BLASTP 2.2.21 [Jun-14-2009] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0001.4497.p (269 letters) Database: COGmodified 192,987 sequences; 59,019,183 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value SMb20214 [R] COG0300 Short-chain dehydrogenases of various subst... 346 2e-95 mll6686 [R] COG0300 Short-chain dehydrogenases of various substr... 318 7e-87 PA4832 [R] COG0300 Short-chain dehydrogenases of various substra... 253 4e-67 mll2507 [R] COG0300 Short-chain dehydrogenases of various substr... 248 1e-65 AGpA651 [R] COG0300 Short-chain dehydrogenases of various substr... 224 1e-58 >SMb20214 [R] COG0300 Short-chain dehydrogenases of various substrate specificities Sme Sinorhizobium meliloti Length = 267 Score = 346 bits (888), Expect = 2e-95, Method: Compositional matrix adjust. Identities = 173/257 (67%), Positives = 207/257 (80%) Query: 5 TTTQRGTALITGASSGIGAVYADRLARQGYDLILVARNRERLNALAERISNDTRRTVEVL 64 T +GTAL+TGASSGIGA+YADRLAR+GYDLILVARN+ RL+ LA R++++T R+VEV+ Sbjct: 2 TQHSKGTALVTGASSGIGAIYADRLARRGYDLILVARNQTRLDQLARRLTDETGRSVEVV 61 Query: 65 SADLGNAQSLAQVEDKLRRDASITLLVNNAGIGTHTPLLQSDVEHMTDMIALNVTAPTRL 124 +ADLG A++E LR DASIT+LVNNAG+G PLL SDV+ M +MIALNV A TRL Sbjct: 62 AADLGKRTDAARIEKVLRTDASITMLVNNAGVGATGPLLASDVDKMEEMIALNVNALTRL 121 Query: 125 TYAAVPGFVARGRGAIINISSIVGVAPEILNGVYGGSKAFVLAFSQSLHHELADQGVQVQ 184 TYAAVPGFVARG GAIINI+SIVG+APE+LNGVYGGSKAFVLAF+ SL EL + +++Q Sbjct: 122 TYAAVPGFVARGSGAIINIASIVGIAPEVLNGVYGGSKAFVLAFTLSLQKELEGRNIRIQ 181 Query: 185 AVLPGATATDFWAIGGLPLEHLDPAIVMPAEQMVDAALVGFERGELVTIPSLHDEDAWIR 244 AVLPGATATDFW I G P+EHL IVMPAE MVDAALVGF+ GE +TIPSL D W Sbjct: 182 AVLPGATATDFWGIAGTPVEHLPNEIVMPAEDMVDAALVGFDLGEPITIPSLPDAADWEA 241 Query: 245 FDSARQSLSTQLSANVP 261 +++ARQ L LS +VP Sbjct: 242 YEAARQKLVPNLSLSVP 258 >mll6686 [R] COG0300 Short-chain dehydrogenases of various substrate specificities Mlo Mesorhizobium loti Length = 276 Score = 318 bits (815), Expect = 7e-87, Method: Compositional matrix adjust. Identities = 161/258 (62%), Positives = 194/258 (75%) Query: 10 GTALITGASSGIGAVYADRLARQGYDLILVARNRERLNALAERISNDTRRTVEVLSADLG 69 GTA+ITGAS GIGAVY DRLA +GYDL+LVARN ++L +A