++K + SALEVS+LPGKLADCSS+DASISE+Y Sbjct: 365 IEHPQVARKLVDKGLMASRAREAAKKARELTRRKSALEVSSLPGKLADCSSRDASISEIY 424 Query: 422 IVEGDSAGGSAKQGRDRHFQAILPLRGKILNVEKARLDRILSNAEIRAIITALGTGISDD 481 IVEGDSAGGSAKQGRDRHFQAILPLRGKILNVEKARLD+IL+N EIRAIITALGTGI DD Sbjct: 425 IVEGDSAGGSAKQGRDRHFQAILPLRGKILNVEKARLDKILANNEIRAIITALGTGIGDD 484 Query: 482 FDLSKARYHKVVIMTDADVDGAHIRTLLLTFFYRYMRKIIEAGYIYIAQPPLFKIERNKV 541 FD+ KARYHK++IMTDADVDGAHIRTL+LTFFYRYMR +IE GY+YIAQPPL+K+++ K Sbjct: 485 FDIEKARYHKIIIMTDADVDGAHIRTLILTFFYRYMRPLIEHGYVYIAQPPLYKVQQGKD 544 Query: 542 VRYAQTEKERDEIIASFGENVKVNVQRYKGLGEMNAAQLWDTTMDPESRMMLQVTIEDAI 601 ++YA + E EI++ + K +QRYKGLGEMN +QLW+TTMDP R +LQVT+EDA+ Sbjct: 545 IQYAYGDAELQEILSQLPDKAKPGIQRYKGLGEMNPSQLWETTMDPGQRTVLQVTLEDAM 604 Query: 602 LADGIFDTLMGDNVEPRREFIQEHAQSVRNLDI 634 AD IF+ LMGD VEPRR+FIQE+A+ V+NLDI Sbjct: 605 KADEIFEILMGDRVEPRRDFIQENARYVKNLDI 637 >lin0006 [L] COG0187 Type IIA topoisomerase (DNA gyrase/topo II, topoisomerase IV), B subunit Length = 301 Score = 780 bits (2014), Expect = 0.0, Method: Compositional matrix adjust. Identities = 0/631 (0%), Positives = 0/631 (0%), Gaps = 2/631 (0%) Query: 6 YDESQIQVLEGLEAVRKRPGMYIGSTSAKGLHHLVWEVVDNSIDEALAGYCDRIQVKIHE 65 L +K + + + + + + Sbjct: 16 RPNVGKSTLLNKLLGQKISITSRKAQTTRHRIVGIHTEGAYQAIYVDTPGLHMEEKRAIN 75 Query: 66 DNSVTVIDNGRGIPVGENVKLKKSTLEVVMTVLHAXXXXXXXXXXXXXXXXXXXISVVNA 125 + G ++ + ++ + Sbjct: 76 RLMNKAASSSIGDVELVIFVVEGTRWTPDDEMVLNKLREGKAPVILAVNKVDNVQEKADL 135 Query: 126 LSEKVVVNVKRDGHIYQQEYRRGAPQYDIKIVXXXXXXXXXXXFLPDPEIFTETTVFEYT 185 L + + + D P+ I + F + Sbjct: 136 LPHLQFLASQMNFLDIVPISAETGLNVDTIAAIVRKHLPEATHHFPEDYITDRSQRFMAS 195 Query: 186 TLLTRIRELAFLNKGIELSLLDERT--GVSNVFKYEGGIVEYVKYLNEKKEALHEEPIYV 243 ++ + ++ G++ + +K ++ Sbjct: 196 EIIREKLMRFLGAELPYSVTVEIERFVSNERGGYDINGLILVEREGQKKMVIGNKGAKIK 255 Query: 244 EGSRDMIAVEVALQYNDSYTENIYSFANNINTHEGGTHESGFKSALTRIINDYARKTGVI 303 Sbjct: 256 315 Query: 304 KDSSSNLTGDDVREGLTAIISVKIPEPQFEGQTKTKLGNSEVRGVVESLFGEKLQEFLEE 363 Sbjct: 316 375 Query: 364 