BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0004.0002.p (90 letters) Database: COG 115,334 sequences; 38,378,431 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value BS_hbs [L] COG0776 Bacterial nucleoid DNA-binding protein 112 2e-25 BH1309 [L] COG0776 Bacterial nucleoid DNA-binding protein 110 6e-25 BH3356 [L] COG0776 Bacterial nucleoid DNA-binding protein 107 3e-24 L102317 [L] COG0776 Bacterial nucleoid DNA-binding protein 106 1e-23 BU032 [L] COG0776 Bacterial nucleoid DNA-binding protein 96 2e-20 >BS_hbs [L] COG0776 Bacterial nucleoid DNA-binding protein Length = 92 Score = 112 bits (279), Expect = 2e-25, Method: Compositional matrix adjust. Identities = 54/89 (60%), Positives = 64/89 (71%) Query: 1 MNKTDLVNNISEKSGLAKKDVETVLNGVLGEITDALAQGDKVQLIGFGTFETRKRSGRSG 60 MNKT+L+N ++E S L+KKD ++ V I DAL GDK+QLIGFG FE R+RS R G Sbjct: 1 MNKTELINAVAEASELSKKDATKAVDSVFDTILDALKNGDKIQLIGFGNFEVRERSARKG 60 Query: 61 RNPQTGTPIEIPESTVPAFKAGNKLKEAV 89 RNPQTG IEIP S VPAFK G LK+AV Sbjct: 61 RNPQTGEEIEIPASKVPAFKPGKALKDAV 89 >BH1309 [L] COG0776 Bacterial nucleoid DNA-binding protein Length = 90 Score = 110 bits (275), Expect = 6e-25, Method: Compositional matrix adjust. Identities = 52/90 (57%), Positives = 67/90 (74%) Query: 1 MNKTDLVNNISEKSGLAKKDVETVLNGVLGEITDALAQGDKVQLIGFGTFETRKRSGRSG 60 MNKTDL+N ++E++ L+KKD ++ V IT AL +G KVQL+GFG+FE R+RS R G Sbjct: 1 MNKTDLINAVAEQAELSKKDASKAVDAVFESITGALKEGGKVQLVGFGSFEVRERSARKG 60 Query: 61 RNPQTGTPIEIPESTVPAFKAGNKLKEAVN 90 RNPQTG IEIP + PAFK G +LK+AVN Sbjct: 61 RNPQTGEEIEIPATKNPAFKPGKQLKDAVN 90 >BH3356 [L] COG0776 Bacterial nucleoid DNA-binding protein Length = 90 Score = 107 bits (268), Expect = 3e-24, Method: Compositional matrix adjust. Identities = 49/89 (55%), Positives = 67/89 (75%) Query: 1 MNKTDLVNNISEKSGLAKKDVETVLNGVLGEITDALAQGDKVQLIGFGTFETRKRSGRSG 60 MNKT+L++ ++E++ ++KKD V+N V I ++LAQGD VQLIGFG FE R+R+ R G Sbjct: 1 MNKTELIHQVAERTQMSKKDAGEVVNTVFDVIAESLAQGDSVQLIGFGNFEVRERAARKG 60 Query: 61 RNPQTGTPIEIPESTVPAFKAGNKLKEAV 89 RNPQTG I+I + PAFKAG +LK+AV Sbjct: 61 RNPQTGEEIDIAATKTPAFKAGKQLKDAV 89 >L102317 [L] COG0776 Bacterial nucleoid DNA-binding protein Length = 91 Score = 106 bits (264), Expect = 1e-23, Method: Compositional matrix adjust. Identities = 51/88 (57%), Positives = 65/88 (73%) Query: 2 NKTDLVNNISEKSGLAKKDVETVLNGVLGEITDALAQGDKVQLIGFGTFETRKRSGRSGR 61 NK DL+ ++ K+GL KKD E +N +T+ LA+G+KVQLIGFGTFETR+R+ R GR Sbjct: 3 NKQDLIAEVAAKTGLTKKDSEKAVNAFGEVVTEFLAKGEKVQLIGFGTFETRERAAREGR 62 Query: 62 NPQTGTPIEIPESTVPAFKAGNKLKEAV 89 NPQTG I+I + VPAFKAG LK+AV Sbjct: 63 NPQTGEAIKIAATVVPAFKAGKALKDAV 90 >BU032 [L] COG0776 Bacterial nucleoid DNA-binding protein Length = 92 Score = 95.5 bits (236), Expect = 2e-20, Method: Compositional matrix adjust. Identities = 45/89 (50%), Positives = 61/89 (68%) Query: 1 MNKTDLVNNISEKSGLAKKDVETVLNGVLGEITDALAQGDKVQLIGFGTFETRKRSGRSG 60 MNKT L+N IS+KS L+K + L L I D+L +G+ VQ++GFGTF+ RS R+G Sbjct: 1 MNKTQLINVISKKSNLSKIQAKLTLETTLSTIIDSLKKGESVQIVGFGTFKVNLRSSRTG 60 Query: 61 RNPQTGTPIEIPESTVPAFKAGNKLKEAV 89 RNPQTG I+IP + VP+F +G LK A+ Sbjct: 61 RNPQTGKEIQIPATKVPSFTSGKTLKNAI 89 Database: COG Posted date: Aug 3, 2010 9:58 AM Number of letters in database: 38,378,431 Number of sequences in database: 115,334 Lambda K H 0.308 0.130 0.351 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 115334 Number of Hits to DB: 7,189,939 Number of extensions: 287264 Number of successful extensions: 1480 Number of sequences better than 1.0e-05: 86 Number of HSP's gapped: 1480 Number of HSP's successfully gapped: 86 Length of query: 90 Length of database: 38,378,431 Length adjustment: 60 Effective length of query: 30 Effective length of database: 31,458,391 Effective search space: 943751730 Effective search space used: 943751730 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.1 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 42 (21.6 bits) S2: 109 (46.6 bits)