BLASTP 2.2.21 [Jun-14-2009] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0001.4108.p (376 letters) Database: COGmodified 192,987 sequences; 59,019,183 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value YPO3224 [R] COG1073 Hydrolases of the alpha/beta superfamily Ype... 84 6e-16 STM0318 [R] COG1073 Hydrolases of the alpha/beta superfamily Sty... 79 1e-14 VC2276 [R] COG1073 Hydrolases of the alpha/beta superfamily Vch ... 77 5e-14 yafA [R] COG1073 Hydrolases of the alpha/beta superfamily Eco Es... 75 2e-13 BH1839 [R] COG0596 Predicted hydrolases or acyltransferases (alp... 74 7e-13 >YPO3224 [R] COG1073 Hydrolases of the alpha/beta superfamily Ype Yersinia pestis Length = 415 Score = 83.6 bits (205), Expect = 6e-16, Method: Compositional matrix adjust. Identities = 85/290 (29%), Positives = 124/290 (42%), Gaps = 30/290 (10%) Query: 45 GGWCREWIQEAEAAERK----------GRFLL-ASMLYGAARFPCVATPLRAYALQRQVA 93 G W EW + ++K G+F L A+ LY A +P L+ L Q Sbjct: 95 GNWIYEWANQGMMWQQKAMEETDPGSAGQFWLNAANLYSIASYP----HLKGDELSEQAE 150 Query: 94 C-----FERAAPGFPVHFERRYLTCSDSNALTAPVHLYTPRRAEELPLVLLSGGVDTGKM 148 +E AA P + SD +L+ +H+ T A P VL+ GG+DT + Sbjct: 151 VLSNRAYEEAAKYLPYTLKELTFPISDGGSLSGFLHMPTVGSAP-FPTVLMCGGLDTLQS 209 Query: 149 ELHRLACLLCRAGRFRVAAMDMPGT-AESGVALTADSHQLYQHWLDALAP-----SGRKA 202 + HRL + +D+P A S LT D+ L+Q L ALA R + Sbjct: 210 DYHRLFRDYLEPKGIAMLTIDLPSVGASSRWKLTQDTSYLHQQVLQALADVPWVDHQRVS 269 Query: 203 VLGVSFAGHWAAKLALLEAMDA-AVDLGGPTVV-FDSGSAFVHTLPNGMPGIIASALGFT 260 V G F + A +L LE AV GP V S + +P+ ++AS LG Sbjct: 270 VFGFRFGANVAVRLGYLEPQRVRAVACLGPIVHHLLCNSDSLRKVPDMYMDVMASRLGMA 329 Query: 261 RMPDEQSLAALMRPFSLRRQGLLERSGSIPTLVINGERDQYIPQEDSTVL 310 DE +L M +SL+ QGLL R P L E D + P+E++ ++ Sbjct: 330 DSTDE-TLNTEMNRYSLKTQGLLGRRCQTPMLAGFWENDPFSPKEEAKLI 378 >STM0318 [R] COG1073 Hydrolases of the alpha/beta superfamily Sty Salmonella typhimurium LT2 Length = 414 Score = 79.3 bits (194), Expect = 1e-14, Method: Compositional matrix adjust. Identities = 84/287 (29%), Positives = 118/287 (41%), Gaps = 23/287 (8%) Query: 45 GGWCREWIQEA----------EAAERKGRFLL-ASMLYGAARFPCV-ATPLRAYALQRQV 92 G W EW ++A + A R GR+ L AS LY A +P + L A Sbjct: 95 GNWIYEWAKQAMDWQQKACQEQDAMRSGRYWLHASTLYNIAAYPHLKGDELAEQAQALAN 154 Query: 93 ACFERAAPGFPVHFERRYLTCSDSNALTAPVHLYTPRRAEELPLVLLSGGVDTGKMELHR 