BLASTP 2.2.21 [Jun-14-2009] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0001.4580.p (324 letters) Database: COGmodified 192,987 sequences; 59,019,183 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value RSc0255 [R] COG2070 Dioxygenases related to 2-nitropropane dioxy... 446 e-125 CC2372 Non COG 390 e-108 SMc02150 [R] COG2070 Dioxygenases related to 2-nitropropane diox... 375 e-104 AGc729 [R] COG2070 Dioxygenases related to 2-nitropropane dioxyg... 373 e-103 RSp0316 [R] COG2070 Dioxygenases related to 2-nitropropane dioxy... 372 e-103 >RSc0255 [R] COG2070 Dioxygenases related to 2-nitropropane dioxygenase Rso Ralstonia solanacearum Length = 327 Score = 446 bits (1147), Expect = e-125, Method: Compositional matrix adjust. Identities = 232/324 (71%), Positives = 277/324 (85%), Gaps = 1/324 (0%) Query: 1 MPSAPAYPQALQDLRLPIICSPMFIASGPALVAAQCKAGLVGSFPALNARPAELLDTWLS 60 MP+A P ALQ L LP+I SPMFI S P LV AQCKAG+VG+FPALNARPAE+LD WL+ Sbjct: 1 MPAAKHLPAALQHLSLPVIGSPMFIVSYPELVLAQCKAGIVGAFPALNARPAEVLDDWLT 60 Query: 61 QLQEELAEWQQAHPQRKVGPIAVNQIVHQSNDRLAQDVATCVKHQVPIIISSLRAPPKEM 120 Q+ ELA + AHP VGP+AVNQIVH SN RL QDVATCVKH+VPI I+SLRAP +E+ Sbjct: 61 QINAELAAHRAAHPHAVVGPLAVNQIVHASNARLEQDVATCVKHKVPIFITSLRAPVREI 120 Query: 121 LDAVHSYGGIVLHDVISLRHAEKALEAGVDGLILVAAGAGGHAGTLSPFALVGEVRKMFK 180 LDAVHSYGGIVLHDVI+LRHAEKALEAGVDGLILVAAGAGGHAGTLSPFALVGEVR++F Sbjct: 121 LDAVHSYGGIVLHDVINLRHAEKALEAGVDGLILVAAGAGGHAGTLSPFALVGEVRRIFN 180 Query: 181 GPLVLSGAIATGDAVLAAQAMGADFAYMGSRWLATRESNVDEAYRQAIVESTAADVVYTN 240 GP+ LSGAIATGDA+LAAQAMGAD AY+G+R++A++E++ AY++AI+ + A+D+VYT+ Sbjct: 181 GPIALSGAIATGDAILAAQAMGADLAYIGTRFIASQEAHAAGAYKEAILRAAASDIVYTS 240 Query: 241 LFTGVHGNYLKKSIVNAGLDPDNLPEADKSKMNFGSGGGSKAKAWRDIWGAGQGVGLMQD 300 LFTGVHGNY+++SI AGLDP+ LPEADKSKMNFG G +KA+AW+DIWGAGQGVG + D Sbjct: 241 LFTGVHGNYIRESIELAGLDPEALPEADKSKMNFGD-GSAKARAWKDIWGAGQGVGQISD 299 Query: 301 VPSVAEMVDRLEAEYRAARARLAL 324 +P E+V RL+AEY AA+ RLAL Sbjct: 300 LPPAGEIVTRLKAEYEAAKLRLAL 323 >CC2372 Non COG Length = 335 Score = 390 bits (1003), Expect = e-108, Method: Compositional matrix adjust. Identities = 196/319 (61%), Positives = 251/319 (78%), Gaps = 2/319 (0%) Query: 6 AYPQALQD-LRLPIICSPMFIASGPALVAAQCKAGLVGSFPALNARPAELLDTWLSQLQE 64 A P L+D LRLP+I SP+FI S P LV AQCKAG+VGSFP+LNARP LD WL+++ E Sbjct: 2 ALPPILRDRLRLPVIASPLFIISNPDLVIAQCKAGIVGSFPSLNARPLSQLDEWLARITE 61 Query: 65 ELAEWQQAHPQRKVGPIAVNQIVHQSNDRLAQDVATCVKHQVPIIISSLRAPPKEMLDAV 124 ELA W +A+P P AVNQIVH++N+RL D+A CVK++VP++I+SL A +++ A+ Sbjct: 62 ELAAWDKANPDMPSAPFAVNQIVHKTNNRLEDDIAMCVKYKVPLVITSLGAR-EDVNQAI 120 Query: 125 HSYGGIVLHDVISLRHAEKALEAGVDGLILVAAGAGGHAGTLSPFALVGEVRKMFKGPLV 184 HSYGGI LHDVI+ R A KA+E G DGLI VAAGAGGHAGTLSPFAL+ E+R+ F GP+ Sbjct: 121 HSYGGITLHDVITDRFARKAIEKGADGLIPVAAGAGGHAGTLSPFALIQEIRQWFDGPVA 180 Query: 185 LSGAIATGDAVLAAQAMGADFAYMGSRWLATRESNVDEAYRQAIVESTAADVVYTNLFTG 244 LSG+IA+G ++LAAQAMGAD AY+GS ++AT+E+N E Y+Q IV+S A D++Y+NLFTG Sbjct: 181 LSGSIASGRSILAAQAMGADLAYVGSAFIATKEANAVEGYKQMIVDSKADDILYSNLFTG 240 Query: 245 VHGNYLKKSIVNAGLDPDNLPEADKSKMNFGSGGGSKAKAWRDIWGAGQGVGLMQDVPSV 304 VHGNYL+ S++ AGLDP+NLPE+D SKMNFGSGG AKAWRDIWG GQG+G + +V + Sbjct: 241 VHGNYLRPSVIKAGLDPENLPESDPSKMNFGSGGNQDAKAWRDIWGCGQGIGAIDNVLTA 300 Query: 305 AEMVDRLEAEYRAARARLA 323 E+V + AEY A+A LA Sbjct: 301 GELVAKFAAEYEQAKAELA 319 >SMc02150 [R] COG2070 Dioxygenases related to 2-nitropropane dioxygenase Sme Sinorhizobium meliloti Length = 336 Score = 375 bits (963), Expect = e-104, Method: Compositional matrix adjust. Identities = 197/310 (63%), Positives = 243/310 (78%), Gaps = 2/310 (0%) Query: 15 RLPIICSPMFIASGPALVAAQCKAGLVGSFPALNARPAELLDTWLSQLQEELAEWQQAHP 74 RLP+I +P+FI S P L AQCKAG++G+FPALNARP LD WL+++ E LA+ HP Sbjct: 12 RLPVIGAPLFIVSHPRLTIAQCKAGIIGAFPALNARPQSQLDEWLAEITETLADHDAGHP 71 Query: 75 QRKVGPIAVNQIVHQSNDRLAQDVATCVKHQVPIIISSLRAPPKEMLDAVHSYGGIVLHD 134 +R P AVNQIVH+SN RL QD+ CVK++VPI+ISSL A P E+ A+HSYGG+VLHD Sbjct: 72 ERPAAPFAVNQIVHKSNARLEQDLMLCVKYKVPIVISSLGAVP-EVNAAIHSYGGVVLHD 130 Query: 135 VISLRHAEKALEAGVDGLILVAAGAGGHAGTLSPFALVGEVRKMFKGPLVLSGAIATGDA 194 VI+ RHA A+ G DGLI VAAGAGGHAGTLSPFALV E+R F GPL+LSGAIATGDA Sbjct: 131 VINNRHANSAIRKGADGLIAVAAGAGGHAGTLSPFALVQEIRAWFDGPLLLSGAIATGDA 190 Query: 195 VLAAQAMGADFAYMGSRWLATRESNVDEAYRQAIVESTAADVVYTNLFTGVHGNYLKKSI 254 +LAAQAMGAD AY+GS ++AT E+ AY+Q IV+S AAD+VY+N FTG+HGNYL+ SI Sbjct: 191 ILAAQAMGADMAYIGSPFIATEEARASAAYKQMIVDSDAADIVYSNYFTGIHGNYLRASI 250 Query: 255 VNAGLDPDNLPEADKSKMNFGSGGGSKAKAWRDIWGAGQGVGLMQDVPSVAEMVDRLEAE 314 + AG+DP NLPEAD SKM+FG+ AKAW+DIWG GQG+G +++V +VA +VDRL+ E Sbjct: 251 LAAGMDPANLPEADPSKMDFGT-ATEGAKAWKDIWGCGQGIGAVREVGTVARLVDRLDQE 309 Query: 315 YRAARARLAL 324 Y AARARL Sbjct: 310 YDAARARLGF 319 >AGc729 [R] COG2070 Dioxygenases related to 2-nitropropane dioxygenase Atu Agrobacterium tumefaciens strain C58 (Cereon) Length = 324 Score = 373 bits (957), Expect = e-103, Method: Compositional matrix adjust. Identities = 197/316 (62%), Positives = 246/316 (77%), Gaps = 3/316 (0%) Query: 8 