BLASTP 2.2.21 [Jun-14-2009] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0001.3864.p (286 letters) Database: COGmodified 192,987 sequences; 59,019,183 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value VC1074 [R] COG1266 Predicted metal-dependent membrane protease V... 103 5e-22 Z1787 [R] COG1266 Predicted metal-dependent membrane protease Ec... 97 6e-20 ECs1085 [R] COG1266 Predicted metal-dependent membrane protease ... 97 6e-20 YPO1140 [R] COG1266 Predicted metal-dependent membrane protease ... 96 7e-20 PA5132 [R] COG1266 Predicted metal-dependent membrane protease P... 83 6e-16 >VC1074 [R] COG1266 Predicted metal-dependent membrane protease Vch Vibrio cholerae Length = 276 Score = 103 bits (256), Expect = 5e-22, Method: Compositional matrix adjust. Identities = 90/263 (34%), Positives = 130/263 (49%), Gaps = 10/263 (3%) Query: 24 RLHLALWLPLLVLAVVTGVVDGRIQAMGVIGLLGLGVAAWGAARASVSRQRRCLLILALL 83 R H W LL++ +++ + G + +G G++ +G A A S R + LL Sbjct: 23 RYHRVSW-GLLIITLLSALGSGYLTFVGA-GIICVGFALAYLAANGQSHWRTTAWVALLL 80 Query: 84 MSLLLALHRWPGFINVQVLPRQAITPDALPFLLYANFDKGAAGLLLLALLVPRCQRLREW 143 L L LH PGF N++VL + + + PF LY N DK LL + + Sbjct: 81 WCLALFLHWLPGFSNLKVLDKVIASAHSTPFSLYLNLDKPLVFFALLLAFPALLGQSK-- 138 Query: 144 STTLRLTLLPGFVTIATVMLLGWLIGMVRPEWKWPPFAGIFLAINLLLTVVAEEAFFRGL 203 + ++ TLL A + + WL G + EW P + IF NLL T VAEEA FRGL Sbjct: 139 TANIKATLLTLVPLFALLPIAVWL-GALAFEWSLPEWWWIFALNNLLFTCVAEEALFRGL 197 Query: 204 IQYRMQAALQHLRAGDLLAVLVSALLFGAAHLGGGLGYAALATVAGLGYAWVFRRSARIE 263 IQ + Q + G + +L+++ LFG AH GG A +AGLGY VF + R+ Sbjct: 198 IQQKAQQ-----QFGAIAGLLIASALFGMAHFAGGPLLMIFAALAGLGYGLVFHFTGRLW 252 Query: 264 SAIALHFTLNAVHFIGFTYPALA 286 ++ +HF N H + FTYP LA Sbjct: 253 VSVGVHFLFNFAHLLFFTYPMLA 275 >Z1787 [R] COG1266 Predicted metal-dependent membrane protease EcZ Escherichia coli O157:H7 EDL933 Length = 274 Score = 96.7 bits (239), Expect = 6e-20, Method: Compositional matrix adjust. Identities = 67/205 (32%), Positives = 101/205 (49%), Gaps = 8/205 (3%) Query: 82 LLMSLLLALHRWPGFINVQVLPRQAITPDALPFLLYANFDKGAAGLLLLALLVPRCQRLR 141 +L ++ L H WPGF N VL + P + P+ +Y NFDK LL+ L + Sbjct: 74 VLSAIALFFHLWPGFHNPVVLNSVTVGPQSTPYTMYFNFDKALVPFLLV--LCTSSLFKK 131 Query: 142 EWSTTLRLTLLPGF-VTIATVMLLGWLIGMVRPEWKWPPFAGIFLAINLLLTVVAEEAFF 200 E + + L +++ ++ L G ++PE +P + F+ NL +AEE+ F Sbjct: 132 EVKSEVSLWKWGALSLSVPLILFLAVFFGGLKPEIHFPEWLPEFILANLFFVSLAEESLF 191 Query: 201 RGLIQYRMQAALQHLRAGDLLAVLVSALLFGAAHLGGGLGYAALATVAGLGYAWVFRRSA 260 RG IQ R+ L+A++V+ALLFG H GG AT++G+ Y + S Sbjct: 192 RGYIQSRLSEV-----TSPLVALIVAALLFGFYHYSGGALLVLFATLSGVVYGLSWMWSG 246 Query: 261 RIESAIALHFTLNAVHFIGFTYPAL 285 R+ A HF LN H + FTYP L Sbjct: 247 RLWVATLFHFGLNLCHLLFFTYPFL 271 >ECs1085 [R] COG1266 Predicted metal-dependent