BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0001.0010.p (296 letters) Database: COG 115,334 sequences; 38,378,431 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value AGl997 [S] COG1917 Uncharacterized conserved protein, contains d... 246 3e-65 BS_gntZ [G] COG0362 6-phosphogluconate dehydrogenase 172 7e-43 STM0438 [R] COG0790 FOG: TPR repeat, SEL1 subfamily 163 3e-40 YGR256w [G] COG0362 6-phosphogluconate dehydrogenase 157 2e-38 YHR183w [G] COG0362 6-phosphogluconate dehydrogenase 150 3e-36 >AGl997 [S] COG1917 Uncharacterized conserved protein, contains double-stranded beta-helix domain Length = 82 Score = 246 bits (628), Expect = 3e-65, Method: Compositional matrix adjust. Identities = 0/326 (0%), Positives = 0/326 (0%), Gaps = 38/326 (11%) Query: 1 MIGLGKMGYNLALNLLNHGHDLVVSDINPQRGLQLAERGALPASSLEDMVTKLEHPRILW 60 ++ + + + VV + ++ V L Sbjct: 5 LLYRWRRLLSEGGAAAVDSDEPVVGNSEVKKLEDRVRELERMLGRKTMEVEILREALSKA 64 Query: 61 VMVPAGEIVTGVIDTLAGLLEAGDIIIDGGNSHYKESIVRSGKLRERGIYLFDAGTSGGT 120 Sbjct: 65 DSKKRI- 123 Query: 121 EGAEHGACFMVGGDAEVFTVIEPLFRDLA---------------VERGYLYSGESGSGHF 165 Sbjct: 124 183 Query: 166 LKMVHNGIEYGMMQSIAEGFELLEKSS----------------------FDYNYENVAQL 203 Sbjct: 184 243 Query: 204 WSNGSVIRGWLMELAQNAFAKDPKLSGIRGVMHSSGEGKWTVQTALDLETSTPVIALSLL 263 Sbjct: 244 303 Query: 264 MRYRSLEDDTFHGKVVAALRNEFGGH 289 Sbjct: 304 329 >BS_gntZ [G] COG0362 6-phosphogluconate dehydrogenase Length = 468 Score = 172 bits (435), Expect = 7e-43, Method: Compositional matrix adjust. Identities = 108/285 (37%), Positives = 157/285 (55%), Gaps = 19/285 (6%) Query: 1 MIGLGKMGYNLALNLLNHGHDLVV----SDINPQRGLQLAERGALPASSLEDMVTKLEHP 56 +IGLG MG N+ALN+ N G ++ V D+ Q +L + P LED V LE P Sbjct: 7 VIGLGVMGSNIALNMANKGENVAVYNYTRDLTDQLIQKLDGQSLSPYYELEDFVQSLEKP 66 Query: 57 RILWVMVPAGEIVTGVIDTLAGLLEAGDIIIDGGNSHYKESIVRSGKLRERGIYLFDAGT 116 R +++MV AG+ V VI +L LLE GD+I+DGGNSHY+++ R +L+E+GI G Sbjct: 67 RKIFLMVTAGKPVDSVIQSLKPLLEEGDVIMDGGNSHYEDTERRYDELKEKGIGYLGVGI 126 Query: 117 SGGTEGAEHGACFMVGGDAEVFTVIEPLFRDLAVERG----YLYSGESGSGHFLKMVHNG 172 SGG GA G M GGD +V+ P+ +A + G +Y G G+GHF KMVHNG Sbjct: 127 SGGEVGALTGPSIMPGGDRDVYEKAAPILTKIAAQVGDDPCCVYIGPKGAGHFTKMVHNG 186 Query: 173 IEYGMMQSIAEGFELLEKSSFDYNYENVAQL---WSNGSVIRGWLMELAQNAFAKDPKLS 