BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0005.0012.p (353 letters) Database: COG 115,334 sequences; 38,378,431 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value lin2727 [T] COG0642 Signal transduction histidine kinase 195 1e-49 BS_phoR_3 [T] COG0642 Signal transduction histidine kinase 149 6e-36 SA1515 [T] COG0642 Signal transduction histidine kinase 147 2e-35 BS_resE_4 [T] COG0642 Signal transduction histidine kinase 137 3e-32 L0122 [T] COG0642 Signal transduction histidine kinase 130 3e-30 >lin2727 [T] COG0642 Signal transduction histidine kinase Length = 182 Score = 195 bits (495), Expect = 1e-49, Method: Compositional matrix adjust. Identities = 0/267 (0%), Positives = 0/267 (0%), Gaps = 1/267 (0%) Query: 84 IPIITAMRQISKGNFKVELENDRRYGQFGSIVEGINEMASELSRMETMRQDFISNVSHEI 143 Sbjct: 192 - 250 Query: 144 QSPLTSIRGFARALRDEGLSAESRAHYLDIIEAEGSRLSGLSDNLLKLSALEAESFPFER 203 Sbjct: 251 310 Query: 204 TAYRLDKQLQEMILACEPQWLDKNIDVEAELEEVTVQAVKDLLSQVWTNLLHNSIKFTPQ 263 Sbjct: 311 370 Query: 264 DGMITVRLRTLDNRVEVEIKDNGIGIAEDDLPRIFERFYKVDKARSTXXXXXXXXXXXVK 323 Sbjct: 371 430 Query: 324 KIVDIHEGSITITSRPGEGTACVVVLP 350 Sbjct: 431 457 >BS_phoR_3 [T] COG0642 Signal transduction histidine kinase Length = 431 Score = 149 bits (376), Expect = 6e-36, Method: Compositional matrix adjust. Identities = 15/230 (6%), Positives = 46/230 (20%), Gaps = 3/230 (1%) Query: 123 SELSRMETMRQDFISNVSHEIQSPLTSIRGFARALRDEGL-SAESRAHYLDIIEAEGSRL 181 + + + +P + L ++ ++ Sbjct: 42 LLIWHFWNLLRLSWWLWVDRSMTPPPGRGSWEPLLYGLHQMQLRNKKRRRELGNLIKRFR 101 Query: 182 SGLSDNLLKLSALEAESFPFERTAYRLDKQLQEMILACEPQWLDKNIDVEAELEEVTVQA 241 SG + E F L+ E T Sbjct: 102 SGAESLPDAVVLTTEEGGIFWCNGLAQQILGLRWPEDNGQNILNLLRYPEFTQYLKTRDF 161 Query: 242 VKD--LLSQVWTNLLHNSIKFTPQDGMITVRLRTLDNRVEVEIKDNGIGIAEDDLPRIFE 299 + L+ +L + +T + ++ R T +++E ++ ++ + + Sbjct: 162 SRPLNLVLNTGRHLEIRVMPYTHKQLLMVARDVTQMHQLEGARRNFFANVSHELRTPLTV 221 Query: 300 RFYKVDKARSTXXXXXXXXXXXVKKIVDIHEGSITITSRPGEGTACVVVL 349 ++ + Sbjct: 222 LQGYLEMMNEQPLEGAVREKALHTMREQTQRMEGLVKQLLTLSKIEAAPT 271 >SA1515 [T] COG0642 Signal transduction histidine kinase Length = 554 Score = 147 bits (372), Expect = 2e-35, Method: Compositional matrix adjust. Identities = 89/230 (38%), Positives = 138/230 (60%), Gaps = 3/230 (1%) Query: 123 SELSRMETMRQDFISNVSHEIQSPLTSIRGFARALRDEGLS-AESRAHYLDIIEAEGSRL 181 + L ++E +R++F++NVSHE+++P+TSI+GFA L D + AES +L+II E +R+ Sbjct: 322 TNLKQLENLRREFVANVSHELKTPITSIKGFAETLIDGAKNDAESLDMFLNIILKESNRI 381 Query: 182 SGLSDNLLKLSALEAESFPFERTAYRLDKQLQEMILACEPQWLDKNIDVEAELE-EVTVQ 240 L +LL LS +E + + L + +I Q KNI + ++E +V V+ Sbjct: 382 ESLVTDLLDLSHIEQHT-ELDTDYMNLSDLTRRIIDNMMTQANQKNISIHTDIEKDVIVK 440 Query: 241 AVKDLLSQVWTNLLHNSIKFTPQDGMITVRLRTLDNRVEVEIKDNGIGIAEDDLPRIFER 300 A + ++QV TNLL N+I ++ +DG I VR+ D RV E++D GIGI +D RIFER Sbjct: 441 AQESKIAQVITNLLTNAINYSYEDGDINVRVYRDDFRVIFEVQDFGIGIKLEDQQRIFER 500 Query: 301 