BLASTP 2.2.21 [Jun-14-2009] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0001.4640.p (459 letters) Database: COGmodified 192,987 sequences; 59,019,183 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value PA2348 [C] COG2141 Coenzyme F420-dependent N5,N10-methylene tetr... 661 0.0 PA2326 [C] COG2141 Coenzyme F420-dependent N5,N10-methylene tetr... 525 e-149 Cgl0986 [C] COG2141 Coenzyme F420-dependent N5,N10-methylene tet... 477 e-134 BS_yxeK [C] COG2141 Coenzyme F420-dependent N5,N10-methylene tet... 283 5e-76 mlr9217 [C] COG2141 Coenzyme F420-dependent N5,N10-methylene tet... 264 4e-70 >PA2348 [C] COG2141 Coenzyme F420-dependent N5,N10-methylene tetrahydromethanopterin reductase and related flavin-dependent oxidoreductases Pae Pseudomonas aeruginosa Length = 462 Score = 661 bits (1706), Expect = 0.0, Method: Compositional matrix adjust. Identities = 314/459 (68%), Positives = 372/459 (81%), Gaps = 1/459 (0%) Query: 1 MAKKEIRLNAFNMNCVSHINHGLWTHPRDRSQDYTDISYWTDLARLLERGKFDGLFLADI 60 M+KK+I LNAF+MNCV HINHGLWTHPRDRS DY + +WT+LARLLERG FDGLF+ADI Sbjct: 1 MSKKQILLNAFSMNCVGHINHGLWTHPRDRSSDYRKLRHWTELARLLERGLFDGLFIADI 60 Query: 61 VGVYDVYQGNTDLTLRETIQLPVNDPLMLVSAMAAVTEHLGFGVTANLTYEAPFLFARRM 120 +GVYDVYQG ++T RE +QLPVNDPLMLVSAMA VTEHLGFGVTANLTYEAP+ FARR+ Sbjct: 61 LGVYDVYQGGIEVTAREAVQLPVNDPLMLVSAMAGVTEHLGFGVTANLTYEAPYPFARRL 120 Query: 121 STLDHLSRGRVGWNIVTGYLDSAARAMGLHEQLEHDQRYDRADEFMEVVYKLWEGSWEDG 180 STLDHLS GR+GWNIVTGYL+SAA+AMG Q+EHD+RYDRADE++EV+YKLWEGSWED Sbjct: 121 STLDHLSDGRIGWNIVTGYLESAAKAMGQSAQIEHDRRYDRADEYLEVLYKLWEGSWEDD 180 Query: 181 AVLRDRARRIYADPAKVHLISHHGDYYHVEGIHLSEPSRQRTPVLYQAGSSGRGQAFGAR 240 AVL DR RR+YA P K+H I HHG++Y VEG HL+EPS QRTPVL+QAGSS RG AF AR Sbjct: 181 AVLADRQRRVYARPEKIHKIRHHGEFYQVEGYHLAEPSPQRTPVLFQAGSSERGLAFAAR 240 Query: 241 HAECVFISSQSKDATRKLVQSIREGALREGRDAQAIKIFMGLTVVTGRTEQEAQDRYREY 300 HAECVF+S Q+++ATR+LVQ IR + GR A+ I+IFMG+ V+ T EA+D++ EY Sbjct: 241 HAECVFVSGQTREATRQLVQRIRAAVVGAGRRAEDIRIFMGINVIVAATAAEARDKHAEY 300 Query: 301 ARYANPEAGLAHFSAGTGIDFSKYGLDEPIRAAKTNAIESLTKVVTQPELNWTKRKLLQQ 360 RYA+PEAGLAHF++ +GID S+YGLDEPIR K NAIES+ K +T + T RKLL Q Sbjct: 301 RRYASPEAGLAHFASSSGIDLSRYGLDEPIRYEKNNAIESVVKNLTVARTDSTVRKLLDQ 360 Query: 361 LELGGRYASIVGAPERVADELESWVNDTGIDGYNLTRTVSPESFEDFIELVIPVLQERGS 420 L LGGRY ++VG P +VAD+LE W+ DTG+DG+NLTRTV PES+ DFI+LV+P LQ RGS Sbjct: 361 LALGGRYVTLVGDPRQVADDLEGWIADTGLDGFNLTRTVEPESYADFIDLVVPELQARGS 420 Query: 421 YKREYAQGTLREKLFQRG-ATLPPEHVGAGFRVGAAVAQ 458 YK YA GTLREKLF G A