BLASTP 2.2.21 [Jun-14-2009] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0001.4380.p (299 letters) Database: COGmodified 192,987 sequences; 59,019,183 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value AGc5105 [R] COG3618 Predicted metal-dependent hydrolase of the T... 135 1e-31 mll5767 Non COG 86 7e-17 SMb21101 [R] COG3618 Predicted metal-dependent hydrolase of the ... 72 2e-12 mlr1708 Non COG 70 4e-12 BMEI1718 [R] COG3618 Predicted metal-dependent hydrolase of the ... 69 1e-11 >AGc5105 [R] COG3618 Predicted metal-dependent hydrolase of the TIM-barrel fold Atu Agrobacterium tumefaciens strain C58 (Cereon) Length = 287 Score = 135 bits (340), Expect = 1e-31, Method: Compositional matrix adjust. Identities = 90/284 (31%), Positives = 134/284 (47%), Gaps = 17/284 (5%) Query: 15 IDAHQHFWRYRSEDYPWIGTGMDLLAQDRLPYQFQPLLEAQGLHASITVQARPGREETAF 74 IDAHQHFW + W + + +D LP PLL A G+ ++ VQ +T F Sbjct: 17 IDAHQHFWLMKDRAGQWPPPSLAAIYRDFLPTDLSPLLTAAGVSGTVLVQTMETAADTDF 76 Query: 75 LLEMARRDARIVGVIGWDDISSPQLADCMEQWGRHKLL-GLRHAMPETDDATLLLAQPQF 133 +LE+A I GV+GW D+ S ++ + + +H G+R + +D + Sbjct: 77 MLELAENTDFIKGVVGWVDMKSAEVTAEIARRAKHPAFKGVRPMLQGMEDVA-WIDDAAL 135 Query: 134 NHGVRWLQERDYVLDVLAHERHLPQLRQLCALHDQHWIVLNHLGKPALKEFRKGRAAYER 193 N V L + V D L +LP L + H + +V++H KP + Y Sbjct: 136 NAAVEALVKHGLVFDALVLPPNLPHLLSFASKHPELPVVIDHGAKPLI-----ATGHYRD 190 Query: 194 WRRELRELTAMPHVACKISGLLTEADWLRGLCSRDFDHIVQCLDTALELFGPQRLMFGSD 253 WRR + L A P+V CK+SGLLTE R R + +T LELFG R++FGSD Sbjct: 191 WRRNMAALAARPNVHCKLSGLLTERGEQRPEAVRPY------AETILELFGGDRVIFGSD 244 Query: 254 WPVCLLAASYARVVAVVREWAAKRLCATDQDALWGESSANIYGL 297 WPV LA + + R+ + A D A++G ++ Y L Sbjct: 245 WPVLRLAGDFQGWLDFCRDI----VPAKDHAAVFGGNAIRFYSL 284 >mll5767 Non COG Length = 297 Score = 86.3 bits (212), Expect = 7e-17, Method: Compositional matrix adjust. Identities = 78/303 (25%), Positives = 129/303 (42%), Gaps = 32/303 (10%) Query: 15 IDAHQHFWRYRSEDYPWIGTGMDLLAQD-----RLPYQFQPLLE-------AQGLHASIT 62 ID H H W YPW+ G+ A R Y+ LE + +H + Sbjct: 7 IDPHHHLWDLEVNYYPWLTDGVKPSAFGDYEAIRTNYRLDDYLEDARNQNLVKSVHLDVG 66 Query: 63 VQARPGREETAFLLEMARRDARIVGVIGWDDISSPQLADCMEQWGRH-KLLGLRHAMP-E 120 ET +L E+A + G++G+ D+S P + + +++ + G+R +M Sbjct: 67 FDPSDPVGETRWLQEIANKRGFPHGIVGYADLSKPDVGELLDRHMEYPNFRGIRQSMNYH 126 Query: 121 TDDATLLLAQPQFNH------GVRWLQERDYVLDVLAHERHLPQLRQLCALHDQHWIVLN 174 D A A+P+ + G R L +R D+ + + + QL I+LN Sbjct: 127 ADPAKTYQARPEVSRTPEWRRGFRELAKRRLSFDLQLYYPQMEEFLQLAHDFPDVQIILN 186 Query: 175 HLGKPALKEFRKGRAAYERWRRELRELTAMPHVACKISGLLTEADWLRGLCSRDFDHIVQ 234 H G G + W++ + L P+VACKISG L DW S I Sbjct: 187 HTGMQV-----DGPKHFNAWKKGMHRLAQAPNVACKISG-LGMGDWSWTTAS-----IRP 235 Query: 235 CLDTALELFGPQRLMFGSDWPVCLLAASYARVVAVVREWAAKRLCATDQDALWGESSANI 294 ++ A+ FG R MF +++PV L +S+ VV +E + ++ AL+ +++A Sbjct: 236 YVEEAIAAFGVGRSMFATNFPVDKLFSSFDAVVNAFKE-ITRYYPHEERLALFHDNAARH 294 Query: 295 YGL 297 Y L Sbjct: 295 YRL 297 >SMb21101 [R] COG3618 Predicted metal-dependent hydrolase of the TIM-barrel fold Sme Sinorhizobium meliloti Length = 278 Score = 71.6 bits (174), Expect = 2e-12, Method: Compositional matrix adjust. Identities = 73/272 (26%), Positives = 119/272 (43%), Gaps = 37/272 (13%) Query: 15 IDAHQHFWRYRSEDYPWIGTGMDLLAQDRLPYQFQPLLEAQGLHASITVQARPGREETAF 74 ID H H + +YPW+ G+ L +D L + + G+ AS+ ++ E Sbjct: 3 IDTHLHLIDKSALNYPWLA-GVPALDRDFLYATYAAEAKRVGVAASLHMEVDVDPAE--- 58 Query: 75 LLEMARRD-ARIVGVIGWDDISSPQLADC----------MEQWGRHKLL-GLRHAM-PET 121 +E+ R+ AR+ G G + +A C +E+ + + G R + T Sbjct: 59 -IELETREVARLAGEPG--SLLKGAIAACRPEDEGFAAYLERQEENAFVKGFRRVLHVVT 115 Query: 122 DDATLLLAQPQFNHGVRWLQERDYVLDVLAHERHLPQLRQLCALHDQHWIVLNHLGKPAL 181 DD L QP F V+ L + D+ +P+ L L +L+H G P + Sbjct: 116 DD---LSEQPLFRENVKRLSGTRFTFDLCVLPHQIPKAIALADLAPDVQFILDHCGVPDI 172 Query: 182 KEFRKGRAAYERWRRELRELTAMPHVACKISGLLT--EADWLRGLCSRDFDHIVQCLDTA 239 K G A + WR + E+ P+V KISG++ E DW D I ++ Sbjct: 173 K----GHAEHP-WRDHMTEIARHPNVVAKISGVVAYAEEDWA-------LDSIRPYVEHT 220 Query: 240 LELFGPQRLMFGSDWPVCLLAASYARVVAVVR 271 + +FG R+++GSDWPVC L + + VA + Sbjct: 221 ISVFGWDRVVWGSDWPVCTLGGNLSTWVAATQ 252 >mlr1708 Non COG Length = 295 Score = 70.5 bits (171), Expect = 4e-12, Method: Compositional matrix adjust. Identities = 71/298 (23%), Positives = 125/298 (41%), Gaps = 32/298 (10%) Query: 13 RRIDAHQHFWRYRSEDYPWIGTGM--------DLLAQDRLPYQFQPLLEAQGLHASITVQ 64 + IDAH H WR +D PW+ M + + +D +F + G+ ++ VQ Sbjct: 3 KAIDAHFHIWR--QKDQPWLVGPMVPRIFGPYEPIRRDYPIGEFLQDQKGSGVEKAVYVQ 60 Query: 65 ARPGRE----ETAFLLEMARRDARIVGVIGWDDISSPQLADCMEQWGRHKLL-GLR---- 115 +E E AFL + A ++G+ D++ + +++ +++LL G+R Sbjct: 61 TNWAKEDFEKEVAFLQQTADETGWPHAIVGYADMTVDDVRPQIDRLMKYRLLRGVRMQLH 120 Query: 116 -HAMPETDDATLL--LAQPQFNHGVRWLQERDYVLDVLAHERHLPQLRQLCALHDQHWIV 172 H P A + P+ V L++ D+ + L + + + Sbjct: 121 WHETPAFRFAASADQVIDPKVRANVARLKDYGLSFDLQLFPAQMKDGVTLVGENPETNFI 180 Query: 173 LNHLGKPALKEFRKGRAAYERWRRELRELTAMPHVACKISGLLTEADWLRGLCSRDFDHI 232 L H G E E W+ LR L+A P+ K+SGL T + D I Sbjct: 181 LTHAGMLTGME----PEVTETWKAGLRTLSAAPNFYAKLSGLGT------FVHRNDPALI 230 Query: 233 VQCLDTALELFGPQRLMFGSDWPVCLLAASYARVVAVVREWAAKRLCATDQDALWGES 290 +D A+++ G LMFGS++P+ L S+A ++ R+ A+ A + D W + Sbjct: 231 AYIVDNAIDILGADHLMFGSNFPIEKLWTSHAELIKAHRDAVARHGAAAEADIFWNTA 288 >BMEI1718 [R] COG3618 Predicted metal-dependent hydrolase of the TIM-barrel fold Bme Brucella melitensis Length = 282 Score = 69.3 bits (168), Expect = 1e-11, Method: Compositional matrix adjust. Identities = 67/257 (26%), Positives = 108/257 (42%), Gaps = 24/257 (9%) Query: 15 IDAHQHFWRYRSEDYPWIGTGMDLLAQDRLPYQFQPLLEAQG----LHASITVQARPGRE 70 ID H H + YPW+ + L +D L ++ E G LH + V + Sbjct: 3 IDTHLHLIDKNALSYPWLESA-PALNRDFLFDSYRLQAERAGIGGALHIEVDVAPHAMQA 61 Query: 71 ETAFLLEMARRDARIV-GVIGWDDISSPQLADCME-QWGRHKLLGLR---HAMPETDDAT 125 ET + ++ARR + G I P A +E Q + GLR H +P+ Sbjct: 62 ETDHIRQIARRSGGFIKGAIASCRPEEPGFAAYLERQLSDPFVKGLRRVLHVVPDEVSEG 121 Query: 126 LLLAQPQFNHGVRWLQERDYVLDVLAHERHLPQLRQLCALHDQHWIVLNHLGKPALKEFR 185 L F ++ L+ D+ + + L L +++H G P +K Sbjct: 122 AL-----FRENIKRLEGTGLTFDLCVQPHQIGKAIALADLAPDLCFIVDHCGAPDIKA-- 174 Query: 186 KGRAAYERWRRELRELTAMPHVACKISGLLTEADWLRGLCSRDFDHIVQCLDTALELFGP 245 A + WR + E++ P+V KISGL+ D S + + + ++ FG Sbjct: 175 ---GALDGWREGMAEISRRPNVIAKISGLVVYCD----AESWTVETLRPYAEQVIQSFGW 227 Query: 246 QRLMFGSDWPVCLLAAS 262 R+++GSDWPVC LA+S Sbjct: 228 DRVIWGSDWPVCTLASS 244