BLASTP 2.2.21 [Jun-14-2009] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0001.3846.p (255 letters) Database: COGmodified 192,987 sequences; 59,019,183 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value RSp0209 [Q] COG5285 Protein involved in biosynthesis of mitomyci... 237 2e-62 Rv1501 [Q] COG5285 Protein involved in biosynthesis of mitomycin... 47 5e-05 MT1550 [Q] COG5285 Protein involved in biosynthesis of mitomycin... 47 5e-05 PA1218 Non COG 45 1e-04 mlr7444 Non COG 44 5e-04 >RSp0209 [Q] COG5285 Protein involved in biosynthesis of mitomycin antibiotics/polyketide fumonisin Rso Ralstonia solanacearum Length = 263 Score = 237 bits (605), Expect = 2e-62, Method: Compositional matrix adjust. Identities = 130/249 (52%), Positives = 158/249 (63%) Query: 2 SNPLSPAQVAFFREQGYLLLPDMVPADLREQMLAVTQDHLQRAVAPLEYEAEVGYAGAPA 61 S+ L Q AFF GYL+L + +LA + L AV P+E EA+VGYAGAPA Sbjct: 11 SHALDAEQQAFFHRHGYLILRALAGPSECAALLAAARAALDAAVPPVELEADVGYAGAPA 70 Query: 62 SLEAEGGRTARRLRAAWQRDPVYRQWAGDPQLVAMLHQLLEEPVCITLAHHNCVMTKHPA 121 S +A GGRT RRL A R + WA D ++ + QL+ PV ++LAHHNCVMTKHPA Sbjct: 71 SRDAVGGRTVRRLLDADSRGTPFTDWARDARVATPIAQLIGAPVHLSLAHHNCVMTKHPA 130 Query: 122 FGTATGWHRDIRYWSFPRNELISVWLALGAETPENGALKFIPGSHKLKLQPEQMDELDFL 181 FGTAT WHRD RYWSF RNEL+S WLAL ET +NG L IPGSH L L + D+ FL Sbjct: 131 FGTATHWHRDSRYWSFARNELVSAWLALVEETVDNGCLWVIPGSHTLDLSAGRFDDKLFL 190 Query: 182 RPDVPANQALFEQGIALSLDPGDVVLFHSGLFHAAGRNDSDQVKCSAVFAYHGKSNPPLP 241 R D+P N+AL + + L PGDV+LFH FHAA N + +K S V Y+G N PLP Sbjct: 191 RQDLPENRALIDTARPVPLLPGDVLLFHCNTFHAARANQTGAIKFSLVTTYYGTDNTPLP 250 Query: 242 GTRSAASED 250 GTRS+A D Sbjct: 251 GTRSSARPD 259 >Rv1501 [Q] COG5285 Protein involved in biosynthesis of mitomycin antibiotics/polyketide fumonisin Mtu Mycobacterium tuberculosis H37Rv Length = 273 Score = 46.6 bits (109), Expect = 5e-05, Method: Compositional matrix adjust. Identities = 52/214 (24%), Positives = 90/214 (42%), Gaps = 41/214 (19%) Query: 17 GYLLLPDMVPADLREQMLAVTQDHLQRAVAPLEYEAEVGYAGAPASLEAEGGRTARRLRA 76 GY ++ D+ L E LA T+D + R + E+G A G LR Sbjct: 23 GYAVVEDV----LDEASLAATRDRMYRVQERIL--TEIG-----KERLARAGELGV-LRL 70 Query: 77 AWQRDPVYRQWAGDPQLVAMLHQLLEEPVCITLAHHNCVMTKHPAFGT----ATGWHRDI 132 + DP + + P++++++ ++L E + L + ++ P F T +H+D Sbjct: 71 MMKYDPHFFTFLEIPEVLSIVDRVLSETAILHL-QNGFILPSFPPFSTPDVFQNAFHQD- 128 Query: 133 RYWSFPRN-----ELISVWLALGAETPENGALKFIPGSHKLKLQPEQMDELDFLRPDVPA 187 FPR +++ A+ T + GA +PGSH+ R + P Sbjct: 129 ----FPRVLSGYIASVNIMFAIDPFTRDTGATLVVPGSHQ--------------RIEKPD 170 Query: 188 NQALFEQGIALSLDPGDVVLFHSGLFHAAGRNDS 