BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0005.0002.p (526 letters) Database: COG 115,334 sequences; 38,378,431 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value CAP0114 [G] COG3507 Beta-xylosidase 216 1e-55 CC2802 [G] COG3507 Beta-xylosidase 145 1e-34 BH3683 [G] COG3507 Beta-xylosidase 131 2e-30 CAC3452 [G] COG3507 Beta-xylosidase 129 1e-29 CC0989 [G] COG3507 Beta-xylosidase 125 2e-28 >CAP0114 [G] COG3507 Beta-xylosidase Length = 531 Score = 216 bits (549), Expect = 1e-55, Method: Compositional matrix adjust. Identities = 171/541 (31%), Positives = 256/541 (47%), Gaps = 74/541 (13%) Query: 16 WIPDQGNGTYINPIIYADYSDPDVIRVGEDFFMTASSFTHIPGLPILHSRDLVNWRIVNH 75 + D GNGTY NP++YADY D VI+VG ++M SS ++PGLPIL S+DLVNW I ++ Sbjct: 33 YTSDLGNGTYQNPVMYADYPDNSVIKVGSTYYMACSSINYMPGLPILKSQDLVNWEICSY 92 Query: 76 ALPRLE--LPGYDKVQ------------HGKGVWAPSLRYHEGQFWIFYATPDEG-IFMT 120 A R++ + G DK + KG WAPSL+YH G F+ +A+ D G F+ Sbjct: 93 AFDRIDTGMSGIDKTHADAYNLVGGENVYSKGCWAPSLKYHNGTFYATFASLDLGKTFVC 152 Query: 121 KTDDP--FGAWEPPHLLKAAKGWI-DPCPFWDEDGQAYLVHAYAHSRSGLKHILNLCRMS 177 P FG W + + G+ D F+D DG+ YL++ N+ ++ Sbjct: 153 TKTGPMGFGGWTFTAI--SGIGYAHDADLFFDTDGRVYLINGGC----------NVTELT 200 Query: 178 PDGTTLLDDGT--IIVDGTVKHPTLEGPKMYKRNGYYYIFA---PAGGVPTGWQAVFRSK 232 PD ++ G I DG P+ +G +++K+NGYYYI + G + +RSK Sbjct: 201 PDCKSVKYGGINQKIFDG---GPSHDGNRIFKKNGYYYILSTPIKGNGAYQRIEKAWRSK 257 Query: 233 SIYGPYEDKIVLQQGETPVNGPHQGGWVELASGESWFVHFQDKGAYGRITHLQPMEWRGD 292 S+ GPYE I+L G HQ V+ S +W + F+DKGA GRI L P+ W + Sbjct: 258 SLTGPYEQTIILDDGAN-----HQVCVVDDGS-YNWAMLFEDKGAVGRIPKLAPVTWVNN 311 Query: 293 WPVMGRLAPDREAGEPVERWKKPDTAAGEVCIPQTSDDFCGAALGL-QWQWQANP-ASNW 350 WP++G + A P + KP G + P T DDF L QWQW NP S W Sbjct: 312 WPMIG---VNGSATVP-NIYNKPAAGNG-IKSPGTDDDFTSQGYVLSQWQWNHNPDNSKW 366 Query: 351 HRLLPGGGLRLNSAPLPGDCRTLYDTPQLLMQKLAAPVFTATVKITPRFSSCAERAGLVV 410 G +RL ++ + L Q++ P + VK+ + +GL Sbjct: 367 TTSERPGWMRLKTSY----SKDFLQARNTLTQRIQGPASSGWVKLDTTNIKPGQISGLSA 422 Query: 411 LGHRAFCIALSLDPAGDLWLAQYESGAADDPSRKDLGVKAAGILIQAESLYLRASFE-EA 469 + I + D G L QY ++ I + +YL+ S + A Sbjct: 423 FHSKYGYIGVRNDN-GTKKLVQYNVTGSE-----------TAINLTNNIVYLKVSTDCNA 470 Query: 470 NGCRFSFSREGSDYIEFGSPFRLE---EGQWVGAKVGLFALSLTPGEDL-GYADFERFTI 525 +F +S G+ + + GS +++ + +VG + LF + T G D GYADF+ F Sbjct: 471 QTAKFYYSYNGNSWTQLGSTLKMDFFYKLWFVGYRFALF--NYTTGSDTSGYADFDYFKF 528 Query: 526 S 526 S Sbjct: 529 S 529 >CC2802 [G] COG3507 Beta-xylosidase Length = 548 Score = 145 bits (367), Expect = 1e-34, Method: Compositional