BLASTP 2.2.21 [Jun-14-2009] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0001.4585.p (402 letters) Database: COGmodified 192,987 sequences; 59,019,183 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value SMb21000 [GEPR] COG0477 Permeases of the major facilitator super... 92 3e-18 AGc4193 [GEPR] COG0477 Permeases of the major facilitator superf... 77 8e-14 mlr7399 [GEPR] COG0477 Permeases of the major facilitator superf... 72 2e-12 BMEI1555 [GEPR] COG0477 Permeases of the major facilitator super... 66 2e-10 yjiJ [GEPR] COG0477 Permeases of the major facilitator superfami... 59 3e-08 >SMb21000 [GEPR] COG0477 Permeases of the major facilitator superfamily Sme Sinorhizobium meliloti Length = 377 Score = 91.7 bits (226), Expect = 3e-18, Method: Compositional matrix adjust. Identities = 112/355 (31%), Positives = 163/355 (45%), Gaps = 20/355 (5%) Query: 29 AVALGLARFSYALLLAPMRADLGWPYLVAGGMNTGNALGYLLGALSTPLLMRRFQAHRVL 88 AVA+G RFSY +L M D G AG + + N +GYL GA+ + V Sbjct: 2 AVAMGFGRFSYTPILPAMMTDTGLSPADAGLVASANFIGYLAGAVLAGYRWAHGRERGVG 61 Query: 89 IAGAFGTALLSFLPALTTDTLWQLLLRIVTGVTS--ALIFVSGGLLVSRLAALHAQRAGL 146 +A T LL LT+ L ++R + G+ S A+IF+SG +L LAA R+ Sbjct: 62 LAALVATTLLLAAMGLTSSVLAYSIIRFLAGLASAFAMIFISGIVLGQGLAA----RSEH 117 Query: 147 LLGLYYGGTGIGIVAASLLVPPVMQAAVAQGAPHPWQWAWVLLGSLALVASVCMALPARL 206 + +++GG G GI +S+ V A VA + W +G ALVA L A L Sbjct: 118 VPSVHFGGVGFGIALSSIAVWVAPLAGVAGLSMSQADW---FVG--ALVALAGTVLVAAL 172 Query: 207 MPTAPAQAKGARHW----RPRSLGFGLVGYLFFGLGYIGYMTFVIALLKEEGMSASRITL 262 +P + A G R R R L + Y FFG GY+ TF++A+ ++ S L Sbjct: 173 LPASRHAANGGRPEVPLVRSRPLMAMTLTYGFFGFGYVITATFLVAMARDASGGHSVEFL 232 Query: 263 FFALLGAATFASSRIWAPMLDRFRGGQSLAILNGLLCLAT--LLPALTAEPLAVLASGLL 320 + + G + S +W + RF AI GLL A +L P A L GL+ Sbjct: 233 AWLITGVSAALSVYLWRFAVPRFGLAGVYAI--GLLVEAVGLVLTVSLPSPYAPLVGGLM 290 Query: 321 FGGCFLSAVASTTAMVRHNLPPADWPAGISAFTIIFAVGQIIGPSIVGWIADGSG 375 G F+ A + R L P ++ T F +GQI+GP + GW+A+ +G Sbjct: 291 LGATFMMVTAYGLQIGRQ-LAPESPRRALAFMTAAFGIGQIVGPLVAGWLAERTG 344 >AGc4193 [GEPR] COG0477 Permeases of the major facilitator superfamily Atu Agrobacterium tumefaciens strain C58 (Cereon) Length = 396 Score = 76.6 bits (187), Expect = 8e-14, Method: Compositional matrix adjust. Identities = 107/374 (28%), Positives = 161/374 (43%), Gaps = 16/374 (4%) Query: 8 VPSPFIRRHPILTALALSLGTAVALGLARFSYALLLAPMRADLGWPYLVAGGMNTGNALG 67 + P RH A +L A A+G RFS+ +L M AD+ AG + GN G Sbjct: 3 IDKPLSTRHLTALGFAGALMMASAMGFGRFSFTPILPGMMADVPLSAGDAGIVAAGNFAG 62 Query: 68 