membrane protease Ecs Escherichia coli O157:H7 Length = 272 Score = 96.7 bits (239), Expect = 6e-20, Method: Compositional matrix adjust. Identities = 67/205 (32%), Positives = 101/205 (49%), Gaps = 8/205 (3%) Query: 82 LLMSLLLALHRWPGFINVQVLPRQAITPDALPFLLYANFDKGAAGLLLLALLVPRCQRLR 141 +L ++ L H WPGF N VL + P + P+ +Y NFDK LL+ L + Sbjct: 72 VLSAIALFFHLWPGFHNPVVLNSVTVGPQSTPYTMYFNFDKALVPFLLV--LCTSSLFKK 129 Query: 142 EWSTTLRLTLLPGF-VTIATVMLLGWLIGMVRPEWKWPPFAGIFLAINLLLTVVAEEAFF 200 E + + L +++ ++ L G ++PE +P + F+ NL +AEE+ F Sbjct: 130 EVKSEVSLWKWGALSLSVPLILFLAVFFGGLKPEIHFPEWLPEFILANLFFVSLAEESLF 189 Query: 201 RGLIQYRMQAALQHLRAGDLLAVLVSALLFGAAHLGGGLGYAALATVAGLGYAWVFRRSA 260 RG IQ R+ L+A++V+ALLFG H GG AT++G+ Y + S Sbjct: 190 RGYIQSRLSEV-----TSPLVALIVAALLFGFYHYSGGALLVLFATLSGVVYGLSWMWSG 244 Query: 261 RIESAIALHFTLNAVHFIGFTYPAL 285 R+ A HF LN H + FTYP L Sbjct: 245 RLWVATLFHFGLNLCHLLFFTYPFL 269 >YPO1140 [R] COG1266 Predicted metal-dependent membrane protease Ype Yersinia pestis Length = 272 Score = 96.3 bits (238), Expect = 7e-20, Method: Compositional matrix adjust. Identities = 69/196 (35%), Positives = 107/196 (54%), Gaps = 6/196 (3%) Query: 88 LALHRWPGFINVQVLPRQAITPDALPFLLYANFDKGAAGLLLLALLVPRCQRLREWSTTL 147 L LH PGF N++VL + P + PF +Y N DK +LLA L P ++++ + Sbjct: 78 LFLHLVPGFHNLKVLDKVRTGPLSAPFTMYYNLDKALVPFILLACL-PTLFKVKKHPSVG 136 Query: 148 RLTLLPGFVTIATVMLLGWLIGMVRPEWKWPPFAGIFLAINLLLTVVAEEAFFRGLIQYR 207 R+ + +++ ++LL +G ++ E P + G F+ NL +AEEA FRG +Q R Sbjct: 137 RMGWVVLILSVPALLLLAVALGGLKIELHTPVWIGSFIIANLFFVCLAEEALFRGYLQQR 196 Query: 208 MQAALQHLRAGDLLAVLVSALLFGAAHLGGGLGYAALATVAGLGYAWVFRRSARIESAIA 267 + L G A++++ALLFG+AH GG A +AG+ Y + S R+ A+A Sbjct: 197 LGQWL-----GSYPALVITALLFGSAHFAGGPLLMLFAALAGVIYGLAWLWSGRLWVAVA 251 Query: 268 LHFTLNAVHFIGFTYP 283 HF LN +H + FTYP Sbjct: 252 FHFALNLMHLLFFTYP 267 >PA5132 [R] COG1266 Predicted metal-dependent membrane protease Pae Pseudomonas aeruginosa Length = 272 Score = 83.2 bits (204), Expect = 6e-16, Method: Compositional matrix adjust. Identities = 71/196 (36%), Positives = 96/196 (48%), Gaps = 6/196 (3%) Query: 88 LALHRWPGFINVQVLPRQAITPDALPFLLYANFDKGAAGLLLLALLVPRCQRLREWSTTL 147 L LH PGF N +L + + P +LPF +Y NFDK LLLA L P R + Sbjct: 78 LFLHLLPGFANPSILDKAVVGPQSLPFTMYFNFDKALVPFLLLACL-PSLFRDEARAPGR 136 Query: 148 RLTLLPGFVTIATVMLLGWLIGMVRPEWKWPPFAGIFLAINLLLTVVAEEAFFRGLIQYR 207 L + ++LL +G++RPE P + F+ NL +AEEA FRG +Q R Sbjct: 137 PWHWLLLVAAVPALLLLAVGVGLLRPELHAPAWLWQFILANLFFVSLAEEALFRGYLQQR 196 Query: 208 MQAALQHLRAGDLLAVLVSALLFGAAHLGGGLGYAALATVAGLGYAWVFRRSARIESAIA 267 + L G A+ +++ LFG AH GG A +AGL Y + S R+ A Sbjct: 197 LGQWL-----GPWPALALASALFGLAHFAGGPLLMLFAGLAGLIYGLAWLWSGRLWVATL 251 Query: 268 LHFTLNAVHFIGFTYP 283 HF LN H + FTYP Sbjct: 252 FHFGLNLTHLLLFTYP 267