BLASTP 2.2.21 [Jun-14-2009] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0001.3673.p (242 letters) Database: COGmodified 192,987 sequences; 59,019,183 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value PH1150 [Q] COG3473 Maleate cis-trans isomerase Pho Pyrococcus ho... 89 1e-17 APE2520 [Q] COG3473 Maleate cis-trans isomerase Ape Aeropyrum pe... 85 1e-16 PAB2420 [Q] COG3473 Maleate cis-trans isomerase Pab Pyrococcus a... 84 2e-16 SSO2939 [Q] COG3473 Maleate cis-trans isomerase Sso Sulfolobus s... 81 2e-15 PH0985 [Q] COG3473 Maleate cis-trans isomerase Pho Pyrococcus ho... 79 1e-14 >PH1150 [Q] COG3473 Maleate cis-trans isomerase Pho Pyrococcus horikoshii Length = 236 Score = 88.6 bits (218), Expect = 1e-17, Method: Compositional matrix adjust. Identities = 65/222 (29%), Positives = 111/222 (50%), Gaps = 8/222 (3%) Query: 4 RTLLGMLTPSSNTTLEPVTTAMLADIPEA-SAHFGRFRVTEIALSNQALAQFDDSEILRA 62 R +G++ PSSNTT+E + L PE S H R + I +++ L + + S + A Sbjct: 5 RGRIGLIVPSSNTTMEMELHSFL---PEGVSLHTSRMPLKSI--TDEELFKMN-SYAIEA 58 Query: 63 AELLSHAKVGSIAWNGTSSGWLGF-DADERLCRRITEATGIAACTSVLALNEIFDLTGVK 121 A LL+ A V IA+ +S ++G D ++ L +I E I T+ A+ E D+ + Sbjct: 59 ASLLADAGVEVIAFGCSSGSFIGGQDFEKELEMKIEEEVNIETFTTSTAVLEALDVLDAQ 118 Query: 122 RFGLVTPYLDEVQAAIIANYAASGLECVSERHLRKQDNFSFSEVGAEELRAMVREVAKDK 181 +VTPY DE+ +G E + + L DN ++ + + + + Sbjct: 119 SLLIVTPYSDEINQKEKEFLEGNGFEVLDIKGLNLTDNLEIGKLEPYVVYRLAKAMFAGD 178 Query: 182 PQAITIFCTNLRGAPLVEELEREVGIPIYDTISTVVWKSLQQ 223 AI I CTNLR ++E+LER++G+P+ + +W +L+Q Sbjct: 179 ADAIFISCTNLRTFEIIEKLERDLGVPVITSNQATLWMALRQ 220 >APE2520 [Q] COG3473 Maleate cis-trans isomerase Ape Aeropyrum pernix Length = 272 Score = 85.1 bits (209), Expect = 1e-16, Method: Compositional matrix adjust. Identities = 61/224 (27%), Positives = 108/224 (48%), Gaps = 8/224 (3%) Query: 4 RTLLGMLTPSSNTTLEPVTTAMLADIPEA-SAHFGRFRVTEIALSNQALAQFDDSEILRA 62 R +G++ PSSNTT+EP M PE S H GR + ++++ + L + ++ + A Sbjct: 40 RARIGLIVPSSNTTMEP---EMWMMAPEGVSIHTGRVPLEKVSV--EELLKMEEYSAIEA 94 Query: 63 AELLSHAKVGSIAWNGTSSGWLGFDA-DERLCRRITEATGIAACTSVLALNEIFDLTGVK 121 E L+ A V I + T+ +G D ++ +++ A+G+ T+ A+ E G Sbjct: 95 -EKLATAGVDLIVYGCTTGSLIGGPGYDLKIADKLSSASGVQVVTTATAVVEALKALGAS 153 Query: 122 RFGLVTPYLDEVQAAIIANYAASGLECVSERHLRKQDNFSFSEVGAEELRAMVREVAKDK 181 R + TPY++EV G + V + L N V + + R ++ + Sbjct: 154 RIAVATPYIEEVNKREEEFLRHHGFDIVDFKSLGLLGNLDIGRVPPWRVYRLARSLSIEG 213 Query: 182 PQAITIFCTNLRGAPLVEELEREVGIPIYDTISTVVWKSLQQAG 225 AI I CTNLR ++ LER+ G+P+ + + +W +L+ G Sbjct: 214 ADAIFISCTNLRTVEVIGLLERDTGLPVVSSNTASMWMALKTLG 257 >PAB2420 [Q] COG3473 Maleate cis-trans isomerase Pab Pyrococcus abyssi Length = 236 Score = 84.3 bits (207), Expect = 2e-16, Method: Compositional matrix adjust. Identities = 66/225 (29%), Positives = 110/225 (48%), Gaps = 14/225 (6%) Query: 4 