BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0005.0006.p (303 letters) Database: COG 115,334 sequences; 38,378,431 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value BH1245 [G] COG1175 ABC-type sugar transport systems, permease co... 162 4e-40 SMb20326 [G] COG1175 ABC-type sugar transport systems, permease ... 160 2e-39 ML1426 [G] COG1175 ABC-type sugar transport systems, permease co... 155 6e-38 AGc4717 [G] COG1175 ABC-type sugar transport systems, permease c... 153 3e-37 Rv2040c [G] COG1175 ABC-type sugar transport systems, permease c... 150 2e-36 >BH1245 [G] COG1175 ABC-type sugar transport systems, permease components Length = 445 Score = 162 bits (411), Expect = 4e-40, Method: Compositional matrix adjust. Identities = 81/244 (33%), Positives = 140/244 (57%), Gaps = 1/244 (0%) Query: 50 TRLQDAGYIGLENFRHIFTEDDLFYSTLVTTLKWVFSEVALQLVFGLIVALLLNQSFXXX 109 T + ++ +IG ++ + + +D ++ + TL + VA +LV GL +ALL+N++F Sbjct: 189 TAILESNFIGFSHYVN-YVQDSRMWAAMSNTLFFTVVSVAAELVLGLAIALLINRTFKGR 247 Query: 110 XXXXXXXXXPWAVSGVLTTMLWSLMFNQHIGIINDILLKLGIIHEKIAWLANPDTVFGSV 169 PWA+ + M+W +++ G+I +LG+I + L+ S+ Sbjct: 248 GLVRVAVLIPWAIPTAVAAMMWRYLYDGQFGVIAHYFEQLGLISDSSVLLSTGAGAMFSI 307 Query: 170 VFAELWRGIPFFAITLLAALQTIPHEVYESCEVDGAGKLKKLFHITLPYLKESIIFATLL 229 +FA++W+ P+ A+ LLA LQTIP+ +YE+ +VDGA +++ F ITLP LK +I+ A L Sbjct: 308 IFADVWKTTPYMALLLLAGLQTIPNSLYEAAKVDGANRIQTFFKITLPLLKSAILVALLF 367 Query: 230 RAIWEFNSIDMIFTMTNGGPMDMTTTLPIYMMKTSILEGNYGYGSALGVVTFLFLMIFVI 289 R + F D+IF +T GGP + T ++ IY +T + N+G GSAL V+ FL + + Sbjct: 368 RTLDAFRVFDLIFVLTGGGPANSTESISIYAYRTLFSQQNFGAGSALSVIVFLSVALISF 427 Query: 290 LYLK 293 ++K Sbjct: 428 AFIK 431 >SMb20326 [G] COG1175 ABC-type sugar transport systems, permease components Length = 328 Score = 160 bits (406), Expect = 2e-39, Method: Compositional matrix adjust. Identities = 92/314 (29%), Positives = 165/314 (52%), Gaps = 25/314 (7%) Query: 2 EITRQRKRKKGEAWIPYLLIAPTVLLIAGILIFPIFRVFDLSVQSYDFTRLQDAGYIGLE 61 ++ QR R +L +APT L++A + +P+ R S + T L A ++G Sbjct: 22 DLQAQRVRSA------WLFLAPTFLVLALVAGWPLIRTIYFSFTNASLTNLSGAEFVGFA 75 Query: 62 NF---------RHIFT---EDDLFYSTLVTTLKWVFSEVALQLVFGLIVALLLNQSFXXX 109 N+ R I+ D +++ + TLK+ V+++ GLIVAL+LN F Sbjct: 76 NYLSWITLKSGRTIYRGLLADPAWWNAVWNTLKFTVLSVSIETALGLIVALVLNAQFPGR 135 Query: 110 XXXXXXXXXPWAVSGVLTTMLWSLMFNQHIGIINDILLKLGIIHEKIAWLANPDTVFGSV 169 PWA+ +++ +W+ M N GI+ND+L+ LG+I EKIAW A+PDT + Sbjct: 136 