BLASTP 2.2.21 [Jun-14-2009] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0001.4211.p (268 letters) Database: COGmodified 192,987 sequences; 59,019,183 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value RSc0284 [IQR] COG1028 Dehydrogenases with different specificitie... 253 4e-67 SMc00572 [IQR] COG1028 Dehydrogenases with different specificiti... 109 6e-24 SMc01175 [IQR] COG1028 Dehydrogenases with different specificiti... 107 3e-23 AGc2026 [IQR] COG1028 Dehydrogenases with different specificitie... 106 6e-23 VC1119 [IQR] COG1028 Dehydrogenases with different specificities... 106 7e-23 >RSc0284 [IQR] COG1028 Dehydrogenases with different specificities (related to short-chain alcohol dehydrogenases) Rso Ralstonia solanacearum Length = 266 Score = 253 bits (645), Expect = 4e-67, Method: Compositional matrix adjust. Identities = 149/258 (57%), Positives = 169/258 (65%), Gaps = 3/258 (1%) Query: 13 VFLTGASSGIGMALASAYARRGAVLGLVARRAAELEALRASLPHPERHRIYVLDVTDHAA 72 VF+TGASSGIG ALA YA RGA LGLVARR L +LP Y DV D + Sbjct: 5 VFITGASSGIGQALARQYAARGATLGLVARRVDALHGFVQTLPAGIAVHCYAADVRDARS 64 Query: 73 LAAAAEAFMAEFGMADVVIANAGISQGTLTEYQEDLAAFERILAVNVTATFATFTPFVAR 132 + AA AFMA G+ DVVIANAGIS GT DL AF ++ N T PFV Sbjct: 65 MHDAASAFMAVAGVPDVVIANAGISVGTDLREAGDLPAFAAVMETNWMGVLHTLQPFVGP 124 Query: 133 MKAQPEAQRRRCRLVGIGSVAGIRGLPGAGAYSASKAAVISYCESLRVELNAS---GIKV 189 M A+ E R LVGI SVAG+RGLPGA AYSASKAAVI ESLRVE + G++V Sbjct: 125 MLARAEPGRPGGTLVGIASVAGVRGLPGAAAYSASKAAVIKLMESLRVEFSPKRRPGLRV 184 Query: 190 VTIAPGYIDTPMTRVNSYRMPFLMPVERFAQRAVQSIAEGVSYRVIPWQMGVVAKLLRLL 249 VT+APGYI TPMT N Y MPFLMP +RFA+RAVQ+I G ++V+PWQMGVVA LL +L Sbjct: 185 VTLAPGYIRTPMTEHNPYPMPFLMPADRFAERAVQAIDRGAHFKVLPWQMGVVAGLLHVL 244 Query: 250 PNWLYDMAFARAPHKKRN 267 P WLYD AFA AP K RN Sbjct: 245 PRWLYDFAFAHAPRKPRN 262 >SMc00572 [IQR] COG1028 Dehydrogenases with different specificities (related to short-chain alcohol dehydrogenases) Sme Sinorhizobium meliloti Length = 245 Score = 109 bits (273), Expect = 6e-24, Method: Compositional matrix adjust. Identities = 76/225 (33%), Positives = 120/225 (53%), Gaps = 14/225 (6%) Query: 12 RVFLTGASSGIGMALASAYARRGAVLGLVARRAAELEALRASLPHPERHRIYVLDVTDHA 71 + +TGAS GIG +A +GAV+GL R +LE L +L ER +I+ +++D A Sbjct: 8 KALVTGASGGIGEEIARMLHAQGAVVGLHGTRVEKLETLANTLG--ERVQIFPANLSDRA 65 Query: 72 ALAAAAEAFMAEFGMADVVIANAGISQGTLTEYQEDLAAFERILAVNVTATFATFTPFVA 131 + A E A+ G D+++ NAGI++ L D ++ ++ VN+TA F + Sbjct: 66 EVKALGEKAEADLGGVDILVNNAGITKDGLFVRMSD-EDWDNVIEVNLTAVFR-----LT 119 Query: 132 RMKAQPEAQRRRCRLVGIGSVAGIRGLPGAGAYSASKAAVISYCESLRVELNASGIKVVT 191 R P +RR R+V I SV G+ G PG Y ASKA +I + +SL E+ + V Sbjct: 120 RELTHPMMRRRFGRIVNITSVVGVTGNPGQANYCASKAGMIGFSKSLAQEIATRNVTVNC 179 Query: 192 IAPGYIDTPMT-RVNSYRMPFLM---PVERFAQRAVQSIAEGVSY 232 +APG+I++ MT ++N + +M P+ R A +A V+Y Sbjct: 180 VAPGFIESAMTGKLNDKQKEGIMSAIPMRRMGSGA--EVASAVAY 222 >SMc01175 [IQR] COG1028 Dehydrogenases with different specificities (related to short-chain alcohol dehydrogenases) Sme Sinorhizobium meliloti Length = 282 Score = 107 bits (267), Expect = 3e-23, Method: Compositional matrix adjust. Identities = 79/243 (32%), Positives = 115/243 (47%), Gaps = 10/243 (4%) Query: 14 FLTGASSGIGMALASAYARRGAVLGLVARRAAELEALRASLPHPERHRIYVLDVTDHAAL 73 ++TGASSGIG A+A A G + + AR +L L+ P R + DVTD + Sbjct: 15 WITGASSGIGRAVALRLAEEGYSVVVTARSHEKLVDLQHQAAGPGRMIVLDGDVTDPKDM 74 Query: 74 AAAAEAFMAEFGMADVVIANAGISQGTLTEYQEDL--AAFERILAVNVTATFATFTPFVA 131 E G + + NAG++ L +DL F+R AVN+ P V Sbjct: 75 ERLLAKIEYEHGQVALAVLNAGVA---LPVRGDDLRREPFDRSFAVNLNGVVNCLVPLVE 131 Query: 132 RMKAQPEAQRRRCRLVGIGSVAGIRGLPGAGAYSASKAAVISYCESLRVELNASGIKVVT 191 MK + Q + + SVAG GLP + AY ASKAA+I+ ESL+ +L+ GI++ Sbjct: 132 HMKTKGHGQ-----IAIMSSVAGYGGLPMSAAYGASKAALINMAESLKFDLDRIGIRLQV 186 Query: 192 IAPGYIDTPMTRVNSYRMPFLMPVERFAQRAVQSIAEGVSYRVIPWQMGVVAKLLRLLPN 251 I PG++DT + P L+ + A R + P + + KLLR+LP Sbjct: 187 ICPGFVDTQAAPKGGFPRPALVSTQEAADRICAGLKSSRFEITFPKRFTYLVKLLRILPY 246 Query: 252 WLY 254 LY Sbjct: 247 GLY 249 >AGc2026 [IQR] COG1028 Dehydrogenases with different specificities (related to short-chain alcohol dehydrogenases) Atu Agrobacterium tumefaciens strain C58 (Cereon) Length = 245 Score = 106 bits (264), Expect = 6e-23, Method: Compositional matrix adjust. Identities = 73/225 (32%), Positives = 119/225 (52%), Gaps = 14/225 (6%) Query: 12 RVFLTGASSGIGMALASAYARRGAVLGLVARRAAELEALRASLPHPERHRIYVLDVTDHA 71 + +TGA+ GIG +A ++GA +GL R +LEAL A L +R +I+ ++ D A Sbjct: 8 KALVTGATGGIGEEIARLLHKQGATVGLHGTRVEKLEALAAELG--DRVKIFPANLADRA 65 Query: 72 ALAAAAEAFMAEFGMADVVIANAGISQGTLTEYQEDLAAFERILAVNVTATFATFTPFVA 131 + A E AE D+++ NAGI++ L D ++ ++ VN+TA F + Sbjct: 66 EVKALGEKAEAELEGVDILVNNAGITKDGLFVRMSD-EDWDNVIEVNLTAMFR-----LT 119 Query: 132 RMKAQPEAQRRRCRLVGIGSVAGIRGLPGAGAYSASKAAVISYCESLRVELNASGIKVVT 191 R P +RR R++ I S+ G+ G PG Y ASKA +I + +SL E+ + V Sbjct: 120 RELTHPMMRRRFGRIINITSIVGVTGNPGQANYCASKAGMIGFSKSLAQEIATRNVTVNC 179 Query: 192 IAPGYIDTPMT-RVNSYRMPFLM---PVERFAQRAVQSIAEGVSY 232 +APG+I++ MT ++N + +M P++R A +A V Y Sbjct: 180 VAPGFIESAMTGKLNDKQKDAIMGAIPMKRMGTGA--EVASAVLY 222 >VC1119 [IQR] COG1028 Dehydrogenases with different specificities (related to short-chain alcohol dehydrogenases) Vch Vibrio cholerae Length = 241 Score = 106 bits (264), Expect = 7e-23, Method: Compositional matrix adjust. Identities = 81/240 (33%), Positives = 120/240 (50%), Gaps = 18/240 (7%) Query: 13 VFLTGASSGIGMALASAYARRGAVLGLVARRAAELEALRASLPHPERHRIYVL--DVTDH 70 VF+TGA+SGIG LA YA++G + R + L++L H + I+ L DVTDH Sbjct: 4 VFITGATSGIGKQLALDYAKQGWQVIACGRNQSVLDSL-----HTQYANIFPLAFDVTDH 58 Query: 71 AALAAAAEAFMAEFGMADVVIANAGISQGTLTEYQEDLAAFERILAVNVTATFATFTPFV 130 AA + ++ I NAG + + + + D+ R+ +NV A Sbjct: 59 PGTKAALAQLPCQ---PELWILNAGDCE-YIDDGKMDVTLMARVFNINVLGVAAVIEGI- 113 Query: 131 ARMKAQPEAQRRRCRLVGIGSVAGIRGLPGAGAYSASKAAVISYCESLRVELNASGIKVV 190 QP R+ +GS+A LP A AY ASKAAV +L+++ GI+V Sbjct: 114 -----QPHLSCGH-RVAIVGSIASELALPRAEAYGASKAAVAYLARTLQLDWRPLGIEVT 167 Query: 191 TIAPGYIDTPMTRVNSYRMPFLMPVERFAQRAVQSIAEGVSYRVIPWQMGVVAKLLRLLP 250 TI PG++ TP+T N++ MP ++ VER AQ +A GVS P + + +LL LP Sbjct: 168 TIFPGFVATPLTDRNTFAMPMIITVERAAQEIKAGLARGVSQLYFPKRFTWLIRLLGALP 227