RTLLGMLTPSSNTTLEPVTTAMLADIPEA-SAHFGRF---RVTEIALSNQALAQFDDSEI 59 R +G++ PSSNTT+E ++L PE S H R VTE L N + S Sbjct: 5 RGRIGLIVPSSNTTMEMEFHSVL---PEGVSLHTSRMPLKSVTEEELLNMS------SYA 55 Query: 60 LRAAELLSHAKVGSIAWNGTSSGWLG-FDADERLCRRITEATGIAACTSVLALNEIFDLT 118 + AA+LL+ A V IA+ TS ++G D ++ L +I + + T+ A+ E ++ Sbjct: 56 IEAAKLLADAGVEVIAFGCTSGSFIGGKDFEKELEMKIEKEVNVETFTTSTAVIEALNIL 115 Query: 119 GVKRFGLVTPYLDEVQAAIIANYAASGLECVSERHLRKQDNFSFSEVGAEELRAMVREVA 178 ++ ++TPY+DE+ A+G E V R L DN ++ + + Sbjct: 116 EMQTVQVITPYIDEINQREKEFLEANGFEVVDIRGLGISDNIEIGKLEPYIAYRLAKASF 175 Query: 179 KDKPQAITIFCTNLRGAPLVEELEREVGIPIYDTISTVVWKSLQQ 223 I I CTN R ++E+LER++G+P+ + +W +L+Q Sbjct: 176 TGDVDGIFISCTNFRTFEIIEKLERDLGVPVVTSNQATLWMALRQ 220 >SSO2939 [Q] COG3473 Maleate cis-trans isomerase Sso Sulfolobus solfataricus Length = 242 Score = 80.9 bits (198), Expect = 2e-15, Method: Compositional matrix adjust. Identities = 69/240 (28%), Positives = 120/240 (50%), Gaps = 14/240 (5%) Query: 7 LGMLTPSSNTTLEPVTTAMLADIPEA--SAHFGRFRVTEIALSNQALAQFDDSEILRAAE 64 +G++ PSSNTT+E + + I E + HF R ++ + + + LA+ + E RAA Sbjct: 6 VGVIIPSSNTTVEYEFSNVFNQIKEYRITPHFSRIKLRNVTIED--LAEME-RETERAAS 62 Query: 65 LLSHAKVGSIAWNGTS-SGWLGFDADERLCRRITEATGI-AACTSVLALNEIFDLTGVKR 122 LS I++ T+ S + G E + +RI + + A TS +N + L +KR Sbjct: 63 ELSTILPQIISYACTTGSLFKGPKHHEEIIQRIEKIAKVPATATSGSVINALRRL-DIKR 121 Query: 123 FGLVTPYLDEVQAAIIANYAASGLECVSERHLRKQDNFSFSEVGAEELR----AMVREVA 178 L+TPY++EV I ++ +G E V + ++N +V EE+ ++R V Sbjct: 122 LVLLTPYIEEVNKKEIEFFSKNGFEIVKSAGMGIRENIKIGQVTPEEVYNFAVNVLRNVK 181 Query: 179 KDKPQAITIFCTNLRGAPLVEELEREVGIPIYDTISTVVWKSLQQAGADPSRIRGWGSLF 238 D + I CTNLR ++ LE+E P+ + S +W++L + + ++R G LF Sbjct: 182 GDY-DGVFISCTNLRTFEILSRLEKETKKPVISSNSATLWETLYKINLN-IKVRELGHLF 239 >PH0985 [Q] COG3473 Maleate cis-trans isomerase Pho Pyrococcus horikoshii Length = 238 Score = 79.0 bits (193), Expect = 1e-14, Method: Compositional matrix adjust. Identities = 64/242 (26%), Positives = 118/242 (48%), Gaps = 15/242 (6%) Query: 4 RTLLGMLTPSSNTTLEPVTTAMLADIPEA-SAHFGRFRVTEIALSNQALAQFDDSEILRA 62 R +G++ PSSNTT+EP M PE S H R R+ E+ + ++L + + A Sbjct: 5 RRRIGLIVPSSNTTMEPEFWKM---APEGVSIHTARMRLREV--TEESLLEMERHAKDAA 59 Query: 63 AELLSHAKVGSIAWNGTSSGWL-GFDADERLCRRITEATGIAACTSVLALNEIFDLTGVK 121 EL + A+V I + TS + G D+ + + + EA+GI T+ A+ + ++ Sbjct: 60 LEL-ADAEVDVIVYGCTSGSLIKGKGYDKEIAKNLEEASGIKTITTSTAVLNALNTLDIQ 118 Query: 122 RFGLVTPYLDEVQAAIIANYAASGLECVSERHLRKQDNFSFSEVGAEE---LRAMVREVA 178 + + TPY+D V A+G+E + + L N +E+G +E + EV Sbjct: 119 KVVVATPYIDSVNEREKEFLEANGIEVLRIKGLGIVKN---TEIGRQEPWVAYKLALEVY 175 Query: 179 KDKPQAITIFCTNLRGAPLVEELEREVGIPIYDTISTVVWKSLQQAGADPSRIRGWGSLF 238 + + I CTN R ++++LE E+G+P+ + +W +L+ + +G+L Sbjct: 176 TPEADGLFISCTNFRTIEIIDKLEVELGVPVVTSNQATMWYALKTMKVK-EKYDMYGTLL 234 Query: 239 RD 240 ++ Sbjct: 235 KE 236