BLASTP 2.2.21 [Jun-14-2009] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0001.3771.p (397 letters) Database: COGmodified 192,987 sequences; 59,019,183 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value RSc2871 [R] COG1473 Metal-dependent amidase/aminoacylase/carboxy... 560 e-159 SMc02256 [R] COG1473 Metal-dependent amidase/aminoacylase/carbox... 387 e-107 PA4344 [R] COG1473 Metal-dependent amidase/aminoacylase/carboxyp... 384 e-106 AGl2386 [R] COG1473 Metal-dependent amidase/aminoacylase/carboxy... 379 e-105 AGl849 [R] COG1473 Metal-dependent amidase/aminoacylase/carboxyp... 377 e-104 >RSc2871 [R] COG1473 Metal-dependent amidase/aminoacylase/carboxypeptidase Rso Ralstonia solanacearum Length = 396 Score = 560 bits (1444), Expect = e-159, Method: Compositional matrix adjust. Identities = 262/394 (66%), Positives = 312/394 (79%), Gaps = 1/394 (0%) Query: 1 MKLIDPIVQFQSELQQIRRDIHAHPELAYEEVRTADVVAQKLTEWGIPVVRGLGVTGVVG 60 MKLI I+ Q+E+Q +RRDIHAHPEL +EE RT+D+VA KL EWGI V RGLG TG+VG Sbjct: 1 MKLIPEILAAQAEIQALRRDIHAHPELCFEEQRTSDLVAAKLAEWGIEVHRGLGKTGLVG 60 Query: 61 IIKQGDSPRAIGLRADMDALPMPEINTFAHASRHAGKMHACGHDGHTAMLLGAAYYLARH 120 +I+ G+ R IGLRADMDALP+ E N F H SRH GKMHACGHDGHTAMLLGAA+YLARH Sbjct: 61 VIRNGEGQR-IGLRADMDALPLAEANQFTHRSRHEGKMHACGHDGHTAMLLGAAHYLARH 119 Query: 121 RNFDGTVYVIFQPAEEGGRGAERMIQDGLFEQYPMDAVFGMHNWPGIPAGHFGVTPGAQM 180 RNF GTV++IFQPAEEGG GA MI+DGLF+++P DAVFGMHNWPG+P G FG G M Sbjct: 120 RNFSGTVHLIFQPAEEGGGGAREMIRDGLFDRFPCDAVFGMHNWPGVPVGAFGTRVGPLM 179 Query: 181 ASSNEFHVTVKGRGSHAAQPHKAADPVMTAVHIAQAWQSIVARNINPNDPAVVSITQIHT 240 ASSNEF + +KG+G+HAA PH DPV + A Q ++ RN P D AV+SITQ H Sbjct: 180 ASSNEFRIVIKGKGAHAALPHNGNDPVFVGAQMVSALQGVITRNKRPIDTAVLSITQFHA 239 Query: 241 GSATNVIPDEAMMVGTVRTFSLPVLDLIERRMKEIAEHTAAAFDATVDFRFNRNYPPLIN 300 G A+N+IP+EA + GTVRTFS VLDLIERRM+E+A+ AAA+D ++DF F+RNYPP +N Sbjct: 240 GDASNIIPNEAWIGGTVRTFSTAVLDLIERRMEEVAKAIAAAYDCSIDFTFHRNYPPTVN 299 Query: 301 HAKETAFAVEVLTEQFGADRVDAHTEPTMGAEDFAFMLQHKPGCYVFLGNGDGGHRDQGH 360 +ET FA EV+ E G D VDA+ +PTMGAEDF+FML KPGC+ F+GNGDG HR+QGH Sbjct: 300 TERETLFAAEVMRELVGPDHVDANIDPTMGAEDFSFMLIEKPGCFAFIGNGDGDHREQGH 359 Query: 361 GLGPCNLHNPSYDFNDDLLPIGATYWVRLAEKFL 394 