BLASTP 2.2.21 [Jun-14-2009] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0001.4566.p (200 letters) Database: COGmodified 192,987 sequences; 59,019,183 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value RSc0273 [Q] COG3917 2-hydroxychromene-2-carboxylate isomerase Rs... 211 7e-55 PA0118 [Q] COG3917 2-hydroxychromene-2-carboxylate isomerase Pae... 114 1e-25 CC0643 Non COG 90 3e-18 AGpA622 [Q] COG3917 2-hydroxychromene-2-carboxylate isomerase At... 60 3e-09 AGc3262 [Q] COG2761 Predicted dithiol-disulfide isomerase involv... 51 1e-06 >RSc0273 [Q] COG3917 2-hydroxychromene-2-carboxylate isomerase Rso Ralstonia solanacearum Length = 201 Score = 211 bits (537), Expect = 7e-55, Method: Compositional matrix adjust. Identities = 99/201 (49%), Positives = 136/201 (67%), Gaps = 1/201 (0%) Query: 1 MSNPIDFYFDFSSPYGYFAAVEIDKLAARHGRSVNWHPILLGPIFKALGTNSLVNIPLKG 60 M+ IDFYFDFSSPYGYFA+ ++ LA ++ R+ WHPILLG +FK G L PLKG Sbjct: 1 MNGAIDFYFDFSSPYGYFASTRVEALAQQYNRATAWHPILLGVVFKTTGGAPLAQQPLKG 60 Query: 61 EYSKHDMERTARFHNIFYQAPSNFPIGSQVAARATLWVQQTQPA-KAVDLIKTLYSAYFT 119 +Y+ D ERTARFH I Y+ P++FP+ +Q AARA LWV +A+ + +Y A F Sbjct: 61 DYAWRDFERTARFHGIDYRKPTHFPLPTQYAARAMLWVHDHHGGDRAIGFAQAVYRALFV 120 Query: 120 EDIDISVTDHVLRIAADAGIDRHALEAALASPELKEQLRQRTDAAAKAGVFGSPFMIVDG 179 +D++I V++IA GID +AL A S ++K+QL+ D A GVFGSP++I+DG Sbjct: 121 DDVNIGEPAEVMKIADALGIDGNALNAGAGSQQIKDQLKAEIDLAMSRGVFGSPYVIIDG 180 Query: 180 EAFWGFDRFTQLEAFLKNGKI 200 E FWGFDRF Q+EA L++G++ Sbjct: 181 EPFWGFDRFDQIEALLRDGRV 201 >PA0118 [Q] COG3917 2-hydroxychromene-2-carboxylate isomerase Pae Pseudomonas aeruginosa Length = 195 Score = 114 bits (286), Expect = 1e-25, Method: Compositional matrix adjust. Identities = 63/191 (32%), Positives = 102/191 (53%), Gaps = 8/191 (4%) Query: 1 MSNPIDFYFDFSSPYGYFAAVEIDKLAARHGRSVNWHPILLGPIFKALGTNSLVNIPLKG 60 MS I+F+FDF SP Y A ++ ++AA HG S+ W P+LLG +FKA G +S + +P KG Sbjct: 1 MSKQIEFFFDFGSPTTYLAWTQLPRIAAAHGASIAWRPMLLGGVFKATGNHSPIEVPAKG 60 Query: 61 EYSKHDMERTARFHNIFYQAPSNFPIGSQV---AARATLWVQQTQPAKAVDLIKTLYSAY 117 Y+ HD+ R A+ + + FPI + A+ L + QP +K ++ A Sbjct: 61 RYTLHDLARYAKRYGVPLAFNPAFPINTLTLMRGAQGYLGGEGFQP-----YLKAVFEAL 115 Query: 118 FTEDIDISVTDHVLRIAADAGIDRHALEAALASPELKEQLRQRTDAAAKAGVFGSPFMIV 177 + ++ + V ++ A+AG D + ++KE L+ T+ A + GVFG+P V Sbjct: 116 WVRQQNLGKPEVVAQVLAEAGFDPDEFLRLVGDEQVKEGLKATTEEAVRRGVFGAPSFFV 175 Query: 178 DGEAFWGFDRF 188 + F+G DR Sbjct: 176 GDQLFFGQDRL 186 >CC0643 Non COG Length = 206 Score = 89.7 bits (221), Expect = 3e-18, Method: Compositional matrix adjust. Identities = 67/203 (33%), Positives = 99/203 (48%), Gaps = 9/203 (4%) Query: 2 SNPIDFYFDFSSPYGYFAAVEIDKLAARHGRSVNWHPILLGPIFKALGTNSLVNIPLKGE 61 + IDF FDF SP Y + + ++AAR G +V P LLG IFKA G N I G Sbjct: 7 NKSIDFIFDFGSPNAYLSWKLLPEIAARQGAAVRLIPCLLGGIFKATG-NQAPMIAFGGV 65 Query: 62 YSKHDME--RTARF---HNIF-YQAPSNFPIGSQVAARATLWVQQTQPAKAVDLIKTLYS 115 K D E T RF H + ++ +FP+ + + R + Q+ A+A ++ + Sbjct: 66 KGKMDYEMLETRRFIAAHGLTAFRFNPHFPVNTLLLMRGMIAAQRIGVAEAY--LEAMLK 123 Query: 116 AYFTEDIDISVTDHVLRIAADAGIDRHALEAALASPELKEQLRQRTDAAAKAGVFGSPFM 175 + + + + + + A AG+D AL AA E+K +L T+AA G FG P Sbjct: 124 GMWEDGLKLDDPEVFVATANAAGLDGAALLAATGDAEVKAELVANTEAAVARGTFGIPTF 183 Query: 176 IVDGEAFWGFDRFTQLEAFLKNG 198 V E F+G +R Q+EA L G Sbjct: 184 FVGEEIFFGKERLGQVEAELAKG 206 >AGpA622 [Q] COG3917 2-hydroxychromene-2-carboxylate isomerase Atu Agrobacterium tumefaciens strain C58 (Cereon) Length = 219 Score = 60.1 bits (144), Expect = 3e-09, Method: Compositional matrix adjust. Identities = 49/191 (25%), Positives = 79/191 (41%), Gaps = 6/191 (3%) Query: 7 FYFDFSSPYGYFAAVEIDKLAARHGRSVNWHPILLGPIFKALGTNSLVNIPL-KGEYSKH 65 +Y SSP+ Y + L +H V P I G L + P + +Y Sbjct: 30 YYHALSSPWAYLGGPQFKALVEKHDLDVVVRPTR---IVTENGGVPLRSRPQPRQDYHAV 86 Query: 66 DMER-TARFHNIFYQAPSNFPIGSQVAARATLWVQQTQPAKAVDLIKTLYSAYFTEDIDI 124 +++R R P +P ++ +AR + Q A++L L A + E+ D+ Sbjct: 87 ELDRWRKRLGMPLVLKPRYYPTNNEFSARMVI-AADRQGLPALELSHALLRALWAEEQDV 145 Query: 125 SVTDHVLRIAADAGIDRHALEAALASPELKEQLRQRTDAAAKAGVFGSPFMIVDGEAFWG 184 + D + +A G+D AL A +PE+ A GVFG+P + +WG Sbjct: 146 TDPDVRIAVANSLGLDGRALRALEDTPEIMAAWHDSHAEAQSRGVFGTPTWVYRDTLYWG 205 Query: 185 FDRFTQLEAFL 195 DR T L+ L Sbjct: 206 QDRLTFLDDAL 216 >AGc3262 [Q] COG2761 Predicted dithiol-disulfide isomerase involved in polyketide biosynthesis Atu Agrobacterium tumefaciens strain C58 (Cereon) Length = 242 Score = 51.2 bits (121), Expect = 1e-06, Method: Compositional matrix adjust. Identities = 51/188 (27%), Positives = 75/188 (39%), Gaps = 18/188 (9%) Query: 5 IDFYFDFSSPYGYFAAVEID----KLAARHGRSVNWHPILLGPIFKALGTNSLVNIPLKG 60 +D D P+ Y +D ++ VNW P L P + G + L+ Sbjct: 26 VDVVSDMVCPWCYLGKARLDLAIAEVQDEISVDVNWRPYRLNPDYPPEGVDQ--KAALEE 83 Query: 61 EYSKHDMERT-ARFHNIFYQAPSNF-----PIGSQV--AARATLWVQQTQPAKAVDLIKT 112 + K +E+ A + Q N+ IG A R +LW ++ Sbjct: 84 KLGKDRLEQAHASLVELGKQVGINYDFDAIKIGPNTLDAHRLSLWAHAEGRDVQERIVTA 143 Query: 113 LYSAYFTEDIDISVTDHVL--RIAADAGIDRHALEAALASPELKEQLRQRTDAAAKAGVF 170 L+ A F E +I DH + IA AG+D + LAS K+ + DAA + GV Sbjct: 144 LFKANFEEGRNIG--DHAVLTDIAGKAGMDAKVVARLLASDADKDTVIAEIDAAQQMGVS 201 Query: 171 GSPFMIVD 178 G PF IVD Sbjct: 202 GVPFFIVD 209