BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0005.0004.p (370 letters) Database: COG 115,334 sequences; 38,378,431 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value mll0854 [G] COG1653 ABC-type sugar transport system, periplasmic... 94 4e-19 YPO1893 [G] COG1653 ABC-type sugar transport system, periplasmic... 69 1e-11 BMEII0542 [G] COG1653 ABC-type sugar transport system, periplasm... 67 6e-11 lin0762 [G] COG1653 ABC-type sugar transport system, periplasmic... 66 8e-11 PA2338 [G] COG1653 ABC-type sugar transport system, periplasmic ... 63 7e-10 >mll0854 [G] COG1653 ABC-type sugar transport system, periplasmic component Length = 430 Score = 94.0 bits (232), Expect = 4e-19, Method: Compositional matrix adjust. Identities = 81/326 (24%), Positives = 138/326 (42%), Gaps = 10/326 (3%) Query: 43 LEPLDTYFDSWADKDLISSELIASNRKLDTKEGKLYALPYSSQTWNLWLRPDWFKEAGLQ 102 LE L Y W D + ++ + + +PY L+ FK+AGL Sbjct: 106 LEDLGPYMAKWDDAKTLGERAKQFGSTVNNTQ---FMIPYGYYVNALFWNKKLFKQAGLD 162 Query: 103 LPD-TWDQFFEDIAKLTDKSKDRYGLSIRGGAGSANTLEMLMYSYSGITDYFTPEGKSTI 161 P T D+F D K++ +YG +RGG G+ N + M M G YF +G STI Sbjct: 163 GPPATLDEFVADSKKIS-AIPGKYGYCLRGGPGAFNGMHMFMNIAQGKGGYFNKDGTSTI 221 Query: 162 NDPLHAEFLEKYLGAY-NVYTPEDDLNKGWTELAATFQSNKAAIVVHNLGSASSHEKAFG 220 N+ + L+ Y + P+D ++ G+ E F S A++ + + Sbjct: 222 NEEGSVKGLQMLADMYKDGLAPKDAVSWGFNETVTGFYSGTCAMLNQDPDALLGIADKMS 281 Query: 221 GDYTKFEAVPFPKSVKGYREHPGLAPLGLTMSKTAKNKEAVWKFMTFYLSHDINSQYAKL 280 D F P P G + +P L G M +++K+ WK M LS N ++AK Sbjct: 282 AD--DFAVAPLPVGPSG-KSYPTLGYAGWAMFANSQHKDDAWKLMATLLSPKDNLEWAKE 338 Query: 281 YGEIPANKDAASDAWVQDIPYMKSASDLLNSADTHFADNPYYLPGYSN-IQKKAESMIQK 339 G +P + A D + + ++L +++ P +L N + + A Q+ Sbjct: 339 VGVVPIHNGADQDPHFKTEQFKGWFTELSDTSKYEMVTPPTHLENLGNFVDQVAIKNFQE 398 Query: 340 VMAKKMTGTEMLNEWAKLLEAEKQSY 365 V+ + T ++ ++WA L E+Q + Sbjct: 399 VLLGQKTAKDVADQWAAFLTKEQQDW 424 >YPO1893 [G] COG1653 ABC-type sugar transport system, periplasmic component Length = 422 Score = 68.6 bits (166), Expect = 1e-11, Method: Compositional matrix adjust. Identities = 53/198 (26%), Positives = 88/198 (44%), Gaps = 13/198 (6%) Query: 78 YALPYSSQTWNLWLRPDWFKEAGLQLPDTWDQFFEDIAKLTDKSKDRY-GLSIRGGAGSA 136 Y LP ++ L R D F++ G+ +P T+D+ + +K++ + G+++RG G Sbjct: 133 YGLPVYGESTFLMYRKDLFEQYGIAVPKTFDELTAAAKTIKEKTEGKVAGITLRGAQGIQ 192 Query: 137 NTLE--MLMYSYSGITDYFTPEGKSTINDPLHAEFLEKYLGAYNVYTPEDDLNKGWTELA 194 NT ++ Y G + GKS I P E + ++ Y P N GW E Sbjct: 193 NTFAWASFLWGYGG--QWIDDNGKSAITSPQAVEATKSFVNILKNYGPIGAANFGWQENR 