GLGPC LHNPSYDFND+LLP+GATYWVRL EKFL Sbjct: 360 GLGPCMLHNPSYDFNDELLPLGATYWVRLVEKFL 393 >SMc02256 [R] COG1473 Metal-dependent amidase/aminoacylase/carboxypeptidase Sme Sinorhizobium meliloti Length = 389 Score = 387 bits (993), Expect = e-107, Method: Compositional matrix adjust. Identities = 195/394 (49%), Positives = 253/394 (64%), Gaps = 8/394 (2%) Query: 1 MKLIDPIVQFQSELQQIRRDIHAHPELAYEEVRTADVVAQKLTEWGIPVVRGLGVTGVVG 60 MKL I EL IRRD+HAHPEL EE RT+ +A+ L E G V G+ TGVVG Sbjct: 1 MKLSAAIDNAMPELVAIRRDLHAHPELGLEETRTSAFIARHLEELGYEVATGIAKTGVVG 60 Query: 61 IIKQGDSPRAIGLRADMDALPMPEINTFAHASRHAGKMHACGHDGHTAMLLGAAYYLARH 120 ++ G R+IG+RAD+DALP+ E A+AS G MHACGHDGHTAMLLGAA LA Sbjct: 61 TLRNGTGSRSIGIRADIDALPIQEETGVAYASTKPGLMHACGHDGHTAMLLGAARALAER 120 Query: 121 RNFDGTVYVIFQPAEEGGRGAERMIQDGLFEQYPMDAVFGMHNWPGIPAGHFGVTPGAQM 180 RNFDGT+++IFQPAEE GA+ M+ +GLF+++P DAVF +HN P +P G F + G M Sbjct: 121 RNFDGTIHLIFQPAEENAGGAKIMVDEGLFDRFPCDAVFALHNEPNLPFGQFALREGPIM 180 Query: 181 ASSNEFHVTVKGRGSHAAQPHKAADPVMTAVHIAQAWQSIVARNINPNDPAVVSITQIHT 240 A+ +E +TV GRG H A+P ADP++ I A Q+IVARNI+P DP+VV++ H Sbjct: 181 AAVDEARITVHGRGGHGAEPQATADPIVCGASIVMALQTIVARNIHPMDPSVVTVGAFHA 240 Query: 241 GSATNVIPDEAMMVGTVRTFSLPVLDLIERRMKEIAEHTAAAFDATVDFRFNRNYPPLIN 300 GSA+N+IP+ A +V +R+F V D +ERR++ IAE A++F + R+Y IN Sbjct: 241 GSASNIIPERAEIVVGIRSFDPAVRDELERRIRMIAEAQASSFGMRATVDYERSYDATIN 300 Query: 301 HAKETAFAVEVLTEQFGADRVDAHTEPTMGAEDFAFMLQHKPGCYVFLGNGDGGHRDQGH 360 H ET F E GAD+V P MG+EDFA+ML+ +PG Y FLG+ G Sbjct: 301 HKAETDFLREAAIRFAGADKVVDLARPFMGSEDFAYMLKERPGSYFFLGSRVTGEEK--- 357 Query: 361 GLGPCNLHNPSYDFNDDLLPIGATYWVRLAEKFL 394 +LH+P YDFNDDLLPIGA +W LAE +L Sbjct: 358 -----SLHHPGYDFNDDLLPIGAAFWTELAEAYL 386 >PA4344 [R] COG1473 Metal-dependent amidase/aminoacylase/carboxypeptidase Pae Pseudomonas aeruginosa Length = 406 Score = 384 bits (985), Expect = e-106, Method: Compositional matrix adjust. Identities = 191/388 (49%), Positives = 253/388 (65%), Gaps = 10/388 (2%) Query: 9 QFQSELQQIRRDIHAHPELAYEEVRTADVVAQKLTEWGIPVVRGLGVTGVVGIIKQGDSP 68 + +L+ +R+DIHAHPEL +EE RTA +VA+ L WG V G+G TGVVG+++QGD Sbjct: 13 EVADDLRSLRQDIHAHPELGFEERRTAALVAECLRGWGYEVHEGIGRTGVVGVLRQGDGT 72 Query: 69 