229 IEY MQ IAE + L + + + +A + W+ G ++ +L+E+ K + + Sbjct: 187 IEYADMQLIAEAYTFL-RETLRLPLDEIASIFETWNQGE-LKSYLIEITAEILRKKDEKT 244 Query: 230 G---IRGVMHSSGE---GKWTVQTALDLETSTPVIALSLLMRYRS 268 G I ++ +G+ GKWT A+D + +I SL RY S Sbjct: 245 GQPLIDVILDKTGQKGTGKWTSMQAIDNGIPSTIITESLFARYLS 289 >STM0438 [R] COG0790 FOG: TPR repeat, SEL1 subfamily Length = 673 Score = 163 bits (413), Expect = 3e-40, Method: Compositional matrix adjust. Identities = 23/282 (8%), Positives = 49/282 (17%), Gaps = 18/282 (6%) Query: 1 MIGLGKMGYNLALNLLNHGHDLVVSDINPQRGLQLAERGAL-----PASSLEDMVTKLEH 55 + L L G N L + + E Sbjct: 7 QQQAVEFVTGPCLVLAGAGSGKTRVITNKIAHLIRGCGYQARHIAAVTFTNKAAREMKER 66 Query: 56 PRILWVMVPAGEIVTGVIDTLAGLLEAGDIIIDGGNSHYKESIVRSGKLRERGIYLFDAG 115 A ++ TL + + G +++ + + Sbjct: 67 VGQTLGRKEARGLMISTFHTLGLDIIKREYAALGMKANFSLFDDTDQLALLKELTEGLIE 126 Query: 116 TSGGTEGAEHGACFMVGGDAEVFTVIEPLFRDLAVER----GYLYSGESGSGHFLKMVHN 171 D + + LY + + L Sbjct: 127 DDKVLLQQLISTISNWKNDLKTPSQAAASAIGERDRIFAHCYGLYDAHLKACNVLDFDDL 186 Query: 172 GIEYGMMQSIAEGFELLEK--SSFDYNYENVAQLWSNGSVIRGWLMELAQNAFAK----- 224 + ++ E + + E S + L+ Sbjct: 187 ILLPTLLLQANEEVRKRWQNKIRYLLVDEYQDTNTSQYE-LVKLLVGSRARFTVVGDDDQ 245 Query: 225 -DPKLSGIRGVMHSSGEGKWTVQTALDLETSTPVIALSLLMR 265 G R + + LE + L Sbjct: 246 SIYSWRGARPQNLVLLSQDFPALKVIKLEQNYRSSGRILKAA 287 >YGR256w [G] COG0362 6-phosphogluconate dehydrogenase Length = 492 Score = 157 bits (398), Expect = 2e-38, Method: Compositional matrix adjust. Identities = 100/288 (34%), Positives = 153/288 (53%), Gaps = 17/288 (5%) Query: 1 MIGLGKMGYNLALNLLNHGHDLVV-----SDINPQRGLQLAERGALPASSLEDMVTKLEH 55 ++GL MG NL LN +HG +V S ++ + + + A+S+ED+V KL+ Sbjct: 10 LVGLAVMGQNLILNAADHGFTVVAYNRTQSKVDRFLANEAKGKSIIGATSIEDLVAKLKK 69 Query: 56 PRILWVMVPAGEIVTGVIDTLAGLLEAGDIIIDGGNSHYKESIVRSGKLRERGIYLFDAG 115 PR + +++ AG V +I L L+ GDIIIDGGNSH+ ++ R +L ++GI +G Sbjct: 70 PRKIMLLIKAGAPVDTLIKELVPHLDKGDIIIDGGNSHFPDTNRRYEELTKQGILFVGSG 129 Query: 116 TSGGTEGAEHGACFMVGGDAEVFTVIEPLFRDLAV----ERGYLYSGESGSGHFLKMVHN 171 SGG +GA G M GG AE + I+ +F+ +A E + G +GSGH++KMVHN Sbjct: 130 VSGGEDGARFGPSLMPGGSAEAWPHIKNIFQSIAAKSNGEPCCEWVGPAGSGHYVKMVHN 189 Query: 172 GIEYGMMQSIAEGFELLEK--SSFDYNYENVAQLWSNGSVIRGWLMELAQNAFAKD---- 225 GIEYG MQ I E ++++++ D V W+ G V+ +L+E+ ++ D Sbjct: 190 