BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0001.0011.p (407 letters) Database: COG 115,334 sequences; 38,378,431 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value aq_1229 [GEPR] COG0477 Permeases of the major facilitator superf... 89 9e-18 aq_1220 [M] COG1807 4-amino-4-deoxy-L-arabinose transferase and ... 89 9e-18 aq_1224 [J] COG0482 Predicted tRNA(5-methylaminomethyl-2-thiouri... 89 9e-18 TM1090 [J] COG0124 Histidyl-tRNA synthetase 76 8e-14 YPR033c [J] COG0124 Histidyl-tRNA synthetase 67 5e-11 >aq_1229 [GEPR] COG0477 Permeases of the major facilitator superfamily Length = 403 Score = 89.4 bits (220), Expect = 9e-18, Method: Compositional matrix adjust. Identities = 87/345 (25%), Positives = 148/345 (42%), Gaps = 41/345 (11%) Query: 14 GVRDYLPRAVTKLRKIEKDVLHCMSRWGYRQMITPTLEYYD----TVGVASSTSDQKLYK 69 G D L + K RKI + + + ++I P +EY + +VG + ++++ Sbjct: 8 GFHDILGKDAKKFRKISDTARKILKLYNFEEIILPVVEYAELFQRSVGETTDIVQKEMFV 67 Query: 70 LLNNRGQALVLRSEMTAPVAR--VVSSLLKDEPLPLRLSYHANVXXXXXXXXXXXXXXXQ 127 + +G+ L LR E TA R + L P ++L Y + Q Sbjct: 68 FEDRKGRKLALRPEGTAGTVRAFIQHKLYALRPY-VKLFYEGPMFRYERPQAGRYRQFHQ 126 Query: 128 TGVELVGDDSPEADAEVVALAISSLQAAGVKSFKIAMGHVGFLDG--LFQEAVAG-LPDA 184 G E+ G P ADAE++ + LQA G+K + + +G ++EA+ L Sbjct: 127 IGAEVFGVAEPHADAEIIKIVYDILQALGIKGVVVEINSLGCKKDREAYREALLNYLTGV 186 Query: 185 QEELKSHLLNRDYVAFRETLRRLELSEAQKNELDGLLRLRGGKEICGQALELSSHPLARA 244 +EEL S ++R R LR L+ E C A+ A Sbjct: 187 KEELCSDCISR---MDRNPLRVLDCK----------------VETCKVAVRE-----APK 222 Query: 245 SIEHLC----KVWEVLVSYGVSQHVLI--DLTMIGDFSYYTGMTFEGYASELGFPVCSGG 298 I+ LC + +E L +Y + + + ++ YYT FE + ELG + +GG Sbjct: 223 MIDFLCDECREHYEKLKNYLKALDIPFRENYNLVRGLDYYTRTVFEAVSDELGLTLIAGG 282 Query: 299 RYDNLLQQF-GRPVPSTGFSLKTNRILDGVSGVPEEEELPILIQY 342 RYD L+++ G P P+ GF+L R++ + E+EE+ +I + Sbjct: 283 RYDYLVEELGGPPTPALGFALGVERLMLLLPDEEEKEEVYFVIPF 327 >aq_1220 [M] COG1807 4-amino-4-deoxy-L-arabinose transferase and related glycosyltransferases of PMT family Length = 403 Score = 89.4 bits (220), Expect = 9e-18, Method: Compositional matrix adjust. Identities = 87/345 (25%), Positives = 148/345 (42%), Gaps = 41/345 (11%) Query: 14 GVRDYLPRAVTKLRKIEKDVLHCMSRWGYRQMITPTLEYYD----TVGVASSTSDQKLYK 69 G D L + K RKI + + + ++I P +EY + +VG + ++++ Sbjct: 8 GFHDILGKDAKKFRKISDTARKILKLYNFEEIILPVVEYAELFQRSVGETTDIVQKEMFV 67 Query: 70 LLNNRGQALVLRSEMTAPVAR--VVSSLLKDEPLPLRLSYHANVXXXXXXXXXXXXXXXQ 127 + +G+ L LR E TA R + L P ++L Y + Q Sbjct: 68 FEDRKGRKLALRPEGTAGTVRAFIQHKLYALRPY-VKLFYEGPMFRYERPQAGRYRQFHQ 126 Query: 128 TGVELVGDDSPEADAEVVALAISSLQAAGVKSFKIAMGHVGFLDG--LFQEAVAG-LPDA 