matrix adjust. Identities = 135/488 (27%), Positives = 215/488 (44%), Gaps = 43/488 (8%) Query: 21 GNGTYINPIIYADYSDPDVIRVGEDFFMTASSFTHIPGLPILHSRDLVNWRIVNHALPRL 80 G G Y NP++ Y DP V R G+DF++ S+F PGLP+ HS DLV+W + +A+ R Sbjct: 31 GPGQYRNPVVQGFYPDPSVTRAGKDFYLVNSTFAWFPGLPVFHSTDLVHWEQIGNAIDRP 90 Query: 81 ELPGYDKVQHGKGVWAPSLRYHEGQFWIFYATPD-EGIFMTKTDDPFGAWEPPHLLKAAK 139 + + ++ + V+AP+++ H+G F+I D G ++ DP G W P L Sbjct: 91 GMLDFKRLGLSRAVFAPAIQAHQGMFYILNTCVDCGGNYLITAKDPKGPWSDPVWLPKVG 150 Query: 140 GWIDPCPFWDED---GQAYLVH---AYAHSRSGLKHILNLCRMSPDGTTLLDDGTIIVDG 193 G IDP F+D++ G+ ++V+ L + P T + T+++D Sbjct: 151 G-IDPSIFFDDEDGGGRVWIVNNDGPPGPPEYPGHRALWIHEYDPKTQTTVGPATLLLDK 209 Query: 194 TVKHPTL----EGPKMYKRNGYYYIFAPAGGVPTGW-QAVFRSKSIYG---PYEDKIVLQ 245 V T EGP + K++G+YY+ A GG G Q V RSKS+ G PY + +L Sbjct: 210 GVDPATKPIWPEGPHILKKDGWYYLTAAEGGTAEGHSQVVLRSKSVTGPYVPYTNNPILT 269 Query: 246 QGETPVNGP------HQGGWVELASGESWFVHF------QDKGAYGRITHLQPMEWRGDW 293 Q + P + P V+ G+ W D GR T L P+EW+ W Sbjct: 270 QRDLPRDRPLPITSAGHADIVDTPDGKWWATFLAVRPYGDDLYNTGRETFLLPVEWKDGW 329 Query: 294 PVMGRLAPDREAGEPVERWKKPDTAAGEVCIP-QTSDDFCGAALGLQWQWQANPASNWHR 352 PV+ L + + K P P + ++ F G L + W P+ W Sbjct: 330 PVI--LENGKTIPYVADAPKLPALKTAPTSGPFKAAETFEGPELPMSWMTMRIPSKRWWS 387 Query: 353 LLPGGGLRLNSAPLPGDCRTLYDTPQLLMQKLAAPVFTATVKITPRFSSCA--ERAGLVV 410 + G L L + D ++ P + ++ A+V T RF A ++AGLV Sbjct: 388 -VKDGTLSLEAR---KDRVGGHEQPSIWARRQQH--MNASVSTTVRFDPKADGDKAGLVA 441 Query: 411 LGHRAFCIALSLDPAGDLWLAQYES-GAADDPSRKDLGVKAAGILIQAESLYLRASFEEA 469 + + F + L AG L + E ++ DP + + L +SL L+ + A Sbjct: 442 VQNDDFYWFVGLVQAGGKTLVRVEKRTSSKDPLDGAVVAEKPVSLKSGQSLDLKIA---A 498 Query: 470 NGCRFSFS 477 G F+ Sbjct: 499 RGGALDFA 506 >BH3683 [G] COG3507 Beta-xylosidase Length = 528 Score = 131 bits (330), Expect = 2e-30, Method: Compositional matrix adjust. Identities = 122/409 (29%), Positives = 173/409 (42%), Gaps = 36/409 (8%) Query: 27 NPIIYADYSDPDVIRVGEDFFMTASSFTHIPGLPILHSRDLVNWRIVNHALPRLELPGYD 86 NPI+ + DP ++RVG+D+++ S+F PG+ I HSRDL +WR V+ L R Sbjct: 7 NPILPGFHPDPSIVRVGDDYYIATSTFEWFPGVRIHHSRDLKHWRFVSSPLTRTSQLDMK 66 Query: 87 KVQHGKGVWAPSLRYHEGQFWIFY--------ATPDEGIFMTKTDDPFGAWEPPHLLKAA 138 + G+WAP L YH+G F++ Y A D ++ + G W P L ++ Sbjct: 67 GNMNSGGIWAPCLSYHDGTFYLIYTDVKQWHGAFKDAHNYLVTAQNIEGPWSDPIYLNSS 126 Query: 139 KGWIDPCPFWDEDGQAYLVHAYAHSRSGLKHI--LNLCRMSPDGTTLLDDGTIIVDGTVK 196 DP F D+DG+ +LV+ R G + L S L+ I GT Sbjct: 127 G--FDPSLFHDDDGRKWLVNMIWDYRKGNHPFAGIILQEYSEAEQKLVGPVKNIYKGTDI 184 Query: 197 