250 Query: 195 ATFQSNKAAIVVHNLGSASSHEKAFGGDYT-KFEAVPFPKSVKGYREHPG----LAPLGL 249 FQ KAA+ + + + +E K P P +HPG L GL Sbjct: 251 LVFQQGKAAMTIDSTVNGGFNEDPKESTVVGKVGYAPVPVQPG---DHPGNSGALQVHGL 307 Query: 250 TMSKTAKNKEAVWKFMTF 267 +S +K ++A WKF+++ Sbjct: 308 YISSDSKKQDAAWKFISW 325 >BMEII0542 [G] COG1653 ABC-type sugar transport system, periplasmic component Length = 398 Score = 66.6 bits (161), Expect = 6e-11, Method: Compositional matrix adjust. Identities = 83/352 (23%), Positives = 138/352 (39%), Gaps = 53/352 (15%) Query: 15 QKVNVAISAEQAPD---IMNDFKADLLSRDVLEPLDTYFDSWADKDLISSELIASNRKLD 71 QK+ ISA D I + D + + + EPLD Y + D I + Sbjct: 58 QKLTTDISAGTNADLSIIGTRWLIDFVQQGIAEPLDGYMN-----DEFKGRFIETFLSPS 112 Query: 72 TKEGKLYALPYSSQTWNLWLRPDWFKEAGLQ-LPDTWDQFFEDIAKLTDKSKDRYGLSIR 130 +GK Y LP ++ ++ D F++AG++ P WD+ D AK+ + YG ++ Sbjct: 113 VLDGKTYGLPIAASVRAMYYNKDLFEKAGIKNPPANWDELKADAAKIKALGGENYGFGLQ 172 Query: 131 GGAGSANTLEMLMYSYSGI----TDYFTPEGKSTINDP-------LHAEFLEKYLGAYNV 179 G +E +Y Y + T+ +G S ++ P L+ ++ L V Sbjct: 173 G-----KEIETDVYYYYAMWSYGTEILNKDGTSGLSTPGALEAAKLYKSMIDDGLTQPGV 227 Query: 180 --YTPEDDLN-----KGWTELAATFQSNKAAIVVHNLGSASSHEKAFGGDYTKFEAVPFP 232 Y ED N K + A F SN+ NL K+ P Sbjct: 228 TSYAREDVQNLFKQGKVGMMITAPFLSNQIKEEAPNL---------------KYGVAAIP 272 Query: 233 KSVKGYREHPGLAPLGLTMSKTAKNKEAVWKFMTFYLSHDINSQYAKLYGEIPANKDAAS 292 G R G+ + M + +KNKE WK + F D +++ + G +P NK+ A Sbjct: 273 AGPTGARGTYGVTD-SVIMFQNSKNKEEAWKVLDFLFQKDWRAKFTQNEGFLPVNKEEAK 331 Query: 293 DAWVQDIPYMKSASDLLNSADTHFADNPYYLPGYSNIQKKAESMIQKVMAKK 344 + + + + + LL D FA +PG+ I + +Q + K Sbjct: 332 MDYYVNNADLAAFTALL--PDARFAP---VIPGWEEIADITSNAMQSIYLGK 378 >lin0762 [G] COG1653 ABC-type sugar transport system, periplasmic component Length = 417 Score = 66.2 bits (160), Expect = 8e-11, Method: Compositional matrix adjust. Identities = 79/298 (26%), Positives = 131/298 (43%), Gaps = 30/298 (10%) Query: 14 EQKVNVAISAEQAPDIM--NDFKADLLS-RDVLEPLDTYFDSWADKDLISSELIASNRKL 70 E+K+ I+ + PDI+ + ++ L + + VLEPLD + ++ + + K Sbjct: 75 EEKLLTGIAGGELPDIIMWDRYQTALYAPKGVLEPLDKLV---KEDNVKMDDFYEESVKE 131 Query: 71 DTKEGKLYALPYSSQTWNLWLRPDWFKEAGLQLPDTWDQFFEDIAKLT--DKSK-DRYGL 127 T KLY LP + L+ +EAG++ P TWD+ K T D +K + G+ Sbjct: 132 MTYSDKLYGLPLLNDNRILFYNKKLLQEAGVKPPTTWDELATAAQKTTKWDGNKMTQAGM 191 Query: 128 SIRGGAGSANTLEMLMYSYSGITDYFTPEGKST-INDPLHAEFL---EKYLGAYNVYTPE 183 S++ G N +Y + TP+ K T N E L +K VY Sbjct: 192 SLQ-DVGLFN-----LYLMQAGGELVTPDNKKTAFNSEQGLEVLNYWDKMQNDLKVY--- 