YLLGALSTPLLMRRFQAHRVLIAGAFGTALLSFLPALTTDTLWQLLLRIVTGVTSALIFV 127 YL GA+ + V + G F TA L A ++R + GV SA+ + Sbjct: 63 YLAGAILAASSWGSGRERLVALCGLFSTAALLLAMAAFNSVAAFTVIRFLAGVASAMTMI 122 Query: 128 SGGLLVSRLAALHAQRAGL--LLGLYYGGTGIGIVAASLLVPPVMQAAVAQGAPHPWQWA 185 S++ HA +AG + L+YGG G GI +SL V + V G W+ Sbjct: 123 ----FTSQIVIGHAAKAGRDSVQALHYGGVGAGIAISSLAV--YLIGVVFDGGGSSWREE 176 Query: 186 WVLLGSLALVASVCMALPARLMPTAPAQAKGARHWRP----RSLGFGLVGYLFFGLGYIG 241 W+ + A+ + V L R++P P + + P R L + Y FG GY+ Sbjct: 177 WI---AGAVFSFVAFLLVFRVLPAGPPRHASSAAEPPLAWTRPLFLTTLAYGLFGFGYVI 233 Query: 242 YMTFVIALLKEEGMSASRITLFFALLGAATFASSRIWAPMLDRFRGGQSLAILNGLLCLA 301 TF++A+ + A L + + G A AS +W P+L R ++ I+ + + Sbjct: 234 TATFIVAIARMAAAGAFVEFLAWFITGCAAAASLFLWKPVLKSQRLKRAFVIVLAVEAVG 293 Query: 302 TLLPALTAEPLAVLASGLLFGGCFLSAVASTTAMVRHNLPPADWPAGISAFTIIFAVGQI 361 + VLA GLL G F+ A + R P+ A + T F GQI Sbjct: 294 VFASVMLPPVAGVLAGGLLLGLTFMVITAYGLQLGREFAGPSPRRA-FALMTAAFGTGQI 352 Query: 362 IGPSIVGWIADGSG 375 +GP GWIA+ +G Sbjct: 353 VGPLAAGWIAEVTG 366 >mlr7399 [GEPR] COG0477 Permeases of the major facilitator superfamily Mlo Mesorhizobium loti Length = 401 Score = 72.0 bits (175), Expect = 2e-12, Method: Compositional matrix adjust. Identities = 106/395 (26%), Positives = 172/395 (43%), Gaps = 22/395 (5%) Query: 15 RHPILTALALS----LGTAVALGLARFSYALLLAPMRADLGWPYLVAGGMNTGNALGYLL 70 R TAL S + AVA+G+ RF Y +L M +L AG + + N LGYL+ Sbjct: 17 RSATSTALRFSVAGMIAMAVAMGIGRFVYTPILPGMMEELHLSPANAGWIASANYLGYLV 76 Query: 71 GALSTPLLMRRFQAHRVLIAGAFGTALLSFLPALTTDTLWQLLLRIVTGVTSALIFVSGG 130 GA + + +++ G + +L+ L LT LL+R + G+ SA + V Sbjct: 77 GAFAAAGGWAHGRERLLMLGGLGASTILAALMGLTDAMAAFLLIRFLAGLASAFVMVFMA 136 Query: 131 LLVSRLAALHAQRAGLLLGLYYGGTGIGIVAASLLVPPVMQAAVAQGAPHP-WQWAWVLL 189 +V + ++A G L ++GG G+GI + VM A + H W W+ Sbjct: 137 SIV--FSHINAAGRGDLQAWHFGGVGLGIA-----ISAVMMAVLVT--EHAGWAAGWLWS 187 Query: 190 GSLALVASVCMALPARLMPTAPAQA-KGARHWRPRSLGFGLVGYLFFGLGYIGYMTFVIA 248 +++ V +AL A P A QA + + RSL ++ Y FG GY+ TF++A Sbjct: 188 AAISACGFVVVALLANEGPLANGQAVREPALPKDRSLTKIIIAYGLFGFGYVVTATFLVA 247 Query: 249 LLKEEGMSASRITLFFALLGAATFASSRIWAPMLDRFRGGQSLAILNGLLCLATLLPALT 308 ++++ G S + + + G A F S+ +W + G L L CL ++ Sbjct: 248 IVRQGGGSRVFEAMVWLVAGLAGFPSTWLW----QKIAGRVGLNAAYALACLIEVIGVTA 303 Query: 309 AEPLAVLASGLLFGGCFLSAVASTTAMVRHNLPPADWPAGISAFTII---FAVGQIIGPS 365 + L + LL G + TA+ A F ++ F +GQIIGP Sbjct: 304 SVALGGYSGPLLGGLLLGGTFIAITALGLQAARQQAPQAPRRIFALMTASFGLGQIIGPI 363 Query: 366 IVGWIADGSGGLQRGLVFSALMLLAGSMVAWRQRP 400 G +A SG + +A ML+ ++ W P Sbjct: 364 