L EH GA FR A Q Sbjct: 421 YKTAYAPGTLREKLFGDGRARLQAEHAGAAFRQPPAARQ 459 >PA2326 [C] COG2141 Coenzyme F420-dependent N5,N10-methylene tetrahydromethanopterin reductase and related flavin-dependent oxidoreductases Pae Pseudomonas aeruginosa Length = 469 Score = 525 bits (1353), Expect = e-149, Method: Compositional matrix adjust. Identities = 248/450 (55%), Positives = 325/450 (72%), Gaps = 2/450 (0%) Query: 4 KEIRLNAFNMNCVSHINHGLWTHPRDRSQDYTDISYWTDLARLLERGKFDGLFLADIVGV 63 +EIRLNAF MNCV H + GLW HPRDRS Y D+ YWTDLARLLERG+FDGLF+AD++G+ Sbjct: 3 REIRLNAFEMNCVGHQSPGLWAHPRDRSSQYKDLEYWTDLARLLERGRFDGLFIADVLGI 62 Query: 64 YDVYQGNTDLTLRETIQLPVNDPLMLVSAMAAVTEHLGFGVTANLTYEAPFLFARRMSTL 123 YDV GN D +R+ Q+PVNDPL L++ MAAVT HLGFG+TA+L++E P+ FARR+STL Sbjct: 63 YDVLDGNGDAAIRQAAQVPVNDPLALITPMAAVTRHLGFGLTASLSFEHPYPFARRLSTL 122 Query: 124 DHLSRGRVGWNIVTGYLDSAARAMGLHEQLEHDQRYDRADEFMEVVYKLWEGSWEDGAVL 183 DHL++GR+GWN+VT YL+S AR +G QL+HD+RYD ADE+++V+YKL+EGSWEDGAVL Sbjct: 123 DHLTKGRIGWNVVTSYLESGARNLGQQAQLDHDRRYDYADEYLQVLYKLFEGSWEDGAVL 182 Query: 184 RDRARRIYADPAKVHLISHHGDYYHVEGIHLSEPSRQRTPVLYQAGSSGRGQAFGARHAE 243 RDR RRI++DP K+H I H G+++ V GIHL EPS QRTPVLYQAG+S RG+ F A HAE Sbjct: 183 RDRRRRIFSDPGKIHEIRHVGEHFQVPGIHLCEPSPQRTPVLYQAGASSRGKRFAAEHAE 242 Query: 244 CVFISSQSKDATRKLVQSIREGALREGRDAQAIKIFMGLTVVTGRTEQEAQDRYREYARY 303 CVF+++ SK ++ V IR+ GRD + + +F TVV G T+ +A+ ++ EY + Sbjct: 243 CVFVAAPSKTVLKQAVADIRQRVAEAGRDPRKVLVFNLQTVVLGETDAKARAKFDEYRAF 302 Query: 304 ANPEAGLAHFSAGTGIDFSKYGLDEPIRAAKTNAIESLTKVVTQ--PELNWTKRKLLQQL 361 + E LA S TGIDF +Y D+ +R TNAI+S + + P WT R+L + Sbjct: 303 VSYEGALALISGWTGIDFGQYRPDQVLRHIHTNAIQSAVEAFSSADPTRTWTVRELADWV 362 Query: 362 ELGGRYASIVGAPERVADELESWVNDTGIDGYNLTRTVSPESFEDFIELVIPVLQERGSY 421 +GG VG+P VAD L+ WV DT +DG+NL ++ E+F D +EL++P LQ+RG Y Sbjct: 363 GIGGFGPLFVGSPATVADLLQEWVEDTDVDGFNLAYALAHETFSDVVELLVPELQKRGVY 422 Query: 422 KREYAQGTLREKLFQRGATLPPEHVGAGFR 451 K EYA GTLREKLF G LP H AG+R Sbjct: 423 KTEYAPGTLREKLFGDGPRLPDSHPAAGYR 452 >Cgl0986 [C] COG2141 Coenzyme F420-dependent N5,N10-methylene tetrahydromethanopterin reductase and related flavin-dependent oxidoreductases Cgl Corynebacterium glutamicum Length = 487 Score = 477 bits (1228), Expect = e-134, Method: Compositional matrix adjust. Identities = 228/454 (50%), Positives = 310/454 (68%), Gaps = 2/454 (0%) Query: 3 KKEIRLNAFNMNCVSHINHGLWTHPRDRSQDYTDISYWTDLARLLERGKFDGLFLADIVG 62 ++I NAF+MNCV+H + GLWTHP+D+++DY + YW LA+ LE+G FDGLF+AD++G Sbjct: 11 NRKILFNAFDMNCVAHQSPGLWTHPKDKARDYNTLDYWVHLAKTLEKGLFDGLFIADVLG 