184 G E+ G P ADAE++ + LQA G+K + + +G ++EA+ L Sbjct: 127 IGAEVFGVAEPHADAEIIKIVYDILQALGIKGVVVEINSLGCKKDREAYREALLNYLTGV 186 Query: 185 QEELKSHLLNRDYVAFRETLRRLELSEAQKNELDGLLRLRGGKEICGQALELSSHPLARA 244 +EEL S ++R R LR L+ E C A+ A Sbjct: 187 KEELCSDCISR---MDRNPLRVLDCK----------------VETCKVAVRE-----APK 222 Query: 245 SIEHLC----KVWEVLVSYGVSQHVLI--DLTMIGDFSYYTGMTFEGYASELGFPVCSGG 298 I+ LC + +E L +Y + + + ++ YYT FE + ELG + +GG Sbjct: 223 MIDFLCDECREHYEKLKNYLKALDIPFRENYNLVRGLDYYTRTVFEAVSDELGLTLIAGG 282 Query: 299 RYDNLLQQF-GRPVPSTGFSLKTNRILDGVSGVPEEEELPILIQY 342 RYD L+++ G P P+ GF+L R++ + E+EE+ +I + Sbjct: 283 RYDYLVEELGGPPTPALGFALGVERLMLLLPDEEEKEEVYFVIPF 327 >aq_1224 [J] COG0482 Predicted tRNA(5-methylaminomethyl-2-thiouridylate) methyltransferase, contains the PP-loop ATPase domain Length = 403 Score = 89.4 bits (220), Expect = 9e-18, Method: Compositional matrix adjust. Identities = 87/345 (25%), Positives = 148/345 (42%), Gaps = 41/345 (11%) Query: 14 GVRDYLPRAVTKLRKIEKDVLHCMSRWGYRQMITPTLEYYD----TVGVASSTSDQKLYK 69 G D L + K RKI + + + ++I P +EY + +VG + ++++ Sbjct: 8 GFHDILGKDAKKFRKISDTARKILKLYNFEEIILPVVEYAELFQRSVGETTDIVQKEMFV 67 Query: 70 LLNNRGQALVLRSEMTAPVAR--VVSSLLKDEPLPLRLSYHANVXXXXXXXXXXXXXXXQ 127 + +G+ L LR E TA R + L P ++L Y + Q Sbjct: 68 FEDRKGRKLALRPEGTAGTVRAFIQHKLYALRPY-VKLFYEGPMFRYERPQAGRYRQFHQ 126 Query: 128 TGVELVGDDSPEADAEVVALAISSLQAAGVKSFKIAMGHVGFLDG--LFQEAVAG-LPDA 184 G E+ G P ADAE++ + LQA G+K + + +G ++EA+ L Sbjct: 127 IGAEVFGVAEPHADAEIIKIVYDILQALGIKGVVVEINSLGCKKDREAYREALLNYLTGV 186 Query: 185 QEELKSHLLNRDYVAFRETLRRLELSEAQKNELDGLLRLRGGKEICGQALELSSHPLARA 244 +EEL S ++R R LR L+ E C A+ A Sbjct: 187 KEELCSDCISR---MDRNPLRVLDCK----------------VETCKVAVRE-----APK 222 Query: 245 SIEHLC----KVWEVLVSYGVSQHVLI--DLTMIGDFSYYTGMTFEGYASELGFPVCSGG 298 I+ LC + +E L +Y + + + ++ YYT FE + ELG + +GG Sbjct: 223 MIDFLCDECREHYEKLKNYLKALDIPFRENYNLVRGLDYYTRTVFEAVSDELGLTLIAGG 282 Query: 299 RYDNLLQQF-GRPVPSTGFSLKTNRILDGVSGVPEEEELPILIQY 342 RYD L+++ G P P+ GF+L R++ + E+EE+ +I + Sbjct: 283 RYDYLVEELGGPPTPALGFALGVERLMLLLPDEEEKEEVYFVIPF 327 >TM1090 [J] COG0124 Histidyl-tRNA synthetase Length = 346 Score = 76.3 bits (186), Expect = 8e-14, Method: Compositional matrix adjust. Identities = 18/332 (5%), Positives = 50/332 (15%), Gaps = 41/332 (12%) Query: 9 FEKPAGVRDYLPRAVTKLRKIEKDVLHCMSRWGYRQMITPTLE----YYDTVGVASSTSD 64 + + + + + + + + Sbjct: 3 YHRIPHSSLEVSTLGLGTMTFGEQNSEADAHAQLDYAVAQGINLIDVAEMYPVPPRPETQ 62 Query: 65 QKLYKLLNNRGQALVLRSEMTAPVARV-VSSLLKDEPLPLRLSYHANVXXXXXXXXXXXX 123 + N R ++ S P + N+ Sbjct: 63 