152 +E AA P + +TA +H+ P+ P VL+ GG+D + + + Sbjct: 155 RAYEEAAQRLPGSLREMEFAVPGGSPVTAFLHM--PKGDGPFPTVLMCGGLDAMQTDYYT 212 Query: 153 LACLLCRAGRFRVAAMDMPGTA-ESGVALTADSHQLYQHWLDAL-----APSGRKAVLGV 206 L + +DMP S LT DS L+QH L AL R A G Sbjct: 213 LYERYFAPRGIAMLTLDMPSVGFSSKWKLTQDSSLLHQHVLKALPNVPWVDHTRVAAFGF 272 Query: 207 SFAGHWAAKLALLEA--MDAAVDLGGPTVVFDSGSAFVHTLPNGMPGIIASALGFTRMPD 264 F + A +LA LEA + A LG S T+P ++AS LG D Sbjct: 273 RFGANVAVRLAYLEAPRLKAVACLGPVVHALLSDPQRQSTVPEMYLDVLASRLGMHDASD 332 Query: 265 EQSLAALMRPFSLRRQGLLERSGSIPTLVINGERDQYIPQEDSTVLA 311 E L R +SL+ QGLL R P L + D + P+E+S ++ Sbjct: 333 EALRVELNR-YSLKVQGLLGRRCPTPMLSGFWKNDPFSPEEESRLIT 378 >VC2276 [R] COG1073 Hydrolases of the alpha/beta superfamily Vch Vibrio cholerae Length = 415 Score = 77.4 bits (189), Expect = 5e-14, Method: Compositional matrix adjust. Identities = 94/344 (27%), Positives = 150/344 (43%), Gaps = 40/344 (11%) Query: 34 SRITDCWHEG-----PGGWCREW-----IQEAEAAERKG-----RFLLASMLYGAARFPC 78 SR D W + G W EW + + +AAER+G + A++ Y A +P Sbjct: 80 SRTHDEWLDTVMGYRSGNWTYEWTRVGMLHQKQAAERQGEEAADQMFAAALYYSIAGYPH 139 Query: 79 VATPLRAYALQRQV---ACFERAAP--GFPVHFERRYLTCSDSNALTAPVHLYTPRRAEE 133 + ALQ QV ++ AA GF V +R + + +HL + Sbjct: 140 LRND--NLALQAQVLANNAYQEAAKLTGFVV---KRLEFSYQNKKIAGYLHLRNTDSPK- 193 Query: 134 LPLVLLSGGVDTGKMELHRLACLLCRAGRFRVAAMDMPGT-AESGVALTADSHQLYQHWL 192 P+VL+S G+D+ + ++ RL + +DMP A S LT DS L+Q L Sbjct: 194 -PVVLVSAGLDSLQTDMWRLFRDYLAKRDIAMLTIDMPSLGASSHWPLTEDSSCLHQAVL 252 Query: 193 DALA-----PSGRKAVLGVSFAGHWAAKLALLEA--MDAAVDLGGPTVVFDSGSAFVHTL 245 + LA R ++G F G+ A+LA LE+ + A V LG P + + + Sbjct: 253 NQLADLPWVDHFRIGLIGFRFGGNAMARLAFLESDKVKACVSLGAPIHDIFTSPNKLAAM 312 Query: 246 PNGMPGIIASALGFTRMPDEQSLAALMRPFSLRRQGLLE-RSGSIPTLVINGERDQYIPQ 304 P ++AS LG + D +SL+ + +SL+ QG + R P L + E D P Sbjct: 313 PKMYLDVLASRLG-KNVVDVRSLSGQLMAWSLKVQGFMSGRRTKTPILALGLEGDPVSPY 371 Query: 305 EDSTVLA-HRPGHQVWLMRGMSHCAAEGLPRILPAMVAWLRLQL 347 D+ ++A G Q + S ++G + L + WL +L Sbjct: 372 SDNQLVALFSQGGQA--KKVKSKTISQGYEQSLDLAINWLEDEL 413 >yafA [R] COG1073 Hydrolases of the alpha/beta superfamily Eco Escherichia coli K12 Length = 414 Score = 75.1 bits (183), Expect = 2e-13, Method: Compositional matrix adjust. Identities = 81/287 (28%), Positives = 116/287 (40%), Gaps = 23/287 (8%) Query: 45 GGWCREWIQEAEAAERK----------GR-FLLASMLYGAARFPCV-ATPLRAYALQRQV 92 G W EW +A ++K GR +L A+ LY A +P + L A Sbjct: 95 GNWIYEWATQAMVWQQKACAEDDPQLSGRHWLHAATLYNIAAYPHLKGDDLAEQAQALSN 154 Query: 93 ACFERAAPGFPVHFERRYLTCSDSNALTAPVHLYTPRRAEELPLVLLSGGVDTGKMELHR 152 +E AA P + T +T +H+ P+ P VL+ GG+D + + + Sbjct: 155 RAYEEAAQRLPGTMRQMEFTVPGGAPITGFLHM--PKGDGPFPTVLMCGGLDAMQTDYYS 212 Query: 153 LACLLCRAGRFRVAAMDMPGTA-ESGVALTADSHQLYQHWLDAL-----APSGRKAVLGV 206 L + +DMP S LT DS L+QH L AL R A G Sbjct: 213 LYERYFAPRGIAMLTIDMPSVGFSSKWKLTQDSSLLHQHVLKALPNVPWVDHTRVAAFGF 272 Query: 207 SFAGHWAAKLALLEA--MDAAVDLGGPTVVFDSGSAFVHTLPNGMPGIIASALGFTRMPD 264 F + A +LA LE+ + A LG S +P ++AS LG D Sbjct: 273 RFGANVAVRLAYLESPRLKAVACLGPVVHTLLSDFKCQQQVPEMYLDVLASRLGMHDASD 332 Query: 265 EQSLAALMRPFSLRRQGLLERSGSIPTLVINGERDQYIPQEDSTVLA 311 E L R +SL+ QGLL R P L + D + P+EDS ++ Sbjct: 333 EALRVELNR-YSLKVQGLLGRRCPTPMLSGYWKNDPFSPEEDSRLIT 378 >BH1839 [R] COG0596 Predicted hydrolases or acyltransferases (alpha/beta hydrolase superfamily) Bha Bacillus halodurans Length = 361 Score = 73.6 bits (179), Expect = 7e-13, Method: Compositional matrix adjust. Identities = 82/337 (24%), Positives = 127/337 (37%), Gaps = 25/337 (7%) Query: 30 RRVRSRITDCWHEGP--GGWCREWIQEAEAAERKGRFLLASMLYGAARFPCVATPLRAYA 87 R+V I E P GGW +W+ A + A Y ARFP T L+ A Sbjct: 26 RQVLQSIERAASEEPTAGGWDWQWVDAANKCLQANAIEDAIQCYNFARFPFPKTSLQQEA 85 Query: 88 LQRQVACFERAAPGFPVHFERRYLTCSDSNALTAPVHLYTPRRAEELPLVLLSGGVDTGK 147 R FE+A V R Y A +H +LP +L+ GG+ + K Sbjct: 86 ADRLTKVFEQAYCREDVQKHRVYYDNQKVTVYAANLH-------PDLPCLLVLGGIVSIK 138 Query: 148 MELHRLACLLCRAGRFRVAAMDMPGTAESGVALTADSHQLYQHWLDAL---APSGRKAVL 204 + + R G F V + PG E+ V ADS+++ LD A G ++ Sbjct: 139 EQWSVFLQVGKRLG-FSVLITECPGVGENQVRYHADSYRMIGQILDTFAGQAKVGDTYIV 197 Query: 205 GVSFAGHWAAKLALL-EAMDAAVDLGGPTVVFDSGSAFVHTLPNGMPGIIASALGFTRMP 263 G+SF GH A K AL E + +G P F F +P I + L Sbjct: 198 GMSFGGHLAMKQALHDERIKGITTVGAPLHYF-----FQDYSQLNVPFITQATLAHVMEE 252 Query: 264 DEQSLAALMRPFSLRRQGLLERSGSIPTLVINGERDQYIPQEDSTVLAHRPGHQVWLMRG 323 + + + F+ + L IP I +D+ IP+ + + L + + Sbjct: 253 ELDHVGRALSRFAFMPKEL--NKLKIPITYIFSSKDEIIPKSEKSFLLDNIANLTFYEFD 310 Query: 324 MSHCAAEGLPRILPAMVAWLRLQLYGSTRTTRALMSL 360 H G P LP + + L + + + R + + Sbjct: 311 DVH----GSPNHLPLIQKIIPLSILKHSNSKRYFVRI 343