BLASTP 2.2.21 [Jun-14-2009] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0001.4065.p (435 letters) Database: COGmodified 192,987 sequences; 59,019,183 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value YPO2280 [NU] COG1450 Type II secretory pathway, component PulD Y... 297 3e-80 mll6829 [NU] COG1450 Type II secretory pathway, component PulD M... 82 2e-15 RSc3114 [NU] COG1450 Type II secretory pathway, component PulD R... 71 6e-12 PA1382 [NU] COG1450 Type II secretory pathway, component PulD Pa... 67 8e-11 gspD [NU] COG1450 Type II secretory pathway, component PulD Eco ... 65 2e-10 >YPO2280 [NU] COG1450 Type II secretory pathway, component PulD Ype Yersinia pestis Length = 414 Score = 297 bits (761), Expect = 3e-80, Method: Compositional matrix adjust. Identities = 163/437 (37%), Positives = 263/437 (60%), Gaps = 47/437 (10%) Query: 1 MKRMITALLMAYSTLAVAAPSGPLLPLTPLSMPLPPGAPPQSDSAMEFSRVRVAEAVEAM 60 MK+++ AL + +S+ A A S +E ++V++ EA+ + Sbjct: 1 MKKILLALTLLFSSCAFAG------------------------SELELNKVKLPEAISLI 36 Query: 61 YTQILKTPYLIQPEVVADERLVSFRFGTGVSARSEVSRFMGLLGLSVRTVNGVDIVGIVK 120 Y+++LK PY++ P++V DER+++FR + R V+R++G + + + T GVD + Sbjct: 37 YSEVLKVPYMLDPQLVNDERMITFRLTPDIDEREFVTRYLGNMNIRIWTKKGVDFIAPYT 96 Query: 121 EAEPDK--EPFVYRPKYRDVTYLVELLRGLFPKGEFTSTRSIHGAPQAITADAATGQSKA 178 EP K + Y P+YR V YL ++L G + G F ++ ++ I+ D+ G S Sbjct: 97 PKEPVKPRYTWTYTPQYRSVAYLSDILGG-YVSGSFNNSGAV------ISDDSLKGSS-- 147 Query: 179 PAPVGSAASLLDTDPDALVFNGTETDIKKLSALLAQLDTRQGEVMVRGQVFEVASNGAEG 238 A++ ++ D LV+ GT+ DI L L+ LDT EV+V G VFEV ++ ++G Sbjct: 148 -----GASNYINRTGDILVYYGTKEDIAILKTLVTSLDTMSDEVVVSGYVFEVQTSQSDG 202 Query: 239 SAFSLALHLLGGTVSAGVSQPSTLDGFVRLKNSSIDAVFAALSSDSRFKTISAPSLRVRS 298 S LA +L + V + LD F+ ++ SIDA+F L +DSRF +SAP LRV++ Sbjct: 203 SGILLAAKILSDKFNISVG-AAGLDNFINIRTGSIDAIFNLLKTDSRFTVVSAPRLRVKN 261 Query: 299 GAAGRFAVGQDVPVLGAISYPGNGSAPVQSVEYRSSGVIFDLRPIVRESVVDLTVGQQLS 358 A+ F+VG DVPVLG+++ N + QSVEYRSSGV+F++ P ++ +DL + QQLS Sbjct: 262 NASASFSVGSDVPVLGSVTV--NNNTTTQSVEYRSSGVLFNVTPSIKSRTMDLKIQQQLS 319 Query: 359 NFIVTQTGVNNSPTLTKREVSTSLSVADGDVVIIGGLAENRESSGRIGFSFLPDWMRSNT 418 NF+ T+TGVNNSPTL KR+V+T +S+ADGD++++GGLAE ++S G+SF S T Sbjct: 320 NFVTTETGVNNSPTLIKRDVTTEVSLADGDIILLGGLAEQKDSKASSGWSFFG----SRT 375 Query: 419 GQNSKTEILLVLQVSRL 435 +++KT+I+++LQV ++ Sbjct: 376 SESNKTDIMVMLQVRKV 392 >mll6829 [NU] COG1450 Type II secretory pathway, component PulD Mlo