BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0005.0013.p (200 letters) Database: COG 115,334 sequences; 38,378,431 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value mll1981 [IQR] COG1028 Dehydrogenases with different specificitie... 192 2e-49 BS_fabG [IQR] COG1028 Dehydrogenases with different specificitie... 137 1e-32 NMA0533 [IQR] COG1028 Dehydrogenases with different specificitie... 129 2e-30 NMB1921 [IQR] COG1028 Dehydrogenases with different specificitie... 128 6e-30 PM1916 [IQR] COG1028 Dehydrogenases with different specificities... 121 6e-28 >mll1981 [IQR] COG1028 Dehydrogenases with different specificities (related to short-chain alcohol dehydrogenases) Length = 200 Score = 192 bits (489), Expect = 2e-49, Method: Compositional matrix adjust. Identities = 0/183 (0%), Positives = 0/183 (0%) Query: 18 DISKPAQIEQLYKDTKALFGKIDIVVNNAGIMINSLIGEATEEQFDKQFAINVKGTYFSC 77 + + + + + + + + + +F ++ A Sbjct: 76 QLREVDIYQGDTPFCHFAYIEKEGNALMQDLEEEGYLVGLEKAKFVERLAHYYCEINVLH 135 Query: 78 QQAFFHLEQGGRIINFSTSVNGQMFPAYSIYAGTKGAVEQFTRQLAKEFGSKGITINAVA 137 G I Q + Sbjct: 136 PFRVGSGLAQRIFFEQLAIHAGYQLSWQGIEKEAWNQANQSGAMGDLTALQMIFSKVVSE 195 Query: 138 PGPVATDLFLEGKSEAQLEGLKKTNAFGRLGQPEDIAGVVSFLAAEQSGWITGQTLRVNG 197 Sbjct: 196 255 Query: 198 GFI 200 Sbjct: 256 258 >BS_fabG [IQR] COG1028 Dehydrogenases with different specificities (related to short-chain alcohol dehydrogenases) Length = 246 Score = 137 bits (345), Expect = 1e-32, Method: Compositional matrix adjust. Identities = 72/186 (38%), Positives = 113/186 (60%), Gaps = 5/186 (2%) Query: 18 DISKPAQIEQLYKDTKALFGKIDIVVNNAGIMINSLIGEATEEQFDKQFAINVKGTYFSC 77 D+S P ++ + K+T ++F IDI+VNNAGI ++LI E+++D IN+KG F+C Sbjct: 62 DVSNPEDVQNMIKETLSVFSTIDILVNNAGITRDNLIMRMKEDEWDDVININLKG-VFNC 120 Query: 78 QQAF---FHLEQGGRIINFSTSVNGQMFPAYSIYAGTKGAVEQFTRQLAKEFGSKGITIN 134 +A ++ GRIIN S+ V P + Y K V T+ AKE S+ IT+N Sbjct: 121 TKAVTRQMMKQRSGRIINVSSIVGVSGNPGQANYVAAKAGVIGLTKSSAKELASRNITVN 180 Query: 135 AVAPGPVATDLFLEGKSEAQLEGLKKTNAFGRLGQPEDIAGVVSFLAAEQSGWITGQTLR 194 A+APG ++TD+ + + Q E LK+ R G+P D++ VV+FLA+E + ++TGQTL Sbjct: 181 AIAPGFISTDMTDKLAKDVQDEMLKQI-PLARFGEPSDVSSVVTFLASEGARYMTGQTLH 239 Query: 195 VNGGFI 200 ++GG + Sbjct: 240 IDGGMV 245 >NMA0533 [IQR] COG1028 Dehydrogenases with different specificities (related to short-chain alcohol dehydrogenases) Length = 206 Score = 129 bits (325), Expect = 2e-30, Method: Compositional matrix adjust. Identities = 0/186 (0%), Positives = 0/186 (0%), Gaps = 5/186 (2%) Query: 18 DISKPAQIEQLYKDTKALFGKIDIVVNNAGIMINSLIGEATEEQFDKQFAINVKGTYFSC 77 +I+ T + + D + Y + Sbjct: 63 EIALSHAGLAEANVTFVTAKLDTEDGKQEYEIEFYSGNTEYDYDIDASTGEILSYDYDAE 122 Query: 78 QQAFFHL--EQGGRIINFSTSVNGQMFPA-YSIYAGTKGAVEQFTRQLAKEFGSKGITIN 134 + + S + + + + + Sbjct: 123 NYSASGSTQSGDNSTYIG-EEKAKSIALEKVSGATESDIRLYLDYEDGMAVYEGSIVFDS 