70 Query: 63 VYDVYQGNTDLTLRETIQLPVNDPLMLVSAMAAVTEHLGFGVTANLTYEAPFLFARRMST 122 YDVY + + L Q+PVNDP++LVSAMA T++LGFG+TA YE P+ FARR++T Sbjct: 71 TYDVYGSSNEAALSSGAQVPVNDPILLVSAMAYATKNLGFGITAGTAYEHPYPFARRLAT 130 Query: 123 LDHLSRGRVGWNIVTGYLDSAARAMGLHEQLEHDQRYDRADEFMEVVYKLWEGSWEDGAV 182 LDHL+ GRVGWN+VTGYL SAA+ MG +QL HD+RYD+ADE++EV+YKL EGSWED AV Sbjct: 131 LDHLTNGRVGWNVVTGYLPSAAQNMGDTDQLPHDERYDKADEYLEVIYKLLEGSWEDDAV 190 Query: 183 LRDRARRIYADPAKVHLISHHGDYYHVEGIHLSEPSRQRTPVLYQAGSSGRGQAFGARHA 242 + ++ D +KVH I+HHG Y+ V GI ++EPS QRTPV+YQAG+S RG F +A Sbjct: 191 QNNTETSVFTDSSKVHAINHHGKYFDVPGIAITEPSVQRTPVIYQAGASPRGLKFAGENA 250 Query: 243 ECVFISSQSKDATRKLVQSIREGALREGRDAQAIKIFMGLTVVTGRTEQEAQDRYREYAR 302 E VFI+S + +A K V IR A+ GRD A+KIF T++TG TE +AQ + EY+R Sbjct: 251 EAVFINSSTVEAITKTVAKIRAAAVAAGRDPHAVKIFAMQTIITGETEADAQAKLEEYSR 310 Query: 303 YANPEAGLAHFSAGTGIDFSKYGLDEPIRAAKTNAIESLTKVVTQ--PELNWTKRKLLQQ 360 Y +P GL S TG D S+Y LDEPI ++NAI+S ++ E WT RKL + Sbjct: 311 YIDPVGGLTLMSGWTGADLSQYDLDEPITNIESNAIQSTAATISNGTGEGAWTVRKLGEA 370 Query: 361 LELGGRYASIVGAPERVADELESWVNDTGIDGYNLTRTVSPESFEDFIELVIPVLQERGS 420 +GG +VG+ VA EL + + +DG+NL ++P +FED ++ V+P LQ+ Sbjct: 371 TGIGGFGPVLVGSGANVAAELARIQDLSDVDGFNLAYAITPGTFEDVVDFVVPELQKLSR 430 Query: 421 YKREYAQGTLREKLFQRGATLPPEHVGAGFRVGA 454 YK EYA G+LR KL +G L H GA +R+GA Sbjct: 431 YKTEYAPGSLRNKLLGKGDRLDDTHRGASYRLGA 464 >BS_yxeK [C] COG2141 Coenzyme F420-dependent N5,N10-methylene tetrahydromethanopterin reductase and related flavin-dependent oxidoreductases Bsu Bacillus subtilis Length = 441 Score = 283 bits (725), Expect = 5e-76, Method: Compositional matrix adjust. Identities = 162/436 (37%), Positives = 241/436 (55%), Gaps = 18/436 (4%) Query: 3 KKEIRLNAFNMNCVSHINHGLWTHPRDRSQDYTDISYWTDLARLLERGKFDGLFLADIVG 62 KK+I+L F H+ W HP S ++ Y+ +LA+ ERGK D LFLAD + Sbjct: 5 KKQIKLGVFLAGTGHHV--ASWRHPDAPSDASMNLDYFKELAKTAERGKLDMLFLADSLS 62 Query: 63 VYDVYQGNTDLTLRETIQLPVNDPLMLVSAMAAVTEHLGFGVTANLTYEAPFLFARRMST 122 + N L +P L+SA+A VT +G TA+ TY PF AR+ ++ Sbjct: 63 IDSKSHPNV---------LTRFEPFTLLSALAQVTSKIGLTATASTTYSEPFHIARQFAS 113 Query: 123 LDHLSRGRVGWNIVTGYLDSAARAMGLHEQLEHDQRYDRADEFMEVVYKLWEGSWEDGAV 182 LDHLS GR GWN+VT ++S A + LEH RY RA+EF+E+V LW+ SWE+ A Sbjct: 114 LDHLSNGRAGWNVVTSSIESTALNFSGEKHLEHHLRYQRAEEFVEIVKGLWD-SWEEDAF 172 Query: 183 LRDRARRIYADPAKVHLISHHGDYYHVEGIHLSEPSRQRTPVLYQAGSSGRGQAFGARHA 242 +R++ + D K+H ++H G+Y+ V G + Q PV+ QAGSSG G+A A+ A Sbjct: 173 IRNKETGEFFDKEKMHELNHKGEYFSVRGPLNVSRTPQGQPVIIQAGSSGDGKALAAKTA 