PQALQD-LRLPIICSPMFIASGPALVAAQCKAGLVGSFPALNARPAELLDTWLSQLQEEL 66 P L+D LRLP+I SP+FI S P L AQCKAG++G+FPALNARP LD WL+ + EEL Sbjct: 7 PSVLKDNLRLPVIASPLFIISHPQLTLAQCKAGVIGAFPALNARPESQLDEWLAMITEEL 66 Query: 67 AEWQQAHPQRKVGPIAVNQIVHQSNDRLAQDVATCVKHQVPIIISSLRAPPKEMLDAVHS 126 A + +A+P+R P AVNQIVH SN RL D+ CVK++VP++ISSL A P E+ AVHS Sbjct: 67 AAYNRANPERPAAPFAVNQIVHMSNRRLEHDLGLCVKYKVPVVISSLGAVP-EVNAAVHS 125 Query: 127 YGGIVLHDVISLRHAEKALEAGVDGLILVAAGAGGHAGTLSPFALVGEVRKMFKGPLVLS 186 YGGIVLHDVI+ RHA A+ G DGLI VAAGAGGHAGTLSPFAL+ E+R+ F GPL+L+ Sbjct: 126 YGGIVLHDVINDRHARSAIRKGADGLIAVAAGAGGHAGTLSPFALIQEIREWFDGPLLLA 185 Query: 187 GAIATGDAVLAAQAMGADFAYMGSRWLATRESNVDEAYRQAIVESTAADVVYTNLFTGVH 246 GAIA G ++LAAQAMGAD AY+GS ++AT E+ + Y+QA+V++ A D+VY+N FTGVH Sbjct: 186 GAIANGGSILAAQAMGADMAYIGSPFIATEEARASDGYKQALVDAQAKDIVYSNYFTGVH 245 Query: 247 GNYLKKSIVNAGLDPDNLPEADKSKMNFGSGGGSKAKAWRDIWGAGQGVGLMQDVPSVAE 306 GNYLK SIV +G+DPDNLPEAD SKM+F + G AKAW+DIWGAGQG+G ++ V VA Sbjct: 246 GNYLKSSIVASGMDPDNLPEADPSKMDFETATGG-AKAWKDIWGAGQGIGAIKAVEPVAA 304 Query: 307 MVDRLEAEYRAARARL 322 +VDRL AEY AA+ R+ Sbjct: 305 LVDRLAAEYEAAKGRI 320 >RSp0316 [R] COG2070 Dioxygenases related to 2-nitropropane dioxygenase Rso Ralstonia solanacearum Length = 335 Score = 372 bits (955), Expect = e-103, Method: Compositional matrix adjust. Identities = 207/316 (65%), Positives = 253/316 (80%), Gaps = 2/316 (0%) Query: 8 PQALQD-LRLPIICSPMFIASGPALVAAQCKAGLVGSFPALNARPAELLDTWLSQLQEEL 66 P LQ+ L +P++ SP+FI S P LV AQCKAG+VGSFPALNARPAELL+TWL ++ L Sbjct: 4 PPILQNRLSVPVVGSPLFIISNPDLVIAQCKAGVVGSFPALNARPAELLETWLQRITTAL 63 Query: 67 AEWQQAHPQRKVGPIAVNQIVHQSNDRLAQDVATCVKHQVPIIISSLRAPPKEMLDAVHS 126 AE+ HP + P AVNQIVH+SNDRL D+A CVK++VPI+I+SL A +E+ AVHS Sbjct: 64 AEYDAQHPDKPSAPFAVNQIVHKSNDRLEHDLALCVKYKVPIVITSLGAR-EEVNHAVHS 122 Query: 127 YGGIVLHDVISLRHAEKALEAGVDGLILVAAGAGGHAGTLSPFALVGEVRKMFKGPLVLS 186 YGGIVLHDVI+ A+KA++ G DGLI VAAGAGGHAGT SPFAL+ E+R+ F GPL+LS Sbjct: 123 YGGIVLHDVINNTFAKKAIDKGADGLIAVAAGAGGHAGTTSPFALIHEIREWFDGPLLLS 182 Query: 187 GAIATGDAVLAAQAMGADFAYMGSRWLATRESNVDEAYRQAIVESTAADVVYTNLFTGVH 246 GAIA G+A+LAA A GAD AY+GS ++AT E+N EAY+QAIVESTA+D++YTNLFTGVH Sbjct: 183 GAIANGNAILAALAAGADLAYVGSAFIATEEANAIEAYKQAIVESTASDIIYTNLFTGVH 242 Query: 247 GNYLKKSIVNAGLDPDNLPEADKSKMNFGSGGGSKAKAWRDIWGAGQGVGLMQDVPSVAE 306 GNYL+KSIV +GLDPD LPE+D SKMNFGSG G+KAKAW+DIWGAGQGVG ++ V AE Sbjct: 243 GNYLRKSIVASGLDPDALPESDPSKMNFGSGDGAKAKAWKDIWGAGQGVGAVKRVVPAAE 302 Query: 307 MVDRLEAEYRAARARL 322 +V R E+ AR RL Sbjct: 303 LVRRFAKEFEMARRRL 318