Mesorhizobium loti Length = 708 Score = 82.4 bits (202), Expect = 2e-15, Method: Compositional matrix adjust. Identities = 71/247 (28%), Positives = 117/247 (47%), Gaps = 15/247 (6%) Query: 176 SKAPAPVGSAASLLDTDPDALVFNGTETDIKKLSALLAQLDTRQGEVMVRGQVFEVASNG 235 ++APA A + D + +AL+ T D +++ +L+++D +VM+ + EV N Sbjct: 397 NQAPA---HATVVADVENNALLIQTTARDYQRIEQILSKVDVLPTQVMLEAVIAEVTLN- 452 Query: 236 AEGSAFSLALHLLGGTVSAGVSQ------PSTLDGF-VRLKNSSIDAVFAALSSDSRFKT 288 + + L G V+ +TL GF +I ALS + Sbjct: 453 -DDLKYGLRWFFENGGTKVSVTDVAKAAAAATLPGFNWSYATDNIQVTLNALSKITDVNV 511 Query: 289 ISAPSLRVRSGAAGRFAVGQDVPVLGAISYP-GNGSAPV-QSVEYRSSGVIFDLRPIVRE 346 ISAP++ + VG VP+L S GNGSAP+ SV+ + +GVI + P + Sbjct: 512 ISAPTIMALNNQKAILQVGDQVPILTQQSQDTGNGSAPIINSVQMKDTGVILTVTPRINN 571 Query: 347 S-VVDLTVGQQLSNFIVTQTGVNNSPTLTKREVSTSLSVADGDVVIIGGLAENRESSGRI 405 + V L + Q++SN T + +SPT+ +R+V T + V DG+ + +GGL + S R Sbjct: 572 AGRVMLDIQQEVSNVTKTDSSDIDSPTIQQRKVQTRVLVNDGESLALGGLIQQNNSVDRS 631 Query: 406 GFSFLPD 412 L D Sbjct: 632 QVPILGD 638 >RSc3114 [NU] COG1450 Type II secretory pathway, component PulD Rso Ralstonia solanacearum Length = 805 Score = 70.9 bits (172), Expect = 6e-12, Method: Compositional matrix adjust. Identities = 77/317 (24%), Positives = 135/317 (42%), Gaps = 61/317 (19%) Query: 171 AATGQSKAPAPVGSAASLLDTDP--DALVFNGTETDIKKLSALLAQLDTRQGEVMVRGQV 228 A+ GQS P + ++ DP +AL+ +E + L ++ LD R+ +V + + Sbjct: 385 ASFGQSNLP----TTGGIIQADPATNALIITASEPVYRNLRTVIDDLDARRAQVYIESMI 440 Query: 229 FEVASNGAE-------------------GSAFS------LALHLLGGTVSAGV------- 256 EV S+ A G+ F L L ++ TV +G Sbjct: 441 VEVTSDKASQLGIQWMVGAGGPNTYGFGGTNFGSGVGNILNLGVIAATVGSGGIGSTAAQ 500 Query: 257 --------SQPSTLDGF---VRLKNSSIDAVFAALSSDSRFKTISAPSLRVRSGAAGRFA 305 S S L+G V KN+ + A+ +AL SD +S P+L + Sbjct: 501 TALGSITGSNVSGLNGGNFGVFNKNTGLGAILSALGSDGSVNVLSTPNLITLDNEEAKIL 560 Query: 306 VGQDVPVLGAISYPGNGSA----PVQSVEYRSSGVIFDLRP-IVRESVVDLTVGQQLSNF 360 +GQ+VP+ SY GS+ P Q+ + + G+ ++P I +V + + Q+ S Sbjct: 561 IGQNVPITTG-SYAQTGSSASVTPFQTFDRKDVGITLRVKPQITDGGMVKMQIFQESSAV 619 Query: 361 IVTQTGVNNSPTLTKREVSTSLSVADGDVVIIGGLAEN--RESSGRI-GFSFLP---DWM 414 + PT R + T++ DG V+++GGL E+ ++S ++ G +P Sbjct: 620 VNGTQNATQGPTTNVRSIETNVIANDGQVIVLGGLLEDNYQDSEQKVPGLGDIPVLGALF 679 Query: 415 RSNTGQNSKTEILLVLQ 431 RS + KT +L+ L+ Sbjct: 680 RSESKTRKKTNLLVFLR 696 >PA1382 [NU] COG1450 Type II secretory pathway, component PulD Pae Pseudomonas aeruginosa Length = 759 