GLTETYVGNWLAKHGSREKLIIASKVSGPSRNNDKGIRPDQALDRKNIREALHDSLKRLQ 122 Query: 124 XXXQTGVELVGDDSPEADAEVVALAISSLQAAGVKSFKIAMGHVGFLDGLFQEAVAGLPD 183 ++ P + + + A + AG Sbjct: 123 TDYLDLYQVHWPQRPTNCFGKLGYSWTDSAPAVSLLDTLDALAEY--------QRAGKIR 174 Query: 184 AQEELKSHLLNRDYVAFRETLRRLELSEAQKNELDGLLRLRGGKEICGQALELSSHPLAR 243 L + + + Sbjct: 175 YIGVSNET-----AFGVMRYL----HLADKHDLPRIVTIQNP----YSLLNRSFEV--GL 219 Query: 244 ASIEHLC--------KVWEVLVSYGVSQHVLIDLTMIGDFSYYTGMTFEGYASELGF--P 293 A + + ++ + + + G ++ Sbjct: 220 AEVSQYEGVELLAYSCLGFGTLTGKYL--NGAKPAGARNTLFSRFTRYSGEQTQKAVAAY 277 Query: 294 VCSGGRYDNLLQQF-GRPVPSTGFSLKTNRIL 324 V R+ Q V F T Sbjct: 278 VDIARRHGLDPAQMALAFVRRQPFVASTLLGA 309 >YPR033c [J] COG0124 Histidyl-tRNA synthetase Length = 346 Score = 67.0 bits (162), Expect = 5e-11, Method: Compositional matrix adjust. Identities = 0/356 (0%), Positives = 0/356 (0%), Gaps = 48/356 (13%) Query: 12 PAGVRDYLPRAVTKLRKIEKDVLHCMSRWGYRQMITPTLEYYDTVGVASSTSDQKLYKLL 71 + V + + + ++ Sbjct: 58 RPETQGLTETYVGNWLAKHGSREKLIIASKVSGPSRNNDKGIRPDQALDRKNIREALHDS 117 Query: 72 NNRGQALVLRSEMTAPVARVVSSLLKDEPLPLRLSYHANVXXXXXXXXXXXXXXX--QTG 129 R Q L R + L + A + Sbjct: 118 LKRLQTDYLDLYQVHWPQRPTNC---FGKLGYSWTDSAPAVSLLDTLDALAEYQRAGKIR 174 Query: 130 VELVGD-DSPEADAEVVALAISSLQAAGVKSFKIAMGHVGFLDGLFQEAVAGLPDAQ-EE 187 V + + + L ++ + F GL + V+ + Sbjct: 175 YIGVSNETAFGVMRYLHLADKHDLPRIVTIQNPYSLLNRSFEVGLAE--VSQYEGVELLA 232 Query: 188 LKSHLLNRDYVAFRETLRRLELSEAQKNE----LDGLLRLRGG-KEI---CGQALELSSH 239 + + + + Sbjct: 233 YSCLGFGTLTGKYLNGAKPAGARNTLFSRFTRYSGEQTQKAVAAYVDIARRHGLDPAQMA 292 Query: 240 PLARASIEHLCKVWEVLVSYGVSQHVLIDLTMIGDFSYYTGMTFEGYASELGFP------ 293 + + + + L + + P Sbjct: 293 LAFVRRQPFVASTLLGATTMDQLKTNIESLHLELSEDVLAEIEAVHQVYTYPAP 352 Query: 294 ------------------VCSGGRYDNLLQQFGRP-------VPSTGFSLKTNRIL 324 Sbjct: 353 408 Database: COG Posted date: Aug 3, 2010 9:58 AM Number of letters in database: 38,378,431 Number of sequences in database: 115,334 Lambda K H 0.317 0.136 0.389 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 115334 Number of Hits to DB: 27,425,331 Number of extensions: 1010779 Number of successful extensions: 1723 Number of sequences better than 1.0e-05: 5 Number of HSP's gapped: 1711 Number of HSP's successfully gapped: 5 Length of query: 407 Length of database: 38,378,431 Length adjustment: 109 Effective length of query: 298 Effective length of database: 25,807,025 Effective search space: 7690493450 Effective search space used: 7690493450 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.3 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.7 bits) S2: 117 (49.7 bits)