232 Query: 243 ECVFISSQSKDATRKLVQSIREGALREGRDAQAIKIFMGLTVVTGRTEQEAQDRYREYAR 302 E +F + ++ ++ QSI+E A GRD + I I G+ + TE+ AQ +Y+E Sbjct: 233 EVIFTAQNHLESAQEFYQSIKEQAAEFGRDPEKIAIMPGIFPIIADTEEAAQAKYKELQD 292 Query: 303 YANPEAGLAHFSAGT-GIDFSKYGLDEPIR---AAKTNAIESLTKVVTQ--PELNWTKRK 356 P GL GID S Y LD P+ A +NA++S K+V + N T R+ Sbjct: 293 LIIPSVGLQILQNYLGGIDLSAYPLDGPLPKLDAEASNAVKSRFKLVQEMAERDNMTIRE 352 Query: 357 LLQQLELGGRYASIVGAPERVADELESWVNDTGIDGYNLTRTVSPESFEDFIELVIPVLQ 416 L + + + VG PE++AD+++ WV+ DG+N+ + PE E F++ V+P+LQ Sbjct: 353 LYKYVAGSRGHHIFVGTPEQLADKMQEWVDTKACDGFNIMPPLLPEGIEVFVDQVVPILQ 412 Query: 417 ERGSYKREYAQGTLRE 432 ERG +++EY TLRE Sbjct: 413 ERGVFRKEYEGTTLRE 428 >mlr9217 [C] COG2141 Coenzyme F420-dependent N5,N10-methylene tetrahydromethanopterin reductase and related flavin-dependent oxidoreductases Mlo Mesorhizobium loti Length = 426 Score = 264 bits (674), Expect = 4e-70, Method: Compositional matrix adjust. Identities = 148/432 (34%), Positives = 233/432 (53%), Gaps = 11/432 (2%) Query: 6 IRLNAFNMNCVSHINHGLWTHPRDRSQDYTDISYWTDLARLLERGKFDGLFLADIVGVYD 65 ++ AF + + +H +W HP+ ++ + D ++W L R LE G+FDGLF AD++ ++ Sbjct: 1 MKFVAFLLAGPTCHHHAMWRHPQTENR-FLDPAFWETLGRTLEAGRFDGLFFADVLSFHN 59 Query: 66 VYQGNTDLTLRETIQLPVNDPLMLVSAMAAVTEHLGFGVTANLTYEAPFLFARRMSTLDH 125 + T+ Q+ + DP+ LV+ MA T +G GVT + T++ P+ AR +S+LD Sbjct: 60 ------ETTMTRGGQMSLLDPVPLVAMMARATSRIGLGVTLSTTFQDPYAIARVLSSLDV 113 Query: 126 LSRGRVGWNIVTGYLDSAARAMGLHEQLEHDQRYDRADEFMEVVYKLWEGSWEDGAVLRD 185 LS GR+ WNIVT + D A+ G L +RY+RA+E ++V LW G W DGA++ D Sbjct: 114 LSGGRIAWNIVTSFSDLDAQIFGEEALLPRSERYERAEEVLKVCLDLWTG-WADGALVLD 172 Query: 186 RARRIYADPAKVHLISHHGDYYHVEGIHLSEPSRQRTPVLYQAGSSGRGQAFGARHAECV 245 + + D AKV G Y G+ PS QR PV+ QAGSS RG+ F A HAE V Sbjct: 173 KESGDFIDEAKVRRTEIAGKYVGARGVFTVPPSPQRHPVIMQAGSSPRGRDFAASHAELV 232 Query: 246 FISSQSKDATRKLVQSIREGALREGRDAQAIKIFMGLTVVTGRTEQEAQDRYREYARYAN 305 F S ++ +++ R GR+ I + + + G T A+++ + Sbjct: 233 FTLQHSLPDMQEFYAEMKDRVGRAGRNPDDCAILVSVDPIVGETNSLAREKQAYINDLVD 292 Query: 306 PEAGLAHFSAGTGIDFSKYGLDEPI--RAAKTNAIESLTKVVTQPEL-NWTKRKLLQQLE 362 P G+ S TG+D S+Y +D+PI A+T + S ++ + N T + + Sbjct: 293 PHVGIPFMSDHTGLDLSQYPVDKPIIDIPAETGSRGSFDIILQGTKANNLTLGEAAKHFA 352 Query: 363 LGGRYASIVGAPERVADELESWVNDTGIDGYNLTRTVSPESFEDFIELVIPVLQERGSYK 422 + +VG PE VAD+L+ + + G DG+ +T T P SFE F V+P+LQ+RG ++ Sbjct: 353 VSELCPQVVGTPETVADQLQDFFENKGCDGFIVTPTEMPGSFEAFTRSVVPILQKRGLFR 412 Query: 423 REYAQGTLREKL 434 +EY TLRE + Sbjct: 413 KEYPGSTLRETI 424