Score = 67.0 bits (162), Expect = 8e-11, Method: Compositional matrix adjust. Identities = 58/248 (23%), Positives = 112/248 (45%), Gaps = 20/248 (8%) Query: 183 GSAASLLDTDPDALVFNGTETDIKKLSALLAQLDTRQGEVMVRGQVFEVASNGAEGSAFS 242 GS + D + +A++F G +++ LL ++D +V++ V V+ + + Sbjct: 452 GSGTIVEDENRNAILFRGAARTWQQMQGLLREMDKPARQVLIEVTVASVSLSDTQ--ELG 509 Query: 243 LALHLLGGTVSAGVSQPSTLDGFVRLKNSSID------AVFAALSSDSRFKTISAPSLRV 296 + +L G+ ++ S S N I+ A A++ + R + ++ P + V Sbjct: 510 VEWEMLNGSFNSATSTGSKGSAGKGGFNYVINTAGGNTAAIQAMADNQRVRVLATPRILV 569 Query: 297 RSGAAGRFAVGQDVPV----LGAISYPGNGSAPVQSVEYRSSGVIFDLRPIV-RESVVDL 351 +SG VG+D+P+ + S + + YRS+G I ++ P+V +S VDL Sbjct: 570 KSGEQANINVGRDIPIPTAQVNDDSTTAGSTNLRNEIAYRSTGTILNVAPVVYSDSRVDL 629 Query: 352 TVGQQL-------SNFIVTQTGVNNSPTLTKREVSTSLSVADGDVVIIGGLAENRESSGR 404 TV Q+L G ++P +++ + TSL++ G V++GGL + + Sbjct: 630 TVSQELSDSGGSSGGGGKASGGGISAPEISRTSLETSLTLKSGGSVLMGGLIRDNITDSN 689 Query: 405 IGFSFLPD 412 G L D Sbjct: 690 AGVPLLKD 697 >gspD [NU] COG1450 Type II secretory pathway, component PulD Eco Escherichia coli K12 Length = 654 Score = 65.5 bits (158), Expect = 2e-10, Method: Compositional matrix adjust. Identities = 82/368 (22%), Positives = 143/368 (38%), Gaps = 72/368 (19%) Query: 66 KTPYLIQPEVVADERLVSFRFGTGVSARSEVSRFMGLLGLSVRTVNGVDIVGIVKEAEPD 125 + P L+ ++VAD+R S AR ++ + L V+E+E + Sbjct: 225 QMPALLSAKIVADKRTNSLIISGPEKARQRITSLLKSLD--------------VEESE-E 269 Query: 126 KEPFVYRPKYRDVTYLVELLRGLFPKGEFTSTRSIHGAPQAITADAATGQSKAPAPVG-- 183 VY KY T LVE+L G+ K G ++ P+ G Sbjct: 270 GNTRVYYLKYAKATNLVEVLTGVSEK-----------------LKDEKGNARKPSSSGAM 312 Query: 184 -SAASLLDTDPDALVFNGTETDIKKLSALLAQLDTRQGEVMVRGQVFEVASNGAEGSAFS 242 + A D ++LV ++ +KL+ ++A+LD R+ +V+V + EV +G+ + Sbjct: 313 DNVAITADEQTNSLVITADQSVQEKLATVIARLDIRRAQVLVEAIIVEVQ----DGNGLN 368 Query: 243 LALHLLGGTVSA---------------GVSQPSTLDGFVRLK-----------------N 270 L + V A GV+ G N Sbjct: 369 LGVQWANKNVGAQQFTNTGLPIFNAAQGVADYKKNGGITSANPAWDMFSAYNGMAAGFFN 428 Query: 271 SSIDAVFAALSSDSRFKTISAPSLRVRSGAAGRFAVGQDVPVLGAISYPGNGSAPVQSVE 330 + AL+S+++ ++ PS+ F VGQDVPVL S +G +VE Sbjct: 429 GDWGVLLTALASNNKNDILATPSIVTLDNKLASFNVGQDVPVLSG-SQTTSGDNVFNTVE 487 Query: 331 YRSSGVIFDLRPIVRESVVDLTVGQQLSNFIVTQTGVNNSPTLTKREVSTSLSVADGDVV 390 ++ G + P V E L +Q + + + + PT R + ++ V G+ V Sbjct: 488 RKTVGTKLKVTPQVNEGDAVLLEIEQEVSSVDSSSNSTLGPTFNTRTIQNAVLVKTGETV 547 Query: 391 IIGGLAEN 398 ++GGL ++ Sbjct: 548 VLGGLLDD 555