BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0003.0003.p (309 letters) Database: COG 115,334 sequences; 38,378,431 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value BH1928 [K] COG1609 Transcriptional regulators 231 1e-60 STM1430 [K] COG1609 Transcriptional regulators 108 1e-23 BS_purR [F] COG0503 Adenine/guanine phosphoribosyltransferases a... 104 2e-22 ECs2367 [K] COG1609 Transcriptional regulators 104 2e-22 BS_degA [K] COG1609 Transcriptional regulators 99 1e-20 >BH1928 [K] COG1609 Transcriptional regulators Length = 335 Score = 231 bits (588), Expect = 1e-60, Method: Compositional matrix adjust. Identities = 116/235 (49%), Positives = 154/235 (65%) Query: 1 LTTIYDIAKKTGYSPTTVSKAFNNYSDVREKTRQEILRTAQEMGYLPNAHARTLTTKKSW 60 + TIYDIAKKTGYS TTVSK NNY+DV +K R++++ EMGY P++ AR LTTKKSW Sbjct: 1 MATIYDIAKKTGYSITTVSKVLNNYTDVSDKARKKVMDAVTEMGYFPSSSARMLTTKKSW 60 Query: 61 TIGVLFVEGTGAGIRHPFFSAVIESFKQVAVAKGYALMFISKDVGGKQSGYLENCRIRGV 120 TIGV+FVE G G+ HPFFS+VIE+FK+ GY L+F S +G + YLE+ R RGV Sbjct: 61 TIGVVFVESAGIGMEHPFFSSVIENFKKNVERFGYDLLFASNQIGNEAKTYLEHFRYRGV 120 Query: 121 DGVVVFLSDYNDPYFLELLESDIPTVILDYNTPQSHTVCSDNRAGALLAVNYLASLXXXX 180 DG+VV S NDP +L+++DIP+V++D ++ S V SDN G+ LAV+YL SL Sbjct: 121 DGIVVVCSLLNDPEVEKLMKADIPSVVIDLDSKGSSAVYSDNEYGSELAVDYLVSLGHRS 180 Query: 181 XXXXXXXXXXFPGSERELGYIAAMEELGLDVPDGYIAEGAFFSRESGYDAMLKLL 235 F G +R G+ A+++L L + D YI +G FF+ E G AM LL Sbjct: 181 IAHISGDQGLFVGVQRLKGFKDAIQKLNLSISDEYIVDGGFFTYEGGQRAMEALL 235 >STM1430 [K] COG1609 Transcriptional regulators Length = 341 Score = 108 bits (269), Expect = 1e-23, Method: Compositional matrix adjust. Identities = 78/241 (32%), Positives = 121/241 (50%), Gaps = 13/241 (5%) Query: 1 LTTIYDIAKKTGYSPTTVSKAFNNYSDVREKTRQEILRTAQEMGYLPNAHARTLTTKKSW 60 + TI D+AK+ S TTVS N V E+TR + +E+ Y P+A AR+L + Sbjct: 1 MATIKDVAKRANVSTTTVSHVINKTRFVAEETRNAVWAAIKELHYSPSAVARSLKVNHTK 60 Query: 61 TIGVLFVEGTGAGIRHPFFSAVIESFKQVAVAKGYALMFISK-DVGGKQSGYLENCRIRG 119 +IG+L A +F+ +IE+ ++ KGY L+ + + KQ YL + Sbjct: 61 SIGLLATSSEAA-----YFAEIIEAVEKNCFQKGYTLILGNAWNNLEKQRAYLSMMAQKR 115 Query: 120 VDGVVVFLSDYNDPYFLELLES--DIPTVILDYNTPQ---SHTVCSDNRAGALLAVNYLA 174 VDG++V S+Y +P L +LE IP V++D+ + + TV + AG +A YL Sbjct: 116 VDGLLVMCSEYPEP-LLSMLEEYRHIPMVVMDWGEAKADFTDTVIDNAFAGGYMAGRYLV 174 Query: 175 SLXXXXXXXXXXXXXXFPGSERELGYIAAMEELGLDVPDGYIAEGAFFSRESGYDAMLKL 234 G+ R G++ AMEE ++VPD +I +G F ESGY AM ++ Sbjct: 175 ERGHRDIGVIPGPLERNTGAGRLAGFMKAMEEALINVPDNWIVQGD-FEPESGYHAMQQI 233 Query: 235 L 235 L Sbjct: 234 L 234 >BS_purR [F] COG0503 Adenine/guanine phosphoribosyltransferases and related PRPP-binding proteins Length = 301 Score = 104 bits (260), Expect = 2e-22, Method: Compositional matrix adjust. Identities = 14/250 (5%), Positives = 46/250 (18%), Gaps = 13/250 (5%) Query: 1 LTTIYDIAKKTGYSPTTVSKAFNNYSDVREKTRQEILRTAQEMGYLPNAHARTLTTKKSW 60 ++ + + + R AQ + + + Sbjct: 1 MSIDKSYCGFIAIVGRPNVGKSTLLNKLLGQKISITSRKAQTTRHRIVGIHTEGAYQAIY 60 Query: 61 TIGVLFVEGTGAGIRHPFFSAVIESFKQVAVAKGYALMFISK-DVGGKQSGYLENCRIRG 119 + ++ ++ ++F+ + + N G Sbjct: 61 VDTPGLHMEEKR-----AINRLMNKAASSSIGDVELVIFVVEGTRWTPDDEMVLNKLREG 115 Query: 120 VDGVVVFLSDYNDPYFLELLES--DIPTVILDYNTPQ---SHTVCSDNRAGALLAVNYLA 174 V++ ++ ++ + S + A + +L Sbjct: 116 KAPVILAVNKVDNV-QEKADLLPHLQFLASQMNFLDIVPISAETGLNVDTIAAIVRKHLP 174 Query: 175 SLXXXXXXXXXXXXXXFPGSERELGYIAAMEELGLDVPDGYIAEGAFFSRESGYDAMLKL 234 + + + F + Sbjct: 175 EATHHFPEDYITDRSQRFMASEIIREKLMRFLGAELPYSVTVEIER-FVSNERGGYDING 233 Query: 235 LELPECRNAV 244 L L E Sbjct: 234 LILVEREGQK 243 >ECs2367 [K] COG1609 Transcriptional regulators Length = 341 Score = 104 bits (260), Expect = 2e-22, Method: Compositional matrix adjust. Identities = 78/250 (31%), Positives = 121/250 (48%), Gaps = 13/250 (5%) Query: 1 LTTIYDIAKKTGYSPTTVSKAFNNYSDVREKTRQEILRTAQEMGYLPNAHARTLTTKKSW 60 + TI D+AK+ S TTVS N V E+TR + +E+ Y P+A AR+L + Sbjct: 1 MATIKDVAKRANVSTTTVSHVINKTRFVAEETRNAVWAAIKELHYSPSAVARSLKVNHTK 60 Query: 61 TIGVLFVEGTGAGIRHPFFSAVIESFKQVAVAKGYALMFISK-DVGGKQSGYLENCRIRG 119 +IG+L A +F+ +IE+ ++ KGY L+ + + KQ YL + Sbjct: 61 SIGLLATSSEAA-----YFAEIIEAVEKNCFQKGYTLILGNAWNNLEKQRAYLSMMAQKR 115 Query: 120 VDGVVVFLSDYNDPYFLELLES--DIPTVILDYNTPQ---SHTVCSDNRAGALLAVNYLA 174 VDG++V S+Y +P L +LE IP V++D+ + + V + G +A YL Sbjct: 116 VDGLLVMCSEYPEP-LLAMLEEYRHIPMVVMDWGEAKADFTDAVIDNAFEGGYMAGRYLI 174 Query: 175 SLXXXXXXXXXXXXXXFPGSERELGYIAAMEELGLDVPDGYIAEGAFFSRESGYDAMLKL 234 G+ R G++ AMEE + VP+ +I +G F ESGY AM ++ Sbjct: 175 ERGHREIGVIPGPLERNTGAGRLAGFMKAMEEAMIKVPESWIVQGD-FEPESGYRAMQQI 233 Query: 235 LELPECRNAV 244 L P AV Sbjct: 234 LSQPHRPTAV 243 >BS_degA [K] COG1609 Transcriptional regulators Length = 337 Score = 98.6 bits (244), Expect = 1e-20, Method: Compositional matrix adjust. Identities = 75/248 (30%), Positives = 118/248 (47%), Gaps = 16/248 (6%) Query: 2 TTIYDIAKKTGYSPTTVSKAFNNYSDVREKTRQEILRTAQEMGYLPNAHARTLTTKKSWT 61 TTIYD+AK G S TTVS+ NN + +KTRQ+++ EM Y PN HA LT K++ Sbjct: 3 TTIYDVAKAAGVSITTVSRVINNTGRISDKTRQKVMNVMNEMAYTPNVHAAALTGKRTNM 62 Query: 62 IGVLFVEGTGAGIRHPFFSAVIESFKQVAVAKGYALMFISKDVG-GKQSGYLENCRIRGV 120 I ++ I +PF+ + +S ++ A G+ ++ S D K++ Y + + V Sbjct: 63 IALV-----APDISNPFYGELAKSIEERADELGFQMLICSTDYDPKKETKYFSVLKQKKV 117 Query: 121 DGVV--VFLSDYNDPYFLELLESD-IPTVILDYNTP--QSHTVCSDNRAGALLAVNYLAS 175 DG++ + ++ LE + S+ IP ++ + P V D+ G A +L S Sbjct: 118 DGIIFATGIESHDSMSALEEIASEQIPIAMISQDKPLLPMDIVVIDDVRGGYEAAKHLLS 177 Query: 176 LXXXXXXXXXXXXXXFPGSERELGYIAAMEELGLDVPDGYIAEGAFFSRESGYDAMLKLL 235 L R G+ AMEE G+ + + I + FS ESG + KLL Sbjct: 178 LGHTNIACIIGDGSTTGEKNRIKGFRQAMEEAGVPIDESLIIQ-TRFSLESGKEEAGKLL 236 Query: 236 ELPECRNA 243 + RNA Sbjct: 237 D----RNA 240 Database: COG Posted date: Aug 3, 2010 9:58 AM Number of letters in database: 38,378,431 Number of sequences in database: 115,334 Lambda K H 0.319 0.135 0.410 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 115334 Number of Hits to DB: 23,180,159 Number of extensions: 890486 Number of successful extensions: 1737 Number of sequences better than 1.0e-05: 128 Number of HSP's gapped: 1663 Number of HSP's successfully gapped: 128 Length of query: 309 Length of database: 38,378,431 Length adjustment: 106 Effective length of query: 203 Effective length of database: 26,153,027 Effective search space: 5309064481 Effective search space used: 5309064481 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.4 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.8 bits) S2: 116 (49.3 bits)