GLVRAAILIPWAIPTIVSAKMWAWMLNDQFGILNDMLIGLGLIGEKIAWTASPDTAMIAE 195 Query: 170 VFAELWRGIPFFAITLLAALQTIPHEVYESCEVDGAGKLKKLFHITLPYLKESIIFATLL 229 + ++W+ PF A+ +LA LQ +P ++YE+ ++DG ++ + +TLP ++ +++ A + Sbjct: 196 LIVDVWKTTPFMALLILAGLQMVPGDIYEAAKIDGVHPVRVFWRVTLPLIRPALMVAVIF 255 Query: 230 RAIWEFNSIDMIFTMTNGGPMDMTTTLPIYMMKTSILE-GNYGYGSALGVVTFLFLMIFV 288 R + D+I+ +T P + T M + ++ + + YG+A + FL + Sbjct: 256 RMLDALRIFDLIYVLT---PNNAQTKTMSVMARENLFDFDKFAYGAAASTMLFLIIATIT 312 Query: 289 ILYL---KLNRAGG 299 ILY+ +LN +GG Sbjct: 313 ILYMWLGRLNLSGG 326 >ML1426 [G] COG1175 ABC-type sugar transport systems, permease components Length = 319 Score = 155 bits (393), Expect = 6e-38, Method: Compositional matrix adjust. Identities = 92/289 (31%), Positives = 157/289 (54%), Gaps = 6/289 (2%) Query: 10 KKGEAWIPYLLIAPTVLLIAGILIFPIFRVFDLSVQSYDFTRLQDAGYIGLENFRHIFTE 69 + AW + IAP + ++ ++FP+ +S Q +D ++GL NF+++FT Sbjct: 24 SRRRAWAGRMFIAPNLASVSVFMLFPLGFSLYMSFQKWDM--FTPPVFVGLANFQNLFTS 81 Query: 70 DDLFYSTLVTTLKWVFSEVALQLVFGLIVALLLNQSFXXXXXXXXXXXXPWAVSGVLTTM 129 D LF L ++ + V ++ L+VA +LNQ P A+S V+ + Sbjct: 82 DPLFLIALCNSVVFTVGTVIPTVLISLVVAGVLNQKVKGIGIFRTIVFLPLAISSVVMAV 141 Query: 130 LWSLMFNQHIGIINDILLKLGIIHEKIAWLANPDTVFGSVVFAELWRGIPFFAITLLAAL 189 +W +FN H G++N +L +GI I WL NP S+ +WR +PF + LLAA+ Sbjct: 142 VWQFIFNTHNGLLNIMLGWIGI--GPIPWLVNPGWAMASLCIVSVWRSVPFATVVLLAAM 199 Query: 190 QTIPHEVYESCEVDGAGKLKKLFHITLPYLKESIIFATLLRAIWEFNSIDMIFTMT--NG 247 Q +P VYE+ ++DGAG++++ IT+P+++ +I F ++ I F + D+++ + NG Sbjct: 200 QEVPKTVYEAAKIDGAGEIRQFISITVPFIRGAISFVVVISFIHAFQTFDLVYVLNGPNG 259 Query: 248 GPMDMTTTLPIYMMKTSILEGNYGYGSALGVVTFLFLMIFVILYLKLNR 296 GP T L I + + + +GY SAL +VTF L++ ++L L LNR Sbjct: 260 GPELATYVLGIMLFQHAFSFLEFGYASALALVTFAILLVLIVLQLLLNR 308 >AGc4717 [G] COG1175 ABC-type sugar transport systems, permease components Length = 294 Score = 153 bits (387), Expect = 3e-37, Method: Compositional matrix adjust. Identities = 88/287 (30%), Positives = 148/287 (51%), Gaps = 4/287 (1%) Query: 10 KKGEAWIPYLLIAPTVLLIAGILIFPIFRVFDLSVQSYDFTRLQDAGYIGLENFRHIFTE 69 K+ + L+ P + I I+ +P+ F LS ++ + ++G N+ IF Sbjct: 4 KRSAVIFAWFLLLPALFYITLIVAYPLVDTFILSFTDASLKKVTN--WVGFINYEKIF-- 59 Query: 70 DDLFYSTLVTTLKWVFSEVALQLVFGLIVALLLNQSFXXXXXXXXXXXXPWAVSGVLTTM 129 + F + T W F V+++++ G A+LLN + PW V + Sbjct: 60 NATFADVITRTFVWTFFSVSIKMIIGTFGAVLLNSAVPGRALFRILTMPPWIVPMAIGIF 119 Query: 130 LWSLMFNQHIGIINDILLKLGIIHEKIAWLANPDTVFGSVVFAELWRGIPFFAITLLAAL 189 +W M+N G+I+ +L LG++ +A+LA T F + + ++W G+P + LLAA+ Sbjct: 120 MWGWMYNGQFGMISGVLQNLGLVDGPVAFLAYGRTAFWATIVTDVWIGVPMVTLYLLAAI 179 Query: 190 QTIPHEVYESCEVDGAGKLKKLFHITLPYLKESIIFATLLRAIWEFNSIDMIFTMTNGGP 249 Q+IP ++YE+ DGA + + ITLP + ++I ++L I FNS D+I+ +T GGP Sbjct: 180 QSIPQDLYEAAWTDGASRSYRFRRITLPLMLPAMITMSVLSLISTFNSFDIIWILTRGGP 239 Query: 250 MDMTTTLPIYMMKTSILEGNYGYGSALGVVTFLFLMIFVILYLKLNR 296 TTT+ I KT+I YG G+A V+ +FL IF Y ++ R Sbjct: 240 NGETTTMIIDTYKTAIGAYKYGEGAARAVLICIFLSIFTFFYFRITR 286 >Rv2040c [G] COG1175 ABC-type sugar transport systems, permease components Length = 300 Score = 150 bits (380), Expect = 2e-36, Method: Compositional matrix adjust. Identities = 85/280 (30%), Positives = 146/280 (52%), Gaps = 6/280 (2%) Query: 19 LLIAPTVLLIAGILIFPIFRVFDLSVQSYDFTRLQDAGYIGLENFRHIFTEDDLFYSTLV 78 + +AP + + ++FP+ +S Q +D A ++ L+NFR++FT D LF +V Sbjct: 14 MFVAPNLAAVVVFMLFPLGFSLYMSFQKWDL--FTHATFVRLDNFRNLFTSDPLFLIAVV 71 Query: 79 TTLKWVFSEVALQLVFGLIVALLLNQSFXXXXXXXXXXXXPWAVSGVLTTMLWSLMFNQH 138 T + V ++ L+VA LN+ P A+S V+ ++W +FN Sbjct: 72 NTAVYTVGTVVPTVIVSLVVAAFLNRKIKGISLFRTVVFLPLAISSVVMAVVWQFVFNTD 131 Query: 139 IGIINDILLKLGIIHEKIAWLANPDTVFGSVVFAELWRGIPFFAITLLAALQTIPHEVYE 198 G++N +L LGI I WL P S+ +WR +PF + LLAA+Q +P VYE Sbjct: 132 NGLLNIMLGWLGI--GPIPWLIEPRWAMVSLCLVSVWRSVPFATVVLLAAMQGVPETVYE 189 Query: 199 SCEVDGAGKLKKLFHITLPYLKESIIFATLLRAIWEFNSIDMIFTMT--NGGPMDMTTTL 256 + +DGAG++++ IT+P ++ ++ F ++ I F + D+++ +T NGGP T L Sbjct: 190 AARIDGAGEIRQFVSITVPLIRGALSFVVVISIIHAFQAFDLVYVLTGANGGPETATYVL 249 Query: 257 PIYMMKTSILEGNYGYGSALGVVTFLFLMIFVILYLKLNR 296 I + + + +GY SAL V F L++ +L L++ Sbjct: 250 GIMLFQHAFSFLEFGYASALAWVMFAILLVLTVLQLRITH 289 Database: COG Posted date: Aug 3, 2010 9:58 AM Number of letters in database: 38,378,431 Number of sequences in database: 115,334 Lambda K H 0.329 0.144 0.441 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 115334 Number of Hits to DB: 20,986,382 Number of extensions: 778983 Number of successful extensions: 2543 Number of sequences better than 1.0e-05: 166 Number of HSP's gapped: 2293 Number of HSP's successfully gapped: 166 Length of query: 303 Length of database: 38,378,431 Length adjustment: 106 Effective length of query: 197 Effective length of database: 26,153,027 Effective search space: 5152146319 Effective search space used: 5152146319 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 15 ( 7.1 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 40 (21.8 bits) S2: 115 (48.9 bits)