AAGLLAQMSGDFFLASIVAAAMLVVSGVITWSAAP 398 >BMEI1555 [GEPR] COG0477 Permeases of the major facilitator superfamily Bme Brucella melitensis Length = 272 Score = 65.9 bits (159), Expect = 2e-10, Method: Compositional matrix adjust. Identities = 70/231 (30%), Positives = 111/231 (48%), Gaps = 19/231 (8%) Query: 29 AVALGLARFSYALLLAPMRADLGWPYLVAGGMNTGNALGYLLGALSTPLLMRRFQAHRVL 88 A A+G+ RF Y +L M AD+G+ AG + + N +GYL+GA+ V+ Sbjct: 2 AAAMGVGRFIYTPILPGMMADIGFSTADAGFIASANYIGYLVGAVVAGYGWSAGIERMVV 61 Query: 89 IAGAFGTALLSFLPALTTDTLWQL-LLRIVTGVTSALIFV-SGGLLVSRLAALHAQRAGL 146 IAG +A+ F AL D +W L +LR G SA+ + +++S + AQRAG Sbjct: 62 IAGLAASAVFCFAMAL-FDGVWILSILRFAAGFASAVTMILCTAIVLSHIT--RAQRAG- 117 Query: 147 LLGLYYGGTGIGIVAASLLVPPVMQAAVAQGAPHPWQWAWVLLGSLALVASVCMALPARL 206 L +++GG G GI ++LLV + + + W+ W+ G L L V MA+ Sbjct: 118 LGAMHFGGVGSGITLSALLVALIRFSGLG------WRADWIGAGLLTL---VIMAVVTAF 168 Query: 207 MPTAPAQAKGARHW--RPRSLGFGLVGYLF--FGLGYIGYMTFVIALLKEE 253 + P P S F + + + FG GY+ TF+IA+++ Sbjct: 169 VKEGPVGGNNGMREPPLPNSFAFHALAFAYGIFGFGYVITATFLIAIVRAN 219 >yjiJ [GEPR] COG0477 Permeases of the major facilitator superfamily Eco Escherichia coli K12 Length = 392 Score = 58.5 bits (140), Expect = 3e-08, Method: Compositional matrix adjust. Identities = 93/377 (24%), Positives = 160/377 (42%), Gaps = 34/377 (9%) Query: 13 IRRHPILTALALSLGTAVALGLARFSYALLLAPMRADLGWPYLVAGGMNTGNALGYL--- 69 + R TAL L + +GL RF Y +L M A+ + + + +GN GYL Sbjct: 8 VERFSFSTALFGMLVLTLGMGLGRFLYTPMLPVMMAEGSFSFSQLSWIASGNYAGYLAGS 67 Query: 70 ----LGALSTPLLMRRFQAHRVLIAGAFGTALLSFLPALTTDTLWQLLLRIVTGVTSALI 125 GA P +R F L+A A + LL A + LL+R++ GV SA + Sbjct: 68 LLFSFGAFHQPSRLRPF-----LLASALASGLLILAMAWLPPFILVLLIRVLAGVASAGM 122 Query: 126 FVSGGLLVSRLAALHAQRAGLLLGLYYGGTGIGIVAASLLVPPVMQAAVAQGAPHPWQWA 185 + G L+ + R +L + G GIGI + V + ++ + WQ A Sbjct: 123 LIFGSTLI-----MQHTRHPFVLAALFSGVGIGIALGNEYVLAGLHFDLS--SQTLWQGA 175 Query: 186 WVLLGSLALVASVCMALPARL-----MPTAPAQAKGARHWRPRSLGFGLVGYLFFGLGYI 240 L G + + ++ M P++ MP A + + W ++ +GL G+ GYI Sbjct: 176 GALSGMMLIALTLLM--PSKKHAITPMPLAKTEQQ-IMSWWLLAILYGLAGF-----GYI 227 Query: 241 GYMTFVIALLKEEGMSASRITLFFALLGAATFASSRIWAPMLDRFRGGQSLAILNGLLCL 300 T++ + K+ G S + L+G + W R+ L + + Sbjct: 228 IVATYLPLMAKDAG-SPLLTAHLWTLVGLSIVPGCFGWLWAAKRWGALPCLTANLLVQAI 286 Query: 301 ATLLPALTAEPLAVLASGLLFGGCFLSAVASTTAMVRHNLPPADWPAGISAFTIIFAVGQ 360 LL + PL ++ S L FGG F+ + + R P + + T+I+ +GQ Sbjct: 287 CVLLTLASDSPLLLIISSLGFGGTFMGTTSLVMTIARQLSVPGNLNL-LGFVTLIYGIGQ 345 Query: 361 IIGPSIVGWIADGSGGL 377 I+GP++ +++G+ L Sbjct: 346 ILGPALTSMLSNGTSAL 362