221 + L + + G + +F S L+HAAGRN S Sbjct: 171 HTYLARNAVPVQCAAGSLFVFDSTLWHAAGRNTS 204 >MT1550 [Q] COG5285 Protein involved in biosynthesis of mitomycin antibiotics/polyketide fumonisin MtC Mycobacterium tuberculosis CDC1551 Length = 273 Score = 46.6 bits (109), Expect = 5e-05, Method: Compositional matrix adjust. Identities = 52/214 (24%), Positives = 90/214 (42%), Gaps = 41/214 (19%) Query: 17 GYLLLPDMVPADLREQMLAVTQDHLQRAVAPLEYEAEVGYAGAPASLEAEGGRTARRLRA 76 GY ++ D+ L E LA T+D + R + E+G A G LR Sbjct: 23 GYAVVEDV----LDEASLAATRDRMYRVQERIL--TEIG-----KERLARAGELGV-LRL 70 Query: 77 AWQRDPVYRQWAGDPQLVAMLHQLLEEPVCITLAHHNCVMTKHPAFGT----ATGWHRDI 132 + DP + + P++++++ ++L E + L + ++ P F T +H+D Sbjct: 71 MMKYDPHFFTFLEIPEVLSIVDRVLSETAILHL-QNGFILPSFPPFSTPDVFQNAFHQD- 128 Query: 133 RYWSFPRN-----ELISVWLALGAETPENGALKFIPGSHKLKLQPEQMDELDFLRPDVPA 187 FPR +++ A+ T + GA +PGSH+ R + P Sbjct: 129 ----FPRVLSGYIASVNIMFAIDPFTRDTGATLVVPGSHQ--------------RIEKPD 170 Query: 188 NQALFEQGIALSLDPGDVVLFHSGLFHAAGRNDS 221 + L + + G + +F S L+HAAGRN S Sbjct: 171 HTYLARNAVPVQCAAGSLFVFDSTLWHAAGRNTS 204 >PA1218 Non COG Length = 292 Score = 45.1 bits (105), Expect = 1e-04, Method: Compositional matrix adjust. Identities = 49/204 (24%), Positives = 83/204 (40%), Gaps = 14/204 (6%) Query: 24 MVPADLREQMLAVTQDHLQRAVAPLEYEAEVGYAGAPASLEAEGGRTARRLRAAWQRDPV 83 +V LR+Q L QR LE E ++ + R A+++R + DP+ Sbjct: 7 VVSPQLRQQALDWILAAAQRPDPALEPEFDIA---------DDERREAKKVRNLHRHDPL 57 Query: 84 Y-RQWAGDPQLVAMLHQLLEEPVCITLAHHNCVMTKHPAFGTATGWHRDIRYWSFPRNEL 142 + W L A++ L +P L H + +H A + H+DI W Sbjct: 58 FWDDWFARSGLDALVRDHLRKP---RLLRHAAFIKRH-ADESYIPLHQDIALWEKRYESA 113 Query: 143 ISVWLALGAETPENGALKFIPGSHKLKLQPEQMDELDFLRPDVPANQALFEQGIALSLDP 202 + W+AL +NG + + P + + F D+ AN EQ + LD Sbjct: 114 QTFWVALTPSRNDNGGMFYYPDDRTIFPHEFDLAYPMFKCIDLQANGISREQLVDAELDA 173 Query: 203 GDVVLFHSGLFHAAGRNDSDQVKC 226 GDV+++ + H + N S Q++ Sbjct: 174 GDVLVWPARTAHGSHNNRSGQLRI 197 >mlr7444 Non COG Length = 273 Score = 43.5 bits (101), Expect = 5e-04, Method: Compositional matrix adjust. Identities = 32/121 (26%), Positives = 52/121 (42%), Gaps = 19/121 (15%) Query: 112 HNCVMTKHPAFGTATGWHRDIRYWSFPRNELISVWLALGAETPENGALKFIPGSH---KL 168 H+ V+ K P T WH+DI Y+ + +S W+ + + + L+ I GSH K+ Sbjct: 106 HDHVLVKEPGTQKPTPWHQDIPYYFVDGRQTVSFWIPI--DPVKEATLRLIAGSHKWDKM 163 Query: 169 KLQPEQMDELDFL----------RPDVPANQALFEQGIALSLDPGDVVLFHSGLFHAAGR 218 L +D+ +F PD + + E ++PGD +LF H A Sbjct: 164 ILPVRWLDDSNFYAGEGDYLPVPDPDKDPSMKVLE----WEMEPGDAILFDFRTAHGARG 219 Query: 219 N 219 N Sbjct: 220 N 220