RAIGLRADMDALPMPEINTFAHASRHAGKMHACGHDGHTAMLLGAAYYLARHRNFDGTVY 128 R +GLRADMDALP+ E ++S H G+MHACGHDGHTAMLLGAA YLA R FDGT+ Sbjct: 73 RRLGLRADMDALPIVEATGLGYSSCHGGRMHACGHDGHTAMLLGAARYLAATRRFDGTLV 132 Query: 129 VIFQPAEEGGRGAERMIQDGLFEQYPMDAVFGMHNWPGIPAGHFGVTPGAQMASSNEFHV 188 +IFQPAEEG GAE M+ DGL E++P DA+FGMHN PG+ AGH G G MAS + V Sbjct: 133 LIFQPAEEGQGGAEAMLADGLLERFPCDALFGMHNMPGLEAGHLGFRAGPMMASQDLLSV 192 Query: 189 TVKGRGSHAAQPHKAADPVMTAVHIAQAWQSIVARNINPNDPAVVSITQIHTGSATNVIP 248 T++G G H + PH + DP++ A A QS+VARN++P AVV++ + G A NVIP Sbjct: 193 TLEGVGGHGSMPHLSVDPLLAASSAVMALQSVVARNVDPQKAAVVTVGALQAGEAANVIP 252 Query: 249 DEAMMVGTVRTFSLPVLDLIERRMKEIAEHTAAAFDATVDFRFNRNYPPLINHAKETAFA 308 A++ ++R V + + +R+++I E AA++ YP L+N +ET FA Sbjct: 253 QRAVLRLSLRALDGQVREQVLQRVRQIIELQAASYGCQASIEHYPAYPVLVNSVEETEFA 312 Query: 309 VEVLTEQFGADRVDAHTEPTMGAEDFAFMLQHKPGCYVFLGNGDGGHRDQGHGLGPCNLH 368 +V E GA++VD T MG+EDFA+MLQ PG Y+F+GNG G +H Sbjct: 313 RQVGVELAGAEQVDGDTPKLMGSEDFAWMLQRCPGSYLFIGNGR----------GRPMVH 362 Query: 369 NPSYDFNDDLLPIGATYWVRLAEKFLQA 396 NP+YDFNDD+L GA YW LAE +L A Sbjct: 363 NPAYDFNDDILVRGAAYWGALAETWLAA 390 >AGl2386 [R] COG1473 Metal-dependent amidase/aminoacylase/carboxypeptidase Atu Agrobacterium tumefaciens strain C58 (Cereon) Length = 430 Score = 379 bits (972), Expect = e-105, Method: Compositional matrix adjust. Identities = 193/397 (48%), Positives = 257/397 (64%), Gaps = 15/397 (3%) Query: 1 MKLIDPIVQFQSELQQIRRDIHAHPELAYEEVRTADVVAQKLTEWGI-PVVRGLGVTGVV 59 M +++ + Q E+ + R +H +PE+ Y+ TA VA+KL +G+ +V GLG TGVV Sbjct: 44 MPILNRAAELQQEVSEWRHHLHENPEILYDVDNTAAFVAEKLKSFGVDEIVTGLGRTGVV 103 Query: 60 GIIKQGDSP--RAIGLRADMDALPMPEINTFAHASRHAGKMHACGHDGHTAMLLGAAYYL 117 GII+ G+ P R IG RADMDALP+ E AS+ AG MHACGHDGHTAMLLGAA YL Sbjct: 104 GIIR-GNVPGNRTIGFRADMDALPILEETGKPWASKTAGAMHACGHDGHTAMLLGAAKYL 162 Query: 118 ARHRNFDGTVYVIFQPAEEGGRGAERMIQDGLFEQYPMDAVFGMHNWPGIPAGHFGVTPG 177 RNF G++ VIFQPAEEGG GA M++DGL E++ +D V+GMHN PGIP G F V G Sbjct: 163 TETRNFAGSIAVIFQPAEEGGAGALAMVEDGLMERFGIDEVYGMHNMPGIPLGAFAVRKG 222 Query: 178 AQMASSNEFHVTVKGRGSHAAQPHKAADPVMTAVHIAQAWQSIVARNINPNDPAVVSITQ 237 MA+ ++F +TVKGRG HAAQPH+ DP+ I Q I +RN +P VVS+T+ Sbjct: 223 GIMAAPDKFSITVKGRGGHAAQPHRTIDPITIGAQIVGNLQMIASRNADPIRSVVVSVTR 