BLASTP 2.2.21 [Jun-14-2009] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0001.4353.p (252 letters) Database: COGmodified 192,987 sequences; 59,019,183 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value RSc1822 [Q] COG3473 Maleate cis-trans isomerase Rso Ralstonia so... 468 e-132 PH0985 [Q] COG3473 Maleate cis-trans isomerase Pho Pyrococcus ho... 134 2e-31 PAB2420 [Q] COG3473 Maleate cis-trans isomerase Pab Pyrococcus a... 132 6e-31 PH1150 [Q] COG3473 Maleate cis-trans isomerase Pho Pyrococcus ho... 129 6e-30 APE2520 [Q] COG3473 Maleate cis-trans isomerase Ape Aeropyrum pe... 124 2e-28 >RSc1822 [Q] COG3473 Maleate cis-trans isomerase Rso Ralstonia solanacearum Length = 252 Score = 468 bits (1203), Expect = e-132, Method: Compositional matrix adjust. Identities = 230/252 (91%), Positives = 237/252 (94%) Query: 1 MSSTRSYRIGQIVPSSNTTMETEIPAILRARELNLPERFTFHSSRMRMKHVTKEELAAMD 60 MS+ R+YRIGQIVPSSNTTMETEIPAILRAREL PERFTFHSSRMRMKHVTKEELAAMD Sbjct: 1 MSTARAYRIGQIVPSSNTTMETEIPAILRARELTQPERFTFHSSRMRMKHVTKEELAAMD 60 Query: 61 GDSDRCALELSDARVDVLGYACLVAIMSMGRGYHRVSEQRLHQRTVDNGGAAPVVTSAGA 120 DSDRCALELSDARVDVLGYACLVAIMSMG GYHRVS+ RLHQRT +NGG APVVTSAGA Sbjct: 61 ADSDRCALELSDARVDVLGYACLVAIMSMGLGYHRVSQARLHQRTQENGGPAPVVTSAGA 120 Query: 121 LVDGLHAIGARKVSILTPYMKPLTQLVIDYIENEGIEVVDSISLEIPDNLEVGRQDPRAP 180 LVDGL AIGARKVS+L PYMKPLTQLVIDYIENEGIEVVDSISLEIPDNLEVGRQDPRAP Sbjct: 121 LVDGLKAIGARKVSLLAPYMKPLTQLVIDYIENEGIEVVDSISLEIPDNLEVGRQDPRAP 180 Query: 181 IEITKRLNTSGVDAIVASACVQMPSLASVQAIEDRTGLPVLSSSVATTYMMLKRLELDTT 240 IEITKRL T+GVDAIVASACVQMPSL SVQAIEDRTGLPVLSSSVATTY+MLKRL LDT Sbjct: 181 IEITKRLKTTGVDAIVASACVQMPSLPSVQAIEDRTGLPVLSSSVATTYLMLKRLGLDTK 240 Query: 241 VPGFGSLLSGRY 252 VPGFGSLLSG Y Sbjct: 241 VPGFGSLLSGAY 252 >PH0985 [Q] COG3473 Maleate cis-trans isomerase Pho Pyrococcus horikoshii Length = 238 Score = 134 bits (336), Expect = 2e-31, Method: Compositional matrix adjust. Identities = 77/244 (31%), Positives = 131/244 (53%), Gaps = 13/244 (5%) Query: 8 RIGQIVPSSNTTMETEIPAILRARELNLPERFTFHSSRMRMKHVTKEELAAMDGDSDRCA 67 RIG IVPSSNTTME E + PE + H++RMR++ VT+E L M+ + A Sbjct: 7 RIGLIVPSSNTTMEPEFWKMA-------PEGVSIHTARMRLREVTEESLLEMERHAKDAA 59 Query: 68 LELSDARVDVLGYACLVAIMSMGRGYHRVSEQRLHQRTVDNGGAAPVVTSAGALVDGLHA 127 LEL+DA VDV+ Y C + G+GY + + L + +T++ A+++ L+ Sbjct: 60 LELADAEVDVIVYGCTSGSLIKGKGYDKEIAKNLEE-----ASGIKTITTSTAVLNALNT 114 Query: 128 IGARKVSILTPYMKPLTQLVIDYIENEGIEVVDSISLEIPDNLEVGRQDPRAPIEITKRL 187 + +KV + TPY+ + + +++E GIEV+ L I N E+GRQ+P ++ + Sbjct: 115 LDIQKVVVATPYIDSVNEREKEFLEANGIEVLRIKGLGIVKNTEIGRQEPWVAYKLALEV 174 Query: 188 NTSGVDAIVASACVQMPSLASVQAIEDRTGLPVLSSSVATTYMMLKRLELDTTVPGFGSL 247 T D + S C ++ + +E G+PV++S+ AT + LK +++ +G+L Sbjct: 175 YTPEADGLFIS-CTNFRTIEIIDKLEVELGVPVVTSNQATMWYALKTMKVKEKYDMYGTL 233 Query: 248 LSGR 251 L + Sbjct: 234 LKEK 237 >PAB2420 [Q] COG3473 Maleate cis-trans isomerase Pab Pyrococcus abyssi Length = 236 Score = 132 bits (333), Expect = 6e-31, Method: Compositional matrix