GIEYGDMQLICEAYDIMKRIGRFTDKEISEVFDKWNTG-VLDSFLIEITRDILKFDDVDG 248 Query: 226 -PKLSGIRGVMHSSGEGKWTVQTALDLETSTPVIALSLLMRYRSLEDD 272 P + I G GKWT ALDL +I ++ R S D Sbjct: 249 KPLVEKIMDTAGQKGTGKWTAINALDLGMPVTLIGEAVFARCLSAIKD 296 >YHR183w [G] COG0362 6-phosphogluconate dehydrogenase Length = 489 Score = 150 bits (378), Expect = 3e-36, Method: Compositional matrix adjust. Identities = 103/301 (34%), Positives = 162/301 (53%), Gaps = 29/301 (9%) Query: 1 MIGLGKMGYNLALNLLNHGHDLVV-----SDINPQRGLQLAERGALPASSLEDMVTKLEH 55 +IGL MG NL LN +HG + S ++ + + + A+S+ED ++KL+ Sbjct: 7 LIGLAVMGQNLILNAADHGFTVCAYNRTQSKVDHFLANEAKGKSIIGATSIEDFISKLKR 66 Query: 56 PRILWVMVPAGEIVTGVIDTLAGLLEAGDIIIDGGNSHYKESIVRSGKLRERGIYLFDAG 115 PR + ++V AG V +I+ + LLE GDIIIDGGNSH+ +S R +L+++GI +G Sbjct: 67 PRKVMLLVKAGAPVDALINQIVPLLEKGDIIIDGGNSHFPDSNRRYEELKKKGILFVGSG 126 Query: 116 TSGGTEGAEHGACFMVGGDAEVFTVIEPLFRDLAV----ERGYLYSGESGSGHFLKMVHN 171 SGG EGA +G M GG E + I+ +F+ ++ E + G +G+GH++KMVHN Sbjct: 127 VSGGEEGARYGPSLMPGGSEEAWPHIKNIFQSISAKSDGEPCCEWVGPAGAGHYVKMVHN 186 Query: 172 GIEYGMMQSIAEGFELLEK--SSFDYNYENVAQLWSNGSVIRGWLMELAQNAFAKD---- 225 GIEYG MQ I E ++++++ D +V W+NG V+ +L+E+ ++ D Sbjct: 187 GIEYGDMQLICEAYDIMKRLGGFTDKEISDVFAKWNNG-VLDSFLVEITRDILKFDDVDG 245 Query: 226 -PKLSGIRGVMHSSGEGKWTVQTALDLETSTPVIALSLLMRYRSLEDDTFHGKVVAALRN 284 P + I G GKWT ALDL +I ++ R ++AL+N Sbjct: 246 KPLVEKIMDTAGQKGTGKWTAINALDLGMPVTLIGEAVFAR------------CLSALKN 293 Query: 285 E 285 E Sbjct: 294 E 294 Database: COG Posted date: Aug 3, 2010 9:58 AM Number of letters in database: 38,378,431 Number of sequences in database: 115,334 Lambda K H 0.318 0.137 0.403 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 115334 Number of Hits to DB: 23,491,775 Number of extensions: 1020203 Number of successful extensions: 2269 Number of sequences better than 1.0e-05: 57 Number of HSP's gapped: 2205 Number of HSP's successfully gapped: 57 Length of query: 296 Length of database: 38,378,431 Length adjustment: 106 Effective length of query: 190 Effective length of database: 26,153,027 Effective search space: 4969075130 Effective search space used: 4969075130 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.3 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.7 bits) S2: 115 (48.9 bits)