NPAVSRRVLEKSMQXXXXXXXXXXXXXXXXXXSALEVSALPGKLADCSSKDASISELYIV 423 Sbjct: 376 435 Query: 424 EGDSAGGSAKQGRDRHFQAILPLRGKILNVEKARLDRILSNAEIRAIITALGTGISDDFD 483 Sbjct: 436 495 Query: 484 LSKARYHKVVIMTDADVDGAHIRTLLLTFFYRYMRKIIEAGYIYIAQPPLFKIERNKVVR 543 Sbjct: 496 555 Query: 544 YAQTEKERDEIIASFGENVKVNVQRYKGLGEMNAAQLWDTTMDPESRMMLQVTIEDAILA 603 Sbjct: 556 615 Query: 604 DGIFDTLMGDNVEPRREFIQEHAQSVRNLDI 634 Sbjct: 616 646 >CAC0006 [L] COG0187 Type IIA topoisomerase (DNA gyrase/topo II, topoisomerase IV), B subunit Length = 637 Score = 759 bits (1959), Expect = 0.0, Method: Compositional matrix adjust. Identities = 369/631 (58%), Positives = 473/631 (74%), Gaps = 3/631 (0%) Query: 6 YDESQIQVLEGLEAVRKRPGMYIGSTSAKGLHHLVWEVVDNSIDEALAGYCDRIQVKIHE 65 YDESQIQVLEGLEAVRKRPGMYIGST +GLHHLV+E+VDNSIDEALAGYC I+V IH+ Sbjct: 8 YDESQIQVLEGLEAVRKRPGMYIGSTGTRGLHHLVYEIVDNSIDEALAGYCSHIKVFIHK 67 Query: 66 DNSVTVIDNGRGIPVGENVKLKKSTLEVVMTVLHAXXXXXXXXXXXXXXXXXXXISVVNA 125 DNSVTV D+GRG+P+G + K+KK T+EV+MT+LHA SVVNA Sbjct: 68 DNSVTVSDDGRGMPIGIHHKMKKPTVEVIMTILHAGGKFGGGAYRVSGGLHGVGASVVNA 127 Query: 126 LSEKVVVNVKRDGHIYQQEYRRGAPQYDIKIVXXXXXXXXXXXFLPDPEIFTETTVFEYT 185 LSE V VK +GHI++Q Y RG + + F PDPEIF E T ++Y Sbjct: 128 LSETCEVEVKTEGHIWKQTYHRGKVASPFEKIGDSDEHGTKIYFKPDPEIF-EDTEYDYD 186 Query: 186 TLLTRIRELAFLNKGIELSLLDERTGVSNVFKYEGGIVEYVKYLNEKKEALHEEPIYVEG 245 TL R+RELAFLNKGI++ L DER + +F YEGG+ +V YLN KE + +EPIYVEG Sbjct: 187 TLSQRLRELAFLNKGIKIELTDERHDKNEIFHYEGGLKSFVSYLNRNKEVVFKEPIYVEG 246 Query: 246 SRDM-IAVEVALQYNDSYTENIYSFANNINTHEGGTHESGFKSALTRIINDYARKTGVIK 304 S D +VE+ALQYND Y ENI+SFANNI+T EGGTH +GFK+ALTR+INDYA+K G +K Sbjct: 247 SIDSNYSVEIALQYNDGYNENIFSFANNIHTIEGGTHLAGFKTALTRVINDYAKKFGYLK 306 Query: 305 DSSSNLTGDDVREGLTAIISVKIPEPQFEGQTKTKLGNSEVRGVVESLFGEKLQEFLEEN 364 ++ NL+G+D+REGLTA++SVK+ EPQFEGQTKTKLGN+EVRG+V+S+ E++ +LEEN Sbjct: 307 ENDKNLSGEDIREGLTAVVSVKLTEPQFEGQTKTKLGNTEVRGIVDSIISERVSTYLEEN 366 Query: 365 PAVSRRVLEKSMQXXXXXXXXXXXXXXXXXXSALEVSALPGKLADCSSKDASISELYIVE 424 P + + V++K++ S LE ++LPGKLADCSSKDA E+YIVE Sbjct: 367 