adjust. Identities = 78/241 (32%), Positives = 130/241 (53%), Gaps = 14/241 (5%) Query: 8 RIGQIVPSSNTTMETEIPAILRARELNLPERFTFHSSRMRMKHVTKEELAAMDGDSDRCA 67 RIG IVPSSNTTME E ++L PE + H+SRM +K VT+EEL M + A Sbjct: 7 RIGLIVPSSNTTMEMEFHSVL-------PEGVSLHTSRMPLKSVTEEELLNMSSYAIEAA 59 Query: 68 LELSDARVDVLGYACLVAIMSMGRGYHRVSEQRLHQRTVDNGGAAPVVTSAGALVDGLHA 127 L+DA V+V+ + C G+ + + E ++ + T++ A+++ L+ Sbjct: 60 KLLADAGVEVIAFGCTSGSFIGGKDFEKELEMKIEKEV-----NVETFTTSTAVIEALNI 114 Query: 128 IGARKVSILTPYMKPLTQLVIDYIENEGIEVVDSISLEIPDNLEVGRQDPRAPIEITKRL 187 + + V ++TPY+ + Q +++E G EVVD L I DN+E+G+ +P + K Sbjct: 115 LEMQTVQVITPYIDEINQREKEFLEANGFEVVDIRGLGISDNIEIGKLEPYIAYRLAKAS 174 Query: 188 NTSGVDAIVASACVQMPSLASVQAIEDRTGLPVLSSSVATTYMMLKRLELDTTVPGFGSL 247 T VD I S C + ++ +E G+PV++S+ AT +M L+++++ +P G L Sbjct: 175 FTGDVDGIFIS-CTNFRTFEIIEKLERDLGVPVVTSNQATLWMALRQIDVKDKLP-LGKL 232 Query: 248 L 248 L Sbjct: 233 L 233 >PH1150 [Q] COG3473 Maleate cis-trans isomerase Pho Pyrococcus horikoshii Length = 236 Score = 129 bits (324), Expect = 6e-30, Method: Compositional matrix adjust. Identities = 76/241 (31%), Positives = 130/241 (53%), Gaps = 14/241 (5%) Query: 8 RIGQIVPSSNTTMETEIPAILRARELNLPERFTFHSSRMRMKHVTKEELAAMDGDSDRCA 67 RIG IVPSSNTTME E+ + L PE + H+SRM +K +T EEL M+ + A Sbjct: 7 RIGLIVPSSNTTMEMELHSFL-------PEGVSLHTSRMPLKSITDEELFKMNSYAIEAA 59 Query: 68 LELSDARVDVLGYACLVAIMSMGRGYHRVSEQRLHQRTVDNGGAAPVVTSAGALVDGLHA 127 L+DA V+V+ + C G+ + + E ++ + T++ A+++ L Sbjct: 60 SLLADAGVEVIAFGCSSGSFIGGQDFEKELEMKIEEEV-----NIETFTTSTAVLEALDV 114 Query: 128 IGARKVSILTPYMKPLTQLVIDYIENEGIEVVDSISLEIPDNLEVGRQDPRAPIEITKRL 187 + A+ + I+TPY + Q +++E G EV+D L + DNLE+G+ +P + K + Sbjct: 115 LDAQSLLIVTPYSDEINQKEKEFLEGNGFEVLDIKGLNLTDNLEIGKLEPYVVYRLAKAM 174 Query: 188 NTSGVDAIVASACVQMPSLASVQAIEDRTGLPVLSSSVATTYMMLKRLELDTTVPGFGSL 247 DAI S C + + ++ +E G+PV++S+ AT +M L+++++ +P G L Sbjct: 175 FAGDADAIFIS-CTNLRTFEIIEKLERDLGVPVITSNQATLWMALRQIDVRDQLP-LGKL 232 Query: 248 L 248 L Sbjct: 233 L 233 >APE2520 [Q] COG3473 Maleate cis-trans isomerase Ape Aeropyrum pernix Length = 272 Score = 124 bits (312), Expect = 2e-28, Method: Compositional matrix adjust. Identities = 78/228 (34%), Positives = 127/228 (55%), Gaps = 13/228 (5%) Query: 8 RIGQIVPSSNTTMETEIPAILRARELNLPERFTFHSSRMRMKHVTKEELAAMDGDSDRCA 67 RIG IVPSSNTTME E+ + PE + H+ R+ ++ V+ EEL M+ S A Sbjct: 42 RIGLIVPSSNTTMEPEM-------WMMAPEGVSIHTGRVPLEKVSVEELLKMEEYSAIEA 94 Query: 68 LELSDARVDVLGYACLVAIMSMGRGYHRVSEQRLHQRTVDNGGAAPVVTSAGALVDGLHA 127 +L+ A VD++ Y C + G GY +L + VVT+A A+V+ L A Sbjct: 95 EKLATAGVDLIVYGCTTGSLIGGPGYDLKIADKL-----SSASGVQVVTTATAVVEALKA 149 Query: 128 IGARKVSILTPYMKPLTQLVIDYIENEGIEVVDSISLEIPDNLEVGRQDPRAPIEITKRL 187 +GA ++++ TPY++ + + +++ + G ++VD SL + NL++GR P + + L Sbjct: 150 LGASRIAVATPYIEEVNKREEEFLRHHGFDIVDFKSLGLLGNLDIGRVPPWRVYRLARSL 209 Query: 188 NTSGVDAIVASACVQMPSLASVQAIEDRTGLPVLSSSVATTYMMLKRL 235 + G DAI S C + ++ + +E TGLPV+SS+ A+ +M LK L Sbjct: 210 SIEGADAIFIS-CTNLRTVEVIGLLERDTGLPVVSSNTASMWMALKTL 256