BLASTP 2.2.21 [Jun-14-2009] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0001.4468.p (272 letters) Database: COGmodified 192,987 sequences; 59,019,183 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value RSp0233 [R] COG3618 Predicted metal-dependent hydrolase of the T... 268 1e-71 Cj0556 [R] COG3618 Predicted metal-dependent hydrolase of the TI... 184 2e-46 YPO1933 [R] COG3618 Predicted metal-dependent hydrolase of the T... 150 2e-36 AGl3341 [R] COG3618 Predicted metal-dependent hydrolase of the T... 66 1e-10 Cgl0451 Non COG 62 1e-09 >RSp0233 [R] COG3618 Predicted metal-dependent hydrolase of the TIM-barrel fold Rso Ralstonia solanacearum Length = 302 Score = 268 bits (685), Expect = 1e-71, Method: Compositional matrix adjust. Identities = 146/266 (54%), Positives = 183/266 (68%), Gaps = 2/266 (0%) Query: 8 IDTHAHVFARGLPMADGRRYTPAYDVTVEQYLARLDAHGMSHGVLVQPSFLGSDNSYLLQ 67 IDTHAHVF RGLP+A RRY P+YD T+ YLA+LDAHG+SHGVL+QPSFLG DNSYLL Sbjct: 38 IDTHAHVFKRGLPLAGARRYAPSYDATLPAYLAQLDAHGLSHGVLIQPSFLGGDNSYLLA 97 Query: 68 AIARAPQRLRGIAMVDADISDTELQALQQGGVVGVRFNLVGGAALPDFKG-AWRDTLTRI 126 A+ +APQRLRG+A++D + L L G+VG+R NL+G A P + AW+ L R+ Sbjct: 98 ALRQAPQRLRGVAVIDPAAPEPFLAQLNAEGIVGIRLNLIG-APDPQLRAPAWQAALHRL 156 Query: 127 AALDWQVEIHREAIDLPLVLDPLLERGMKVVVDHFGRVDAGLGVADPGFRYLLSTGASRH 186 AL W VE+H +A LP +L PLLE + VVVDHFGR D L V DPGF LL+ G +R Sbjct: 157 HALGWHVELHTQAHRLPALLPPLLEAQVDVVVDHFGRPDPALSVEDPGFAALLAAGRTRR 216 Query: 187 VWVKLSAQYRNGGAQGGAGFARQAWPLLREHLGTDRLLWGSDWPHTQHESVTDYADSWLR 246 VWVK+S YRNG A G A A P L++ LGTDRL+WGSDWPHTQ+ES Y + Sbjct: 217 VWVKVSGAYRNGDAGRGEAIALAAMPRLKDALGTDRLVWGSDWPHTQYESRIGYDSARAF 276 Query: 247 FEQLVSDEQDRHRIIGQTAAQLFHIA 272 + L+ D DRH+++ +T A+LF A Sbjct: 277 ADTLLPDAGDRHQVLVETPARLFRFA 302 >Cj0556 [R] COG3618 Predicted metal-dependent hydrolase of the TIM-barrel fold Cje Campylobacter jejuni Length = 265 Score = 184 bits (466), Expect = 2e-46, Method: Compositional matrix adjust. Identities = 94/271 (34%), Positives = 152/271 (56%), Gaps = 15/271 (5%) Query: 6 LRIDTHAHVFARGLPMADGRRYTPAYDVTVEQYLARLDAHGMSHGVLVQPSFLGSDNSYL 65 ++IDTHAH+F + L RY P YD + + Y A LD + + GVLVQPSFLG DN +L Sbjct: 1 MKIDTHAHIFLKKLNTVANARYKPDYDASFKDYKANLDHYDFNKGVLVQPSFLGIDNEFL 60 Query: 66 LQAIARAPQRLRGIAMVDADISDTELQALQQGGVVGVRFNLVGGAALPDFKG-AWRDTLT 124 LQ+I + + ++ I +VD +I EL+ L++ GVR NL+ G LP+FK W Sbjct: 61 LQSIEK-DENIKAIVVVDENIKFDELKKLKERKACGVRLNLI-GKELPNFKSMVWTQFFE 118 Query: 125 RIAALDWQVEIHREAIDLPLV--LDPLLERGMKVVVDHFGRVDAGLGVADPGFRYLLSTG 182 ++ L Q+EI R+ +D LV ++ L+ G +V+DH R +A L L+ Sbjct: 119 NLSKLKMQIEIQRD-LDNNLVDIVENLIPYGCNIVIDHLARANANL----TNLEDLICLK 173 Query: 183 ASRHVWVKLSAQYR----NGGAQGGAGFARQAWPLLREHLGTDRLLWGSDWPHTQHESVT 238 SR ++ K+S YR + FA++ + +L+EH ++GSDWPHT E+ Sbjct: 174 NSR-IFFKISGFYRAKIDYANNEQAVKFAKKIYEILKEHFPLSNFVFGSDWPHTNFEANI 232 Query: 239 DYADSWLRFEQLVSDEQDRHRIIGQTAAQLF 269 +++ + + F ++V ++++ +I+G A LF Sbjct: 233 NFSSALVAFNEVVVSKKEQEQILGDNACALF 263 >YPO1933 [R] COG3618 Predicted metal-dependent hydrolase of the TIM-barrel fold Ype Yersinia pestis Length = 289 Score = 150 bits (380), Expect = 2e-36, Method: Compositional matrix