PQIGKLVIDKALVASRAREAAKKAREITRRKSVLESTSLPGKLADCSSKDAEECEIYIVE 426 Query: 425 GDSAGGSAKQGRDRHFQAILPLRGKILNVEKARLDRILSNAEIRAIITALGTGISDDFDL 484 GDSAGGSAKQGR+R FQAILPLRGKI+NVEK R+D+IL++ EI+A+ TA G GI DFD+ Sbjct: 427 GDSAGGSAKQGRNRRFQAILPLRGKIMNVEKQRIDKILNSEEIKAMATAFGGGIGKDFDV 486 Query: 485 SKARYHKVVIMTDADVDGAHIRTLLLTFFYRYMRKIIEAGYIYIAQPPLFKIERNKVVRY 544 SK RYHK++IMTDADVDGAHIRTL+LTFFYRYM ++I G+++IAQPPL+K+ + + Y Sbjct: 487 SKLRYHKIIIMTDADVDGAHIRTLILTFFYRYMTELISEGHVFIAQPPLYKVTKTRKEYY 546 Query: 545 AQTEKERDEIIASF-GENVKVNVQRYKGLGEMNAAQLWDTTMDPESRMMLQVTIEDAILA 603 A ++KE ++++ G++ ++QRYKGLGEMN QLW+TTM+PE R +++V IEDA+ A Sbjct: 547 AYSDKELEDVLQDVGGKDKNTDIQRYKGLGEMNPEQLWETTMNPEQRTLIKVNIEDAMAA 606 Query: 604 DGIFDTLMGDNVEPRREFIQEHAQSVRNLDI 634 D IF LMGD V+PRR+FI+E+A V NLD+ Sbjct: 607 DEIFTILMGDKVDPRRKFIEENATKVVNLDV 637 >L0283 [L] COG0187 Type IIA topoisomerase (DNA gyrase/topo II, topoisomerase IV), B subunit Length = 651 Score = 743 bits (1917), Expect = 0.0, Method: Compositional matrix adjust. Identities = 373/631 (59%), Positives = 451/631 (71%), Gaps = 9/631 (1%) Query: 6 YDESQIQVLEGLEAVRKRPGMYIGSTSAKGLHHLVWEVVDNSIDEALAGYCDRIQVKIHE 65 YD SQIQVLEGLEAVR RPGMYIGSTS +GLHHLVWE+VDNSIDEALAG+ I+V I Sbjct: 15 YDASQIQVLEGLEAVRMRPGMYIGSTSKEGLHHLVWEIVDNSIDEALAGFASHIEVFIEP 74 Query: 66 DNSVTVIDNGRGIPVGENVKLKKSTLEVVMTVLHAXXXXXXXXXXXXXXXXXXXISVVNA 125 DNS+TV+D+GRGIPV K + +E V TVLHA SVVNA Sbjct: 75 DNSITVVDDGRGIPVDIQEKTGRPAVETVFTVLHAGGKFGGGGYKVSGGLHGVGSSVVNA 134 Query: 126 LSEKVVVNVKRDGHIYQQEYRRGAPQYDIKIVXXXXXXXXXXXFLPDPEIFTETTVFEYT 185 LS + V V +DG Y QEY RG D+KI+ F+PDPEIFTET F++ Sbjct: 135 LSTVLDVTVHKDGQKYYQEYHRGLVGDDLKIIGETDRRGTTVHFVPDPEIFTETVEFDFD 194 Query: 186 TLLTRIRELAFLNKGIELSLLDERTGVSNV---FKYEGGIVEYVKYLNEKKEALHEEPIY 242 L TR+RELAFLN+G+ +SL D+R G F YEGGI YV+YLNE K + E I+ Sbjct: 195 KLATRVRELAFLNRGLRISLTDKREGNEKNEVHFHYEGGIQSYVEYLNENKTVIFEPAIF 254 Query: 243 VEGSRDMIAVEVALQYNDSYTENIYSFANNINTHEGGTHESGFKSALTRIINDYARKTGV 302 EG D IAVEVA+QY D+Y + SFANNINTHEGGTHE GF++ALTR+IN+YA++ + Sbjct: 255 