242 Query: 184 DDLNKGWTELAATFQSNKAAIVVHNLGSASSHEKAFGGDYTKFEAVPFPKSVKGYREHPG 243 +G+ + + F + K A+ + + + + K DY E P KG Sbjct: 243 ---QRGFDDGSDAFAAGKEAMTYNGPWALADYNKVENLDYGVVEPPKGPNGDKGAI---- 295 Query: 244 LAPLGLTMSKTAKNKEAVWKFMTFYLSHDINS-QYAKLYGEIPANKDAASDAWVQDIP 300 + GL M K A++K+ W FM ++ + N ++AK+ G +PANK AA D + P Sbjct: 296 MGGFGLVMPKQAEHKDGAWDFMKWWTTKPENGVEFAKISGWLPANKIAAEDEYFTKDP 353 >PA2338 [G] COG1653 ABC-type sugar transport system, periplasmic component Length = 436 Score = 63.2 bits (152), Expect = 7e-10, Method: Compositional matrix adjust. Identities = 75/318 (23%), Positives = 130/318 (40%), Gaps = 40/318 (12%) Query: 62 ELIASNRKLDTKEGKLYALPYSSQTWNLWLRPDWFKEAGLQLPD--TWDQFFEDIAKLTD 119 +L+ S R + +G LYALP+ ++ + R D F++AGL++P+ TW Q E A+L Sbjct: 113 DLLPSVRDGLSVKGTLYALPFYAEASITYYRKDLFQQAGLRMPEQPTWTQLGEFAARLNR 172 Query: 120 KSKDRYGLSIRGGAGSANTLEMLMYSYSGITDYFTPEGKSTINDPLHAEF--------LE 171 + +YG+ +RG AG + ++ + + F G ++ EF L+ Sbjct: 173 PDQGQYGICLRGKAGWGENMALI----GTLANAF---GARWFDERWQPEFSGGEWKKALD 225 Query: 172 KYLGAYNVYTPEDDLNKGWTELAATFQSNKAAI-----VVHNLGSASSHEKAFGGDYTKF 226 Y+ Y P + G+ E A F S K AI V + + S K D T F Sbjct: 226 FYVSTLKRYGPPGASSNGFNENLALFNSGKCAIWVDASVAGSFVTDKSQSKV--ADATGF 283 Query: 227 EAVPFPKSVKGYREHPGLAPLGLTMSKTAKNKEAVWKFMTFYLSHDINSQYAKLYG--EI 284 P + KG L L + +++ K+A F T+ S A G + Sbjct: 284 AFAPREVTDKG---ASWLYSWALAIPASSRAKDAAKAFATWATSQAYGKLVADREGVANV 340 Query: 285 PANKDAA--SDAWVQDIPYMKSASDLLNSADTH---FADNPYY------LPGYSNIQKKA 333 P A+ S+A++ P+ + + L D + PY +P + I + Sbjct: 341 PPGTRASTYSEAYLAAAPFARVTLESLKRVDPNHPTLKPVPYVGIQLVTIPEFQAIGTQV 400 Query: 334 ESMIQKVMAKKMTGTEML 351 + + +M+ + L Sbjct: 401 GKLFSAALTGQMSSDQAL 418 Database: COG Posted date: Aug 3, 2010 9:58 AM Number of letters in database: 38,378,431 Number of sequences in database: 115,334 Lambda K H 0.313 0.130 0.387 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 115334 Number of Hits to DB: 28,863,331 Number of extensions: 1221107 Number of successful extensions: 3299 Number of sequences better than 1.0e-05: 29 Number of HSP's gapped: 3303 Number of HSP's successfully gapped: 29 Length of query: 370 Length of database: 38,378,431 Length adjustment: 108 Effective length of query: 262 Effective length of database: 25,922,359 Effective search space: 6791658058 Effective search space used: 6791658058 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.2 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 42 (21.9 bits) S2: 116 (49.3 bits)