R+ T R ++ LSADL Sbjct: 4 GTAVITGASGGIGAVYVDRLAERGYDLVLVARNGDKLTQVANRVRAKTGRKIDTLSADLA 63 Query: 70 NAQSLAQVEDKLRRDASITLLVNNAGIGTHTPLLQSDVEHMTDMIALNVTAPTRLTYAAV 129 NA LA+VE LR +TLLVNNAG+G LL SDV+ MT +I+LNVTA TRLTYA V Sbjct: 64 NASDLARVEAFLRETPDVTLLVNNAGLGGALKLLDSDVDQMTSLISLNVTALTRLTYAIV 123 Query: 130 PGFVARGRGAIINISSIVGVAPEILNGVYGGSKAFVLAFSQSLHHELADQGVQVQAVLPG 189 PGFVAR G IINI+SIV + PE LNGVYGGSKAFV+AFSQ+L HELA GV+VQ VLPG Sbjct: 124 PGFVARAAGTIINIASIVAINPESLNGVYGGSKAFVVAFSQNLRHELAGTGVRVQVVLPG 183 Query: 190 ATATDFWAIGGLPLEHLDPAIVMPAEQMVDAALVGFERGELVTIPSLHDEDAWIRFDSAR 249 ATATDFWAI G P+E L AIVM +E +VDAAL G +RGE TIPSL D + + +++AR Sbjct: 184 ATATDFWAIAGRPVEKLPKAIVMRSEDLVDAALAGLDRGEFATIPSLQDSELFDAYEAAR 243 Query: 250 QSLSTQLSANVPATRYAV 267 Q++ +LS PA RY + Sbjct: 244 QAMIGKLSTATPAPRYRI 261 >PA4832 [R] COG0300 Short-chain dehydrogenases of various substrate specificities Pae Pseudomonas aeruginosa Length = 265 Score = 253 bits (645), Expect = 4e-67, Method: Compositional matrix adjust. Identities = 136/256 (53%), Positives = 170/256 (66%) Query: 10 GTALITGASSGIGAVYADRLARQGYDLILVARNRERLNALAERISNDTRRTVEVLSADLG 69 G AL+TGASSGIGA YA+ LAR+G+DL+LVAR+R+RL ALA+R+ VE+L ADL Sbjct: 8 GVALVTGASSGIGATYAEHLARRGHDLLLVARDRQRLEALADRLRQAHGVRVEILRADLS 67 Query: 70 NAQSLAQVEDKLRRDASITLLVNNAGIGTHTPLLQSDVEHMTDMIALNVTAPTRLTYAAV 129 +VE +LR DASI LLVNNAG+ + PL +D++ MIALNV A TRL A Sbjct: 68 ERDDRLRVERRLRDDASIALLVNNAGVAMNGPLADADMDRAERMIALNVVALTRLAAGAA 127 Query: 130 PGFVARGRGAIINISSIVGVAPEILNGVYGGSKAFVLAFSQSLHHELADQGVQVQAVLPG 189 GF RG GAI+N+ S+V +APE+ N VY +KA+VL+ SQSL HELA GV VQAVLPG Sbjct: 128 EGFRRRGGGAIVNLGSVVALAPELFNAVYSATKAYVLSLSQSLQHELAGSGVYVQAVLPG 187 Query: 190 ATATDFWAIGGLPLEHLDPAIVMPAEQMVDAALVGFERGELVTIPSLHDEDAWIRFDSAR 249 T T+ W G + + +VM E +V+AALVGF+R E VTIPSL D W +AR Sbjct: 188 VTRTEIWERSGTGIAGIPAEMVMEVEDLVEAALVGFDRREAVTIPSLPDAADWQALMTAR 247 Query: 250 QSLSTQLSANVPATRY 265 L+ LS PA RY Sbjct: 248 ARLAPNLSRQRPAERY 263 >mll2507 [R] COG0300 Short-chain dehydrogenases of various substrate specificities Mlo Mesorhizobium loti Length = 264 Score = 248 bits (632), Expect = 1e-65, Method: Compositional matrix adjust. Identities = 131/259 (50%), Positives = 174/259 (67%), Gaps = 1/259 (0%) Query: 8 QRGTALITGASSGIGAVYADRLARQGYDLILVARNRERLNALAERISNDTRRTVEVLSAD 67 +GTA++TGASSGIGA+YADRLA QGYDL+LVAR +RL LAE++ R V V+SAD Sbjct: 6 NKGTAVVTGASSGIGAIYADRLAGQGYDLVLVARRADRLEELAEKLRYAYDRKVSVISAD 65 Query: 68 LGNAQSLAQVEDKLRRDASITLLVNNAGIGTHTPLLQSDVEHMTDMIALNVTAPTRLTYA 127 L + + +VE + D S+TLLVNNAG+G + +D + MI +NV A TRLT A Sbjct: 66 LSDDDDVRRVEQAISADDSVTLLVNNAGLGGQQVVATADADAAERMIKVNVIALTRLTRA 125 Query: 128 AVPGFVARGRGAIINISSIVGVAPEILNGVYGGSKAFVLAFSQSLHHELADQGVQVQAVL 187 +PG +AR RGAI+NI+S++ G+Y G+KA+V+ F+++LH E+A GV+VQ VL Sbjct: 126 VLPGLLARNRGAIVNIASVLAYETS-FGGIYSGTKAYVVNFTEALHREVAGTGVKVQVVL 184 Query: 188 PGATATDFWAIGGLPLEHLDPAIVMPAEQMVDAALVGFERGELVTIPSLHDEDAWIRFDS 247 PGAT TDFW + G ++ L I+M A+ MVDAALVG RGE VT+P+L D F Sbjct: 185 PGATRTDFWELAGSDIDQLPKEIIMSADDMVDAALVGLARGEAVTVPALADAAKLDTFLG 244 Query: 248 ARQSLSTQLSANVPATRYA 266 ARQ+ L A+ PA RYA Sbjct: 245 ARQAFYGSLHADKPAARYA 263 >AGpA651 [R] COG0300 Short-chain dehydrogenases of various substrate specificities Atu Agrobacterium tumefaciens strain C58 (Cereon) Length = 287 Score = 224 bits (572), Expect = 1e-58, Method: Compositional matrix adjust. Identities = 125/259 (48%), Positives = 159/259 (61%), Gaps = 2/259 (0%) Query: 7 TQRGTALITGASSGIGAVYADRLARQGYDLILVARNRERLNALAERISNDTRRTVEVLSA 66 +Q LITGAS+GIGA YA+R AR+G+DL+LVAR+ R+ ALA R+S +T +++L A Sbjct: 27 SQISAVLITGASTGIGATYAERFARRGHDLVLVARDMARMEALASRLSQETGVRIDILPA 86 Query: 67 DLGNAQSLAQVEDKLRRDASITLLVNNAGIGTHTPLLQSDVEHMTDMIALNVTAPTRLTY 126 DL A L +VE +LR D I +LVNNAG + V+ MT ++ALN TA RL Sbjct: 87 DLTQATDLVKVETRLRDDERIGILVNNAGTAIGGTFVDQSVDDMTRLVALNATALVRLAG 146 Query: 127 AAVPGFVARGRGAIINISSIVGVAPEILNGVYGGSKAFVLAFSQSLHHELADQGVQVQAV 186 A P G GAI+NI S+VG+APE VYG +KAFVL SQ L HEL +GV VQAV Sbjct: 147 AIAPRLAKAGEGAIVNIGSVVGLAPEFGMTVYGATKAFVLFLSQGLAHELGPKGVYVQAV 206 Query: 187 LPGATATDFWAIGGLPLEHLDPAIVMPAEQMVDAALVGFERGELVTIPSLHDEDAWIRFD 246 LP T T+ W G + L+ VM +VDAALVGF+R E VTIP L D W FD Sbjct: 207 LPATTRTEIWDHVGADVNTLNN--VMEVGDLVDAALVGFDRREPVTIPPLPDAGQWEAFD 264 Query: 247 SARQSLSTQLSANVPATRY 265 AR+++ +P RY Sbjct: 265 LARKTMMANFGNALPGERY 283