TEGEMDGIAVEVAMQYTDTYHSVVMSFANNINTHEGGTHEQGFRTALTRVINNYAKQNKI 314 Query: 303 IKDSSSNLTGDDVREGLTAIISVKIPEPQFEGQTKTKLGNSEVRGVVESLFGEKLQEFLE 362 +K++ NLTGDDVREGLTA+ISVK P PQFEGQTKTKLGNSEV G+V LF E L F+ Sbjct: 315 LKENEDNLTGDDVREGLTAVISVKHPNPQFEGQTKTKLGNSEVTGIVNKLFAEALTTFML 374 Query: 363 ENPAVSRRVLEKSMQXXXXXXXXXXXXXXXXXXSALEVSALPGKLADCSSKDASISELYI 422 ENP V+R+++EK + S LE+S LPGKLADCSS + +EL+I Sbjct: 375 ENPQVARKIVEKGILASKARIAAKRAREVTRKKSGLEISNLPGKLADCSSNNPEQTELFI 434 Query: 423 VEGDSAGGSAKQGRDRHFQAILPLRGKILNVEKARLDRILSNAEIRAIITALGTGISDDF 482 VEGDSAGGSAK GR+R FQAILP+RGKILNVEKA +D+IL+N EIR++ TA+GTG D+ Sbjct: 435 VEGDSAGGSAKSGRNREFQAILPIRGKILNVEKATMDKILANEEIRSLFTAMGTGFGADY 494 Query: 483 DLSKARYHKVVIMTDADVDGAHIRTLLLTFFYRYMRKIIEAGYIYIAQPPLFKIE-RNKV 541 DLSKARYHK+VIMTDADVDGAHIRTLLLT FYRYMR ++EAGY+YIAQPP++ I+ ++ Sbjct: 495 DLSKARYHKLVIMTDADVDGAHIRTLLLTLFYRYMRPVVEAGYVYIAQPPIYGIKVGSET 554 Query: 542 VRYAQT----EKERDEIIASFGEN-VKVNVQRYKGLGEMNAAQLWDTTMDPESRMMLQVT 596 Y Q EK+ E +A + + K VQRYKGLGEM+ QLWDTTMDPE R+M +V+ Sbjct: 555 KEYIQPGENQEKDLQEALARWAQGRTKPTVQRYKGLGEMDDHQLWDTTMDPEHRLMARVS 614 Query: 597 IEDAILADGIFDTLMGDNVEPRREFIQEHAQ 627 +EDA AD IFD LMGD VEPRREFI+ +AQ Sbjct: 615 VEDAAEADKIFDMLMGDRVEPRREFIENNAQ 645 Database: COG Posted date: Aug 3, 2010 9:58 AM Number of letters in database: 38,378,431 Number of sequences in database: 115,334 Lambda K H 0.316 0.135 0.373 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 115334 Number of Hits to DB: 41,232,948 Number of extensions: 1585448 Number of successful extensions: 10114 Number of sequences better than 1.0e-05: 101 Number of HSP's gapped: 9471 Number of HSP's successfully gapped: 150 Length of query: 634 Length of database: 38,378,431 Length adjustment: 113 Effective length of query: 521 Effective length of database: 25,345,689 Effective search space: 13205103969 Effective search space used: 13205103969 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.3 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.6 bits) S2: 119 (50.4 bits)