282 Query: 238 IHTGSATNVIPDEAMMVGTVRTFSLPVLDLIERRMKEIAEHTAAAFDATVDFRFNRNYPP 297 H GS+ N+IP+EA++ GTVR+ V DL + R+K++AE A A A + + R P Sbjct: 283 FHAGSSHNIIPNEALIAGTVRSLDETVRDLAQERIKQMAEGIALANGAEAEVVYERYCPV 342 Query: 298 LINHAKETAFAVEVLTEQFGADRVDAHTEPTMGAEDFAFMLQHKPGCYVFLGNGDGGHRD 357 NH+ ET +AV V + G VD +P+M EDF+FML+ +PGC++F+GNGD Sbjct: 343 TFNHSAETDYAVRVARDVVGEPNVDPDVDPSMAGEDFSFMLKERPGCFIFIGNGDS---- 398 Query: 358 QGHGLGPCNLHNPSYDFNDDLLPIGATYWVRLAEKFL 394 LHNPSYDFND+ + G +YWV+LAE+ L Sbjct: 399 -------AGLHNPSYDFNDEAIAYGISYWVKLAEQRL 428 >AGl849 [R] COG1473 Metal-dependent amidase/aminoacylase/carboxypeptidase Atu Agrobacterium tumefaciens strain C58 (Cereon) Length = 374 Score = 377 bits (967), Expect = e-104, Method: Compositional matrix adjust. Identities = 191/382 (50%), Positives = 248/382 (64%), Gaps = 15/382 (3%) Query: 18 RRDIHAHPELAYEEVRTADVVAQKLTEWGIPVVR-GLGVTGVVGIIK--QGDSPRAIGLR 74 RR +H HPEL Y+ T+ VA+KLT +G VV G+G TGVVGIIK GD P IG R Sbjct: 5 RRHLHEHPELLYDVFETSKFVAEKLTSFGCDVVETGIGKTGVVGIIKGRHGDGP-TIGFR 63 Query: 75 ADMDALPMPEINTFAHASRHAGKMHACGHDGHTAMLLGAAYYLARHRNFDGTVYVIFQPA 134 +DMDALP+ E + AS+ GK H+CGHDGHTAMLLGAA YLA RNF G++ VIFQPA Sbjct: 64 SDMDALPILETSGKPWASKVPGKAHSCGHDGHTAMLLGAAQYLAETRNFKGSIAVIFQPA 123 Query: 135 EEGGRGAERMIQDGLFEQYPMDAVFGMHNWPGIPAGHFGVTPGAQMASSNEFHVTVKGRG 194 EEGG GA M+ DG+ E++ + V+GMHN PGIP G F + G+ MA+++ F + + G+G Sbjct: 124 EEGGAGALAMLNDGMMEKFGISQVYGMHNEPGIPVGQFAIRKGSTMAAADSFEIVITGKG 183 Query: 195 SHAAQPHKAADPVMTAVHIAQAWQSIVARNINPNDPAVVSITQIHTGSATNVIPDEAMMV 254 SHAA PH + DPV+T+ +I A QSIV+R +P VV++ H G+A NVIP + Sbjct: 184 SHAAAPHLSVDPVLTSAYIIIALQSIVSRETDPLKSLVVTVATTHGGTAVNVIPGSVTLT 243 Query: 255 GTVRTFSLPVLDLIERRMKEIAEHTAAAFDATVDFRFNRNYPPLINHAKETAFAVEVLTE 314 GTVRT + E+R+KE+A TA A AT + ++ R YP NHA ET FA V Sbjct: 244 GTVRTLLPETRNFAEKRLKEVATATAMAHGATAEVKYRRGYPVTFNHADETEFATGVAMG 303 Query: 315 QFGADRVDAHTEPTMGAEDFAFMLQHKPGCYVFLGNGDGGHRDQGHGLGPCNLHNPSYDF 374 GA+ V+ + P MGAEDF++ML+ +PG ++F+GNGD LHN +YDF Sbjct: 304 VAGANAVNTNPNPHMGAEDFSYMLEARPGAFIFIGNGD-----------TAGLHNAAYDF 352 Query: 375 NDDLLPIGATYWVRLAEKFLQA 396 NDD LP G +YWV +AEK L A Sbjct: 353 NDDALPYGISYWVSMAEKALAA 374