BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0001.0004.p (217 letters) Database: COG 115,334 sequences; 38,378,431 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value BH3587 [R] COG0546 Predicted phosphatases 151 6e-37 lin2624 [R] COG0546 Predicted phosphatases 132 6e-31 Cj1477c [R] COG0546 Predicted phosphatases 82 5e-16 NMA0828 [R] COG0546 Predicted phosphatases 80 2e-15 NMB0620 [R] COG0546 Predicted phosphatases 80 3e-15 >BH3587 [R] COG0546 Predicted phosphatases Length = 346 Score = 151 bits (382), Expect = 6e-37, Method: Compositional matrix adjust. Identities = 10/209 (4%), Positives = 35/209 (16%), Gaps = 1/209 (0%) Query: 2 IECILFDLDGTIVNTNELIINSFMHTLKENNLPPLTREQIIPYMGTTLHQQLSAFSGLED 61 I + ++ + + + E Sbjct: 3 YHRIPHSSLEVSTLGLGTMTFGEQNSEADAHAQLDYAVAQGINLIDVAEMYPVPPRP-ET 61 Query: 62 TSALELSYRSYNYAHHDELIGSFPHVNEIMEELAGRGIKLGIVTTKIRPTTIKALEMFDL 121 E ++ H +GI+ + + L Sbjct: 62 QGLTETYVGNWLAKHGSREKLIIASKVSGPSRNNDKGIRPDQALDRKNIREALHDSLKRL 121 Query: 122 LKYMETIVTVNDVVQPKPHAEPVLKAVSNLGVDPRRTLMVGDSPVDIQSXXXXXXXXXXX 181 + V+ +P + + ++ + ++ Sbjct: 122 QTDYLDLYQVHWPQRPTNCFGKLGYSWTDSAPAVSLLDTLDALAEYQRAGKIRYIGVSNE 181 Query: 182 XWSLKGEDVLRKYEPDYIIHDMKDIFGIV 210 + + D + Sbjct: 182 TAFGVMRYLHLADKHDLPRIVTIQNPYSL 210 >lin2624 [R] COG0546 Predicted phosphatases Length = 346 Score = 132 bits (331), Expect = 6e-31, Method: Compositional matrix adjust. Identities = 5/213 (2%), Positives = 26/213 (12%), Gaps = 8/213 (3%) Query: 2 IECILFDLDGTIVNTNELIINSFMHTLKENNLPP--LTREQIIPYMGTTLHQQLSAFSGL 59 T+ + + + + Sbjct: 5 RIPHSSLEVSTLGLGTMTFGEQNSEADAHA-QLDYAVAQGINLIDVAEMY---PVPPRPE 60 Query: 60 EDTSALELS--YRSYNYAHHDELIGSFPHVNEIMEELAGRGIKLGIVTTKIRPTTIKALE 117 + + + + +I S + R + Sbjct: 61 TQGLTETYVGNWLAKHGSREKLIIASKVSGPSRNNDKGIRPDQALDRKNIREALHDSLKR 120 Query: 118 MFDLLKYMETIVTVNDVVQPKPHAEPVLKAVSNLGVDPRRTLMVGDSPVDIQSXXXXXXX 177 + + + + + + + Sbjct: 121 LQTDYLDLYQVHWPQRPTNCFGKLGYSWTDSAPAVSLLDTLDALAEYQRAGKIRYIGVSN 180 Query: 178 XXXXXWSLKGEDVLRKYEPDYIIHDMKDIFGIV 210 + P + Sbjct: 181 ETAFGVMRYLHLADKHDLPRIVTIQNPYSLLNR 213 >Cj1477c [R] COG0546 Predicted phosphatases Length = 346 Score = 82.4 bits (202), Expect = 5e-16, Method: Compositional matrix adjust. Identities = 6/172 (3%), Positives = 28/172 (16%), Gaps = 3/172 (1%) Query: 1 MIECILFDLDGTIVNTNELIINSFMHTLKENNLPPLTREQIIPYMGTTLHQQLSAF--SG 58 M + + + + + L + + Sbjct: 1 MQYHRIPHSSLEVSTLGLGTMTFGEQNSEADAHAQLDYAVAQGINLIDVAEMYPVPPRPE 60 Query: 59 LEDTSALELSYRSYNYAHHDELIGSFPHVNEIMEELAGRGIKLGIVTTKIRPTTIKALEM 118 + + + + ++LI + + K + Sbjct: 61 TQGLTETYVGNWLAKHGSREKLIIASKVSGPSRN-NDKGIRPDQALDRKNIREALHDSLK 119 