HPTLEGPKMYKRNGYYYIFAPAGGVPTGWQAVF-RSKSIYGPYEDK----IVLQQG--ET 249 T EGP +YK++GYYY+ GG A RS+SI GPYE +V G E Sbjct: 185 QLT-EGPHLYKKDGYYYLLVAEGGTEYEHAATLARSQSIDGPYETDPSYPLVTSTGQPEL 243 Query: 250 PVNGPHQGGWVELASGESWFVHFQD---KGAY---GRITHLQPMEWRGDWPVMGRLAPDR 303 + G VE +GE + H KG Y GR T +Q + W D G L + Sbjct: 244 ALQKAGHGSLVETQNGEWYLAHLCGRPLKGKYCTLGRETAIQKVNWTED----GWLRIED 299 Query: 304 EAGEPVERWKKPDTAAGEVCIPQTSDDFCGAALGLQWQWQANPAS-NWHRLLPG-GGLRL 361 P+ PD DDF L QW PA +W L G LRL Sbjct: 300 GGNHPLREVTAPDLPEHPFEKEPELDDFDAPQLHHQWNTLRIPADPSWCSLEERPGHLRL 359 Query: 362 NSAPLPGDCRTLYDTPQLLMQKLAAPVFTATVKITPRFSSCAERAGLVV 410 + T + L+ ++ + K+ + S AGLV+ Sbjct: 360 RGM----ESLTSVHSQSLVARRQQSFHCEVETKLEYQPESFQHMAGLVI 404 >CAC3452 [G] COG3507 Beta-xylosidase Length = 533 Score = 129 bits (324), Expect = 1e-29, Method: Compositional matrix adjust. Identities = 139/546 (25%), Positives = 219/546 (40%), Gaps = 70/546 (12%) Query: 27 NPIIYADYSDPDVIRVGEDFFMTASSFTHIPGLPILHSRDLVNWRIVNHALPRLELPGYD 86 NPI+ DP + R D+++ S+F PG+ I HS+DLVNW +V H L R L Sbjct: 4 NPILRGFNPDPSICRADTDYYIATSTFEWFPGVQIHHSKDLVNWHLVAHPLNRTSLLDMK 63 Query: 87 KVQHGKGVWAPSLRYHEGQFWIFYAT--------PDEGIFMTKTDDPFGAWEPPHLLKAA 138 + G+WAP L YH+G+FW+ Y D ++T + G W P L + Sbjct: 64 GNPNSGGIWAPDLSYHDGKFWLIYTDVKVTDGMWKDCHNYLTTCESVDGVWSDPITLNGS 123 Query: 139 KGWIDPCPFWDEDGQAYLVHAYAHSRSGLKHI--LNLCRMSPDGTTLLDDGTIIVDGTVK 196 D F D DG+ YLV+ Y R+ + + L S L+ II GT Sbjct: 124 G--FDASLFHDNDGKKYLVNMYWDQRTYNHNFYGIVLQEYSDKEKKLIGKAKIIYKGTDI 181 Query: 197 HPTLEGPKMYKRNGYYYIFAPAGGVP-TGWQAVFRSKSIYGPYE---DKIVLQQGETPVN 252 T EGP +Y YYY+F GG + V RSK+I GPYE + +L P N Sbjct: 182 KYT-EGPHIYHIGDYYYLFTAEGGTTYEHSETVARSKNIDGPYEIDPEYPLLTSWHDPRN 240 Query: 253 GPHQGGWVELA---SGESWFVH-------------FQDKGA--YGRITHLQPMEWRGDWP 294 + G L + E + H + +G GR T +Q +EW +WP Sbjct: 241 SLQKCGHASLVHTHTDEWYLAHLVGRPLPVGNQPVLEQRGYCPLGRETSIQRIEWVDNWP 300 Query: 295 VMGRLAPDREAGEPVERWKKPDTAAGEVCIPQTSDDFCGAALGLQWQWQANPASNWHRLL 354 R+ ++ VE K P+ + D+F L + +Q P + L Sbjct: 301 ---RVVGGKQGSVNVEAPKIPEVKWEKTY--DEKDNFDSDKLNINFQSLRIPLTENIASL 355 Query: 355 PG--GGLRLNSAPLPGDCRTLYDTPQLLMQKLAAPVFTATVKITPRFSSCAERAGLVVLG 412 G LRL + T T + ++ + F A+ ++ + + AGL Sbjct: 356 KAKKGNLRLYGK----ESLTSTFTQAFIARRWQSFKFDASTSVSFSPDTFQQAAGLTCYY 411 Query: 413 H----RAFCIALSLDPAGDLWLAQYESGAADDPSR-------KDLGVKAAGILIQAESLY 461 + + + D + + ++ D P + KD+ + ++ E+ Sbjct: 412 NTENWSTIQVTWNEDKGRVIDIVCCDNFHFDMPLKSNVIPIPKDVEYIHLKVEVRVETYQ 471 Query: 462 LRASFEEANGCR----FSFSREGSDYIEFGSPFRLEEGQWVGAKVGLFALSLTPGEDLGY 517 SF+ N + F + DY++ G G + GA VG+ + +T Sbjct: 472 YSYSFDGINWSKVPAIFESRKLSDDYVQGG-------GFFTGAFVGINCIDITGNNKP-- 522 Query: 518 ADFERF 523 ADF+ F Sbjct: 523 ADFDYF 528 >CC0989 [G] COG3507 Beta-xylosidase Length = 546 Score = 125 bits (313), Expect = 2e-28, Method: Compositional matrix adjust. Identities = 119/426 (27%), Positives = 180/426 (42%), Gaps = 60/426 (14%) Query: 27 NPIIYADYSDPDVIRVGEDFFMTASSFTHIPGLPILHSRDLVNWRIVNHALPRLELPGYD 86 NP++ DP ++RVGED+++ S+F PG+ I HSRDL NWR++ L R Sbjct: 8 NPVLRGFNPDPSIVRVGEDYYIATSTFEWFPGVQIHHSRDLRNWRLIGRPLRRPSQLNMM 67 Query: 87 KVQHGKGVWAPSLRYHEGQFWIFY-----------------ATPDEGIFMTKTDDPFGAW 129 G GVWAP L + +G+FW+ + + D ++ + G W Sbjct: 68 GDPDGCGVWAPCLTHADGRFWLIFTDVKRYGRTTVGGASGASLRDFHNYLVTCETIDGDW 127 Query: 130 EPPHLLKAAKGWIDPCPFWDEDGQAYLVHAYAHSRSGLKHI--LNLCRMSPDGTTLLDDG 187 P L ++ DP F D+DG+ +LV+ R G + L S L+ + Sbjct: 128 SDPIHLNSSG--FDPSLFHDDDGRKWLVNQLWDHRPGRNRFAGIVLQAFSVAEQRLVGER 185 Query: 188 TIIVDGTVKHPTLEGPKMYKRNGYYYIFAPAGGVPTGW---QAVFRSKSIYGPYE---DK 241 +I +GT T E P +YKR+G+YY+ GG TGW + RS++I GPYE D Sbjct: 186 KVIFEGTAIGLT-EAPHLYKRDGWYYLLTAEGG--TGWGHAATLARSRAIDGPYELHPDT 242 Query: 242 IVL---QQGETPVNGPHQGGWVELASGESWFVHF-------QDKGAYGRITHLQPMEWRG 291 +L + P+ G E A GE++ + + + GR T +Q + W Sbjct: 243 YLLTARDRPHAPLQRAGHGDLFETADGETYIAYLCGRPLPGRGRCVLGRETAIQKLTWGA 302 Query: 292 D-WPVMGRLAPDREAGEPVERWKKPDTAAGEVCIP----QTSDDFCGAALGLQWQWQANP 346 D WP + D P AG V P +DF G L + +QW P Sbjct: 303 DGWPRTLDGSGDPTLETP---------GAGLVDQPWPTTPVREDFDGTTLPIDFQWLRTP 353 Query: 347 ASNWHRLLPG--GGLRLNSAPLPGDCRTLYDTPQLLMQKLAAPVFTATVKITPRFSSCAE 404 L G LRL G T L+ ++ A ++A ++ + + Sbjct: 354 YPETIFSLTARPGFLRLYGRESLGSVF----TQALVARRQQAHCYSAATRLDFEPAHFQQ 409 Query: 405 RAGLVV 410 AGLV Sbjct: 410 AAGLVC 415 Database: COG Posted date: Aug 3, 2010 9:58 AM Number of letters in database: 38,378,431 Number of sequences in database: 115,334 Lambda K H 0.320 0.139 0.458 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 115334 Number of Hits to DB: 47,154,142 Number of extensions: 2193070 Number of successful extensions: 3160 Number of sequences better than 1.0e-05: 8 Number of HSP's gapped: 3131 Number of HSP's successfully gapped: 8 Length of query: 526 Length of database: 38,378,431 Length adjustment: 111 Effective length of query: 415 Effective length of database: 25,576,357 Effective search space: 10614188155 Effective search space used: 10614188155 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.4 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.8 bits) S2: 118 (50.1 bits)