adjust. Identities = 98/269 (36%), Positives = 139/269 (51%), Gaps = 15/269 (5%) Query: 8 IDTHAHVFA-RGL-PMADGRRYTPAYDVTVEQYLARLDAHGMSHGVLVQPSFLGSDNSYL 65 D+HAHV + R + PM R YTP E+YL+ L A GMS GVLVQ S GSDN Y+ Sbjct: 27 CDSHAHVISPRAIYPMVAERSYTPP-PAPEEKYLSMLAATGMSRGVLVQISVYGSDNRYM 85 Query: 66 LQAIARAPQRLRGIAMVDADISDTELQALQQGGVVGVRFNLVGGAALPDFKGAWRDTLTR 125 LQ + R P+ LRG+A+V D++D ELQ + GV G+R N++ G + F A + ++ Sbjct: 86 LQVLKRHPEYLRGVAVVTEDVTDQELQDMALAGVRGLRINVLFGGGI-GFD-AMENLASK 143 Query: 126 IAALDWQVEIHREAIDLPLVLDPLLERGMKVVVDHFGRVDAGLGVADPGFRYLLSTGASR 185 IA L W ++ + LP ++ + + V+DH G + LG+ PG++ LL Sbjct: 144 IAPLGWHMQFLMDVRQLPALIPRMKKLPCPCVIDHMGHMPVSLGLNHPGYQTLLHMVREY 203 Query: 186 HVWVKLSAQYR-----NGGAQGGAGFARQAWPLLREHLGTDRLLWGSDWPHTQHESVTDY 240 WVKLS YR G FA++ DR++WGSDWPH + D Sbjct: 204 GWWVKLSGAYRISESWQDGYLDVVPFAQKLI-----ETAPDRMVWGSDWPHVSVSRMPDT 258 Query: 241 ADSWLRFEQLVSDEQDRHRIIGQTAAQLF 269 V DEQ R+RI+ A+L+ Sbjct: 259 GGLRNLLAAWVPDEQVRNRILVDNPARLY 287 >AGl3341 [R] COG3618 Predicted metal-dependent hydrolase of the TIM-barrel fold Atu Agrobacterium tumefaciens strain C58 (Cereon) Length = 292 Score = 65.9 bits (159), Expect = 1e-10, Method: Compositional matrix adjust. Identities = 60/252 (23%), Positives = 103/252 (40%), Gaps = 30/252 (11%) Query: 36 EQYLARLDAHGMSHGVLVQPSFLGSDNSYLLQAIARAPQRLRGIAMVDADISDTELQALQ 95 E Y + G+ ++ Q + DN L +A + + ++DA ++ +++ L Sbjct: 51 EDYRRLMQWLGIDRVIITQGNAHQRDNGNTLACVAEMGEAAHAVVIIDATTTEKDMEKLT 110 Query: 96 QGGVVGVR-FNLVGGAA-LPDFKGAWRDTLTRIAALDWQVEIHREAIDLPLVLDPLLERG 153 G VG R +L GGA L + R A DW V + + L L L + Sbjct: 111 AAGTVGARIMDLPGGAVNLSELDA----VDERAHAADWMVAVQFDGNGLLDHLPRLQKIR 166 Query: 154 MKVVVDHFGRVDAGLGVADPGFRYLLSTGASRHVWVKLSAQYRNGGAQGGAGFARQAWP- 212 + V DH G+ G+ P LL ++W K + Y + +R++WP Sbjct: 167 SRWVFDHHGKFFKGIRTDGPEMAALLKLIDRGNLWFKFAGVYES---------SRKSWPY 217 Query: 213 --------LLREHLGTDRLLWGSDWPHTQHESVTDYADSWLRFEQL----VSDEQDRHRI 260 ++ H +R++WG++WPH Y D R +L + DE RHR Sbjct: 218 ADVAAFSRVIAAH-APERIVWGTNWPHNSVRETAAYPDD-ARLAELTLGWLPDEAARHRA 275 Query: 261 IGQTAAQLFHIA 272 + + LF ++ Sbjct: 276 LVENPEALFKLS 287 >Cgl0451 Non COG Length = 250 Score = 62.4 bits (150), Expect = 1e-09, Method: Compositional matrix adjust. Identities = 73/258 (28%), Positives = 108/258 (41%), Gaps = 34/258 (13%) Query: 8 IDTHAHVFARGLPMADGRRYTPAYDVTVEQYLARLDAHGMSHGVLVQPSFLGSDNSYLLQ 67 D+H H+ P+ + Y P TVE Y AR++ ++ G +V SF D YL Sbjct: 5 FDSHFHIIDPQHPLIENNGYLPE-PFTVEDYTARVEGLEVAGGAIVSGSFQAFDQGYLKD 63 Query: 68 AIARAPQRLRGIAMVDADISDTELQALQQGGVVGVRFNLV--GGAALPDFKGAWRDTLTR 125 A+A G+ + AD SD E+ L + GV VR NL G A L D + R R Sbjct: 64 ALAVLGPGYVGVTQIPADTSDQEILDLDKAGVKAVRLNLKRGGSAGLDDLETLAR----R 119 Query: 126 IAAL-DWQVEIHREAIDLPLVLDPLLERGMKVVVDHFGRVDAGLGVADPGFRYLLSTGAS 184 + L W E++ +A +L L+ L V +DH G GL P L+ G Sbjct: 120 VHDLAGWHTELYVDARELD-ELESTLASLPAVSIDHLGLHRDGL----PALLRLVENG-- 172 Query: 185 RHVWVKLSAQYRNGGAQGGAGFAR---QAWPLLREHLGTD--RLLWGSDWPHTQHESVTD 239 +K+ A GF R +++ + D L+ G+D P T+ + + Sbjct: 173 ----IKVKA----------TGFGRVELDPTEVIQAIMAVDPTALMIGTDLPSTRTKRPFE 218 Query: 240 YADSWLRFEQLVSDEQDR 257 AD L E + D D Sbjct: 219 DADLDLIAETVGEDHVDN 236