Query: 119 FDLLKYMETIVTVNDVVQPKPHAEPVLKAVSNLGVDPRRTLMVGDSPVDIQS 170 Y++ + + + + Sbjct: 120 RLQTDYLDLYQVHWPQRPTNCFGKLGYSWTDSAPAVSLLDTLDALAEYQRAG 171 >NMA0828 [R] COG0546 Predicted phosphatases Length = 346 Score = 80.1 bits (196), Expect = 2e-15, Method: Compositional matrix adjust. Identities = 10/220 (4%), Positives = 31/220 (14%), Gaps = 11/220 (5%) Query: 1 MIECILFDLDGTIVNTNELIINSFMHTLKENNLPPLTREQIIPYMGTTLHQQLSAFSGLE 60 M + + + + + L + + E Sbjct: 1 MQYHRIPHSSLEVSTLGLGTMTFGEQNSEADAHAQLDYAVAQGINLIDVAEMYPVPPRPE 60 Query: 61 DTSALELSYRS-------YNYAHHDELIGSFPHVNEIMEELAGRGIKLGIVTTKIRPTTI 113 E + + + ++ L + Sbjct: 61 TQGLTETYVGNWLAKHGSREKLIIASKVSG---PSRNNDKGIRPDQALDRKNIREALHDS 117 Query: 114 KALEMFDLLKYMETIVTVNDVVQPKPHAEPVLKAVSNLGVDPRRTLMVGDSPVDIQSXXX 173 D L + + + + + Sbjct: 118 LKRLQTDYLDLYQVHWPQRPTNCFGKLGYSWTDSAPAVSLLDTLDALAEYQRAGKIRYIG 177 Query: 174 XXXXXXXXXWS-LKGEDVLRKYEPDYIIHDMKDIFGIVDQ 212 L D I + + + Sbjct: 178 VSNETAFGVMRYLHLADKHDLPRIVTIQNPYSLLNRSFEV 217 >NMB0620 [R] COG0546 Predicted phosphatases Length = 346 Score = 79.7 bits (195), Expect = 3e-15, Method: Compositional matrix adjust. Identities = 11/217 (5%), Positives = 32/217 (14%), Gaps = 5/217 (2%) Query: 1 MIECILFDLDGTIVNTNELIINSFMHTLKENNLPPLTREQIIPYMGTTLHQQLSAFSGLE 60 M + + + + + L + + E Sbjct: 1 MQYHRIPHSSLEVSTLGLGTMTFGEQNSEADAHAQLDYAVAQGINLIDVAEMYPVPPRPE 60 Query: 61 DTSALELSYRSYNYAHHDELIGS----FPHVNEIMEELAGRGIKLGIVTTKIRPTTIKAL 116 E ++ H + ++ L + Sbjct: 61 TQGLTETYVGNWLAKHGSREKLIIASKVSGPSRNNDKGIRPDQALDRKNIREALHDSLKR 120 Query: 117 EMFDLLKYMETIVTVNDVVQPKPHAEPVLKAVSNLGVDPRRTLMVGDSPVDIQSXXXXXX 176 D L + + + + + Sbjct: 121 LQTDYLDLYQVHWPQRPTNCFGKLGYSWTDSAPAVSLLDTLDALAEYQRAGKIRYIGVSN 180 Query: 177 XXXXXXWS-LKGEDVLRKYEPDYIIHDMKDIFGIVDQ 212 L D I + + + Sbjct: 181 ETAFGVMRYLHLADKHDLPRIVTIQNPYSLLNRSFEV 217 Database: COG Posted date: Aug 3, 2010 9:58 AM Number of letters in database: 38,378,431 Number of sequences in database: 115,334 Lambda K H 0.319 0.138 0.399 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 115334 Number of Hits to DB: 15,119,132 Number of extensions: 590773 Number of successful extensions: 2987 Number of sequences better than 1.0e-05: 52 Number of HSP's gapped: 2962 Number of HSP's successfully gapped: 52 Length of query: 217 Length of database: 38,378,431 Length adjustment: 102 Effective length of query: 115 Effective length of database: 26,614,363 Effective search space: 3060651745 Effective search space used: 3060651745 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.4 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.7 bits) S2: 113 (48.1 bits)