181 Query: 135 AVAPGPVATDLFLEGKSEAQLEGLKKTNAFGRLGQPEDIAGVVSFLAAEQSGWITGQTLR 194 + Sbjct: 182 MEYEFEIDATT- 240 Query: 195 VNGGFI 200 Sbjct: 241 246 >NMB1921 [IQR] COG1028 Dehydrogenases with different specificities (related to short-chain alcohol dehydrogenases) Length = 248 Score = 128 bits (321), Expect = 6e-30, Method: Compositional matrix adjust. Identities = 74/184 (40%), Positives = 109/184 (59%), Gaps = 5/184 (2%) Query: 20 SKPAQIEQLYKDTKALFGKIDIVVNNAGIMINSLIGEATEEQFDKQFAINVKGTYFSCQQ 79 ++P IE L D + FGK+DI+VNNAGI ++L+ EE++D +N+K + + + Sbjct: 65 AEPETIESLIADIEKAFGKLDILVNNAGITRDNLLMRMKEEEWDDIMQVNLKSVFRASKA 124 Query: 80 AFFHL--EQGGRIINFSTSVNGQMFPA-YSIYAGTKGAVEQFTRQLAKEFGSKGITINAV 136 + ++ GRIIN TSV G M A + YA K + F++ +A+E GS+GIT+N V Sbjct: 125 VLRGMMKQRSGRIINI-TSVVGVMGNAGQTNYAAAKAGLIGFSKSMAREVGSRGITVNCV 183 Query: 137 APGPVATDLFLEGKSEAQLEGLKKTNAFGRLGQPEDIAGVVSFLAAEQSGWITGQTLRVN 196 APG + TD+ E + A GR G +DIA V FLA++Q+ +ITGQTL VN Sbjct: 184 APGFIDTDM-TRALPEETRQTFTAQTALGRFGDAQDIADAVLFLASDQAKYITGQTLHVN 242 Query: 197 GGFI 200 GG + Sbjct: 243 GGML 246 >PM1916 [IQR] COG1028 Dehydrogenases with different specificities (related to short-chain alcohol dehydrogenases) Length = 242 Score = 121 bits (304), Expect = 6e-28, Method: Compositional matrix adjust. Identities = 71/184 (38%), Positives = 105/184 (57%), Gaps = 3/184 (1%) Query: 18 DISKPAQIEQLYKDTKALFGKIDIVVNNAGIMINSLIGEATEEQFDKQFAINVKGTYFSC 77 ++S A IE + KA FG+IDI+VNNAGI ++L+ +E++ N+ Y Sbjct: 57 NVSDLASIETTLEKIKADFGEIDILVNNAGITRDNLLMRMKDEEWFDIMQTNLTSVYHLS 116 Query: 78 QQAFFHL--EQGGRIINFSTSVNGQMFPAYSIYAGTKGAVEQFTRQLAKEFGSKGITINA 135 + + ++ GRII + V P + Y K + F++ LAKE S+GIT+N Sbjct: 117 KAMLRSMMKKRFGRIITIGSVVGSMGNPGQTNYCAAKAGLIGFSKALAKEVASRGITVNV 176 Query: 136 VAPGPVATDLFLEGKSEAQLEGLKKTNAFGRLGQPEDIAGVVSFLAAEQSGWITGQTLRV 195 VAPG +ATD+ + +E Q G+ GRLG P+DIA V+FLA++ +G+ITG TL V Sbjct: 177 VAPGFIATDM-TDVLTEEQKAGILSQVPAGRLGDPKDIAKAVAFLASDDAGYITGTTLHV 235 Query: 196 NGGF 199 NGG Sbjct: 236 NGGM 239 Database: COG Posted date: Aug 3, 2010 9:58 AM Number of letters in database: 38,378,431 Number of sequences in database: 115,334 Lambda K H 0.318 0.136 0.385 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 115334 Number of Hits to DB: 13,230,125 Number of extensions: 560531 Number of successful extensions: 1792 Number of sequences better than 1.0e-05: 288 Number of HSP's gapped: 1377 Number of HSP's successfully gapped: 288 Length of query: 200 Length of database: 38,378,431 Length adjustment: 101 Effective length of query: 99 Effective length of database: 26,729,697 Effective search space: 2646240003 Effective search space used: 2646240003 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.3 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.7 bits) S2: 113 (48.1 bits)