FYKVDKARSTXXXXXXXXXXXVKKIVDIHEGSITITSRPGEGTACVVVLP 350 FY+VDKARS K IV+ H+G+I + S+ G+G+ V+L Sbjct: 501 FYRVDKARSRDSGGTGLGLSITKHIVEAHQGNIEVNSQVGKGSTFKVILK 550 >BS_resE_4 [T] COG0642 Signal transduction histidine kinase Length = 269 Score = 137 bits (345), Expect = 3e-32, Method: Compositional matrix adjust. Identities = 79/233 (33%), Positives = 136/233 (58%), Gaps = 3/233 (1%) Query: 123 SELSRMETMRQDFISNVSHEIQSPLTSIRGFARALRDE-GLSAESRAHYLDIIEAEGSRL 181 +E R++ +R+DFI+NVSHE+++P++ ++G++ A+ D+ S E R II E R+ Sbjct: 36 TEERRLDKLREDFIANVSHELRTPISMLQGYSEAIVDDIASSEEDRKEIAQIIYDESLRM 95 Query: 182 SGLSDNLLKLSALEAESFPFERTAYRLDKQLQEMILACEPQWLDKNI--DVEAELEEVTV 239 L ++LL L+ +E+ +++ L+++I +KNI D + L E Sbjct: 96 GRLVNDLLDLARMESGHTGLHYEKINVNEFLEKIIRKFSGVAKEKNIALDHDISLTEEEF 155 Query: 240 QAVKDLLSQVWTNLLHNSIKFTPQDGMITVRLRTLDNRVEVEIKDNGIGIAEDDLPRIFE 299 +D + QV+TNL+ N+++ T G +++ + ++ + ++++IKD+G GI E+DLP IFE Sbjct: 156 MFDEDKMEQVFTNLIDNALRHTSAGGSVSISVHSVKDGLKIDIKDSGSGIPEEDLPFIFE 215 Query: 300 RFYKVDKARSTXXXXXXXXXXXVKKIVDIHEGSITITSRPGEGTACVVVLPMQ 352 RFYK DKAR+ VK IV+ H GSIT+ SR +GT +P + Sbjct: 216 RFYKADKARTRGRAGTGLGLAIVKNIVEAHNGSITVHSRIDKGTTFSFYIPTK 268 >L0122 [T] COG0642 Signal transduction histidine kinase Length = 517 Score = 130 bits (328), Expect = 3e-30, Method: Compositional matrix adjust. Identities = 74/216 (34%), Positives = 124/216 (57%), Gaps = 6/216 (2%) Query: 135 FISNVSHEIQSPLTSIRGFARALRDEGLSAESRAHYLDIIEAEGSRLSGL-SDNLLKLSA 193 FI+NVSHE+++PLTSI+GFA L + +S+ ++ II E SRL L D LL Sbjct: 307 FIANVSHELKTPLTSIKGFAELLETDSISSSDTKNFASIINKESSRLMNLVQDTLL---- 362 Query: 194 LEAESFPFERTAYRLDKQLQEMILACEPQWLDKNIDVEAELEEVTVQAVKDLLSQVWTNL 253 L ++ ++ L +Q ++ + PQ +K++ E+++EE+T + + ++ ++ NL Sbjct: 363 LTKQNHRIDKKKINLTTLIQNILQSSGPQINEKSLHFESQIEEITFKTNEPMVHSIFENL 422 Query: 254 LHNSIKFTPQDGMITVRLRTLDNRVEVEIKDNGIGIAEDDLPRIFERFYKVDKARSTXXX 313 + N+IK+TP +G I V L N+V + DNG G++E RIF+RFY+VD++R+ Sbjct: 423 IENAIKYTPANGQIFVSLHLEQNKVIFSVTDNGPGLSEIQKERIFDRFYRVDESRAEVSG 482 Query: 314 XXXXXXXXVKKIVDIHEGSITITSRPGEGTACVVVL 349 K + ++H G+I + S G+GT V L Sbjct: 483 TGLGLAIVEKNVQELH-GTIDVVSILGKGTTFTVNL 517 Database: COG Posted date: Aug 3, 2010 9:58 AM Number of letters in database: 38,378,431 Number of sequences in database: 115,334 Lambda K H 0.319 0.136 0.391 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 115334 Number of Hits to DB: 23,034,358 Number of extensions: 759209 Number of successful extensions: 2328 Number of sequences better than 1.0e-05: 212 Number of HSP's gapped: 2067 Number of HSP's successfully gapped: 212 Length of query: 353 Length of database: 38,378,431 Length adjustment: 107 Effective length of query: 246 Effective length of database: 26,037,693 Effective search space: 6405272478 Effective search space used: 6405272478 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.4 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.8 bits) S2: 116 (49.3 bits)